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Protein

Cyclin-dependent kinase inhibitor FAR1

Gene

FAR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitor of the cyclin-dependent kinase CDC28. Necessary for cell cycle arrest. Involved in pheromone response. Contributes to mating efficiency. Required for oriented polarization of yeast cells in response to mating pheromones.

Miscellaneous

Present with 238 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri202 – 252RING-typePROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • maintenance of protein location in nucleus Source: SGD
  • mitotic cell cycle G1 arrest in response to pheromone Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD

Keywordsi

Molecular functionProtein kinase inhibitor
Biological processCell cycle, Cell division
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31597-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase inhibitor FAR1
Short name:
CKI FAR1
Alternative name(s):
Factor arrest protein
Gene namesi
Name:FAR1
Ordered Locus Names:YJL157C
ORF Names:J0565
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL157C
SGDiS000003693 FAR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Some mutants appear to be defective in mating because they are unable to locate the mating partner.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87S → A: Prevents cell cycle-dependent degradation of FAR1. 1 Publication1
Mutagenesisi306T → A: Abolishes G1 arrest function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559101 – 830Cyclin-dependent kinase inhibitor FAR1Add BLAST830

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei87Phosphoserine; by CDC28Curated1
Modified residuei110PhosphoserineCombined sources1
Modified residuei114PhosphoserineCombined sources1
Modified residuei306PhosphothreonineCombined sources1

Post-translational modificationi

Thought to be phosphorylated by MAP kinase FUS3. Thought to enhance the binding of FAR1 to G1-specific cyclin-dependent kinase (CDK) complexes.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP21268
PaxDbiP21268
PRIDEiP21268

PTM databases

iPTMnetiP21268

Expressioni

Inductioni

By alpha-factor in a cells.

Interactioni

Subunit structurei

Associates with the CDC28-CLN complex.

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33603, 84 interactors
ComplexPortaliCPX-977 CDC24-FAR1-BG complex
DIPiDIP-2229N
IntActiP21268, 46 interactors
MINTiP21268
STRINGi4932.YJL157C

Structurei

3D structure databases

ProteinModelPortaliP21268
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

There is evidence to suggest that the N-terminal part may be sufficient for cell cycle arrest and the C-terminal may be necessary for some step in mating.

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri202 – 252RING-typePROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Zinc-finger

Phylogenomic databases

InParanoidiP21268
KOiK06652
OMAiCENISST
OrthoDBiEOG092C0N3E

Family and domain databases

InterProiView protein in InterPro
IPR001841 Znf_RING
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequencei

Sequence statusi: Complete.

P21268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTPTRVSFE KKIHTPPSGD RDAERSPPKK FLRGLSGKVF RKTPEFKKQQ
60 70 80 90 100
MPTFGYIEES QFTPNLGLMM SKRGNIPKPL NLSKPISPPP SLKKTAGSVA
110 120 130 140 150
SGFSKTGQLS ALQSPVNITS SNKYNIKATN LTTSLLRESI SDSTTMCDTL
160 170 180 190 200
SDINLTVMDE DYRIDGDSYY EEDSPTFMIS LERNIKKCNS QFSPKRYIGE
210 220 230 240 250
KCLICEESIS STFTGEKVVE STCSHTSHYN CYLMLFETLY FQGKFPECKI
260 270 280 290 300
CGEVSKPKDK DIVPEMVSKL LTGAGAHDDG PSSNMQQQWI DLKTARSFTG
310 320 330 340 350
EFPQFTPQEQ LIRTADISCD GFRTPRLSNS NQFEAVSYLD SPFLNSPFVN
360 370 380 390 400
KMATTDPFDL SDDEKLDCDD EIDESAAEVW FSKTGGEHVM VSVKFQEMRT
410 420 430 440 450
SDDLGVLQDV NHVDHEELEE REKEWKKKID QYIETNVDKD SEFGSLILFD
460 470 480 490 500
KLMYSDDGEQ WVDNNLVILF SKFLVLFDFE EMKILGKIPR DQFYQVIKFN
510 520 530 540 550
EDVLLCSLKS TNIPEIYLRF NENCEKWLLP KWKYCLENSS LETLPLSEIV
560 570 580 590 600
STVKELSHVN IIGALGAPPD VISAQSHDSR LPWKRLHSDT PLKLIVCLNL
610 620 630 640 650
SHADGELYRK RVLKSVHQIL DGLNTDDLLG IVVVGRDGSG VVGPFGTFIG
660 670 680 690 700
MINKNWDGWT TFLDNLEVVN PNVFRDEKQQ YKVTLQTCER LASTSAYVDT
710 720 730 740 750
DDHIATGYAK QILVLNGSDV VDIEHDQKLK KAFDQLSYHW RYEISQRRMT
760 770 780 790 800
PLNASIKQFL EELHTKRYLD VTLRLPQATF EQVYLGDMAA GEQKTRLIMD
810 820 830
EHPHSSLIEI EYFDLVKQQR IHQTLEVPNL
Length:830
Mass (Da):94,572
Last modified:November 1, 1995 - v2
Checksum:i834EBB2D7BB9641D
GO

Sequence cautioni

The sequence AAA34600 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti20D → N (PubMed:2147873).Curated1
Sequence conflicti568P → R in AAA34600 (PubMed:2147873).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti87S → P in FAR1-22P; induces cell cycle arrest in the absence of alpha-factor. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60071 Genomic DNA Translation: AAA34600.1 Different initiation.
Z49432 Genomic DNA Translation: CAA89452.1
BK006943 Genomic DNA Translation: DAA08646.1
PIRiS56940
RefSeqiNP_012378.1, NM_001181590.1

Genome annotation databases

EnsemblFungiiYJL157C; YJL157C; YJL157C
GeneIDi853283
KEGGisce:YJL157C

Similar proteinsi

Entry informationi

Entry nameiFAR1_YEAST
AccessioniPrimary (citable) accession number: P21268
Secondary accession number(s): D6VW30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: November 1, 1995
Last modified: July 18, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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