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Protein

Unconventional myosin-Vb

Gene

Myo5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis.By similarity

Caution

Was originally thought to be a glutamate decarboxylase (GAD).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi163 – 170ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Myosin
Biological processProtein transport, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-Vb
Gene namesi
Name:Myo5b
Synonyms:Kiaa1119
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106598 Myo5b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001234611 – 1818Unconventional myosin-VbAdd BLAST1818

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1416PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP21271
MaxQBiP21271
PaxDbiP21271
PRIDEiP21271

PTM databases

iPTMnetiP21271
PhosphoSitePlusiP21271

Expressioni

Gene expression databases

CleanExiMM_MYO5B

Interactioni

Subunit structurei

Component of the CART complex, at least composed of ACTN4, HGS/HRS, MYO5B and TRIM3. Interacts with RAB11FIP2 (By similarity). Interacts with RAB11A and RAB8A (By similarity). Found in a complex with CFTR and RAB11A (By similarity). Interacts with NPC1L1 (By similarity). Interacts with LIMA1 (PubMed:29880681).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiP21271, 2 interactors
MINTiP21271
STRINGi10090.ENSMUSP00000073790

Structurei

3D structure databases

ProteinModelPortaliP21271
SMRiP21271
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 60Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST53
Domaini69 – 762Myosin motorPROSITE-ProRule annotationAdd BLAST694
Domaini765 – 794IQ 1PROSITE-ProRule annotationAdd BLAST30
Domaini788 – 817IQ 2PROSITE-ProRule annotationAdd BLAST30
Domaini813 – 842IQ 3PROSITE-ProRule annotationAdd BLAST30
Domaini836 – 865IQ 4PROSITE-ProRule annotationAdd BLAST30
Domaini861 – 890IQ 5PROSITE-ProRule annotationAdd BLAST30
Domaini884 – 913IQ 6PROSITE-ProRule annotationAdd BLAST30
Domaini1496 – 1773DilutePROSITE-ProRule annotationAdd BLAST278

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 40Requires for interaction with LIMA1By similarityAdd BLAST20
Regioni641 – 663Actin-bindingSequence analysisAdd BLAST23

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1140 – 1261Sequence analysisAdd BLAST122
Coiled coili1313 – 1415Sequence analysisAdd BLAST103

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0160 Eukaryota
KOG0161 Eukaryota
COG5022 LUCA
HOGENOMiHOG000171839
HOVERGENiHBG052556
InParanoidiP21271
PhylomeDBiP21271

Family and domain databases

CDDicd15477 Myo5b_CBD, 1 hit
cd01380 MYSc_Myo5, 1 hit
Gene3Di2.30.30.360, 1 hit
3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR002710 Dilute_dom
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR037990 Myo5b_CBD
IPR001609 Myosin_head_motor_dom
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR036103 MYSc_Myo5
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01843 DIL, 1 hit
PF00612 IQ, 6 hits
PF00063 Myosin_head, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM01132 DIL, 1 hit
SM00015 IQ, 6 hits
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51126 DILUTE, 1 hit
PS50096 IQ, 6 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P21271-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSYSELYTRY TRVWIPDPDE VWRSAELTKD YKEGDKSLQL RLEDDTILEY
60 70 80 90 100
PVDVQNNQVP FLRNPDILVG ENDLTALSHL HEPAVLHNLK VRFLESNHIY
110 120 130 140 150
TYCGIVLVAI NPYEQLPIYG QDVIYAYSGQ NMGDMDPHIF AVAEEAYKQM
160 170 180 190 200
ARDEKNQSII VSGESGAGKT VSAKYAMRYF ATVGGSASDT NIEEKVLASS
210 220 230 240 250
PIMEAIGNAK TTRNDNSSRF GKFIEIGFDK KYHIIGANMR TYLLEKSRVV
260 270 280 290 300
FQADDERNYH IFYQLCAAAS LPEFKELALT CAEDFFYTAH GGNTTIEGVN
310 320 330 340 350
DADDFEKTRQ ALTLLGVRDS HQISIFKIIA SILHLGSVEI QSERDGDSCS
360 370 380 390 400
ISPQDEHLSN FCSLLGIEHS QMEHWLCHRK LVTTSETYVK TMSLQQVVNA
410 420 430 440 450
RDALAKHIYA QLFSWIVEHI NKALHTSHKQ HSFIGVLDIY GFETFEINSF
460 470 480 490 500
EQFCINYANE KLQQQFNSHV FKLEQEEYMK EQIPWTLIDF YDNQPCIDLI
510 520 530 540 550
EAKLGILDLL DEECKVPKGT DQNWAQKLYE RHSNSQHFQK PRMSNTAFIV
560 570 580 590 600
NHFADKVEYL SDGFLEKNRD TVYEEQINIL KASKFPLVAD LFHDDKDSAP
610 620 630 640 650
ATNTAKNRSS SKINVRSSRP LIKVPNKEHK KSVGYQFRTS LNLLMETLNA
660 670 680 690 700
TTPHYVRCIK PNDEKLPFHF DPKRAVQQLR ACGVLETIRI SAAGYPSRWT
710 720 730 740 750
YHDFFNRYRV LMKKRELTNT DKKNICKSVL ESLIKDPDKF QFGRTKIFFR
760 770 780 790 800
AGQVAYLEKL RADKFREATI MIQKSVRGWL QRVKYRRLRA ATLSLQRFCR
810 820 830 840 850
GYLARRLAEH LRRTRAAIVF QKQYRMLKAR RAYRRVCRAT VIIQSFTRAM
860 870 880 890 900
FVRRNYRQVL MEHKATIIQK YARGWMARKR FLRERDAAIV IQCAFRRLKA
910 920 930 940 950
RQELKALKIE ARSAEHLKRL NVGMENKVVQ LQRKIDDQNK EFKTLSEQLS
960 970 980 990 1000
AVTSSHAVEV EKLKKELAHY QQNQEADTSL QLQEEVQSLR TELQKAHSER
1010 1020 1030 1040 1050
RVLEDAHNKE NGELRKRVAD LEHENALLKD EKEYLNNQIL CQSKAESSQS
1060 1070 1080 1090 1100
SVEENLLMKK ELEEERSRYQ NLVKEYSQLE QRYENLRDEQ TPGHRKNPSN
1110 1120 1130 1140 1150
QSSLESDSNY PSISTSEIGD TEDALQQVEE IGIEKAAMDM TVFLKLQKRV
1160 1170 1180 1190 1200
RELEQERKKL QAQLEKGQQD SKKGQVEQQN NGLDVDQDAD IAYNSLKRQE
1210 1220 1230 1240 1250
LESENKKLKN DLNELRKAVA DQAMQDNSTH SSPDSYSLLL NQLKLANEEL
1260 1270 1280 1290 1300
EVRKEEVLIL RTQIMNADQR RLSGKNMEPN INARTSWPNS EKHVDQEDAI
1310 1320 1330 1340 1350
EAYHGVCQTN RLLEAQLQAQ SLEHEEEVEH LKAQVEALKE EMDKQQQTFC
1360 1370 1380 1390 1400
QTLLLSPEAQ VEFGVQQEIS RLTNENLDFK ELVEKLEKNE RKLKKQLKIY
1410 1420 1430 1440 1450
MKKVQDLEAA QALAQSDRRH HELTRQVTVQ RKEKDFQGML EYHKEDEALL
1460 1470 1480 1490 1500
IRNLVTDLKP QMLSGTVPCL PAYILYMCIR HADYTNDDLK VHSLLSSTIN
1510 1520 1530 1540 1550
GIKKVLKKHN DDFEMTSFWL SNTCRFLHCL KQYSGDEGFM TQNTAKQNEH
1560 1570 1580 1590 1600
CLKNFDLTEY RQVLSDLSIQ IYQQLIKIAE GLLQPMIVSA MLENESIQGL
1610 1620 1630 1640 1650
SGVRPTGYRK RSSSMVDGEN SYCLEAIVRQ MNSFHTVLCD QGLDPEIILQ
1660 1670 1680 1690 1700
VFKQLFYMIN AVTLNNLLLR KDACSWSTGM QLRYNISQLE EWLRGKNLHQ
1710 1720 1730 1740 1750
SGAVQTMEPL IQAAQLLQLK KKTHEDAEAI CSLCTSLSTQ QIVKILNLYT
1760 1770 1780 1790 1800
PLNEFEERVT VSFIRTIQAQ LQERNDPQQL LLDSKHVFPV LFPYNPSALT
1810
MDSIHIPACL NLEFLNEV
Length:1,818
Mass (Da):210,570
Last modified:January 9, 2007 - v2
Checksum:i78CFFA626CADC4A0
GO
Isoform 3 (identifier: P21271-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1310-1310: N → NSQTEDWGYLNEDGELGLAYQGLKQVA

Show »
Length:1,844
Mass (Da):213,437
Checksum:iECC7E517380C685E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G5E8G6G5E8G6_MOUSE
Myosin Vb, isoform CRA_c
Myo5b mCG_123286
1,818Annotation score:
G3X9Y9G3X9Y9_MOUSE
Myosin Vb, isoform CRA_a
Myo5b mCG_123286
1,844Annotation score:
D3Z135D3Z135_MOUSE
Unconventional myosin-Vb
Myo5b
183Annotation score:
H3BLK6H3BLK6_MOUSE
Unconventional myosin-Vb
Myo5b
128Annotation score:

Sequence cautioni

The sequence AAA37655 differs from that shown. Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti829A → V in AAH46444 (PubMed:15489334).Curated1
Sequence conflicti840T → A in AAH46444 (PubMed:15489334).Curated1
Sequence conflicti879 – 880KR → RC in AAH46444 (PubMed:15489334).Curated2
Sequence conflicti884E → Q in AAH46444 (PubMed:15489334).Curated1
Sequence conflicti1217 – 1218KA → NG in AAA37655 (PubMed:2236059).Curated2
Sequence conflicti1257V → A in AAA37655 (PubMed:2236059).Curated1
Sequence conflicti1446D → V in AAA37655 (PubMed:2236059).Curated1
Sequence conflicti1464S → L in AAA37655 (PubMed:2236059).Curated1
Sequence conflicti1544T → I in AAA37655 (PubMed:2236059).Curated1
Sequence conflicti1578 – 1579IA → MP in AAA37655 (PubMed:2236059).Curated2
Sequence conflicti1620 – 1631Missing in AAA37655 (PubMed:2236059).CuratedAdd BLAST12

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0220991310N → NSQTEDWGYLNEDGELGLAY QGLKQVA in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC011494 mRNA Translation: AAH11494.1
BC046444 mRNA Translation: AAH46444.1
BC118525 mRNA Translation: AAI18526.1
AK173102 Transcribed RNA Translation: BAD32380.1
M55253 mRNA Translation: AAA37655.1 Sequence problems.
CCDSiCCDS37858.1 [P21271-1]
PIRiA36481
UniGeneiMm.260098

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC011494 mRNA Translation: AAH11494.1
BC046444 mRNA Translation: AAH46444.1
BC118525 mRNA Translation: AAI18526.1
AK173102 Transcribed RNA Translation: BAD32380.1
M55253 mRNA Translation: AAA37655.1 Sequence problems.
CCDSiCCDS37858.1 [P21271-1]
PIRiA36481
UniGeneiMm.260098

3D structure databases

ProteinModelPortaliP21271
SMRiP21271
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP21271, 2 interactors
MINTiP21271
STRINGi10090.ENSMUSP00000073790

PTM databases

iPTMnetiP21271
PhosphoSitePlusiP21271

Proteomic databases

EPDiP21271
MaxQBiP21271
PaxDbiP21271
PRIDEiP21271

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:106598 Myo5b
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0160 Eukaryota
KOG0161 Eukaryota
COG5022 LUCA
HOGENOMiHOG000171839
HOVERGENiHBG052556
InParanoidiP21271
PhylomeDBiP21271

Miscellaneous databases

ChiTaRSiMyo5b mouse
PROiPR:P21271
SOURCEiSearch...

Gene expression databases

CleanExiMM_MYO5B

Family and domain databases

CDDicd15477 Myo5b_CBD, 1 hit
cd01380 MYSc_Myo5, 1 hit
Gene3Di2.30.30.360, 1 hit
3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR002710 Dilute_dom
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR037990 Myo5b_CBD
IPR001609 Myosin_head_motor_dom
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR036103 MYSc_Myo5
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF01843 DIL, 1 hit
PF00612 IQ, 6 hits
PF00063 Myosin_head, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM01132 DIL, 1 hit
SM00015 IQ, 6 hits
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51126 DILUTE, 1 hit
PS50096 IQ, 6 hits
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYO5B_MOUSE
AccessioniPrimary (citable) accession number: P21271
Secondary accession number(s): Q148A3
, Q69ZR6, Q811F6, Q91X59
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 9, 2007
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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