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Protein

Proton/glutamate-aspartate symporter

Gene

gltP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the proton-dependent, binding-protein-independent transport of glutamate and aspartate.4 Publications

Activity regulationi

Glutamate uptake is inhibited by L-cysteate and beta-hydroxyaspartate. Inhibited by the uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP).3 Publications

Kineticsi

    1. Vmax=2.6 nmol/min/mg enzyme with glutamate as substrate1 Publication
    2. Vmax=2.3 nmol/min/mg enzyme with aspartate as substrate1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • aspartate transmembrane transport Source: InterPro
    • dicarboxylic acid transport Source: EcoCyc
    • L-glutamate transmembrane transport Source: InterPro

    Keywordsi

    Biological processAmino-acid transport, Symport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTP-MONOMER
    MetaCyc:GLTP-MONOMER

    Protein family/group databases

    TCDBi2.A.23.1.1 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proton/glutamate-aspartate symporterUniRule annotationCurated
    Alternative name(s):
    Glutamate-aspartate carrier proteinCurated
    Proton-glutamate-aspartate transport protein1 Publication
    Gene namesi
    Name:gltP2 PublicationsUniRule annotation
    Ordered Locus Names:b4077, JW4038
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10405 gltP

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 5CytoplasmicCurated5
    Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
    Topological domaini27 – 50PeriplasmicCuratedAdd BLAST24
    Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
    Topological domaini72 – 84CytoplasmicCuratedAdd BLAST13
    Transmembranei85 – 105HelicalSequence analysisAdd BLAST21
    Topological domaini106 – 159PeriplasmicCuratedAdd BLAST54
    Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
    Topological domaini181 – 210CytoplasmicCuratedAdd BLAST30
    Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
    Topological domaini232PeriplasmicCurated1
    Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
    Topological domaini254 – 292CytoplasmicCuratedAdd BLAST39
    Transmembranei293 – 313HelicalSequence analysisAdd BLAST21
    Topological domaini314 – 324PeriplasmicCuratedAdd BLAST11
    Transmembranei325 – 345HelicalSequence analysisAdd BLAST21
    Topological domaini346 – 361CytoplasmicCuratedAdd BLAST16
    Transmembranei362 – 382HelicalSequence analysisAdd BLAST21
    Topological domaini383 – 387PeriplasmicCurated5
    Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
    Topological domaini409 – 437Cytoplasmic1 PublicationAdd BLAST29

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-UniRule
    • plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002020881 – 437Proton/glutamate-aspartate symporterAdd BLAST437

    Proteomic databases

    PaxDbiP21345
    PRIDEiP21345

    Interactioni

    Protein-protein interaction databases

    BioGridi4262679, 10 interactors
    STRINGi316385.ECDH10B_4268

    Structurei

    3D structure databases

    ProteinModelPortaliP21345
    SMRiP21345
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. GltP subfamily. [View classification]UniRule annotationCurated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105CK2 Bacteria
    COG1301 LUCA
    HOGENOMiHOG000208778
    InParanoidiP21345
    KOiK11102
    OMAiEKAWWIS
    PhylomeDBiP21345

    Family and domain databases

    Gene3Di1.10.3860.10, 1 hit
    HAMAPiMF_02063 GltP_subfam, 1 hit
    InterProiView protein in InterPro
    IPR034703 GltP
    IPR033380 GltP/GltT
    IPR001991 Na-dicarboxylate_symporter
    IPR018107 Na-dicarboxylate_symporter_CS
    IPR036458 Na:dicarbo_symporter_sf
    PANTHERiPTHR42865:SF4 PTHR42865:SF4, 1 hit
    PfamiView protein in Pfam
    PF00375 SDF, 1 hit
    SUPFAMiSSF118215 SSF118215, 1 hit
    PROSITEiView protein in PROSITE
    PS00713 NA_DICARBOXYL_SYMP_1, 1 hit
    PS00714 NA_DICARBOXYL_SYMP_2, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P21345-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKNIKFSLAW QILFAMVLGI LLGSYLHYHS DSRDWLVVNL LSPAGDIFIH
    60 70 80 90 100
    LIKMIVVPIV ISTLVVGIAG VGDAKQLGRI GAKTIIYFEV ITTVAIILGI
    110 120 130 140 150
    TLANVFQPGA GVDMSQLATV DISKYQSTTE AVQSSSHGIM GTILSLVPTN
    160 170 180 190 200
    IVASMAKGEM LPIIFFSVLF GLGLSSLPAT HREPLVTVFR SISETMFKVT
    210 220 230 240 250
    HMVMRYAPVG VFALIAVTVA NFGFSSLWPL AKLVLLVHFA ILFFALVVLG
    260 270 280 290 300
    IVARLCGLSV WILIRILKDE LILAYSTASS ESVLPRIIEK MEAYGAPVSI
    310 320 330 340 350
    TSFVVPTGYS FNLDGSTLYQ SIAAIFIAQL YGIDLSIWQE IILVLTLMVT
    360 370 380 390 400
    SKGIAGVPGV SFVVLLATLG SVGIPLEGLA FIAGVDRILD MARTALNVVG
    410 420 430
    NALAVLVIAK WEHKFDRKKA LAYEREVLGK FDKTADQ
    Length:437
    Mass (Da):47,159
    Last modified:March 1, 1992 - v2
    Checksum:i62E1A5FC16EA54BE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32488 Genomic DNA Translation: AAA23832.1 Sequence problems.
    M84805 Genomic DNA Translation: AAA24323.1
    U00006 Genomic DNA Translation: AAC43171.1
    U00096 Genomic DNA Translation: AAD13460.1
    AP009048 Genomic DNA Translation: BAE78079.1
    PIRiA42384
    RefSeqiNP_418501.1, NC_000913.3
    WP_000789592.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAD13460; AAD13460; b4077
    BAE78079; BAE78079; BAE78079
    GeneIDi948591
    KEGGiecj:JW4038
    eco:b4077
    PATRICifig|1411691.4.peg.2626

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M32488 Genomic DNA Translation: AAA23832.1 Sequence problems.
    M84805 Genomic DNA Translation: AAA24323.1
    U00006 Genomic DNA Translation: AAC43171.1
    U00096 Genomic DNA Translation: AAD13460.1
    AP009048 Genomic DNA Translation: BAE78079.1
    PIRiA42384
    RefSeqiNP_418501.1, NC_000913.3
    WP_000789592.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP21345
    SMRiP21345
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262679, 10 interactors
    STRINGi316385.ECDH10B_4268

    Protein family/group databases

    TCDBi2.A.23.1.1 the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

    Proteomic databases

    PaxDbiP21345
    PRIDEiP21345

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD13460; AAD13460; b4077
    BAE78079; BAE78079; BAE78079
    GeneIDi948591
    KEGGiecj:JW4038
    eco:b4077
    PATRICifig|1411691.4.peg.2626

    Organism-specific databases

    EchoBASEiEB0400
    EcoGeneiEG10405 gltP

    Phylogenomic databases

    eggNOGiENOG4105CK2 Bacteria
    COG1301 LUCA
    HOGENOMiHOG000208778
    InParanoidiP21345
    KOiK11102
    OMAiEKAWWIS
    PhylomeDBiP21345

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTP-MONOMER
    MetaCyc:GLTP-MONOMER

    Miscellaneous databases

    PROiPR:P21345

    Family and domain databases

    Gene3Di1.10.3860.10, 1 hit
    HAMAPiMF_02063 GltP_subfam, 1 hit
    InterProiView protein in InterPro
    IPR034703 GltP
    IPR033380 GltP/GltT
    IPR001991 Na-dicarboxylate_symporter
    IPR018107 Na-dicarboxylate_symporter_CS
    IPR036458 Na:dicarbo_symporter_sf
    PANTHERiPTHR42865:SF4 PTHR42865:SF4, 1 hit
    PfamiView protein in Pfam
    PF00375 SDF, 1 hit
    SUPFAMiSSF118215 SSF118215, 1 hit
    PROSITEiView protein in PROSITE
    PS00713 NA_DICARBOXYL_SYMP_1, 1 hit
    PS00714 NA_DICARBOXYL_SYMP_2, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLTP_ECOLI
    AccessioniPrimary (citable) accession number: P21345
    Secondary accession number(s): Q2M6M7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
    Last sequence update: March 1, 1992
    Last modified: October 10, 2018
    This is version 146 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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    Main funding by: National Institutes of Health

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