UniProtKB - P21401 (GP_RVFVZ)
Envelopment polyprotein
GP
Functioni
Structural component of the virion that interacts with glycoprotein C (PubMed:18715915).
It shields the hydrophobic fusion loops of the glycoprotein C, preventing premature fusion (PubMed:29367607).
The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (PubMed:18715915, PubMed:29367607).
They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (PubMed:21767814).
Plays a role in the packaging of ribonucleoproteins and polymerase during virus assembly (PubMed:21445316).
4 PublicationsStructural component of the virion that interacts with glycoprotein N (PubMed:18715915).
Acts as a class II fusion protein that is activated upon acidification and subsequent repositioning of the glycoprotein N (PubMed:29367607).
The glycoprotein protrusions are arranged on an icosahedral lattice, with T=12 triangulation (PubMed:18715915, PubMed:29367607).
They are able to attach the virion to the host cell receptor CD209/DC-SIGN and to promote fusion of membranes with the late endosome after endocytosis of the virion (PubMed:21767814).
3 PublicationsPlays a role in the inhibition of virus-induced apoptosis. Plays a role for virus dissemination in vertebrates.
2 PublicationsPlays a role for virus dissemination in mosquitoes. May act as a structural virion protein in insects.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 1194 | Important for glycoprotein C and glycoprotein N subcellular locationBy similarity | 1 |
GO - Biological processi
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- modulation by virus of host apoptotic process Source: UniProtKB-KW
- suppression by virus of host apoptotic process Source: UniProtKB
- viral budding from Golgi membrane Source: UniProtKB
- viral entry into host cell Source: UniProtKB-KW
- virion assembly Source: UniProtKB
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Names & Taxonomyi
Protein namesi | Recommended name: Envelopment polyproteinAlternative name(s): M polyprotein Cleaved into the following 3 chains: NSm-Gn protein1 Publication Alternative name(s): p78 Alternative name(s): Glycoprotein G1 Alternative name(s): Glycoprotein G2 |
Gene namesi | Name:GP |
Organismi | Rift valley fever virus (strain ZH-548 M12) (RVFV) |
Taxonomic identifieri | 11589 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Negarnaviricota › Polyploviricotina › Ellioviricetes › Bunyavirales › Phenuiviridae › Phlebovirus › |
Virus hosti | Aedes [TaxID: 7158] Bos taurus (Bovine) [TaxID: 9913] Bos taurus x Bison bison (beefalo) [TaxID: 297284] Camelus bactrianus (Bactrian camel) [TaxID: 9837] Capra hircus (Goat) [TaxID: 9925] Homo sapiens (Human) [TaxID: 9606] Ovis aries (Sheep) [TaxID: 9940] Phlebotomus papatasi (Sandfly) [TaxID: 29031] |
Proteomesi |
|
Subcellular locationi
- Virion membrane By similarity; Single-pass type I membrane protein By similarity
- Host Golgi apparatus membrane 2 Publications; Single-pass type I membrane protein By similarity
- Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.By similarity
- Virion membrane By similarity; Single-pass type I membrane protein By similarity
- Host Golgi apparatus membrane 1 Publication; Single-pass type I membrane protein By similarity Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein C to the Golgi complex, where virion budding occurs.By similarity
- Host mitochondrion outer membrane 1 Publication; Single-pass type II membrane protein 1 Publication
- Host Golgi apparatus 1 Publication
- Virion 1 Publication Note: Localizes in virions maturing in cells of insect origin but not in cells of mammalian origin.1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 17 – 130 | Cytoplasmic1 PublicationAdd BLAST | 114 | |
Topological domaini | 154 – 582 | LumenalSequence analysisAdd BLAST | 429 | |
Transmembranei | 583 – 603 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 604 – 673 | CytoplasmicBy similarityAdd BLAST | 70 | |
Topological domaini | 691 – 1159 | LumenalSequence analysisAdd BLAST | 469 | |
Transmembranei | 1160 – 1180 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1181 – 1197 | CytoplasmicBy similarityAdd BLAST | 17 |
Keywords - Cellular componenti
Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Host mitochondrion, Host mitochondrion outer membrane, Membrane, VirionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 16 | Sequence analysisAdd BLAST | 16 | |
ChainiPRO_0000247010 | 17 – 1197 | Envelopment polyproteinAdd BLAST | 1181 | |
ChainiPRO_0000434914 | 17 – 690 | NSm-Gn proteinAdd BLAST | 674 | |
ChainiPRO_0000036851 | 154 – 690 | Glycoprotein NSequence analysisAdd BLAST | 537 | |
ChainiPRO_0000036852 | 691 – 1197 | Glycoprotein CSequence analysisAdd BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 179 ↔ 188 | By similarity | ||
Disulfide bondi | 229 ↔ 239 | By similarity | ||
Disulfide bondi | 250 ↔ 281 | By similarity | ||
Disulfide bondi | 271 ↔ 284 | By similarity | ||
Disulfide bondi | 304 ↔ 456 | By similarity | ||
Disulfide bondi | 322 ↔ 332 | By similarity | ||
Disulfide bondi | 374 ↔ 434 | By similarity | ||
Disulfide bondi | 402 ↔ 413 | By similarity | ||
Disulfide bondi | 420 ↔ 425 | By similarity | ||
Disulfide bondi | 479 ↔ 482 | By similarity | ||
Disulfide bondi | 486 ↔ 556 | By similarity | ||
Disulfide bondi | 506 ↔ 511 | By similarity | ||
Disulfide bondi | 691 ↔ 731 | 1 Publication | ||
Disulfide bondi | 704 ↔ 713 | 1 Publication | ||
Disulfide bondi | 756 ↔ 852 | 1 Publication | ||
Disulfide bondi | 771 ↔ 965 | 1 Publication | ||
Disulfide bondi | 777 ↔ 825 | 1 Publication | ||
Disulfide bondi | 783 ↔ 832 | 1 Publication | ||
Disulfide bondi | 788 ↔ 814 | 1 Publication | ||
Glycosylationi | 794 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Disulfide bondi | 818 ↔ 823 | 1 Publication | ||
Disulfide bondi | 934 ↔ 947 | 1 Publication | ||
Disulfide bondi | 1029 ↔ 1101 | 1 Publication | ||
Glycosylationi | 1035 | N-linked (GlcNAc...) asparagine; by hostBy similarity | 1 | |
Disulfide bondi | 1039 ↔ 1042 | 1 Publication | ||
Disulfide bondi | 1049 ↔ 1083 | 1 Publication | ||
Glycosylationi | 1077 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 153 – 154 | Cleavage; by host signal peptidase1 Publication | 2 | |
Sitei | 690 – 691 | Cleavage; by host signal peptidase1 Publication | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinInteractioni
Subunit structurei
Heterodimer with glycoprotein C (By similarity). Homotrimer (postfusion) (By similarity).
Interacts with nucleocapsid protein N and with the polymerase L in order to package them into virus particles (PubMed:21445316).
Interacts with host E3 ubiquitin-protein ligase UBR4; this interaction is important for viral RNA production (PubMed:35032865).
By similarity2 PublicationsStructurei
Secondary structure
3D structure databases
SMRi | P21401 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 131 – 153 | Internal signal sequence for glycoprotein N1 PublicationAdd BLAST | 23 | |
Regioni | 608 – 650 | Golgi retention signalBy similarityAdd BLAST | 43 | |
Regioni | 646 – 650 | Important for correct targeting of the glycoproteins to the Golgi complex but not for heterodimerizationBy similarity | 5 | |
Regioni | 675 – 690 | Internal signal sequence for glycoprotein CBy similarityAdd BLAST | 16 | |
Regioni | 777 – 783 | Fusion loopBy similarity | 7 | |
Regioni | 819 – 830 | Fusion loop1 PublicationAdd BLAST | 12 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixFamily and domain databases
DisProti | DP02493 |
InterProi | View protein in InterPro IPR016404, M_polyprot_prcur_phlebovir IPR043603, Phlebo_G2_C IPR010826, Phlebovirus_G1 IPR009878, Phlebovirus_G2_fusion IPR009879, Phlebovirus_NSM |
Pfami | View protein in Pfam PF19019, Phlebo_G2_C, 1 hit PF07243, Phlebovirus_G1, 1 hit PF07245, Phlebovirus_G2, 1 hit PF07246, Phlebovirus_NSM, 2 hits |
PIRSFi | PIRSF003961, M_poly_PhleboV, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MYVLLTILTS VLVCEAIIRV SLSSTREETC FGDSTNPEMI EGAWDSLREE
60 70 80 90 100
EMPEELSCSI SGIREVKTSS QELYRALKAI IAADGLNNIT CHGKDPEDKI
110 120 130 140 150
SLIKGPPHKK RVGIVRCERR RDAKQIGRKT MAGIAMTVLP ALAVFALAPV
160 170 180 190 200
VFAEDPHLRN RPGKGHNYID GMTQEDATCK PVTYAGACSS FDVLLEKGKF
210 220 230 240 250
PLFQSYAHHR TLLEAVHDTI IAKADPPSCD LLSAHGNPCM KEKLVMKTHC
260 270 280 290 300
PNDYQSAHHL NNDGKMASVK CPPKYELTED CNFCRQMTGA SLKKGSYPLQ
310 320 330 340 350
DLFCQSSEDD GSKLKTKMKG VCEVGVQALK KCDGQLSTAH EVVPFAVFKN
360 370 380 390 400
SKKVYLDKLD LKTEENLLPD SFVCFEHKGQ YKGTMDSGQT KRELKSFDIS
410 420 430 440 450
QCPKIGGHGS KKCTGDAAFC SAYECTAQYA NAYCSHANGS GIVQIQVSGV
460 470 480 490 500
WKKPLCVGYE RVVVKRELSA KPIQRVEPCT TCITKCEPHG LVVRSTGFKI
510 520 530 540 550
SSAVACASGV CVTGSQSPST EITLKYPGIS QSSGGDIGVH MAHDDQSVSS
560 570 580 590 600
KIVAHCPPQD PCLVHDCIVC AHGLINYQCH TALSAFVVVF VFSSIAIICL
610 620 630 640 650
AILYRVLKCL KIAPRKVLNP LMWITAFIRW IYKKMVARVA DNINQVNREI
660 670 680 690 700
GWMEGGQLVL GNPAPIPRHA PIPRYSTYLM LLLIVSYASA CSELIQASSR
710 720 730 740 750
ITTCSTEGVN TKCRLSGTAL IRAGSVGAEA CLMLKGVKED QTKFLKLKTV
760 770 780 790 800
SSELSCREGQ SYWTGSFSPK CLSSRRCHLV GECHVNRCLS WRDNETSAEF
810 820 830 840 850
SFVGESTTMR ENKCFEQCGG WGCGCFNVNP SCLFVHTYLQ SVRKEALRVF
860 870 880 890 900
NCIDWVHKLT LEITDFDGSV STIDLGASSS RFTNWGSVSL SLDAEGISGS
910 920 930 940 950
NSFSFIESPG KGYAIVDEPF SEIPRQGFLG EIRCNSESSV LSAHESCLRA
960 970 980 990 1000
PNLISYKPMI DQLECTTNLI DPFVVFERGS LPQTRNDKTF AASKGNRGVQ
1010 1020 1030 1040 1050
AFSKGSVQAD LTLMFDNFEV DFVGAAVSCD AAFLNLTGCY SCNAGARVCL
1060 1070 1080 1090 1100
SITSTGTGSL SAHNKDGSLH IVLPSENGTK DQCQILHFTV PEVEEEFMYS
1110 1120 1130 1140 1150
CDGDERPLLV KGTLIAIDPF DDRREAGGES TVVNPKSGSW NFFDWFSGLM
1160 1170 1180 1190
SWFGGPLKTI LLICLYVALS IGLFFLLIYL GGTGLSKMWL AATKKAS
The sequence of this isoform differs from the canonical sequence as follows:
1-38: Missing.
154-1197: Missing.
The sequence of this isoform differs from the canonical sequence as follows:
1-51: Missing.
154-1197: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 9 | T → I in ABD38819 (PubMed:17192303).Curated | 1 | |
Sequence conflicti | 17 | I → V in ABD38819 (PubMed:17192303).Curated | 1 | |
Sequence conflicti | 129 | K → E in ABD38819 (PubMed:17192303).Curated | 1 | |
Sequence conflicti | 259 | H → Y in ABD38819 (PubMed:17192303).Curated | 1 | |
Sequence conflicti | 747 | L → I in ABD38819 (PubMed:17192303).Curated | 1 | |
Sequence conflicti | 1182 | G → R in ABD38819 (PubMed:17192303).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057988 | 1 – 51 | Missing in isoform NSm' protein. Add BLAST | 51 | |
Alternative sequenceiVSP_057989 | 1 – 38 | Missing in isoform NSm protein. Add BLAST | 38 | |
Alternative sequenceiVSP_057990 | 154 – 1197 | Missing in isoform NSm protein and isoform NSm' protein. Add BLAST | 1044 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25276 Genomic RNA Translation: AAA47449.1 DQ380206 Genomic RNA Translation: ABD38819.1 |
PIRi | A30183, VGVURF |
RefSeqi | YP_003848705.1, NC_014396.1 |
Genome annotation databases
GeneIDi | 9538296 |
KEGGi | vg:9538296 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25276 Genomic RNA Translation: AAA47449.1 DQ380206 Genomic RNA Translation: ABD38819.1 |
PIRi | A30183, VGVURF |
RefSeqi | YP_003848705.1, NC_014396.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6F8P | X-ray | 1.60 | A | 154-469 | [»] | |
6F9B | electron microscopy | 13.30 | A/C/E/G/I/K/M/O/Q/S/U/X | 154-469 | [»] | |
B/D/F/H/J/L/N/P/R/T/V/Y | 691-1118 | [»] | ||||
6F9C | electron microscopy | 8.00 | A/C/E/G/I/K | 154-469 | [»] | |
B/D/F/H/J/L | 691-1118 | [»] | ||||
6F9D | electron microscopy | 13.30 | A/C/E/G/I/K | 154-469 | [»] | |
B/D/F/H/J/L | 691-1118 | [»] | ||||
6F9E | electron microscopy | 13.30 | A/C/E/G/I/K | 154-469 | [»] | |
B/D/F/H/J/L | 691-1118 | [»] | ||||
6F9F | electron microscopy | 13.30 | A/C/E/G/I | 154-469 | [»] | |
B/D/F/H/J | 691-1118 | [»] | ||||
6I9I | X-ray | 1.98 | C/D | 154-469 | [»] | |
SMRi | P21401 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protocols and materials databases
ABCDi | P21401, 3 sequenced antibodies |
Genome annotation databases
GeneIDi | 9538296 |
KEGGi | vg:9538296 |
Family and domain databases
DisProti | DP02493 |
InterProi | View protein in InterPro IPR016404, M_polyprot_prcur_phlebovir IPR043603, Phlebo_G2_C IPR010826, Phlebovirus_G1 IPR009878, Phlebovirus_G2_fusion IPR009879, Phlebovirus_NSM |
Pfami | View protein in Pfam PF19019, Phlebo_G2_C, 1 hit PF07243, Phlebovirus_G1, 1 hit PF07245, Phlebovirus_G2, 1 hit PF07246, Phlebovirus_NSM, 2 hits |
PIRSFi | PIRSF003961, M_poly_PhleboV, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GP_RVFVZ | |
Accessioni | P21401Primary (citable) accession number: P21401 Secondary accession number(s): A2T075 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 100 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families