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UniProtKB - P21263 (NEST_RAT)

Entry version 130 (23 Feb 2022)
Sequence version 2 (01 May 2007)
Previous versions | rss
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Protein

Nestin

Gene

Nes

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for brain and eye development (By similarity).

Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

By similarity2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDevelopmental protein
Biological processNeurogenesis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nestin
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Nes
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unplaced

Organism-specific databases

Rat genome database

More...RGDi		3162, Nes

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Intermediate filament

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000638541 – 1893NestinAdd BLAST1893

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei1N-acetylmethionineBy similarity1
Modified residuei312PhosphoserineBy similarity1
Modified residuei316Phosphothreonine1 Publication1
Modified residuei356PhosphoserineBy similarity1
Modified residuei359PhosphoserineBy similarity1
Modified residuei389PhosphothreonineBy similarity1
Modified residuei562PhosphoserineBy similarity1
Modified residuei620PhosphoserineCombined sources1
Modified residuei685PhosphoserineBy similarity1
Modified residuei729PhosphoserineCombined sources1
Modified residuei817PhosphoserineCombined sources1
Modified residuei903PhosphoserineBy similarity1
Modified residuei1005PhosphoserineBy similarity1
Modified residuei1049PhosphoserineCombined sources1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki1136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki1136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei1145PhosphoserineBy similarity1
Modified residuei1166PhosphoserineCombined sources1
Modified residuei1216PhosphoserineBy similarity1
Modified residuei1229PhosphoserineBy similarity1
Modified residuei1322PhosphoserineBy similarity1
Modified residuei1570PhosphoserineBy similarity1
Modified residuei1594PhosphoserineCombined sources1
Modified residuei1686PhosphoserineBy similarity1
Modified residuei1695PhosphoserineBy similarity1
Modified residuei1772PhosphoserineBy similarity1
Modified residuei1774PhosphoserineBy similarity1
Modified residuei1866PhosphoserineCombined sources1
Modified residuei1889PhosphoserineCombined sources1
Modified residuei1890PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P21263

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P21263

PRoteomics IDEntifications database

More...PRIDEi		P21263

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P21263

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P21263

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

CNS stem cells.

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25% (By similarity).

Interacts with FHOD3.

By similarity1 Publication

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

Protein interaction database and analysis system

More...IntActi		P21263, 5 interactors

Molecular INTeraction database

More...MINTi		P21263

STRING: functional protein association networks

More...STRINGi		10116.ENSRNOP00000025314

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P21263

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini8 – 314IF rodPROSITE-ProRule annotationAdd BLAST307

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 7Head7
Regioni8 – 43Coil 1AAdd BLAST36
Regioni44 – 55Linker 1Add BLAST12
Regioni56 – 151Coil 1BAdd BLAST96
Regioni150 – 172DisorderedSequence analysisAdd BLAST23
Regioni152 – 174Linker 12Add BLAST23
Regioni175 – 193Coil 2AAdd BLAST19
Regioni194 – 196Linker 23
Regioni197 – 314Coil 2BAdd BLAST118
Regioni315 – 1893TailAdd BLAST1579
Regioni437 – 479DisorderedSequence analysisAdd BLAST43
Regioni507 – 529DisorderedSequence analysisAdd BLAST23
Regioni556 – 879DisorderedSequence analysisAdd BLAST324
Regioni949 – 1130DisorderedSequence analysisAdd BLAST182
Regioni1155 – 1222DisorderedSequence analysisAdd BLAST68
Regioni1237 – 1263DisorderedSequence analysisAdd BLAST27
Regioni1336 – 1369DisorderedSequence analysisAdd BLAST34
Regioni1388 – 1824DisorderedSequence analysisAdd BLAST437
Regioni1870 – 1893DisorderedSequence analysisAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi569 – 593Basic and acidic residuesSequence analysisAdd BLAST25
Compositional biasi607 – 621Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi670 – 706Basic and acidic residuesSequence analysisAdd BLAST37
Compositional biasi713 – 740Basic and acidic residuesSequence analysisAdd BLAST28
Compositional biasi757 – 879Basic and acidic residuesSequence analysisAdd BLAST123
Compositional biasi980 – 1060Basic and acidic residuesSequence analysisAdd BLAST81
Compositional biasi1388 – 1404Basic and acidic residuesSequence analysisAdd BLAST17
Compositional biasi1473 – 1491Basic and acidic residuesSequence analysisAdd BLAST19
Compositional biasi1562 – 1576Acidic residuesSequence analysisAdd BLAST15
Compositional biasi1631 – 1645Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi1686 – 1709Acidic residuesSequence analysisAdd BLAST24

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the intermediate filament family.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG502RYFK, Eukaryota

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P21263

Database for complete collections of gene phylogenies

More...PhylomeDBi		P21263

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR018039, IF_conserved
IPR039008, IF_rod_dom
IPR031211, Nestin

The PANTHER Classification System

More...PANTHERi		PTHR47051, PTHR47051, 3 hits

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00038, Filament, 2 hits

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM01391, Filament, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00226, IF_ROD_1, 1 hit
PS51842, IF_ROD_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P21263-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGCVGEESF QMWELNRRLE AYLTRVKTLE EQNQLLSAEL GGLRAQSGDT 
        60         70         80         90        100
SWRARADDEL ASLRILVDQR WREKLEAEVQ RDNLAEELES VAGRCQQVRL 
       110        120        130        140        150
ARERTVQEAA CSRRALEAEK NARGWLSTQA AELERELEAL RAAHEEERAH 
       160        170        180        190        200
LNAQAACAPR RPPAPPHGSP VRAPEVEDLA RRLGEVWRGA VRDYQERVAH 
       210        220        230        240        250
MESSLGQARE RLSQAVRGAR ECRLEVQQLQ ADRDSLQERR EALEQRLEGR 
       260        270        280        290        300
WQDRLQATDK FQLAVEALEQ EKQGLQSQIA QILEGGQQLA HLKMSLSLEV 
       310        320        330        340        350
ATYRTLLEAE NSRLQTPGRG SQASLGFLDP KLKPNFLGIP EDQYLGSVLP 
       360        370        380        390        400
ALSPTSFPSP LPNTLETPVT AFLKTQEFLQ ARTPTLASTP IPPISEAPCP 
       410        420        430        440        450
PNAEVRAQEV PLSLLQTQAP EPLWAEATVP SSSAILPELE EPGGKQQGHF 
       460        470        480        490        500
PDDLTSLATT LNPHHPTLEA KDGESSGSRV SSIFQEDEGQ IWELVEKEAD 
       510        520        530        540        550
IEVKVENSSA QKTQESGLDT EETQDSQGPL QKETLKALGE EPLMSLKIQN 
       560        570        580        590        600
YETAGKENCN SSTEGHLGTL EGPEKEKQIP LKSLEEKNVE SEKTLENGVP 
       610        620        630        640        650
VLSELLGKED TRTEDQELMS PKGTLKRFSS LGKESQEVVR PSKEGNLESW 
       660        670        680        690        700
TAFKEESQHP LGFPGAEDQM LERLVEKEDQ SFPRSPEEED QEACRPLQKE 
       710        720        730        740        750
NQEPLGYEEA EGQILERLIE KESQESLRSP EEEDQEAGRS LQKGNQEPLG 
       760        770        780        790        800
YEEAEGQILE RLIEKESQES LRSAEEEDQE ACRSLQKENQ EPLGYEEAED 
       810        820        830        840        850
QILERLIEKE SQESLRSPEE EDQEAGRSLQ KENQEPLGYE EAEDQMLERL 
       860        870        880        890        900
IEKESQESLK SPEENQRIGK PLERENQKSL RYLEENQETF VPLESRNQRP 
       910        920        930        940        950
LRSLEVEEEE QRIVKPLEKV SQDSLGSLAE ENVQPLRYLE EDNCINKSLL 
       960        970        980        990       1000
EDKTHKSLGS LEDRNGDSII IPQESETQVS LRPPEEEDQR IVNHLEKESQ 
      1010       1020       1030       1040       1050
EFSRSSEEEE RVMERSLEGE NHESLSSVEK EDQMVESQLE KESQDSGKSL 
      1060       1070       1080       1090       1100
EDESQETFGP LEKENAESLR SLAGQDQEEQ KLEQETQQTL RAVGNEQMAV 
      1110       1120       1130       1140       1150
SPPEKVDPEL PKPLGNDQEI ARSLGKENQE SLVSLKEKGI ETVKSLETEI 
      1160       1170       1180       1190       1200
IEPLETAEED LERRKSIDTQ EPLWSTEVAR ETVEPPEDEP PGSLGSVDEN 
      1210       1220       1230       1240       1250
RETLTSLEKE SQELSSLGKW NVETRVEDSQ QCLQVEEGLQ EEQHQESLRE 
      1260       1270       1280       1290       1300
VKQELPSSGN QQRWEDVVEG KAVGQEAPLA TTGVGTEDKA ELHLRGQGGE 
      1310       1320       1330       1340       1350
EEAAAEGELL QDIVGEAWSL GSSEPKEQRV PAEALDNLEG GALEVPVAQS 
      1360       1370       1380       1390       1400
MPEVTERDED RAQAGEQDSI EVTLGLEAAR TGLELEQEVV GLEDPRHFAR 
      1410       1420       1430       1440       1450
EEAIPPSLGE ESVKAKIAQG LEGPGKEPKE AGALDSGILE LPKTSSEALE 
      1460       1470       1480       1490       1500
CQGHEESESM EGWEEEEASL ETSDHEGSDA PQPRPPETEE DEGAQAALTA 
      1510       1520       1530       1540       1550
PGPKLLEPCS PIPILTDAHE LQPQAEGIQE AGWQPEAGSE ALERVENEPE 
      1560       1570       1580       1590       1600
FGLGEIPEGL QDWEEGREES EADDLGETLP DSTPLGLYLR SPASPKWDLA 
      1610       1620       1630       1640       1650
GEQRLSPQGD AGKEDWGPAV PAAQGLSGPP EEEEEQGHGS DLSSEEFEDL 
      1660       1670       1680       1690       1700
GTEASLLPGV PKEVADHVGQ VPPVLQPACW DQGGESDGFA DEEESGEEGE 
      1710       1720       1730       1740       1750
EEDADEEGAE SGAQWWGSGA SGGGCKVQDI AQRGDPVQES VGVSGLWDDG 
      1760       1770       1780       1790       1800
LRGAAANVPA LEMVSQDSAE PSGSEESESA SLEGEEGQVT DHLDAPQEVT 
      1810       1820       1830       1840       1850
SMVPGVGDAF DIGGQSPNLD SEQVNGKMEN GLEQAEGQVV LDGDEDQELL 
      1860       1870       1880       1890 
LQGQEVGALK VPLVASPVHL GPSQPLKFTL SGVDGDSWSS GED
Length:1,893
Mass (Da):208,797
Last modified:May 1, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i03AE6B616A1A7623
GO
Isoform 2 (identifier: P21263-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     736-823: Missing.

Show »
Length:1,805
Mass (Da):198,623
Checksum:iDC2A89784654D647
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V8F8G3V8F8_RAT
Nestin
Nes rCG_62670
1,893Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
D3ZWJ2D3ZWJ2_RAT
Nestin
Nes
1,502Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti168 – 172GSPVR → RIPGP in AAA41685 (PubMed:1689217).Curated5
Sequence conflicti425 – 426AE → LK in AAA41685 (PubMed:1689217).Curated2
Sequence conflicti460T → N in AAA41685 (PubMed:1689217).Curated1
Sequence conflicti477G → E in AAA41685 (PubMed:1689217).Curated1
Sequence conflicti943N → D in AAA41685 (PubMed:1689217).Curated1
Sequence conflicti1011R → Q in AAA41685 (PubMed:1689217).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_024924736 – 823Missing in isoform 2. 1 PublicationAdd BLAST88

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M34384 mRNA Translation: AAA41685.1
AF538924 mRNA Translation: AAN33053.1

Protein sequence database of the Protein Information Resource

More...PIRi		A34736

Genome annotation databases

UCSC genome browser

More...UCSCi		RGD:3162, rat [P21263-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34384 mRNA Translation: AAA41685.1
AF538924 mRNA Translation: AAN33053.1
PIRiA34736

3D structure databases

SMRiP21263
ModBaseiSearch...

Protein-protein interaction databases

IntActiP21263, 5 interactors
MINTiP21263
STRINGi10116.ENSRNOP00000025314

PTM databases

iPTMnetiP21263
PhosphoSitePlusiP21263

Proteomic databases

jPOSTiP21263
PaxDbiP21263
PRIDEiP21263

Genome annotation databases

UCSCiRGD:3162, rat [P21263-1]

Organism-specific databases

RGDi3162, Nes

Phylogenomic databases

eggNOGiENOG502RYFK, Eukaryota
InParanoidiP21263
PhylomeDBiP21263

Miscellaneous databases

Protein Ontology

More...PROi		PR:P21263

Family and domain databases

InterProiView protein in InterPro
IPR018039, IF_conserved
IPR039008, IF_rod_dom
IPR031211, Nestin
PANTHERiPTHR47051, PTHR47051, 3 hits
PfamiView protein in Pfam
PF00038, Filament, 2 hits
SMARTiView protein in SMART
SM01391, Filament, 1 hit
PROSITEiView protein in PROSITE
PS00226, IF_ROD_1, 1 hit
PS51842, IF_ROD_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNEST_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P21263
Secondary accession number(s): Q8CJ14
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: May 1, 2007
Last modified: February 23, 2022
This is version 130 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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