UniProtKB - P21177 (FADB_ECOLI)
Protein
Fatty acid oxidation complex subunit alpha
Gene
fadB
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.UniRule annotation5 Publications
Catalytic activityi
- EC:1.1.1.35UniRule annotation
- EC:4.2.1.17UniRule annotation
- EC:4.2.1.17UniRule annotation
- EC:5.1.2.3UniRule annotation
- EC:5.3.3.8UniRule annotation
- EC:5.3.3.8UniRule annotation
Kineticsi
- KM=53 µM for crotonyl-CoA (for enoyl-CoA hydratase activity)3 Publications
- KM=8.7 mM for L-3-hydroxy-4-trans-decenoyl-CoA (for enoyl-CoA hydratase activity)3 Publications
- KM=38 mM for D-3-hydroxy-4-trans-decenoyl-CoA (for enoyl-CoA hydratase activity)3 Publications
- KM=5.8 µM for 3-cis-tetradecenoyl-CoA (for Delta3-cis-Delta2-trans-enoyl-CoA isomerase activity)3 Publications
- KM=69 µM for acetoacetyl-CoA (for 3-hydroxyacyl-CoA dehydrogenase activity)3 Publications
- KM=2.0 µM for NADH (for 3-hydroxyacyl-CoA dehydrogenase activity)3 Publications
: fatty acid beta-oxidation Pathwayi
This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotationView all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 119 | Important for catalytic activityUniRule annotation | 1 | |
Sitei | 139 | Important for catalytic activityUniRule annotation | 1 | |
Binding sitei | 296 | SubstrateUniRule annotation | 1 | |
Binding sitei | 324 | NAD; via amide nitrogenUniRule annotationCurated | 1 | |
Binding sitei | 343 | NADUniRule annotation | 1 | |
Binding sitei | 407 | NADUniRule annotation | 1 | |
Binding sitei | 429 | NADUniRule annotation | 1 | |
Active sitei | 450 | For 3-hydroxyacyl-CoA dehydrogenase activityUniRule annotation2 Publications | 1 | |
Binding sitei | 453 | NADUniRule annotation | 1 | |
Binding sitei | 500 | SubstrateUniRule annotation | 1 | |
Binding sitei | 660 | SubstrateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 400 – 402 | NADUniRule annotation | 3 |
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB
- 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB
- dodecenoyl-CoA delta-isomerase activity Source: UniProtKB
- enoyl-CoA hydratase activity Source: UniProtKB
- long-chain-3-hydroxyacyl-CoA dehydrogenase activity Source: GO_Central
GO - Biological processi
- fatty acid beta-oxidation Source: EcoCyc
Keywordsi
Molecular function | Isomerase, Lyase, Multifunctional enzyme, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid degradation, Lipid metabolism |
Ligand | NAD |
Enzyme and pathway databases
BioCyci | EcoCyc:FADB-MONOMER MetaCyc:FADB-MONOMER |
SABIO-RKi | P21177 |
UniPathwayi | UPA00659 |
Names & Taxonomyi
Protein namesi | Recommended name: Fatty acid oxidation complex subunit alphaUniRule annotationIncluding the following 2 domains: |
Gene namesi | Name:fadBUniRule annotation Synonyms:oldB Ordered Locus Names:b3846, JW3822 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- fatty acid beta-oxidation multienzyme complex Source: InterPro
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 116 | G → F: Absence of both enoyl-CoA hydratase and 3-hydroxyacyl-CoA epimerase activities. Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase is only slightly affected. 1 Publication | 1 | |
Mutagenesisi | 322 | G → A: 10-fold increase in KM for NADH. 1 Publication | 1 | |
Mutagenesisi | 450 | H → A or Q: Almost complete loss of 3-hydroxyacyl-CoA dehydrogenase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000109268 | 1 – 729 | Fatty acid oxidation complex subunit alphaAdd BLAST | 729 |
Proteomic databases
jPOSTi | P21177 |
PaxDbi | P21177 |
PRIDEi | P21177 |
Expressioni
Inductioni
Repressed by FadR in the absence of LCFAs (fatty acids of, at least, 12 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs which bind to FadR resulting in its release from the DNA and thus derepression of the transcription.
Interactioni
Subunit structurei
Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).
UniRule annotation1 PublicationProtein-protein interaction databases
BioGRIDi | 4263448, 158 interactors |
ComplexPortali | CPX-3964, fadBA fatty acid oxidation complex, aerobic conditions |
DIPi | DIP-9560N |
IntActi | P21177, 7 interactors |
STRINGi | 511145.b3846 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SASBDBi | P21177 |
SMRi | P21177 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 189 | Enoyl-CoA hydratase/isomeraseUniRule annotationAdd BLAST | 189 | |
Regioni | 311 – 729 | 3-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST | 419 |
Sequence similaritiesi
In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG1024, Bacteria COG1250, Bacteria |
HOGENOMi | CLU_009834_16_3_6 |
InParanoidi | P21177 |
PhylomeDBi | P21177 |
Family and domain databases
HAMAPi | MF_01621, FadB, 1 hit |
InterProi | View protein in InterPro IPR006180, 3-OHacyl-CoA_DH_CS IPR006176, 3-OHacyl-CoA_DH_NAD-bd IPR006108, 3HC_DH_C IPR008927, 6-PGluconate_DH-like_C_sf IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR012799, FadB IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00725, 3HCDH, 2 hits PF02737, 3HCDH_N, 1 hit PF00378, ECH_1, 1 hit |
SUPFAMi | SSF48179, SSF48179, 2 hits SSF51735, SSF51735, 1 hit SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR02437, FadB, 1 hit |
PROSITEi | View protein in PROSITE PS00067, 3HCDH, 1 hit PS00166, ENOYL_COA_HYDRATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P21177-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLYKGDTLYL DWLEDGIAEL VFDAPGSVNK LDTATVASLG EAIGVLEQQS
60 70 80 90 100
DLKGLLLRSN KAAFIVGADI TEFLSLFLVP EEQLSQWLHF ANSVFNRLED
110 120 130 140 150
LPVPTIAAVN GYALGGGCEC VLATDYRLAT PDLRIGLPET KLGIMPGFGG
160 170 180 190 200
SVRMPRMLGA DSALEIIAAG KDVGADQALK IGLVDGVVKA EKLVEGAKAV
210 220 230 240 250
LRQAINGDLD WKAKRQPKLE PLKLSKIEAT MSFTIAKGMV AQTAGKHYPA
260 270 280 290 300
PITAVKTIEA AARFGREEAL NLENKSFVPL AHTNEARALV GIFLNDQYVK
310 320 330 340 350
GKAKKLTKDV ETPKQAAVLG AGIMGGGIAY QSAWKGVPVV MKDINDKSLT
360 370 380 390 400
LGMTEAAKLL NKQLERGKID GLKLAGVIST IHPTLDYAGF DRVDIVVEAV
410 420 430 440 450
VENPKVKKAV LAETEQKVRQ DTVLASNTST IPISELANAL ERPENFCGMH
460 470 480 490 500
FFNPVHRMPL VEIIRGEKSS DETIAKVVAW ASKMGKTPIV VNDCPGFFVN
510 520 530 540 550
RVLFPYFAGF SQLLRDGADF RKIDKVMEKQ FGWPMGPAYL LDVVGIDTAH
560 570 580 590 600
HAQAVMAAGF PQRMQKDYRD AIDALFDANR FGQKNGLGFW RYKEDSKGKP
610 620 630 640 650
KKEEDAAVED LLAEVSQPKR DFSEEEIIAR MMIPMVNEVV RCLEEGIIAT
660 670 680 690 700
PAEADMALVY GLGFPPFHGG AFRWLDTLGS AKYLDMAQQY QHLGPLYEVP
710 720
EGLRNKARHN EPYYPPVEPA RPVGDLKTA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 518 | A → R in AAA23750 (PubMed:1699931).Curated | 1 | |
Sequence conflicti | 664 | F → L in CAB40809 (PubMed:2204034).Curated | 1 | |
Sequence conflicti | 666 | P → A in CAB40809 (PubMed:2204034).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59368 Genomic DNA Translation: AAA23750.1 X52837 Genomic DNA Translation: CAB40809.1 M74164 Genomic DNA Translation: AAA62777.1 M87049 Genomic DNA Translation: AAA67643.1 U00096 Genomic DNA Translation: AAC76849.1 AP009048 Genomic DNA Translation: BAE77457.1 |
PIRi | A39592 |
RefSeqi | NP_418288.1, NC_000913.3 WP_000965936.1, NZ_SSZK01000046.1 |
Genome annotation databases
EnsemblBacteriai | AAC76849; AAC76849; b3846 BAE77457; BAE77457; BAE77457 |
GeneIDi | 948336 |
KEGGi | ecj:JW3822 eco:b3846 |
PATRICi | fig|511145.12.peg.3960 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59368 Genomic DNA Translation: AAA23750.1 X52837 Genomic DNA Translation: CAB40809.1 M74164 Genomic DNA Translation: AAA62777.1 M87049 Genomic DNA Translation: AAA67643.1 U00096 Genomic DNA Translation: AAC76849.1 AP009048 Genomic DNA Translation: BAE77457.1 |
PIRi | A39592 |
RefSeqi | NP_418288.1, NC_000913.3 WP_000965936.1, NZ_SSZK01000046.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6TNM | X-ray | 2.95 | A | 1-729 | [»] | |
SASBDBi | P21177 | |||||
SMRi | P21177 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263448, 158 interactors |
ComplexPortali | CPX-3964, fadBA fatty acid oxidation complex, aerobic conditions |
DIPi | DIP-9560N |
IntActi | P21177, 7 interactors |
STRINGi | 511145.b3846 |
Proteomic databases
jPOSTi | P21177 |
PaxDbi | P21177 |
PRIDEi | P21177 |
Genome annotation databases
EnsemblBacteriai | AAC76849; AAC76849; b3846 BAE77457; BAE77457; BAE77457 |
GeneIDi | 948336 |
KEGGi | ecj:JW3822 eco:b3846 |
PATRICi | fig|511145.12.peg.3960 |
Organism-specific databases
EchoBASEi | EB0275 |
Phylogenomic databases
eggNOGi | COG1024, Bacteria COG1250, Bacteria |
HOGENOMi | CLU_009834_16_3_6 |
InParanoidi | P21177 |
PhylomeDBi | P21177 |
Enzyme and pathway databases
UniPathwayi | UPA00659 |
BioCyci | EcoCyc:FADB-MONOMER MetaCyc:FADB-MONOMER |
SABIO-RKi | P21177 |
Miscellaneous databases
PROi | PR:P21177 |
Family and domain databases
HAMAPi | MF_01621, FadB, 1 hit |
InterProi | View protein in InterPro IPR006180, 3-OHacyl-CoA_DH_CS IPR006176, 3-OHacyl-CoA_DH_NAD-bd IPR006108, 3HC_DH_C IPR008927, 6-PGluconate_DH-like_C_sf IPR029045, ClpP/crotonase-like_dom_sf IPR018376, Enoyl-CoA_hyd/isom_CS IPR001753, Enoyl-CoA_hydra/iso IPR012799, FadB IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00725, 3HCDH, 2 hits PF02737, 3HCDH_N, 1 hit PF00378, ECH_1, 1 hit |
SUPFAMi | SSF48179, SSF48179, 2 hits SSF51735, SSF51735, 1 hit SSF52096, SSF52096, 1 hit |
TIGRFAMsi | TIGR02437, FadB, 1 hit |
PROSITEi | View protein in PROSITE PS00067, 3HCDH, 1 hit PS00166, ENOYL_COA_HYDRATASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FADB_ECOLI | |
Accessioni | P21177Primary (citable) accession number: P21177 Secondary accession number(s): Q2M8E9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1991 |
Last sequence update: | August 1, 1992 | |
Last modified: | December 2, 2020 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families