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Protein

Cytochrome P450 2A5

Gene

Cyp2a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Exhibits a high coumarin 7-hydroxylase activity.

Miscellaneous

There are only 11 differences between the sequence of testosterone 15-alpha-hydroxylase and that of coumarin 7-hydroxylase. By site-directed mutagenesis it has been shown that modification of position 209 is sufficient to convert the specificity of the two forms of the enzyme.

Catalytic activityi

RH + [reduced NADPH--hemoprotein reductase] + O2 = ROH + [oxidized NADPH--hemoprotein reductase] + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi439Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKiP20852

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2A5 (EC:1.14.14.1)
Alternative name(s):
CYPIIA5
Coumarin 7-hydroxylase
Cytochrome P450-15-COH
Cytochrome P450-IIA3.2
Gene namesi
Name:Cyp2a5
Synonyms:Cyp2a-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88597 Cyp2a5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4085

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516671 – 494Cytochrome P450 2A5Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphoserineBy similarity1
Modified residuei379N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP20852
PaxDbiP20852
PeptideAtlasiP20852
PRIDEiP20852

PTM databases

iPTMnetiP20852
PhosphoSitePlusiP20852

Expressioni

Tissue specificityi

Liver, with a strong circadian rhythmicity. Circadian expression is regulated by DBP.1 Publication

Developmental stagei

In liver; activity 6 fold higher in females than in males.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005685

Chemistry databases

BindingDBiP20852

Structurei

3D structure databases

ProteinModelPortaliP20852
SMRiP20852
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOGENOMiHOG000036992
HOVERGENiHBG015789
InParanoidiP20852
PhylomeDBiP20852

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008067 Cyt_P450_E_grp-I_CYP2A-like
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01684 EP450ICYP2A
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

P20852-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTSGLLLVA AVAFLSVLVL MSVWKQRKLS GKLPPGPTPL PFIGNFLQLN
60 70 80 90 100
TEQMYNSLMK ISQRYGPVFT IYLGPRRIVV LCGQEAVKEA LVDQAEEFSG
110 120 130 140 150
RGEQATFDWL FKGYGVVFSS GERAKQLRRF SIATLRDFGV GKRGIEERIQ
160 170 180 190 200
EEAGFLIDSF RKTNGAFIDP TFYLSRTVSN VISSIVFGDR FDYEDKEFLS
210 220 230 240 250
LLRMMLGSFQ FTATSMGQLY EMFSSVMKHL PGPQQQAFKE LQGLEDFITK
260 270 280 290 300
KVEHNQRTLD PNSPRDFIDS FLIRMLEEKK NPNTEFYMKN LVLTTLNLFF
310 320 330 340 350
AGTETVSTTL RYGFLLLMKH PDIEAKVHEE IDRVIGRNRQ PKYEDRMKMP
360 370 380 390 400
YTEAVIHEIQ RFADMIPMGL ARRVTKDTKF RDFLLPKGTE VFPMLGSVLK
410 420 430 440 450
DPKFFSNPKD FNPKHFLDDK GQFKKNDAFV PFSIGKRYCF GEGLARMELF
460 470 480 490
LFLTNIMQNF HFKSTQAPQD IDVSPRLVGF ATIPPTYTMS FLSR
Length:494
Mass (Da):56,741
Last modified:February 1, 1991 - v1
Checksum:i1C2516D5FA2551D0
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q91X75Q91X75_MOUSE
Cyp2a4 protein
Cyp2a5
494Annotation score:
E9Q5D4E9Q5D4_MOUSE
Cytochrome P450 2A5
Cyp2a5
61Annotation score:
F7B9W9F7B9W9_MOUSE
Cytochrome P450 2A5
Cyp2a5
128Annotation score:
F6SHL3F6SHL3_MOUSE
Cytochrome P450 2A5
Cyp2a5
72Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25211
, M26204, M25205, M25206, M25207, M25208, M25209, M25210 Genomic DNA Translation: AAA37798.1
X89864 mRNA Translation: CAA61963.1
CCDSiCCDS21008.1
PIRiB33531
UniGeneiMm.389848

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25211
, M26204, M25205, M25206, M25207, M25208, M25209, M25210 Genomic DNA Translation: AAA37798.1
X89864 mRNA Translation: CAA61963.1
CCDSiCCDS21008.1
PIRiB33531
UniGeneiMm.389848

3D structure databases

ProteinModelPortaliP20852
SMRiP20852
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005685

Chemistry databases

BindingDBiP20852
ChEMBLiCHEMBL4085

PTM databases

iPTMnetiP20852
PhosphoSitePlusiP20852

Proteomic databases

MaxQBiP20852
PaxDbiP20852
PeptideAtlasiP20852
PRIDEiP20852

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:88597 Cyp2a5

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOGENOMiHOG000036992
HOVERGENiHBG015789
InParanoidiP20852
PhylomeDBiP20852

Enzyme and pathway databases

SABIO-RKiP20852

Miscellaneous databases

PROiPR:P20852
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR008067 Cyt_P450_E_grp-I_CYP2A-like
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR01684 EP450ICYP2A
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCP2A5_MOUSE
AccessioniPrimary (citable) accession number: P20852
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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