UniProtKB - P21338 (RNI_ECOLI)
Protein
Ribonuclease I
Gene
rna
Organism
Escherichia coli (strain K12)
Status
Functioni
One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid.
Catalytic activityi
- RNA containing adenosine-cytidine + H(2)O = an (RNA fragment)-3'-cytidine-3'-phosphate + a 5'-a hydroxy-adenosine -3'-(RNA fragment). EC:4.6.1.21
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 78 | By similarity | 1 | |
Active sitei | 152 | By similarity | 1 | |
Active sitei | 156 | By similarity | 1 |
GO - Molecular functioni
- Enterobacter ribonuclease activity Source: UniProtKB-EC
- lyase activity Source: UniProtKB-KW
- ribonuclease T2 activity Source: InterPro
- RNA binding Source: InterPro
GO - Biological processi
- RNA catabolic process Source: EcoCyc
Keywordsi
Molecular function | Endonuclease, Hydrolase, Lyase, Nuclease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10856-MONOMER MetaCyc:EG10856-MONOMER |
BRENDAi | 3.1.27.5, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease I (EC:4.6.1.21)Short name: RNase I Alternative name(s): Enterobacter ribonuclease |
Gene namesi | Name:rna Synonyms:rnsA Ordered Locus Names:b0611, JW0603 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
Cytoplasm, PeriplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 1 PublicationAdd BLAST | 23 | |
ChainiPRO_0000030961 | 24 – 268 | Ribonuclease IAdd BLAST | 245 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 103 ↔ 159 | By similarity |
Post-translational modificationi
Contains four disulfide bonds.By similarity
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P21338 |
PaxDbi | P21338 |
PRIDEi | P21338 |
Interactioni
Subunit structurei
Monomer.
Protein-protein interaction databases
BioGRIDi | 4259901, 35 interactors |
IntActi | P21338, 1 interactor |
STRINGi | 511145.b0611 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P21338 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P21338 |
Family & Domainsi
Sequence similaritiesi
Belongs to the RNase T2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG3719, Bacteria |
HOGENOMi | CLU_066430_1_0_6 |
InParanoidi | P21338 |
Family and domain databases
CDDi | cd01062, RNase_T2_prok, 1 hit |
Gene3Di | 3.90.730.10, 1 hit |
InterProi | View protein in InterPro IPR001568, RNase_T2-like IPR036430, RNase_T2-like_sf IPR018188, RNase_T2_His_AS_1 IPR033130, RNase_T2_His_AS_2 IPR039378, RNase_T2_prok |
PANTHERi | PTHR11240, PTHR11240, 1 hit |
Pfami | View protein in Pfam PF00445, Ribonuclease_T2, 1 hit |
SUPFAMi | SSF55895, SSF55895, 1 hit |
PROSITEi | View protein in PROSITE PS00530, RNASE_T2_1, 1 hit PS00531, RNASE_T2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P21338-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAFWRNAAL LAVSLLPFSS ANALALQAKQ YGDFDRYVLA LSWQTGFCQS
60 70 80 90 100
QHDRNRNERD ECRLQTETTN KADFLTVHGL WPGLPKSVAA RGVDERRWMR
110 120 130 140 150
FGCATRPIPN LPEARASRMC SSPETGLSLE TAAKLSEVMP GAGGRSCLER
160 170 180 190 200
YEYAKHGACF GFDPDAYFGT MVRLNQEIKE SEAGKFLADN YGKTVSRRDF
210 220 230 240 250
DAAFAKSWGK ENVKAVKLTC QGNPAYLTEI QISIKADAIN APLSANSFLP
260
QPHPGNCGKT FVIDKAGY
Sequence cautioni
The sequence AAB40811 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55687 Genomic DNA Translation: AAA24548.1 U82598 Genomic DNA Translation: AAB40811.1 Different initiation. U00096 Genomic DNA Translation: AAC73712.1 AP009048 Genomic DNA Translation: BAA35240.1 |
PIRi | JQ0777 |
RefSeqi | NP_415144.1, NC_000913.3 WP_000643397.1, NZ_SSZK01000032.1 |
Genome annotation databases
EnsemblBacteriai | AAC73712; AAC73712; b0611 BAA35240; BAA35240; BAA35240 |
GeneIDi | 949065 |
KEGGi | ecj:JW0603 eco:b0611 |
PATRICi | fig|1411691.4.peg.1657 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55687 Genomic DNA Translation: AAA24548.1 U82598 Genomic DNA Translation: AAB40811.1 Different initiation. U00096 Genomic DNA Translation: AAC73712.1 AP009048 Genomic DNA Translation: BAA35240.1 |
PIRi | JQ0777 |
RefSeqi | NP_415144.1, NC_000913.3 WP_000643397.1, NZ_SSZK01000032.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2EA1 | X-ray | 1.80 | A | 24-268 | [»] | |
2PQX | X-ray | 1.42 | A | 24-268 | [»] | |
2PQY | X-ray | 2.30 | A | 24-268 | [»] | |
2Z70 | X-ray | 1.70 | A | 24-268 | [»] | |
SMRi | P21338 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259901, 35 interactors |
IntActi | P21338, 1 interactor |
STRINGi | 511145.b0611 |
Proteomic databases
jPOSTi | P21338 |
PaxDbi | P21338 |
PRIDEi | P21338 |
Genome annotation databases
EnsemblBacteriai | AAC73712; AAC73712; b0611 BAA35240; BAA35240; BAA35240 |
GeneIDi | 949065 |
KEGGi | ecj:JW0603 eco:b0611 |
PATRICi | fig|1411691.4.peg.1657 |
Organism-specific databases
EchoBASEi | EB0849 |
Phylogenomic databases
eggNOGi | COG3719, Bacteria |
HOGENOMi | CLU_066430_1_0_6 |
InParanoidi | P21338 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10856-MONOMER MetaCyc:EG10856-MONOMER |
BRENDAi | 3.1.27.5, 2026 |
Miscellaneous databases
EvolutionaryTracei | P21338 |
PROi | PR:P21338 |
Family and domain databases
CDDi | cd01062, RNase_T2_prok, 1 hit |
Gene3Di | 3.90.730.10, 1 hit |
InterProi | View protein in InterPro IPR001568, RNase_T2-like IPR036430, RNase_T2-like_sf IPR018188, RNase_T2_His_AS_1 IPR033130, RNase_T2_His_AS_2 IPR039378, RNase_T2_prok |
PANTHERi | PTHR11240, PTHR11240, 1 hit |
Pfami | View protein in Pfam PF00445, Ribonuclease_T2, 1 hit |
SUPFAMi | SSF55895, SSF55895, 1 hit |
PROSITEi | View protein in PROSITE PS00530, RNASE_T2_1, 1 hit PS00531, RNASE_T2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RNI_ECOLI | |
Accessioni | P21338Primary (citable) accession number: P21338 Secondary accession number(s): P77101 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 165 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families