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Protein

Kit ligand

Gene

Kitlg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.

GO - Molecular functioni

  • cytokine activity Source: MGI
  • growth factor activity Source: UniProtKB-KW
  • stem cell factor receptor binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionGrowth factor
Biological processCell adhesion

Enzyme and pathway databases

ReactomeiR-MMU-1257604 PIP3 activates AKT signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-1433559 Regulation of KIT signaling
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Kit ligand
Alternative name(s):
Hematopoietic growth factor KL
Mast cell growth factor
Short name:
MGF
Steel factor
Stem cell factor
Short name:
SCF
c-Kit ligand
Cleaved into the following chain:
Soluble KIT ligand
Short name:
sKITLG
Gene namesi
Name:Kitlg
Synonyms:Kitl, Mgf, Sl, Slf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96974 Kitl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 214ExtracellularSequence analysisAdd BLAST189
Transmembranei215 – 237HelicalSequence analysisAdd BLAST23
Topological domaini238 – 273CytoplasmicSequence analysisAdd BLAST36

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 253 PublicationsAdd BLAST25
ChainiPRO_000003191426 – 273Kit ligandAdd BLAST248
ChainiPRO_000040339226 – 190Soluble KIT ligandBy similarityAdd BLAST165

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi29 ↔ 1141 Publication
Disulfide bondi68 ↔ 1631 Publication
Glycosylationi90N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi97N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi195N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP20826
PaxDbiP20826
PeptideAtlasiP20826
PRIDEiP20826

PTM databases

PhosphoSitePlusiP20826

Miscellaneous databases

PMAP-CutDBiP20826

Expressioni

Tissue specificityi

Developmental stagei

Acts in the early stages of hematopoiesis.

Gene expression databases

BgeeiENSMUSG00000019966 Expressed in 372 organ(s), highest expression level in brain blood vessel
CleanExiMM_KITL
ExpressionAtlasiP20826 baseline and differential
GenevisibleiP20826 MM

Interactioni

Subunit structurei

Homodimer, non-covalently linked (Probable). Heterotetramer with KIT, binding two KIT molecules; thereby mediates KIT dimerization and subsequent activation by autophosphorylation.Curated1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201412, 1 interactor
STRINGi10090.ENSMUSP00000100920

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20826
SMRiP20826
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20826

Family & Domainsi

Sequence similaritiesi

Belongs to the SCF family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF3Y Eukaryota
ENOG410XYQR LUCA
GeneTreeiENSGT00390000018272
HOGENOMiHOG000082493
HOVERGENiHBG036146
InParanoidiP20826
KOiK05461
OMAiVKTKGIC
OrthoDBiEOG091G0I23
PhylomeDBiP20826
TreeFamiTF330811

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR003452 SCF
PANTHERiPTHR11574 PTHR11574, 1 hit
PfamiView protein in Pfam
PF02404 SCF, 1 hit
PIRSFiPIRSF015599 SCF, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P20826-1) [UniParc]FASTAAdd to basket
Also known as: KL-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKKTQTWIIT CIYLQLLLFN PLVKTKEICG NPVTDNVKDI TKLVANLPND
60 70 80 90 100
YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI
110 120 130 140 150
IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA
160 170 180 190 200
FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVAA SSLRNDSSSS
210 220 230 240 250
NRKAAKAPED SGLQWTAMAL PALISLVIGF AFGALYWKKK QSSLTRAVEN
260 270
IQINEEDNEI SMLQQKEREF QEV
Length:273
Mass (Da):30,645
Last modified:February 1, 1991 - v1
Checksum:iA7FC899B592A7967
GO
Isoform 2 (identifier: P20826-2) [UniParc]FASTAAdd to basket
Also known as: KL-2

The sequence of this isoform differs from the canonical sequence as follows:
     174-202: DSRVSVTKPFMLPPVAASSLRNDSSSSNR → G

Show »
Length:245
Mass (Da):27,614
Checksum:i78160A204FABF93B
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q8C9K1Q8C9K1_MOUSE
Kit ligand
Kitl
160Annotation score:
A0A1W2P773A0A1W2P773_MOUSE
Kit ligand
Kitl
56Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti215W → L in AAB22555 (PubMed:1378327).Curated1
Sequence conflicti215W → L in AAB22554 (PubMed:1378327).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti122N → S in MGFSL-3NEU. 1 Publication1
Natural varianti193L → P2 Publications1
Natural varianti207A → S3 Publications1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006023174 – 202DSRVS…SSSNR → G in isoform 2. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59915 mRNA Translation: AAA40095.1
M57647 mRNA Translation: AAA39538.1
S40534 mRNA Translation: AAB22555.2
X68989 mRNA Translation: CAA48778.1
U44724 Genomic DNA No translation available.
U44725 mRNA Translation: AAC52447.1
X95381 mRNA Translation: CAA64667.1
X99322 mRNA Translation: CAA67698.1
Y10287 mRNA Translation: CAA71329.1
AK018777 mRNA Translation: BAB31402.1
AK134301 mRNA Translation: BAE22091.1
AK165233 mRNA Translation: BAE38091.1
BC011322 mRNA Translation: AAH11322.1
S40364 mRNA Translation: AAB22554.2
M59912 mRNA Translation: AAA39539.1
CCDSiCCDS36046.1 [P20826-1]
CCDS83751.1 [P20826-2]
PIRiA35971
A37934
B35971
S65801
RefSeqiNP_001334085.1, NM_001347156.1 [P20826-2]
NP_038626.1, NM_013598.3 [P20826-1]
UniGeneiMm.45124

Genome annotation databases

EnsembliENSMUST00000020129; ENSMUSP00000020129; ENSMUSG00000019966 [P20826-2]
ENSMUST00000105283; ENSMUSP00000100920; ENSMUSG00000019966 [P20826-1]
GeneIDi17311
KEGGimmu:17311
UCSCiuc007gxp.1 mouse [P20826-1]
uc007gxq.1 mouse [P20826-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59915 mRNA Translation: AAA40095.1
M57647 mRNA Translation: AAA39538.1
S40534 mRNA Translation: AAB22555.2
X68989 mRNA Translation: CAA48778.1
U44724 Genomic DNA No translation available.
U44725 mRNA Translation: AAC52447.1
X95381 mRNA Translation: CAA64667.1
X99322 mRNA Translation: CAA67698.1
Y10287 mRNA Translation: CAA71329.1
AK018777 mRNA Translation: BAB31402.1
AK134301 mRNA Translation: BAE22091.1
AK165233 mRNA Translation: BAE38091.1
BC011322 mRNA Translation: AAH11322.1
S40364 mRNA Translation: AAB22554.2
M59912 mRNA Translation: AAA39539.1
CCDSiCCDS36046.1 [P20826-1]
CCDS83751.1 [P20826-2]
PIRiA35971
A37934
B35971
S65801
RefSeqiNP_001334085.1, NM_001347156.1 [P20826-2]
NP_038626.1, NM_013598.3 [P20826-1]
UniGeneiMm.45124

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2O26X-ray2.50A/B/E/F28-166[»]
2O27X-ray2.20A/B28-166[»]
ProteinModelPortaliP20826
SMRiP20826
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201412, 1 interactor
STRINGi10090.ENSMUSP00000100920

PTM databases

PhosphoSitePlusiP20826

Proteomic databases

MaxQBiP20826
PaxDbiP20826
PeptideAtlasiP20826
PRIDEiP20826

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020129; ENSMUSP00000020129; ENSMUSG00000019966 [P20826-2]
ENSMUST00000105283; ENSMUSP00000100920; ENSMUSG00000019966 [P20826-1]
GeneIDi17311
KEGGimmu:17311
UCSCiuc007gxp.1 mouse [P20826-1]
uc007gxq.1 mouse [P20826-2]

Organism-specific databases

CTDi17311
MGIiMGI:96974 Kitl

Phylogenomic databases

eggNOGiENOG410IF3Y Eukaryota
ENOG410XYQR LUCA
GeneTreeiENSGT00390000018272
HOGENOMiHOG000082493
HOVERGENiHBG036146
InParanoidiP20826
KOiK05461
OMAiVKTKGIC
OrthoDBiEOG091G0I23
PhylomeDBiP20826
TreeFamiTF330811

Enzyme and pathway databases

ReactomeiR-MMU-1257604 PIP3 activates AKT signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-1433559 Regulation of KIT signaling
R-MMU-5673001 RAF/MAP kinase cascade
R-MMU-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling

Miscellaneous databases

EvolutionaryTraceiP20826
PMAP-CutDBiP20826
PROiPR:P20826
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019966 Expressed in 372 organ(s), highest expression level in brain blood vessel
CleanExiMM_KITL
ExpressionAtlasiP20826 baseline and differential
GenevisibleiP20826 MM

Family and domain databases

InterProiView protein in InterPro
IPR009079 4_helix_cytokine-like_core
IPR003452 SCF
PANTHERiPTHR11574 PTHR11574, 1 hit
PfamiView protein in Pfam
PF02404 SCF, 1 hit
PIRSFiPIRSF015599 SCF, 1 hit
SUPFAMiSSF47266 SSF47266, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSCF_MOUSE
AccessioniPrimary (citable) accession number: P20826
Secondary accession number(s): P97332
, Q3TNJ7, Q62524, Q64222, Q921N5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 174 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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