UniProtKB - P20959 (IBP3_BOVIN)
Protein
Insulin-like growth factor-binding protein 3
Gene
IGFBP3
Organism
Bos taurus (Bovine)
Status
Functioni
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.
GO - Molecular functioni
- fibronectin binding Source: GO_Central
- insulin-like growth factor I binding Source: GO_Central
- insulin-like growth factor II binding Source: GO_Central
- protein tyrosine phosphatase activator activity Source: UniProtKB
GO - Biological processi
- negative regulation of protein phosphorylation Source: Ensembl
- negative regulation of smooth muscle cell migration Source: Ensembl
- negative regulation of smooth muscle cell proliferation Source: Ensembl
- osteoblast differentiation Source: Ensembl
- positive regulation of apoptotic process Source: UniProtKB
- positive regulation of insulin-like growth factor receptor signaling pathway Source: Ensembl
- positive regulation of MAPK cascade Source: Ensembl
- positive regulation of myoblast differentiation Source: UniProtKB
- protein phosphorylation Source: Ensembl
- regulation of cell growth Source: Ensembl
- regulation of glucose metabolic process Source: Ensembl
- regulation of insulin-like growth factor receptor signaling pathway Source: GO_Central
- response to insulin Source: AgBase
- type B pancreatic cell proliferation Source: Ensembl
Keywordsi
Molecular function | Growth factor binding |
Enzyme and pathway databases
Reactomei | R-BTA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-BTA-8957275, Post-translational protein phosphorylation |
Protein family/group databases
MEROPSi | I31.952 |
Names & Taxonomyi
Protein namesi | Recommended name: Insulin-like growth factor-binding protein 3Short name: IBP-3 Short name: IGF-binding protein 3 Short name: IGFBP-3 |
Gene namesi | Name:IGFBP3 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:30085, IGFBP3 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular space Source: AgBase
- insulin-like growth factor ternary complex Source: Ensembl
Nucleus
- nucleus Source: Ensembl
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 27 | 1 PublicationAdd BLAST | 27 | |
ChainiPRO_0000014377 | 28 – 291 | Insulin-like growth factor-binding protein 3Add BLAST | 264 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 118 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 136 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 148 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 199 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 201 | PhosphoserineBy similarity | 1 | |
Disulfide bondi | 213 ↔ 240 | PROSITE-ProRule annotation | ||
Disulfide bondi | 251 ↔ 262 | PROSITE-ProRule annotation | ||
Disulfide bondi | 264 ↔ 285 | PROSITE-ProRule annotation |
Post-translational modificationi
Phosphorylated by FAM20C in the extracellular medium.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | P20959 |
PRIDEi | P20959 |
Expressioni
Tissue specificityi
Plasma; expressed by most tissues.
Gene expression databases
Bgeei | ENSBTAG00000003994, Expressed in placenta and 19 other tissues |
ExpressionAtlasi | P20959, baseline and differential |
Interactioni
Subunit structurei
Interacts with HN.
Interacts with TMEM219 (By similarity). Binds IGF2 more than IGF1.
Forms a ternary complex of about 140 to 150 kDa with IGF1 or IGF2 and a 85 kDa glycoprotein (ALS).
Interacts with XLKD1.
By similarity1 PublicationGO - Molecular functioni
- fibronectin binding Source: GO_Central
- insulin-like growth factor I binding Source: GO_Central
- insulin-like growth factor II binding Source: GO_Central
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000047769 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 119 | IGFBP N-terminalPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 210 – 285 | Thyroglobulin type-1PROSITE-ProRule annotationAdd BLAST | 76 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 28 – 134 | IGF-bindingSequence analysisAdd BLAST | 107 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 138 – 161 | Ser/Thr-richAdd BLAST | 24 | |
Compositional biasi | 192 – 208 | Ser/Thr-richAdd BLAST | 17 |
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502QWC0, Eukaryota |
GeneTreei | ENSGT00940000158092 |
HOGENOMi | CLU_070833_1_1_1 |
InParanoidi | P20959 |
OMAi | PLPGYDT |
OrthoDBi | 979270at2759 |
TreeFami | TF331211 |
Family and domain databases
CDDi | cd00191, TY, 1 hit |
Gene3Di | 4.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR009030, Growth_fac_rcpt_cys_sf IPR012211, IGFBP-3 IPR000867, IGFBP-like IPR022321, IGFBP_1-6_chordata IPR017891, Insulin_GF-bd_Cys-rich_CS IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
PANTHERi | PTHR11551:SF3, PTHR11551:SF3, 1 hit |
Pfami | View protein in Pfam PF00219, IGFBP, 1 hit PF00086, Thyroglobulin_1, 1 hit |
PRINTSi | PR01976, IGFBPFAMILY PR01979, IGFBPFAMILY3 |
SMARTi | View protein in SMART SM00121, IB, 1 hit SM00211, TY, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit SSF57610, SSF57610, 1 hit |
PROSITEi | View protein in PROSITE PS00222, IGFBP_N_1, 1 hit PS51323, IGFBP_N_2, 1 hit PS00484, THYROGLOBULIN_1_1, 1 hit PS51162, THYROGLOBULIN_1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P20959-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRARPALWA AALTALTLLR GPPAARAGAG TMGAGPVVRC EPCDARAVAQ
60 70 80 90 100
CAPPPPSPPC AELVREPGCG CCLTCALREG QPCGVYTERC GSGLRCQPPP
110 120 130 140 150
GDPRPLQALL DGRGLCANAS AVGRLRPYLL PSASGNGSES EEDHSMGSTE
160 170 180 190 200
NQAGPSTHRV PVSKFHPIHT KMDVIKKGHA KDSQRYKVDY ESQSTDTQNF
210 220 230 240 250
SSESKRETEY GPCRREMEDT LNHLKFLNML SPRGIHIPNC DKKGFYKKKQ
260 270 280 290
CRPSKGRKRG FCWCVDKYGQ PLPGFDVKGK GDVHCYSMES K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 – 7 | RPA → PPR in AAA30582 (PubMed:1711841).Curated | 3 | |
Sequence conflicti | 66 – 67 | EP → DA in AAA30582 (PubMed:1711841).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76478 mRNA Translation: AAA30582.1 AF305199 mRNA Translation: AAG29824.1 AF305712 Genomic DNA Translation: AAG29825.1 BC149336 mRNA Translation: AAI49337.1 U83465 Genomic DNA Translation: AAB41430.1 |
PIRi | JN0064 |
RefSeqi | NP_776981.1, NM_174556.1 |
Genome annotation databases
Ensembli | ENSBTAT00000053426; ENSBTAP00000047769; ENSBTAG00000003994 |
GeneIDi | 282261 |
KEGGi | bta:282261 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M76478 mRNA Translation: AAA30582.1 AF305199 mRNA Translation: AAG29824.1 AF305712 Genomic DNA Translation: AAG29825.1 BC149336 mRNA Translation: AAI49337.1 U83465 Genomic DNA Translation: AAB41430.1 |
PIRi | JN0064 |
RefSeqi | NP_776981.1, NM_174556.1 |
3D structure databases
SMRi | P20959 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000047769 |
Protein family/group databases
MEROPSi | I31.952 |
Proteomic databases
PaxDbi | P20959 |
PRIDEi | P20959 |
Genome annotation databases
Ensembli | ENSBTAT00000053426; ENSBTAP00000047769; ENSBTAG00000003994 |
GeneIDi | 282261 |
KEGGi | bta:282261 |
Organism-specific databases
CTDi | 3486 |
VGNCi | VGNC:30085, IGFBP3 |
Phylogenomic databases
eggNOGi | ENOG502QWC0, Eukaryota |
GeneTreei | ENSGT00940000158092 |
HOGENOMi | CLU_070833_1_1_1 |
InParanoidi | P20959 |
OMAi | PLPGYDT |
OrthoDBi | 979270at2759 |
TreeFami | TF331211 |
Enzyme and pathway databases
Reactomei | R-BTA-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-BTA-8957275, Post-translational protein phosphorylation |
Gene expression databases
Bgeei | ENSBTAG00000003994, Expressed in placenta and 19 other tissues |
ExpressionAtlasi | P20959, baseline and differential |
Family and domain databases
CDDi | cd00191, TY, 1 hit |
Gene3Di | 4.10.800.10, 1 hit |
InterProi | View protein in InterPro IPR009030, Growth_fac_rcpt_cys_sf IPR012211, IGFBP-3 IPR000867, IGFBP-like IPR022321, IGFBP_1-6_chordata IPR017891, Insulin_GF-bd_Cys-rich_CS IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
PANTHERi | PTHR11551:SF3, PTHR11551:SF3, 1 hit |
Pfami | View protein in Pfam PF00219, IGFBP, 1 hit PF00086, Thyroglobulin_1, 1 hit |
PRINTSi | PR01976, IGFBPFAMILY PR01979, IGFBPFAMILY3 |
SMARTi | View protein in SMART SM00121, IB, 1 hit SM00211, TY, 1 hit |
SUPFAMi | SSF57184, SSF57184, 1 hit SSF57610, SSF57610, 1 hit |
PROSITEi | View protein in PROSITE PS00222, IGFBP_N_1, 1 hit PS51323, IGFBP_N_2, 1 hit PS00484, THYROGLOBULIN_1_1, 1 hit PS51162, THYROGLOBULIN_1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IBP3_BOVIN | |
Accessioni | P20959Primary (citable) accession number: P20959 Secondary accession number(s): A6QPI3, Q9GJV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 7, 2006 | |
Last modified: | February 10, 2021 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |