UniProtKB - P21187 (PABP_DROME)
Polyadenylate-binding protein
pAbp
Functioni
Binds the poly(A) tail of mRNA (PubMed:2125288).
Since it interacts with the cap-associating translation initiation factor eIF4G, it is likely that it functions by linking Atx2 to the cap-binding complex (PubMed:28388438).
Forms a complex with tyf and Atx2 which functions in adult circadian pacemaker neurons to sustain circadian rhythms likely by switching between activator and repressor modes of post-transcriptional regulation via interaction with Lsm12a or me31B, respectively (PubMed:28388438).
The activator complex (Atx2-tyf activator complex) activates the TYF-dependent translation of per to maintain 24 hour periodicity in circadian locomotor behaviors, whereas the repressor complex (Atx2-Not1 repressor complex) promotes Not1-dependent post-transcriptional gene silencing and supports high-amplitude circadian rhythms in a per-independent manner (PubMed:28388438).
In 0-1 hour embryos, forms a ribonucleoprotein complex (RNP) with me31B, eIF4E1, tral and cup which binds to various mRNAs including maternal mRNAs, and down-regulates their expression during the maternal-to-zygotic transition (PubMed:28875934).
3 PublicationsGO - Molecular functioni
- mRNA 3'-UTR binding Source: FlyBase
- mRNA binding Source: FlyBase
- poly(A) binding Source: FlyBase
- poly(U) RNA binding Source: GO_Central
- RNA binding Source: GO_Central
GO - Biological processi
- chemical synaptic transmission Source: FlyBase
- dorsal/ventral pattern formation Source: FlyBase
- male meiosis cytokinesis Source: FlyBase
- male meiotic nuclear division Source: FlyBase
- mRNA splicing, via spliceosome Source: FlyBase
- negative regulation of neuron death Source: FlyBase
- oocyte development Source: FlyBase
- oogenesis Source: FlyBase
- positive regulation of translation Source: FlyBase
- regulation of compound eye photoreceptor development Source: FlyBase
- spermatid nucleus differentiation Source: FlyBase
- spermatogenesis Source: FlyBase
Keywordsi
Molecular function | RNA-binding |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-429947, Deadenylation of mRNA R-DME-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-DME-72649, Translation initiation complex formation R-DME-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
SignaLinki | P21187 |
Names & Taxonomyi
Protein namesi | Recommended name: Polyadenylate-binding proteinShort name: PABP Short name: Poly(A)-binding protein |
Gene namesi | Name:pAbp ORF Names:CG5119 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0265297, pAbp |
VEuPathDBi | VectorBase:FBgn0265297 |
Subcellular locationi
Cytosol
- cytosol Source: FlyBase
Nucleus
- catalytic step 2 spliceosome Source: FlyBase
- nucleus Source: FlyBase
- precatalytic spliceosome Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- cytoplasmic stress granule Source: GO_Central
- ribonucleoprotein complex Source: FlyBase
- synapse Source: GOC
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081713 | 1 – 634 | Polyadenylate-binding proteinAdd BLAST | 634 |
Proteomic databases
PaxDbi | P21187 |
PRIDEi | P21187 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | FBgn0265297, Expressed in cleaving embryo and 41 other tissues |
Genevisiblei | P21187, DM |
Interactioni
Subunit structurei
Core component of the neuromuscular Atx2 complex, composed of at least Atx2, tyf, pAbp, Lsm12a (PubMed:28388438).
Interacts with Atx2 (via PAM2 motif) (PubMed:28388438). Atx2 and pAbp form a subcomplex that can associate with the 5' cap of pre-mRNAs independently of tyf, Lsm12a or me31B (PubMed:28388438).
Forms a ribonucleoprotein complex (RNP) containing at least me31B, eIF4E1, cup, tral and pAbp; this interaction is required for the translational silencing of maternal mRNAs during the maternal-to-zygotic transition (PubMed:28875934). In 1-5 hour embryos, interaction with me31B is severely reduced and there is no detected interaction with cup and tral, however interaction with eIF4E1 and eIF4G1 remains unchanged (PubMed:28875934).
Interacts with gw; this interaction interferes with the binding of pAbp to eIF4G and is required for miRNA-mediated silencing (PubMed:19797087).
Interacts with larp (PubMed:19631203).
4 PublicationsBinary interactionsi
P21187
Protein-protein interaction databases
BioGRIDi | 62753, 66 interactors |
DIPi | DIP-52140N |
ELMi | P21187 |
IntActi | P21187, 18 interactors |
MINTi | P21187 |
STRINGi | 7227.FBpp0085915 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 80 | RRM 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 90 – 167 | RRM 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 181 – 259 | RRM 3PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 285 – 362 | RRM 4PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 552 – 629 | PABCPROSITE-ProRule annotationAdd BLAST | 78 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0123, Eukaryota |
GeneTreei | ENSGT00940000169027 |
HOGENOMi | CLU_012062_22_2_1 |
InParanoidi | P21187 |
OMAi | QYMQPPV |
OrthoDBi | 1027234at2759 |
PhylomeDBi | P21187 |
Family and domain databases
CDDi | cd12379, RRM2_I_PABPs, 1 hit |
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR036053, PABP-dom IPR006515, PABP_1234 IPR002004, PABP_HYD IPR035979, RBD_domain_sf IPR045305, RRM2_I_PABPs IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00658, PABP, 1 hit PF00076, RRM_1, 4 hits |
SMARTi | View protein in SMART SM00517, PolyA, 1 hit SM00360, RRM, 4 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits SSF63570, SSF63570, 1 hit |
TIGRFAMsi | TIGR01628, PABP-1234, 1 hit |
PROSITEi | View protein in PROSITE PS51309, PABC, 1 hit PS50102, RRM, 4 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MASLYVGDLP QDVNESGLFD KFSSAGPVLS IRVCRDVITR RSLGYAYVNF
60 70 80 90 100
QQPADAERAL DTMNFDLVRN KPIRIMWSQR DPSLRRSGVG NVFIKNLDRA
110 120 130 140 150
IDNKAIYDTF SAFGNILSCK VATDEKGNSK GYGFVHFETE EAANTSIDKV
160 170 180 190 200
NGMLLNGKKV YVGKFIPRKE REKELGEKAK LFTNVYVKNF TEDFDDEKLK
210 220 230 240 250
EFFEPYGKIT SYKVMSKEDG KSKGFGFVAF ETTEAAEAAV QALNGKDMGE
260 270 280 290 300
GKSLYVARAQ KKAERQQELK RKFEELKQKR HESVFGVNLY VKNLDDTIDD
310 320 330 340 350
DRLRIAFSPY GNITSAKVMT DEEGRSKGFG FVCFNAASEA TCAVTELNGR
360 370 380 390 400
VVGSKPLYVA LAQRKEERKA HLASQYMRHM TGMRMQQLGQ IYQPNAASGF
410 420 430 440 450
FVPTLPSNQR FFGSQVATQM RNTPRWVPQV RPPAAIQGVQ AGAAAAGGFQ
460 470 480 490 500
GTAGAVPTQF RSAAAGARGA QPQVQGTHAA AAAANNMRNT GARAITGQQT
510 520 530 540 550
AAPNMQIPGA QIAGGAQQRT SNYKYTSNMR NPPVPQLHQT QPIPQQLQGK
560 570 580 590 600
NSEKLIASLL ANAKPQEQKQ ILGERLYPMI EHMHANLAGK ITGMLLEIEN
610 620 630
SELLHMIEDQ EALKAKVEEA VAVLQVHRVT EPAN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 171 – 173 | REK → Q in AAA70421 (PubMed:2125288).Curated | 3 | |
Sequence conflicti | 336 – 337 | AA → PE in AAA70421 (PubMed:2125288).Curated | 2 | |
Sequence conflicti | 371 | H → D in AAA70421 (PubMed:2125288).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L05109 mRNA Translation: AAA70421.1 AE013599 Genomic DNA Translation: AAF57746.1 AE013599 Genomic DNA Translation: AAM68175.1 AY118528 mRNA Translation: AAM49897.1 |
PIRi | S30887 |
RefSeqi | NP_476667.1, NM_057319.5 NP_725749.1, NM_166264.4 NP_725750.1, NM_166265.2 |
Genome annotation databases
EnsemblMetazoai | FBtr0086736; FBpp0085915; FBgn0265297 FBtr0086738; FBpp0085917; FBgn0265297 FBtr0086740; FBpp0085919; FBgn0265297 |
GeneIDi | 37070 |
KEGGi | dme:Dmel_CG5119 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L05109 mRNA Translation: AAA70421.1 AE013599 Genomic DNA Translation: AAF57746.1 AE013599 Genomic DNA Translation: AAM68175.1 AY118528 mRNA Translation: AAM49897.1 |
PIRi | S30887 |
RefSeqi | NP_476667.1, NM_057319.5 NP_725749.1, NM_166264.4 NP_725750.1, NM_166265.2 |
3D structure databases
SMRi | P21187 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 62753, 66 interactors |
DIPi | DIP-52140N |
ELMi | P21187 |
IntActi | P21187, 18 interactors |
MINTi | P21187 |
STRINGi | 7227.FBpp0085915 |
Proteomic databases
PaxDbi | P21187 |
PRIDEi | P21187 |
Protocols and materials databases
ABCDi | P21187, 15 sequenced antibodies |
DNASUi | 37070 |
Genome annotation databases
EnsemblMetazoai | FBtr0086736; FBpp0085915; FBgn0265297 FBtr0086738; FBpp0085917; FBgn0265297 FBtr0086740; FBpp0085919; FBgn0265297 |
GeneIDi | 37070 |
KEGGi | dme:Dmel_CG5119 |
Organism-specific databases
CTDi | 37070 |
FlyBasei | FBgn0265297, pAbp |
VEuPathDBi | VectorBase:FBgn0265297 |
Phylogenomic databases
eggNOGi | KOG0123, Eukaryota |
GeneTreei | ENSGT00940000169027 |
HOGENOMi | CLU_012062_22_2_1 |
InParanoidi | P21187 |
OMAi | QYMQPPV |
OrthoDBi | 1027234at2759 |
PhylomeDBi | P21187 |
Enzyme and pathway databases
Reactomei | R-DME-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-DME-429947, Deadenylation of mRNA R-DME-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-DME-72649, Translation initiation complex formation R-DME-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-DME-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
SignaLinki | P21187 |
Miscellaneous databases
BioGRID-ORCSi | 37070, 1 hit in 3 CRISPR screens |
ChiTaRSi | pAbp, fly |
GenomeRNAii | 37070 |
PROi | PR:P21187 |
Gene expression databases
Bgeei | FBgn0265297, Expressed in cleaving embryo and 41 other tissues |
Genevisiblei | P21187, DM |
Family and domain databases
CDDi | cd12379, RRM2_I_PABPs, 1 hit |
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR036053, PABP-dom IPR006515, PABP_1234 IPR002004, PABP_HYD IPR035979, RBD_domain_sf IPR045305, RRM2_I_PABPs IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00658, PABP, 1 hit PF00076, RRM_1, 4 hits |
SMARTi | View protein in SMART SM00517, PolyA, 1 hit SM00360, RRM, 4 hits |
SUPFAMi | SSF54928, SSF54928, 2 hits SSF63570, SSF63570, 1 hit |
TIGRFAMsi | TIGR01628, PABP-1234, 1 hit |
PROSITEi | View protein in PROSITE PS51309, PABC, 1 hit PS50102, RRM, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PABP_DROME | |
Accessioni | P21187Primary (citable) accession number: P21187 Secondary accession number(s): A4UZM5, Q0E932, Q9V8C3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | September 27, 2005 | |
Last modified: | February 23, 2022 | |
This is version 197 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families