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Protein

Chaperonin 60 subunit beta 1, chloroplastic

Gene

CPN60B1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds RuBisCO small and large subunits and is implicated in the assembly of the enzyme oligomer. Involved in protein assisted folding. Required for proper plastid division.5 Publications

Miscellaneous

Assisted protein folding requires ATP hydrolysis, but not K+ ions.

GO - Molecular functioni

GO - Biological processi

  • cell death Source: TAIR
  • chaperone cofactor-dependent protein refolding Source: TAIR
  • mitochondrion organization Source: GO_Central
  • protein refolding Source: InterPro
  • response to cold Source: TAIR
  • systemic acquired resistance Source: TAIR

Keywordsi

Molecular functionChaperone
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperonin 60 subunit beta 1, chloroplastic
Short name:
CPN-60 beta 1
Alternative name(s):
60 kDa chaperonin subunit beta 1
RuBisCO large subunit-binding protein subunit beta, chloroplastic
Gene namesi
Name:CPN60B1
Synonyms:Cpn60-B(3), LEN1
Ordered Locus Names:At1g55490
ORF Names:T5A14.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G55490
TAIRilocus:2193839 AT1G55490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Normal germination, but chloroplast-division defect and late dwarf phenotype. Lesion formation on leaves when grown under short-day conditions. Cpn60B1 and cpn60B2 double mutant produces small albino seedlings.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 54Chloroplast1 PublicationAdd BLAST54
ChainiPRO_000000502255 – 600Chaperonin 60 subunit beta 1, chloroplasticAdd BLAST546

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei101PhosphoserineBy similarity1
Modified residuei478PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP21240
PRIDEiP21240

PTM databases

iPTMnetiP21240

Expressioni

Tissue specificityi

Expressed in leaves, stems, petioles and flowers.1 Publication

Inductioni

Up-regulated by light. Down-regulated by wounding. Not induced by heat.2 Publications

Gene expression databases

ExpressionAtlasiP21240 baseline and differential
GenevisibleiP21240 AT

Interactioni

Subunit structurei

Part of the Cpn60 complex composed of 7 alpha and 7 beta subunits. Can also form a complex composed of 14 beta subunits only. Both complexes show ATPase activity. The Cpn60 complex interacts with the Cpn10 complex. Interacts with RAB during heat stress.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi27221, 10 interactors
IntActiP21240, 2 interactors
MINTiP21240
STRINGi3702.AT1G55490.1

Structurei

3D structure databases

ProteinModelPortaliP21240
SMRiP21240
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356 Eukaryota
COG0459 LUCA
HOGENOMiHOG000076290
InParanoidiP21240
KOiK04077
OMAiIVCNDGV
OrthoDBiEOG093606CX
PhylomeDBiP21240

Family and domain databases

CDDicd03344 GroEL, 1 hit
Gene3Di1.10.560.10, 2 hits
3.30.260.10, 2 hits
3.50.7.10, 1 hit
HAMAPiMF_00600 CH60, 1 hit
InterProiView protein in InterPro
IPR018370 Chaperonin_Cpn60_CS
IPR001844 Chaprnin_Cpn60
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00298 CHAPERONIN60
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 1 hit
TIGRFAMsiTIGR02348 GroEL, 1 hit
PROSITEiView protein in PROSITE
PS00296 CHAPERONINS_CPN60, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21240-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASTFTATSS IGSMVAPNGH KSDKKLISKL SSSSFGRRQS VCPRPRRSSS
60 70 80 90 100
AIVCAAKELH FNKDGTTIRR LQAGVNKLAD LVGVTLGPKG RNVVLESKYG
110 120 130 140 150
SPRIVNDGVT VAREVELEDP VENIGAKLVR QAAAKTNDLA GDGTTTSVVL
160 170 180 190 200
AQGFIAEGVK VVAAGANPVL ITRGIEKTAK ALVTELKKMS KEVEDSELAD
210 220 230 240 250
VAAVSAGNND EIGNMIAEAM SKVGRKGVVT LEEGKSAENN LYVVEGMQFD
260 270 280 290 300
RGYISPYFVT DSEKMSVEFD NCKLLLVDKK ITNARDLVGV LEDAIRGGYP
310 320 330 340 350
ILIIAEDIEQ EALATLVVNK LRGTLKIAAL RAPGFGERKS QYLDDIAILT
360 370 380 390 400
GATVIREEVG LSLDKAGKEV LGNASKVVLT KETSTIVGDG STQDAVKKRV
410 420 430 440 450
TQIKNLIEQA EQDYEKEKLN ERIAKLSGGV AVIQVGAQTE TELKEKKLRV
460 470 480 490 500
EDALNATKAA VEEGIVVGGG CTLLRLASKV DAIKATLDND EEKVGADIVK
510 520 530 540 550
RALSYPLKLI AKNAGVNGSV VSEKVLSNDN VKFGYNAATG KYEDLMAAGI
560 570 580 590 600
IDPTKVVRCC LEHAASVAKT FLMSDCVVVE IKEPEPVPVG NPMDNSGYGY
Length:600
Mass (Da):63,809
Last modified:January 10, 2003 - v3
Checksum:i78B10E9B2EE19859
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti242Y → H in AAA32725 (PubMed:11130712).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005223 Genomic DNA Translation: AAD10647.1
CP002684 Genomic DNA Translation: AEE33251.1
CP002684 Genomic DNA Translation: AEE33252.1
CP002684 Genomic DNA Translation: ANM58183.1
CP002684 Genomic DNA Translation: ANM58184.1
CP002684 Genomic DNA Translation: ANM58185.1
AF386945 mRNA Translation: AAK62390.1
AY081501 mRNA Translation: AAM10063.1
AK316889 mRNA Translation: BAH19596.1
AK317612 mRNA Translation: BAH20275.1
M35598 mRNA Translation: AAA32725.1
PIRiB96597
JT0901
PW0008
RefSeqiNP_001320637.1, NM_001333712.1
NP_001320638.1, NM_001333711.1
NP_001320639.1, NM_001333710.1
NP_175945.1, NM_104424.2
NP_849811.1, NM_179480.3
UniGeneiAt.19230

Genome annotation databases

EnsemblPlantsiAT1G55490.1; AT1G55490.1; AT1G55490
AT1G55490.2; AT1G55490.2; AT1G55490
AT1G55490.3; AT1G55490.3; AT1G55490
AT1G55490.4; AT1G55490.4; AT1G55490
AT1G55490.5; AT1G55490.5; AT1G55490
GeneIDi841996
GrameneiAT1G55490.1; AT1G55490.1; AT1G55490
AT1G55490.2; AT1G55490.2; AT1G55490
AT1G55490.3; AT1G55490.3; AT1G55490
AT1G55490.4; AT1G55490.4; AT1G55490
AT1G55490.5; AT1G55490.5; AT1G55490
KEGGiath:AT1G55490

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005223 Genomic DNA Translation: AAD10647.1
CP002684 Genomic DNA Translation: AEE33251.1
CP002684 Genomic DNA Translation: AEE33252.1
CP002684 Genomic DNA Translation: ANM58183.1
CP002684 Genomic DNA Translation: ANM58184.1
CP002684 Genomic DNA Translation: ANM58185.1
AF386945 mRNA Translation: AAK62390.1
AY081501 mRNA Translation: AAM10063.1
AK316889 mRNA Translation: BAH19596.1
AK317612 mRNA Translation: BAH20275.1
M35598 mRNA Translation: AAA32725.1
PIRiB96597
JT0901
PW0008
RefSeqiNP_001320637.1, NM_001333712.1
NP_001320638.1, NM_001333711.1
NP_001320639.1, NM_001333710.1
NP_175945.1, NM_104424.2
NP_849811.1, NM_179480.3
UniGeneiAt.19230

3D structure databases

ProteinModelPortaliP21240
SMRiP21240
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27221, 10 interactors
IntActiP21240, 2 interactors
MINTiP21240
STRINGi3702.AT1G55490.1

PTM databases

iPTMnetiP21240

Proteomic databases

PaxDbiP21240
PRIDEiP21240

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G55490.1; AT1G55490.1; AT1G55490
AT1G55490.2; AT1G55490.2; AT1G55490
AT1G55490.3; AT1G55490.3; AT1G55490
AT1G55490.4; AT1G55490.4; AT1G55490
AT1G55490.5; AT1G55490.5; AT1G55490
GeneIDi841996
GrameneiAT1G55490.1; AT1G55490.1; AT1G55490
AT1G55490.2; AT1G55490.2; AT1G55490
AT1G55490.3; AT1G55490.3; AT1G55490
AT1G55490.4; AT1G55490.4; AT1G55490
AT1G55490.5; AT1G55490.5; AT1G55490
KEGGiath:AT1G55490

Organism-specific databases

AraportiAT1G55490
TAIRilocus:2193839 AT1G55490

Phylogenomic databases

eggNOGiKOG0356 Eukaryota
COG0459 LUCA
HOGENOMiHOG000076290
InParanoidiP21240
KOiK04077
OMAiIVCNDGV
OrthoDBiEOG093606CX
PhylomeDBiP21240

Miscellaneous databases

PROiPR:P21240

Gene expression databases

ExpressionAtlasiP21240 baseline and differential
GenevisibleiP21240 AT

Family and domain databases

CDDicd03344 GroEL, 1 hit
Gene3Di1.10.560.10, 2 hits
3.30.260.10, 2 hits
3.50.7.10, 1 hit
HAMAPiMF_00600 CH60, 1 hit
InterProiView protein in InterPro
IPR018370 Chaperonin_Cpn60_CS
IPR001844 Chaprnin_Cpn60
IPR002423 Cpn60/TCP-1
IPR027409 GroEL-like_apical_dom_sf
IPR027413 GROEL-like_equatorial_sf
IPR027410 TCP-1-like_intermed_sf
PfamiView protein in Pfam
PF00118 Cpn60_TCP1, 1 hit
PRINTSiPR00298 CHAPERONIN60
SUPFAMiSSF48592 SSF48592, 2 hits
SSF52029 SSF52029, 1 hit
SSF54849 SSF54849, 1 hit
TIGRFAMsiTIGR02348 GroEL, 1 hit
PROSITEiView protein in PROSITE
PS00296 CHAPERONINS_CPN60, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCPNB1_ARATH
AccessioniPrimary (citable) accession number: P21240
Secondary accession number(s): B9DFS9, B9DHQ8, Q9SAV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 10, 2003
Last modified: May 23, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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