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Protein

Protein E3

Gene

E3L

Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

The C-terminus binds to and sequesters double-stranded RNA (dsRNA) synthesized during viral infection. This binding acts to 'mask' the dsRNA thereby preventing recognition and subsequent activation of EIF2AK2/PKR. The N-terminus is required for phosphorylation regulation of the translation initiation factor EIF2S1, but without affecting cytosolic protein translation. Blocks the phosphorylation and subsequent activation of IRF3 and IRF7 kinases, that are required for interferon-alpha (IFN-alpha) gene expression. Also inhibits NF-kappa-B activation and the ubiquitin-like protein G1P2/ISG15, which is an early antiviral protein (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host IRF3 by virus, Inhibition of host IRF7 by virus, Inhibition of host ISG15 by virus, Inhibition of host PKR by virus, Inhibition of host RLR pathway by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Protein E3
Alternative name(s):
p25
Gene namesi
ORF Names:E3L
OrganismiVaccinia virus (strain Copenhagen) (VACV)
Taxonomic identifieri10249 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008269 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000994471 – 190Protein E3Add BLAST190

Expressioni

Developmental stagei

Detected at early times, by 2 hours post infection, peaks at 5 hours post infection, and decreases during the late phase of virus replication.

Structurei

3D structure databases

ProteinModelPortaliP21081
SMRiP21081
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 73DRADAAdd BLAST69
Domaini117 – 184DRBMPROSITE-ProRule annotationAdd BLAST68

Sequence similaritiesi

Belongs to the poxviridae E3 protein family.Curated

Phylogenomic databases

OrthoDBiVOG090001IU

Family and domain databases

CDDicd00048 DSRM, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR000607 dsRNA_A_deaminase
IPR009179 E3L
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF02295 z-alpha, 1 hit
PIRSFiPIRSF004008 VAC_E3L, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00550 Zalpha, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50139 DRADA_REPEAT, 1 hit
PS50137 DS_RBD, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket
Isoform Long (identifier: P21081-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSKIYIDERS DAEIVCAAIK NIGIEGATAA QLTRQLNMEK REVNKALYDL
60 70 80 90 100
QRSAMVYSSD DIPPRWFMTT EADKPDADAM ADVIIDDVSR EKSMREDHKS
110 120 130 140 150
FDDVIPAKKI IDWKDANPVT IINEYCQITK RDWSFRIESV GPSNSPTFYA
160 170 180 190
CVDIDGRVFD KADGKSKRDA KNNAAKLAVD KLLGYVIIRF
Length:190
Mass (Da):21,504
Last modified:February 1, 1991 - v1
Checksum:i470D0610ACB4D44F
GO
Isoform Short (identifier: P21081-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-37: Missing.

Show »
Length:153
Mass (Da):17,515
Checksum:i9218A626626E1D9B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0189561 – 37Missing in isoform Short. CuratedAdd BLAST37

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35027 Genomic DNA Translation: AAA48040.1
PIRiG42508

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35027 Genomic DNA Translation: AAA48040.1
PIRiG42508

3D structure databases

ProteinModelPortaliP21081
SMRiP21081
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090001IU

Family and domain databases

CDDicd00048 DSRM, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR000607 dsRNA_A_deaminase
IPR009179 E3L
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00035 dsrm, 1 hit
PF02295 z-alpha, 1 hit
PIRSFiPIRSF004008 VAC_E3L, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 1 hit
SM00550 Zalpha, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS50139 DRADA_REPEAT, 1 hit
PS50137 DS_RBD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiE3_VACCC
AccessioniPrimary (citable) accession number: P21081
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 12, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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