Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA polymerase II

Gene

polB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thought to be involved in DNA repair and/or mutagenesis. Its processivity is enhanced by the beta sliding clamp (dnaN) and clamp loader (PubMed:1999435, PubMed:1534562).2 Publications

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Activity regulationi

DNA polymerase II activity is regulated by the lexA gene during the SOS response.

GO - Molecular functioni

GO - Biological processi

  • DNA-dependent DNA replication Source: EcoCyc
  • DNA replication proofreading Source: EcoCyc
  • SOS response Source: EcoCyc

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, SOS response

Enzyme and pathway databases

BioCyciEcoCyc:EG10747-MONOMER
MetaCyc:EG10747-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase II (EC:2.7.7.7)
Short name:
Pol II
Gene namesi
Name:polB
Synonyms:dinA
Ordered Locus Names:b0060, JW0059
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10747 polB

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000464732 – 783DNA polymerase IIAdd BLAST782

Proteomic databases

PaxDbiP21189
PRIDEiP21189

Interactioni

Protein-protein interaction databases

BioGridi4261621, 107 interactors
IntActiP21189, 5 interactors
STRINGi316407.85674307

Structurei

Secondary structure

1783
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP21189
SMRiP21189
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP21189

Family & Domainsi

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated

Phylogenomic databases

eggNOGiENOG4105CQ2 Bacteria
COG0417 LUCA
HOGENOMiHOG000221829
InParanoidiP21189
KOiK02336
PhylomeDBiP21189

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.90.1600.10, 2 hits
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR017964 DNA-dir_DNA_pol_B_CS
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 2 hits
PRINTSiPR00106 DNAPOLB
SMARTiView protein in SMART
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS00116 DNA_POLYMERASE_B, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P21189-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAQAGFILTR HWRDTPQGTE VSFWLATDNG PLQVTLAPQE SVAFIPADQV
60 70 80 90 100
PRAQHILQGE QGFRLTPLAL KDFHRQPVYG LYCRAHRQLM NYEKRLREGG
110 120 130 140 150
VTVYEADVRP PERYLMERFI TSPVWVEGDM HNGTIVNARL KPHPDYRPPL
160 170 180 190 200
KWVSIDIETT RHGELYCIGL EGCGQRIVYM LGPENGDASS LDFELEYVAS
210 220 230 240 250
RPQLLEKLNA WFANYDPDVI IGWNVVQFDL RMLQKHAERY RLPLRLGRDN
260 270 280 290 300
SELEWREHGF KNGVFFAQAK GRLIIDGIEA LKSAFWNFSS FSLETVAQEL
310 320 330 340 350
LGEGKSIDNP WDRMDEIDRR FAEDKPALAT YNLKDCELVT QIFHKTEIMP
360 370 380 390 400
FLLERATVNG LPVDRHGGSV AAFGHLYFPR MHRAGYVAPN LGEVPPHASP
410 420 430 440 450
GGYVMDSRPG LYDSVLVLDY KSLYPSIIRT FLIDPVGLVE GMAQPDPEHS
460 470 480 490 500
TEGFLDAWFS REKHCLPEIV TNIWHGRDEA KRQGNKPLSQ ALKIIMNAFY
510 520 530 540 550
GVLGTTACRF FDPRLASSIT MRGHQIMRQT KALIEAQGYD VIYGDTDSTF
560 570 580 590 600
VWLKGAHSEE EAAKIGRALV QHVNAWWAET LQKQRLTSAL ELEYETHFCR
610 620 630 640 650
FLMPTIRGAD TGSKKRYAGL IQEGDKQRMV FKGLETVRTD WTPLAQQFQQ
660 670 680 690 700
ELYLRIFRNE PYQEYVRETI DKLMAGELDA RLVYRKRLRR PLSEYQRNVP
710 720 730 740 750
PHVRAARLAD EENQKRGRPL QYQNRGTIKY VWTTNGPEPL DYQRSPLDYE
760 770 780
HYLTRQLQPV AEGILPFIED NFATLMTGQL GLF
Length:783
Mass (Da):90,053
Last modified:January 23, 2007 - v2
Checksum:i1A12A21B2FDB0E22
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172G → A in AAA24406 (PubMed:2261080).Curated1
Sequence conflicti172G → A in AAA24407 (PubMed:2261080).Curated1
Sequence conflicti257 – 258EH → DD in AAA63764 (PubMed:2217198).Curated2
Sequence conflicti257 – 258EH → DD in AAA23684 (PubMed:2217198).Curated2
Sequence conflicti272R → G in AAA63764 (PubMed:2217198).Curated1
Sequence conflicti272R → G in AAA23684 (PubMed:2217198).Curated1
Sequence conflicti735N → T in AAA24406 (PubMed:2261080).Curated1
Sequence conflicti735N → T in AAA24407 (PubMed:2261080).Curated1
Sequence conflicti740 – 783LDYQR…QLGLF → PGLPTFTTGLRTLSDPPATT RGGGNTPFY in AAA24406 (PubMed:2261080).CuratedAdd BLAST44
Sequence conflicti740 – 783LDYQR…QLGLF → PGLPTFTTGLRTLSDPPATT RGGGNTPFY in AAA24407 (PubMed:2261080).CuratedAdd BLAST44

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti401G → D in allele POLB100. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54847 Genomic DNA Translation: CAA38616.1
M62646 Genomic DNA Translation: AAA24406.1
M35371 Genomic DNA Translation: AAA24407.1
M38283 Genomic DNA Translation: AAA63764.1
U00096 Genomic DNA Translation: AAC73171.1
AP009048 Genomic DNA Translation: BAB96628.2
M37727 Genomic DNA Translation: AAA23684.1
PIRiS15943 JDEC22
RefSeqiNP_414602.1, NC_000913.3
WP_000035670.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73171; AAC73171; b0060
BAB96628; BAB96628; BAB96628
GeneIDi944779
KEGGiecj:JW0059
eco:b0060
PATRICifig|1411691.4.peg.2223

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54847 Genomic DNA Translation: CAA38616.1
M62646 Genomic DNA Translation: AAA24406.1
M35371 Genomic DNA Translation: AAA24407.1
M38283 Genomic DNA Translation: AAA63764.1
U00096 Genomic DNA Translation: AAC73171.1
AP009048 Genomic DNA Translation: BAB96628.2
M37727 Genomic DNA Translation: AAA23684.1
PIRiS15943 JDEC22
RefSeqiNP_414602.1, NC_000913.3
WP_000035670.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q8IX-ray2.00A1-783[»]
3K57X-ray2.08A1-783[»]
3K58X-ray2.05A1-783[»]
3K59X-ray1.92A1-783[»]
3K5LX-ray2.70A1-783[»]
3K5MX-ray2.04A1-783[»]
3K5NX-ray3.15A/B1-783[»]
3K5OX-ray2.20A/B1-783[»]
3MAQX-ray2.40A1-783[»]
ProteinModelPortaliP21189
SMRiP21189
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261621, 107 interactors
IntActiP21189, 5 interactors
STRINGi316407.85674307

Proteomic databases

PaxDbiP21189
PRIDEiP21189

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73171; AAC73171; b0060
BAB96628; BAB96628; BAB96628
GeneIDi944779
KEGGiecj:JW0059
eco:b0060
PATRICifig|1411691.4.peg.2223

Organism-specific databases

EchoBASEiEB0740
EcoGeneiEG10747 polB

Phylogenomic databases

eggNOGiENOG4105CQ2 Bacteria
COG0417 LUCA
HOGENOMiHOG000221829
InParanoidiP21189
KOiK02336
PhylomeDBiP21189

Enzyme and pathway databases

BioCyciEcoCyc:EG10747-MONOMER
MetaCyc:EG10747-MONOMER

Miscellaneous databases

EvolutionaryTraceiP21189
PROiPR:P21189

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.90.1600.10, 2 hits
InterProiView protein in InterPro
IPR006172 DNA-dir_DNA_pol_B
IPR017964 DNA-dir_DNA_pol_B_CS
IPR006133 DNA-dir_DNA_pol_B_exonuc
IPR006134 DNA-dir_DNA_pol_B_multi_dom
IPR023211 DNA_pol_palm_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF00136 DNA_pol_B, 1 hit
PF03104 DNA_pol_B_exo1, 2 hits
PRINTSiPR00106 DNAPOLB
SMARTiView protein in SMART
SM00486 POLBc, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS00116 DNA_POLYMERASE_B, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPO2_ECOLI
AccessioniPrimary (citable) accession number: P21189
Secondary accession number(s): Q8KMX7, Q8KMX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 1991
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again