UniProtKB - P21263 (NEST_RAT)
Nestin
Nes
Functioni
Required for brain and eye development (By similarity).
Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
By similarity2 PublicationsGO - Molecular functioni
- CCR5 chemokine receptor binding Source: RGD
- intermediate filament binding Source: UniProtKB
GO - Biological processi
- brain development Source: UniProtKB
- cell projection morphogenesis Source: RGD
- embryonic camera-type eye development Source: UniProtKB
- G2/M transition of mitotic cell cycle Source: RGD
- negative regulation of catalytic activity Source: RGD
- negative regulation of neuron apoptotic process Source: RGD
- negative regulation of protein binding Source: RGD
- nervous system development Source: RGD
- neuron differentiation Source: RGD
- positive regulation of intermediate filament depolymerization Source: UniProtKB
- positive regulation of neural precursor cell proliferation Source: UniProtKB
- response to ionizing radiation Source: RGD
- response to nutrient levels Source: RGD
- response to xenobiotic stimulus Source: RGD
- stem cell proliferation Source: RGD
Keywordsi
Molecular function | Developmental protein |
Biological process | Neurogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Nestin |
Gene namesi | Name:Nes |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3162, Nes |
Subcellular locationi
Cytoskeleton
- intermediate filament Source: RGD
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
Intermediate filamentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063854 | 1 – 1893 | NestinAdd BLAST | 1893 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 312 | PhosphoserineBy similarity | 1 | |
Modified residuei | 316 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 356 | PhosphoserineBy similarity | 1 | |
Modified residuei | 359 | PhosphoserineBy similarity | 1 | |
Modified residuei | 389 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 562 | PhosphoserineBy similarity | 1 | |
Modified residuei | 620 | PhosphoserineCombined sources | 1 | |
Modified residuei | 685 | PhosphoserineBy similarity | 1 | |
Modified residuei | 729 | PhosphoserineCombined sources | 1 | |
Modified residuei | 817 | PhosphoserineCombined sources | 1 | |
Modified residuei | 903 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1005 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1049 | PhosphoserineCombined sources | 1 | |
Cross-linki | 1136 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 1136 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 1145 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1166 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1216 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1229 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1322 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1570 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1594 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1686 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1695 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1772 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1774 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1866 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1889 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1890 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P21263 |
PaxDbi | P21263 |
PRIDEi | P21263 |
PTM databases
iPTMneti | P21263 |
PhosphoSitePlusi | P21263 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25% (By similarity).
Interacts with FHOD3.
By similarity1 PublicationGO - Molecular functioni
- CCR5 chemokine receptor binding Source: RGD
Protein-protein interaction databases
IntActi | P21263, 5 interactors |
MINTi | P21263 |
STRINGi | 10116.ENSRNOP00000025314 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 314 | IF rodPROSITE-ProRule annotationAdd BLAST | 307 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 7 | Head | 7 | |
Regioni | 8 – 43 | Coil 1AAdd BLAST | 36 | |
Regioni | 44 – 55 | Linker 1Add BLAST | 12 | |
Regioni | 56 – 151 | Coil 1BAdd BLAST | 96 | |
Regioni | 150 – 172 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 152 – 174 | Linker 12Add BLAST | 23 | |
Regioni | 175 – 193 | Coil 2AAdd BLAST | 19 | |
Regioni | 194 – 196 | Linker 2 | 3 | |
Regioni | 197 – 314 | Coil 2BAdd BLAST | 118 | |
Regioni | 315 – 1893 | TailAdd BLAST | 1579 | |
Regioni | 437 – 479 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 507 – 529 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 556 – 879 | DisorderedSequence analysisAdd BLAST | 324 | |
Regioni | 949 – 1130 | DisorderedSequence analysisAdd BLAST | 182 | |
Regioni | 1155 – 1222 | DisorderedSequence analysisAdd BLAST | 68 | |
Regioni | 1237 – 1263 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 1336 – 1369 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 1388 – 1824 | DisorderedSequence analysisAdd BLAST | 437 | |
Regioni | 1870 – 1893 | DisorderedSequence analysisAdd BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 569 – 593 | Basic and acidic residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 607 – 621 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 670 – 706 | Basic and acidic residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 713 – 740 | Basic and acidic residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 757 – 879 | Basic and acidic residuesSequence analysisAdd BLAST | 123 | |
Compositional biasi | 980 – 1060 | Basic and acidic residuesSequence analysisAdd BLAST | 81 | |
Compositional biasi | 1388 – 1404 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 1473 – 1491 | Basic and acidic residuesSequence analysisAdd BLAST | 19 | |
Compositional biasi | 1562 – 1576 | Acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1631 – 1645 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 1686 – 1709 | Acidic residuesSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | ENOG502RYFK, Eukaryota |
InParanoidi | P21263 |
PhylomeDBi | P21263 |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR031211, Nestin |
PANTHERi | PTHR47051, PTHR47051, 3 hits |
Pfami | View protein in Pfam PF00038, Filament, 2 hits |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDT
60 70 80 90 100
SWRARADDEL ASLRILVDQR WREKLEAEVQ RDNLAEELES VAGRCQQVRL
110 120 130 140 150
ARERTVQEAA CSRRALEAEK NARGWLSTQA AELERELEAL RAAHEEERAH
160 170 180 190 200
LNAQAACAPR RPPAPPHGSP VRAPEVEDLA RRLGEVWRGA VRDYQERVAH
210 220 230 240 250
MESSLGQARE RLSQAVRGAR ECRLEVQQLQ ADRDSLQERR EALEQRLEGR
260 270 280 290 300
WQDRLQATDK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV
310 320 330 340 350
ATYRTLLEAE NSRLQTPGRG SQASLGFLDP KLKPNFLGIP EDQYLGSVLP
360 370 380 390 400
ALSPTSFPSP LPNTLETPVT AFLKTQEFLQ ARTPTLASTP IPPISEAPCP
410 420 430 440 450
PNAEVRAQEV PLSLLQTQAP EPLWAEATVP SSSAILPELE EPGGKQQGHF
460 470 480 490 500
PDDLTSLATT LNPHHPTLEA KDGESSGSRV SSIFQEDEGQ IWELVEKEAD
510 520 530 540 550
IEVKVENSSA QKTQESGLDT EETQDSQGPL QKETLKALGE EPLMSLKIQN
560 570 580 590 600
YETAGKENCN SSTEGHLGTL EGPEKEKQIP LKSLEEKNVE SEKTLENGVP
610 620 630 640 650
VLSELLGKED TRTEDQELMS PKGTLKRFSS LGKESQEVVR PSKEGNLESW
660 670 680 690 700
TAFKEESQHP LGFPGAEDQM LERLVEKEDQ SFPRSPEEED QEACRPLQKE
710 720 730 740 750
NQEPLGYEEA EGQILERLIE KESQESLRSP EEEDQEAGRS LQKGNQEPLG
760 770 780 790 800
YEEAEGQILE RLIEKESQES LRSAEEEDQE ACRSLQKENQ EPLGYEEAED
810 820 830 840 850
QILERLIEKE SQESLRSPEE EDQEAGRSLQ KENQEPLGYE EAEDQMLERL
860 870 880 890 900
IEKESQESLK SPEENQRIGK PLERENQKSL RYLEENQETF VPLESRNQRP
910 920 930 940 950
LRSLEVEEEE QRIVKPLEKV SQDSLGSLAE ENVQPLRYLE EDNCINKSLL
960 970 980 990 1000
EDKTHKSLGS LEDRNGDSII IPQESETQVS LRPPEEEDQR IVNHLEKESQ
1010 1020 1030 1040 1050
EFSRSSEEEE RVMERSLEGE NHESLSSVEK EDQMVESQLE KESQDSGKSL
1060 1070 1080 1090 1100
EDESQETFGP LEKENAESLR SLAGQDQEEQ KLEQETQQTL RAVGNEQMAV
1110 1120 1130 1140 1150
SPPEKVDPEL PKPLGNDQEI ARSLGKENQE SLVSLKEKGI ETVKSLETEI
1160 1170 1180 1190 1200
IEPLETAEED LERRKSIDTQ EPLWSTEVAR ETVEPPEDEP PGSLGSVDEN
1210 1220 1230 1240 1250
RETLTSLEKE SQELSSLGKW NVETRVEDSQ QCLQVEEGLQ EEQHQESLRE
1260 1270 1280 1290 1300
VKQELPSSGN QQRWEDVVEG KAVGQEAPLA TTGVGTEDKA ELHLRGQGGE
1310 1320 1330 1340 1350
EEAAAEGELL QDIVGEAWSL GSSEPKEQRV PAEALDNLEG GALEVPVAQS
1360 1370 1380 1390 1400
MPEVTERDED RAQAGEQDSI EVTLGLEAAR TGLELEQEVV GLEDPRHFAR
1410 1420 1430 1440 1450
EEAIPPSLGE ESVKAKIAQG LEGPGKEPKE AGALDSGILE LPKTSSEALE
1460 1470 1480 1490 1500
CQGHEESESM EGWEEEEASL ETSDHEGSDA PQPRPPETEE DEGAQAALTA
1510 1520 1530 1540 1550
PGPKLLEPCS PIPILTDAHE LQPQAEGIQE AGWQPEAGSE ALERVENEPE
1560 1570 1580 1590 1600
FGLGEIPEGL QDWEEGREES EADDLGETLP DSTPLGLYLR SPASPKWDLA
1610 1620 1630 1640 1650
GEQRLSPQGD AGKEDWGPAV PAAQGLSGPP EEEEEQGHGS DLSSEEFEDL
1660 1670 1680 1690 1700
GTEASLLPGV PKEVADHVGQ VPPVLQPACW DQGGESDGFA DEEESGEEGE
1710 1720 1730 1740 1750
EEDADEEGAE SGAQWWGSGA SGGGCKVQDI AQRGDPVQES VGVSGLWDDG
1760 1770 1780 1790 1800
LRGAAANVPA LEMVSQDSAE PSGSEESESA SLEGEEGQVT DHLDAPQEVT
1810 1820 1830 1840 1850
SMVPGVGDAF DIGGQSPNLD SEQVNGKMEN GLEQAEGQVV LDGDEDQELL
1860 1870 1880 1890
LQGQEVGALK VPLVASPVHL GPSQPLKFTL SGVDGDSWSS GED
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3V8F8 | G3V8F8_RAT | Nestin | Nes rCG_62670 | 1,893 | Annotation score: | ||
D3ZWJ2 | D3ZWJ2_RAT | Nestin | Nes | 1,502 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 168 – 172 | GSPVR → RIPGP in AAA41685 (PubMed:1689217).Curated | 5 | |
Sequence conflicti | 425 – 426 | AE → LK in AAA41685 (PubMed:1689217).Curated | 2 | |
Sequence conflicti | 460 | T → N in AAA41685 (PubMed:1689217).Curated | 1 | |
Sequence conflicti | 477 | G → E in AAA41685 (PubMed:1689217).Curated | 1 | |
Sequence conflicti | 943 | N → D in AAA41685 (PubMed:1689217).Curated | 1 | |
Sequence conflicti | 1011 | R → Q in AAA41685 (PubMed:1689217).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_024924 | 736 – 823 | Missing in isoform 2. 1 PublicationAdd BLAST | 88 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34384 mRNA Translation: AAA41685.1 AF538924 mRNA Translation: AAN33053.1 |
PIRi | A34736 |
Genome annotation databases
UCSCi | RGD:3162, rat [P21263-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34384 mRNA Translation: AAA41685.1 AF538924 mRNA Translation: AAN33053.1 |
PIRi | A34736 |
3D structure databases
SMRi | P21263 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P21263, 5 interactors |
MINTi | P21263 |
STRINGi | 10116.ENSRNOP00000025314 |
PTM databases
iPTMneti | P21263 |
PhosphoSitePlusi | P21263 |
Proteomic databases
jPOSTi | P21263 |
PaxDbi | P21263 |
PRIDEi | P21263 |
Genome annotation databases
UCSCi | RGD:3162, rat [P21263-1] |
Organism-specific databases
RGDi | 3162, Nes |
Phylogenomic databases
eggNOGi | ENOG502RYFK, Eukaryota |
InParanoidi | P21263 |
PhylomeDBi | P21263 |
Miscellaneous databases
PROi | PR:P21263 |
Family and domain databases
InterProi | View protein in InterPro IPR018039, IF_conserved IPR039008, IF_rod_dom IPR031211, Nestin |
PANTHERi | PTHR47051, PTHR47051, 3 hits |
Pfami | View protein in Pfam PF00038, Filament, 2 hits |
SMARTi | View protein in SMART SM01391, Filament, 1 hit |
PROSITEi | View protein in PROSITE PS00226, IF_ROD_1, 1 hit PS51842, IF_ROD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NEST_RAT | |
Accessioni | P21263Primary (citable) accession number: P21263 Secondary accession number(s): Q8CJ14 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | May 1, 2007 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families