UniProtKB - P20917 (MAG_MOUSE)
Myelin-associated glycoprotein
Mag
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 118 | Ganglioside GT1b1 Publication | 1 |
GO - Molecular functioni
- carbohydrate binding Source: UniProtKB-KW
- ganglioside GT1b binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- sialic acid binding Source: UniProtKB
- signaling receptor binding Source: MGI
GO - Biological processi
- axon regeneration Source: MGI
- cell adhesion Source: UniProtKB
- cell-cell adhesion via plasma-membrane adhesion molecules Source: UniProtKB
- cellular response to mechanical stimulus Source: Ensembl
- central nervous system myelination Source: UniProtKB
- negative regulation of axon extension Source: UniProtKB
- negative regulation of neuron apoptotic process Source: UniProtKB
- negative regulation of neuron differentiation Source: MGI
- negative regulation of neuron projection development Source: UniProtKB
- positive regulation of astrocyte differentiation Source: MGI
- positive regulation of myelination Source: CACAO
- transmission of nerve impulse Source: MGI
Keywordsi
Biological process | Cell adhesion |
Ligand | Lectin, Lipid-binding |
Enzyme and pathway databases
Reactomei | R-MMU-193634, Axonal growth inhibition (RHOA activation) R-MMU-210991, Basigin interactions |
Protein family/group databases
UniLectini | P20917 |
Names & Taxonomyi
Protein namesi | Recommended name: Myelin-associated glycoproteinAlternative name(s): Siglec-4a |
Gene namesi | Name:Mag |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96912, Mag |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Single-pass type I membrane protein 2 Publications
Other locations
- Membrane raft By similarity
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- plasma membrane Source: UniProtKB
Other locations
- compact myelin Source: MGI
- cytoplasm Source: MGI
- membrane raft Source: UniProtKB-SubCell
- mesaxon Source: MGI
- myelin sheath Source: MGI
- myelin sheath adaxonal region Source: MGI
- paranode region of axon Source: BHF-UCL
- Schmidt-Lanterman incisure Source: BHF-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 20 – 516 | ExtracellularSequence analysisAdd BLAST | 497 | |
Transmembranei | 517 – 536 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 537 – 627 | CytoplasmicSequence analysisAdd BLAST | 91 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 25 | W → Q: Abolishes C-linked mannosylation. 1 Publication | 1 | |
Mutagenesisi | 65 | Y → A: Decreases ganglioside binding. 1 Publication | 1 | |
Mutagenesisi | 118 – 120 | RGD → KGE: Abolishes protection against axon degeneration. 1 Publication | 3 | |
Mutagenesisi | 118 | R → A: Abolishes ganglioside binding. 1 Publication | 1 | |
Mutagenesisi | 127 | Y → A: Abolishes ganglioside binding. 1 Publication | 1 | |
Mutagenesisi | 128 | T → A: Abolishes ganglioside binding. 1 Publication | 1 | |
Mutagenesisi | 406 | N → Q: Increases homodimerization. 1 Publication | 1 | |
Mutagenesisi | 473 | I → E: Abolishes homodimerization. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1250416 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Add BLAST | 19 | |
ChainiPRO_0000014857 | 20 – 627 | Myelin-associated glycoproteinAdd BLAST | 608 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 22 | C-linked (Man) tryptophan1 Publication | 1 | |
Disulfide bondi | 37 ↔ 165 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 42 ↔ 100 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 99 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 159 ↔ 217 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 223 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 246 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 261 ↔ 305 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 315 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 332 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 347 ↔ 392 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 406 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 421 ↔ 430 | PROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 432 ↔ 488 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 450 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 454 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Lipidationi | 531 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 545 | PhosphoserineBy similarity | 1 | |
Modified residuei | 547 | PhosphoserineBy similarity | 1 | |
Modified residuei | 549 | PhosphoserineBy similarity | 1 | |
Modified residuei | 591 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | P20917 |
PaxDbi | P20917 |
PeptideAtlasi | P20917 |
PRIDEi | P20917 |
PTM databases
GlyConnecti | 2446, 2 N-Linked glycans (1 site) [P20917-2] 2519, 13 N-Linked glycans (1 site) |
GlyGeni | P20917, 9 sites |
iPTMneti | P20917 |
PhosphoSitePlusi | P20917 |
SwissPalmi | P20917 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000036634, Expressed in ventral horn of spinal cord and 152 other tissues |
Genevisiblei | P20917, MM |
Interactioni
Subunit structurei
GO - Molecular functioni
- protein homodimerization activity Source: UniProtKB
- protein kinase binding Source: MGI
- signaling receptor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 201285, 5 interactors |
IntActi | P20917, 4 interactors |
MINTi | P20917 |
STRINGi | 10090.ENSMUSP00000139881 |
Miscellaneous databases
RNActi | P20917, protein |
Structurei
Secondary structure
3D structure databases
SASBDBi | P20917 |
SMRi | P20917 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 120 | Ig-like V-typeAdd BLAST | 99 | |
Domaini | 139 – 237 | Ig-like C2-type 1Add BLAST | 99 | |
Domaini | 241 – 325 | Ig-like C2-type 2Add BLAST | 85 | |
Domaini | 327 – 412 | Ig-like C2-type 3Add BLAST | 86 | |
Domaini | 413 – 508 | Ig-like C2-type 4Add BLAST | 96 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 325 | Interaction with RTN4R and RTN4RL2By similarityAdd BLAST | 306 | |
Regioni | 65 – 67 | Ganglioside GT1b binding1 Publication | 3 | |
Regioni | 124 – 128 | Ganglioside GT1b binding1 Publication | 5 | |
Regioni | 578 – 627 | Required for normal axon myelination in the central nervous system1 PublicationAdd BLAST | 50 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4475, Eukaryota |
GeneTreei | ENSGT01010000222452 |
HOGENOMi | CLU_020480_1_0_1 |
InParanoidi | P20917 |
OMAi | CVVKANP |
OrthoDBi | 415025at2759 |
PhylomeDBi | P20917 |
TreeFami | TF332441 |
Family and domain databases
Gene3Di | 2.60.40.10, 4 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF08205, C2-set_2, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 4 hits SM00408, IGc2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MIFLATLPLF WIMISASRGG HWGAWMPSTI SAFEGTCVSI PCRFDFPDEL
60 70 80 90 100
RPAVVHGVWY FNSPYPKNYP PVVFKSRTQV VHESFQGRSR LLGDLGLRNC
110 120 130 140 150
TLLLSTLSPE LGGKYYFRGD LGGYNQYTFS EHSVLDIVNT PNIVVPPEVV
160 170 180 190 200
AGTEVEVSCM VPDNCPELRP ELSWLGHEGL GEPTVLGRLR EDEGTWVQVS
210 220 230 240 250
LLHFVPTREA NGHRLGCQAA FPNTTLQFEG YASLDVKYPP VIVEMNSSVE
260 270 280 290 300
AIEGSHVSLL CGADSNPPPL LTWMRDGMVL REAVAKSLYL DLEEVTPGED
310 320 330 340 350
GVYACLAENA YGQDNRTVEL SVMYAPWKPT VNGTVVAVEG ETVSILCSTQ
360 370 380 390 400
SNPDPILTIF KEKQILATVI YESQLQLELP AVTPEDDGEY WCVAENQYGQ
410 420 430 440 450
RATAFNLSVE FAPIILLESH CAAARDTVQC LCVVKSNPEP SVAFELPSRN
460 470 480 490 500
VTVNETEREF VYSERSGLLL TSILTIRGQA QAPPRVICTS RNLYGTQSLE
510 520 530 540 550
LPFQGAHRLM WAKIGPVGAV VAFAILIAIV CYITQTRRKK NVTESSSFSG
560 570 580 590 600
GDNPHVLYSP EFRISGAPDK YESEKQRLGS ERRLLGLRGE SPELDLSYSH
610 620
SDLGKRPTKD SYTLTEELAE YAEIRVK
The sequence of this isoform differs from the canonical sequence as follows:
574-582: EKQRLGSER → REVSTRDCH
583-627: Missing.
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3ZB60 | Q3ZB60_MOUSE | Mag protein | Mag | 567 | Annotation score: | ||
A0A087WRD6 | A0A087WRD6_MOUSE | Myelin-associated glycoprotein | Mag | 168 | Annotation score: | ||
A0A087WPP5 | A0A087WPP5_MOUSE | Myelin-associated glycoprotein | Mag | 64 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002527 | 574 – 582 | EKQRLGSER → REVSTRDCH in isoform S-MAG. Curated | 9 | |
Alternative sequenceiVSP_002528 | 583 – 627 | Missing in isoform S-MAG. CuratedAdd BLAST | 45 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31811 mRNA Translation: AAA39487.1 M74793 , M74783, M74784, M74785, M74786, M74787, M74788, M74790, M74791 Genomic DNA Translation: AAA91743.1 AC165340 Genomic DNA No translation available. |
CCDSi | CCDS21115.1 [P20917-2] CCDS90207.1 [P20917-1] |
PIRi | B33785 |
RefSeqi | NP_001333013.1, NM_001346084.1 [P20917-1] NP_001333014.1, NM_001346085.1 [P20917-1] NP_001333015.1, NM_001346086.1 [P20917-2] NP_001333016.1, NM_001346087.1 [P20917-2] NP_001333017.1, NM_001346088.1 [P20917-2] NP_034888.1, NM_010758.3 [P20917-2] XP_006539653.1, XM_006539590.2 XP_006539654.1, XM_006539591.3 XP_011248740.1, XM_011250438.2 XP_011248741.1, XM_011250439.2 [P20917-1] XP_011248742.1, XM_011250440.2 [P20917-1] XP_011248743.1, XM_011250441.2 XP_017177496.1, XM_017322007.1 [P20917-1] XP_017177497.1, XM_017322008.1 |
Genome annotation databases
Ensembli | ENSMUST00000040548; ENSMUSP00000041464; ENSMUSG00000036634 [P20917-2] ENSMUST00000187137; ENSMUSP00000139564; ENSMUSG00000036634 [P20917-2] ENSMUST00000191081; ENSMUSP00000139881; ENSMUSG00000036634 [P20917-1] |
GeneIDi | 17136 |
KEGGi | mmu:17136 |
UCSCi | uc009ghb.1, mouse [P20917-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - Glycan Binding Siglec-4 |
Functional Glycomics Gateway - Glycan Binding Siglec-4a [3 Fc Domains] |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31811 mRNA Translation: AAA39487.1 M74793 , M74783, M74784, M74785, M74786, M74787, M74788, M74790, M74791 Genomic DNA Translation: AAA91743.1 AC165340 Genomic DNA No translation available. |
CCDSi | CCDS21115.1 [P20917-2] CCDS90207.1 [P20917-1] |
PIRi | B33785 |
RefSeqi | NP_001333013.1, NM_001346084.1 [P20917-1] NP_001333014.1, NM_001346085.1 [P20917-1] NP_001333015.1, NM_001346086.1 [P20917-2] NP_001333016.1, NM_001346087.1 [P20917-2] NP_001333017.1, NM_001346088.1 [P20917-2] NP_034888.1, NM_010758.3 [P20917-2] XP_006539653.1, XM_006539590.2 XP_006539654.1, XM_006539591.3 XP_011248740.1, XM_011250438.2 XP_011248741.1, XM_011250439.2 [P20917-1] XP_011248742.1, XM_011250440.2 [P20917-1] XP_011248743.1, XM_011250441.2 XP_017177496.1, XM_017322007.1 [P20917-1] XP_017177497.1, XM_017322008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5LF5 | X-ray | 3.80 | A | 20-508 | [»] | |
5LFR | X-ray | 2.12 | A/B | 20-325 | [»] | |
5LFU | X-ray | 4.30 | A | 20-508 | [»] | |
5LFV | X-ray | 2.30 | A/B | 20-325 | [»] | |
6GZJ | X-ray | 1.98 | B | 573-627 | [»] | |
6GZL | X-ray | 1.95 | B | 605-621 | [»] | |
SASBDBi | P20917 | |||||
SMRi | P20917 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 201285, 5 interactors |
IntActi | P20917, 4 interactors |
MINTi | P20917 |
STRINGi | 10090.ENSMUSP00000139881 |
Chemistry databases
ChEMBLi | CHEMBL1250416 |
Protein family/group databases
UniLectini | P20917 |
PTM databases
GlyConnecti | 2446, 2 N-Linked glycans (1 site) [P20917-2] 2519, 13 N-Linked glycans (1 site) |
GlyGeni | P20917, 9 sites |
iPTMneti | P20917 |
PhosphoSitePlusi | P20917 |
SwissPalmi | P20917 |
Proteomic databases
MaxQBi | P20917 |
PaxDbi | P20917 |
PeptideAtlasi | P20917 |
PRIDEi | P20917 |
Protocols and materials databases
ABCDi | P20917, 19 sequenced antibodies |
Antibodypediai | 1364, 382 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000040548; ENSMUSP00000041464; ENSMUSG00000036634 [P20917-2] ENSMUST00000187137; ENSMUSP00000139564; ENSMUSG00000036634 [P20917-2] ENSMUST00000191081; ENSMUSP00000139881; ENSMUSG00000036634 [P20917-1] |
GeneIDi | 17136 |
KEGGi | mmu:17136 |
UCSCi | uc009ghb.1, mouse [P20917-2] |
Organism-specific databases
CTDi | 4099 |
MGIi | MGI:96912, Mag |
Phylogenomic databases
eggNOGi | KOG4475, Eukaryota |
GeneTreei | ENSGT01010000222452 |
HOGENOMi | CLU_020480_1_0_1 |
InParanoidi | P20917 |
OMAi | CVVKANP |
OrthoDBi | 415025at2759 |
PhylomeDBi | P20917 |
TreeFami | TF332441 |
Enzyme and pathway databases
Reactomei | R-MMU-193634, Axonal growth inhibition (RHOA activation) R-MMU-210991, Basigin interactions |
Miscellaneous databases
BioGRID-ORCSi | 17136, 0 hits in 17 CRISPR screens |
PROi | PR:P20917 |
RNActi | P20917, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036634, Expressed in ventral horn of spinal cord and 152 other tissues |
Genevisiblei | P20917, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 4 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR003598, Ig_sub2 |
Pfami | View protein in Pfam PF08205, C2-set_2, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 4 hits SM00408, IGc2, 2 hits |
SUPFAMi | SSF48726, SSF48726, 4 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAG_MOUSE | |
Accessioni | P20917Primary (citable) accession number: P20917 Secondary accession number(s): A0A087WPR1, P16880 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | September 18, 2019 | |
Last modified: | December 2, 2020 | |
This is version 194 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families