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Protein

Tropomyosin alpha-3 chain

Gene

Tpm3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.By similarity

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.Curated

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Muscle protein

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin alpha-3 chain
Alternative name(s):
Gamma-tropomyosin
Tropomyosin-3
Gene namesi
Name:Tpm3
Synonyms:Tpm-5, Tpm5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1890149 Tpm3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002056332 – 285Tropomyosin alpha-3 chainAdd BLAST284
Isoform 2 (identifier: P21107-2)
Initiator methionineiRemovedBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylmethionineBy similarity1
Modified residuei54PhosphothreonineBy similarity1
Modified residuei62PhosphoserineBy similarity1
Modified residuei88PhosphoserineBy similarity1
Modified residuei109PhosphothreonineBy similarity1
Modified residuei207PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei253PhosphothreonineBy similarity1
Modified residuei262PhosphotyrosineBy similarity1
Modified residuei272PhosphoserineCombined sources1
Modified residuei283PhosphothreonineBy similarity1
Modified residuei284PhosphoserineCombined sources1
Isoform 2 (identifier: P21107-2)
Modified residuei2N-acetylalanineBy similarity1
Modified residuei228N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP21107
MaxQBiP21107
PeptideAtlasiP21107
PRIDEiP21107

2D gel databases

COMPLUYEAST-2DPAGEiP21107
REPRODUCTION-2DPAGEiP21107
SWISS-2DPAGEiP21107

PTM databases

iPTMnetiP21107
PhosphoSitePlusiP21107
SwissPalmiP21107

Expressioni

Gene expression databases

BgeeiENSMUSG00000027940
CleanExiMM_TPM3
ExpressionAtlasiP21107 baseline and differential
GenevisibleiP21107 MM

Interactioni

Subunit structurei

Homodimer. Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain. Interacts with TMOD1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208522, 6 interactors
DIPiDIP-32061N
IntActiP21107, 6 interactors
MINTiP21107

Structurei

3D structure databases

ProteinModelPortaliP21107
SMRiP21107
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 285By similarityAdd BLAST285

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00550000074494
HOGENOMiHOG000231521
HOVERGENiHBG107404
InParanoidiP21107
KOiK09290

Family and domain databases

InterProiView protein in InterPro
IPR000533 Tropomyosin
PfamiView protein in Pfam
PF00261 Tropomyosin, 1 hit
PRINTSiPR00194 TROPOMYOSIN
PROSITEiView protein in PROSITE
PS00326 TROPOMYOSIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P21107-1) [UniParc]FASTAAdd to basket
Also known as: Skeletal muscle

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMEAIKKKMQ MLKLDKENVL DRAEQAEAEQ KQAEERSKQL EDELATMQKK
60 70 80 90 100
LKGTEDELDK YSEALKDAQE KLELAEKKAA DAEAEVASLN RRIQLVEEEL
110 120 130 140 150
DRAQERLATA LQKLEEAEKA ADESERGMKV IENRALKDEE KMELQEIQLK
160 170 180 190 200
EAKHIAEEAD RKYEEVARKL VIIEGDLERT EERAELAESK CSELEEELKN
210 220 230 240 250
VTNNLKSLEA QAEKYSQKED KYEEEIKILT DKLKEAETRA EFAERSVAKL
260 270 280
EKTIDDLEDE LYAQKLKYKA ISDELDHALN DMTSI
Length:285
Mass (Da):32,994
Last modified:June 26, 2013 - v3
Checksum:iF4407C132303002C
GO
Isoform 2 (identifier: P21107-2) [UniParc]FASTAAdd to basket
Also known as: Cytoskeletal

The sequence of this isoform differs from the canonical sequence as follows:
     1-81: MMEAIKKKMQ...LELAEKKAAD → MAGTTTIEAV...VEGERRAREQ
     190-212: KCSELEEELKNVTNNLKSLEAQA → RCREMDEQIRLMDQNLKCLSAAE
     260-285: ELYAQKLKYKAISDELDHALNDMTSI → KLKCTKEEHLCTQRMLDQTLLDLNEM

Show »
Length:248
Mass (Da):29,021
Checksum:i90F49312A655F53D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19V → F in AAA03725 (Ref. 2) Curated1
Sequence conflicti112Q → R in AAA03725 (Ref. 2) Curated1
Sequence conflicti181E → G in AAA03725 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0066081 – 81MMEAI…KKAAD → MAGTTTIEAVKRKIQVLQQQ ADDAEERAERLQREVEGERR AREQ in isoform 2. 2 PublicationsAdd BLAST81
Alternative sequenceiVSP_006609190 – 212KCSEL…LEAQA → RCREMDEQIRLMDQNLKCLS AAE in isoform 2. 2 PublicationsAdd BLAST23
Alternative sequenceiVSP_006610260 – 285ELYAQ…DMTSI → KLKCTKEEHLCTQRMLDQTL LDLNEM in isoform 2. 2 PublicationsAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53753 mRNA Translation: CAA37782.1
U04541 mRNA Translation: AAA03725.1
AF317223 mRNA Translation: AAG38596.1
AK088111 mRNA Translation: BAC40150.1
AK088902 mRNA Translation: BAC40643.1
X72633 mRNA Translation: CAA51209.1
CCDSiCCDS57223.1 [P21107-2]
PIRiI48852
S11390
RefSeqiNP_001240667.1, NM_001253738.1 [P21107-2]
NP_001280677.2, NM_001293748.1 [P21107-1]
UniGeneiMm.240839
Mm.421791
Mm.442249
Mm.490309

Genome annotation databases

EnsembliENSMUST00000029549; ENSMUSP00000029549; ENSMUSG00000027940 [P21107-2]
GeneIDi59069
KEGGimmu:59069
UCSCiuc008qbf.3 mouse [P21107-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTPM3_MOUSE
AccessioniPrimary (citable) accession number: P21107
Secondary accession number(s): Q09021
, Q60606, Q80SW6, Q9EPW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: June 26, 2013
Last modified: June 20, 2018
This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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