UniProtKB - P21264 (PUR6_YEAST)
Protein
Phosphoribosylaminoimidazole carboxylase
Gene
ADE2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
Present with 5410 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H+ = 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + CO2EC:4.1.1.21
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).Proteins known to be involved in this subpathway in this organism are:
- Phosphoribosylaminoimidazole carboxylase (ADE2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 138 – 193 | ATPPROSITE-ProRule annotationAdd BLAST | 56 |
GO - Molecular functioni
- 5-amino-4-imidazole carboxylate lyase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: InterPro
- phosphoribosylaminoimidazole carboxylase activity Source: SGD
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: SGD
- purine nucleobase metabolic process Source: SGD
- purine nucleotide biosynthetic process Source: SGD
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YOR128C-MONOMER |
BRENDAi | 4.1.1.21, 984 |
UniPathwayi | UPA00074;UER00130 |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)Alternative name(s): AIR carboxylase Short name: AIRC |
Gene namesi | Name:ADE2 Ordered Locus Names:YOR128C ORF Names:O3293, YOR3293C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000005654, ADE2 |
VEuPathDBi | FungiDB:YOR128C |
Subcellular locationi
Other locations
- cytoplasm Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000075028 | 1 – 571 | Phosphoribosylaminoimidazole carboxylaseAdd BLAST | 571 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 37 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P21264 |
PaxDbi | P21264 |
PRIDEi | P21264 |
TopDownProteomicsi | P21264 |
PTM databases
iPTMneti | P21264 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 34523, 95 interactors |
DIPi | DIP-1171N |
IntActi | P21264, 11 interactors |
MINTi | P21264 |
STRINGi | 4932.YOR128C |
Miscellaneous databases
RNActi | P21264, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 110 – 298 | ATP-graspPROSITE-ProRule annotationAdd BLAST | 189 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2835, Eukaryota |
HOGENOMi | CLU_011534_2_1_1 |
InParanoidi | P21264 |
OMAi | ITFDHEH |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit 3.40.50.7700, 1 hit |
HAMAPi | MF_01929, PurE_classI, 1 hit MF_01928, PurK, 1 hit |
InterProi | View protein in InterPro IPR016301, Ade2_fungi/plant IPR011761, ATP-grasp IPR003135, ATP-grasp_carboxylate-amine IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR033747, PurE_ClassI IPR000031, PurE_dom IPR035893, PurE_sf IPR005875, PurK IPR040686, PurK_C IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF00731, AIRC, 1 hit PF02222, ATP-grasp, 1 hit PF17769, PurK_C, 1 hit |
PIRSFi | PIRSF001340, AIR_carboxylase, 1 hit |
SMARTi | View protein in SMART SM01001, AIRC, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52255, SSF52255, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01162, purE, 1 hit TIGR01161, purK, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
i Sequence
Sequence statusi: Complete.
P21264-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSRTVGILG GGQLGRMIVE AANRLNIKTV ILDAENSPAK QISNSNDHVN
60 70 80 90 100
GSFSNPLDIE KLAEKCDVLT IEIEHVDVPT LKNLQVKHPK LKIYPSPETI
110 120 130 140 150
RLIQDKYIQK EHLIKNGIAV TQSVPVEQAS ETSLLNVGRD LGFPFVLKSR
160 170 180 190 200
TLAYDGRGNF VVKNKEMIPE ALEVLKDRPL YAEKWAPFTK ELAVMIVRSV
210 220 230 240 250
NGLVFSYPIV ETIHKDNICD LCYAPARVPD SVQLKAKLLA ENAIKSFPGC
260 270 280 290 300
GIFGVEMFYL ETGELLINEI APRPHNSGHY TIDACVTSQF EAHLRSILDL
310 320 330 340 350
PMPKNFTSFS TITTNAIMLN VLGDKHTKDK ELETCERALA TPGSSVYLYG
360 370 380 390 400
KESRPNRKVG HINIIASSMA ECEQRLNYIT GRTDIPIKIS VAQKLDLEAM
410 420 430 440 450
VKPLVGIIMG SDSDLPVMSA ACAVLKDFGV PFEVTIVSAH RTPHRMSAYA
460 470 480 490 500
ISASKRGIKT IIAGAGGAAH LPGMVAAMTP LPVIGVPVKG SCLDGVDSLH
510 520 530 540 550
SIVQMPRGVP VATVAINNST NAALLAVRLL GAYDSSYTTK MEQFLLKQEE
560 570
EVLVKAQKLE TVGYEAYLEN K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 101 | R → G in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 186 | A → G in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 206 | S → F in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 241 – 253 | ENAIK…GCGIF → KMQSNFSRLWYI (PubMed:1514324).CuratedAdd BLAST | 13 | |
Sequence conflicti | 387 | I → L in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 407 | I → V in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 431 | P → T in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 434 | V → L in AAA34407 (PubMed:1514324).Curated | 1 | |
Sequence conflicti | 502 | I → T in AAA34407 (PubMed:1514324).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59824 Genomic DNA Translation: AAA34401.1 M58324 Genomic DNA Translation: AAA34407.1 X90518 Genomic DNA Translation: CAA62125.1 X94335 Genomic DNA Translation: CAA64047.1 Z75036 Genomic DNA Translation: CAA99327.1 BK006948 Genomic DNA Translation: DAA10902.1 |
PIRi | JN0098, DEBYP |
RefSeqi | NP_014771.3, NM_001183547.3 |
Genome annotation databases
EnsemblFungii | YOR128C_mRNA; YOR128C; YOR128C |
GeneIDi | 854295 |
KEGGi | sce:YOR128C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59824 Genomic DNA Translation: AAA34401.1 M58324 Genomic DNA Translation: AAA34407.1 X90518 Genomic DNA Translation: CAA62125.1 X94335 Genomic DNA Translation: CAA64047.1 Z75036 Genomic DNA Translation: CAA99327.1 BK006948 Genomic DNA Translation: DAA10902.1 |
PIRi | JN0098, DEBYP |
RefSeqi | NP_014771.3, NM_001183547.3 |
3D structure databases
SMRi | P21264 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 34523, 95 interactors |
DIPi | DIP-1171N |
IntActi | P21264, 11 interactors |
MINTi | P21264 |
STRINGi | 4932.YOR128C |
PTM databases
iPTMneti | P21264 |
Proteomic databases
MaxQBi | P21264 |
PaxDbi | P21264 |
PRIDEi | P21264 |
TopDownProteomicsi | P21264 |
Genome annotation databases
EnsemblFungii | YOR128C_mRNA; YOR128C; YOR128C |
GeneIDi | 854295 |
KEGGi | sce:YOR128C |
Organism-specific databases
SGDi | S000005654, ADE2 |
VEuPathDBi | FungiDB:YOR128C |
Phylogenomic databases
eggNOGi | KOG2835, Eukaryota |
HOGENOMi | CLU_011534_2_1_1 |
InParanoidi | P21264 |
OMAi | ITFDHEH |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00130 |
BioCyci | MetaCyc:YOR128C-MONOMER |
BRENDAi | 4.1.1.21, 984 |
Miscellaneous databases
PHI-basei | PHI:501 |
PROi | PR:P21264 |
RNActi | P21264, protein |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit 3.40.50.7700, 1 hit |
HAMAPi | MF_01929, PurE_classI, 1 hit MF_01928, PurK, 1 hit |
InterProi | View protein in InterPro IPR016301, Ade2_fungi/plant IPR011761, ATP-grasp IPR003135, ATP-grasp_carboxylate-amine IPR013815, ATP_grasp_subdomain_1 IPR016185, PreATP-grasp_dom_sf IPR033747, PurE_ClassI IPR000031, PurE_dom IPR035893, PurE_sf IPR005875, PurK IPR040686, PurK_C IPR011054, Rudment_hybrid_motif |
Pfami | View protein in Pfam PF00731, AIRC, 1 hit PF02222, ATP-grasp, 1 hit PF17769, PurK_C, 1 hit |
PIRSFi | PIRSF001340, AIR_carboxylase, 1 hit |
SMARTi | View protein in SMART SM01001, AIRC, 1 hit |
SUPFAMi | SSF51246, SSF51246, 1 hit SSF52255, SSF52255, 1 hit SSF52440, SSF52440, 1 hit |
TIGRFAMsi | TIGR01162, purE, 1 hit TIGR01161, purK, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR6_YEAST | |
Accessioni | P21264Primary (citable) accession number: P21264 Secondary accession number(s): D6W2I6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 1991 | |
Last modified: | February 10, 2021 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XV
Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families