Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

UniProtKB - P20810 (ICAL_HUMAN)

(max 400 entries)x

Your basket is currently empty. i

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Calpastatin

Gene

CAST

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • calcium-dependent cysteine-type endopeptidase inhibitor activity Source: CAFA
  • endopeptidase inhibitor activity Source: ProtInc
  • RNA binding Source: UniProtKB
View the complete GO annotation on QuickGO ...

GO - Biological processi

  • inhibition of cysteine-type endopeptidase activity Source: CAFA
  • negative regulation of type B pancreatic cell apoptotic process Source: BHF-UCL
  • presynaptic active zone organization Source: ARUK-UCL
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionProtease inhibitor, Thiol protease inhibitor

Enzyme and pathway databases

ReactomeiR-HSA-1474228 Degradation of the extracellular matrix
R-HSA-8862803 Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models
SIGNORiP20810

Protein family/group databases

MEROPSiI27.001

Names & Taxonomyi

Protein namesi
Recommended name:
Calpastatin
Alternative name(s):
Calpain inhibitor
Sperm BS-17 component
Gene namesi
Name:CAST
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000153113.23
HGNCiHGNC:1515 CAST
MIMi114090 gene
neXtProtiNX_P20810

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Involvement in diseasei

Peeling skin with leukonychia, acral punctate keratoses, cheilitis, and knuckle pads (PLACK)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive disease characterized by generalized, continuous shedding of the outer layers of the epidermis, leukonychia, acral punctate keratosis, cheilitis, knuckle pads with multiple hyperkeratotic micropapules involving the interphalangeal joints, and palmoplantar keratoderma.
See also OMIM:616295

Keywords - Diseasei

Palmoplantar keratoderma

Organism-specific databases

DisGeNETi831
MalaCardsiCAST
MIMi616295 phenotype
OpenTargetsiENSG00000153113
PharmGKBiPA26098

Chemistry databases

DrugBankiDB01373 Calcium

Polymorphism and mutation databases

BioMutaiCAST
DMDMi126302556

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001476321 – 708CalpastatinAdd BLAST708

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki32Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei133PhosphoserineCombined sources1
Modified residuei135PhosphothreonineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei243PhosphoserineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei366PhosphoserineCombined sources1
Modified residuei373PhosphoserineCombined sources1
Modified residuei440PhosphoserineCombined sources1
Modified residuei516PhosphoserineCombined sources1
Modified residuei527PhosphoserineCombined sources1
Modified residuei563PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei577PhosphoserineCombined sources1
Isoform 5 (identifier: P20810-5)
Modified residuei11PhosphoserineCombined sources1
Isoform 6 (identifier: P20810-6)
Modified residuei11PhosphoserineCombined sources1
Isoform 7 (identifier: P20810-7)
Modified residuei11PhosphoserineCombined sources1
Isoform 9 (identifier: P20810-9)
Modified residuei11PhosphoserineCombined sources1
Isoform 10 (identifier: P20810-10)
Modified residuei11PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP20810
MaxQBiP20810
PeptideAtlasiP20810
PRIDEiP20810
ProteomicsDBi53796
53797 [P20810-2]
53798 [P20810-3]
53799 [P20810-4]
53800 [P20810-5]
53801 [P20810-6]
53802 [P20810-7]
TopDownProteomicsiP20810-5 [P20810-5]

PTM databases

iPTMnetiP20810
PhosphoSitePlusiP20810

Expressioni

Gene expression databases

BgeeiENSG00000153113
CleanExiHS_CAST
ExpressionAtlasiP20810 baseline and differential
GenevisibleiP20810 HS

Organism-specific databases

HPAiCAB009491
HPA036881
HPA036882

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridi107281, 33 interactors
IntActiP20810, 15 interactors
MINTiP20810

Structurei

3D structure databases

DisProtiDP00196
ProteinModelPortaliP20810
SMRiP20810
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati170 – 222Inhibitory domain 1Add BLAST53
Repeati304 – 356Inhibitory domain 2Add BLAST53
Repeati446 – 499Inhibitory domain 3Add BLAST54
Repeati583 – 636Inhibitory domain 4Add BLAST54

Domaini

Each of the four flexible inhibitory domains can inhibit one calcium-bound calpain molecule by occupying both sides of the active site.By similarity

Sequence similaritiesi

Belongs to the protease inhibitor I27 (calpastatin) family. [View classification]Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000002993
HOGENOMiHOG000082405
HOVERGENiHBG000183
InParanoidiP20810
KOiK04281
OMAiKSSSMNP
OrthoDBiEOG091G0553
PhylomeDBiP20810
TreeFamiTF332525

Family and domain databases

InterProiView protein in InterPro
IPR026998 Calpastatin
IPR001259 Prot_inh_calpain
PANTHERiPTHR10077 PTHR10077, 1 hit
PfamiView protein in Pfam
PF00748 Calpain_inhib, 4 hits

Sequences (10)i

Sequence statusi: Complete.

This entry describes 10 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P20810-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPTETKAIP VSQQMEGPHL PNKKKHKKQA VKTEPEKKSQ STKLSVVHEK 
        60         70         80         90        100
KSQEGKPKEH TEPKSLPKQA SDTGSNDAHN KKAVSRSAEQ QPSEKSTEPK 
       110        120        130        140        150
TKPQDMISAG GESVAGITAI SGKPGDKKKE KKSLTPAVPV ESKPDKPSGK 
       160        170        180        190        200
SGMDAALDDL IDTLGGPEET EEENTTYTGP EVSDPMSSTY IEELGKREVT 
       210        220        230        240        250
IPPKYRELLA KKEGITGPPA DSSKPIGPDD AIDALSSDFT CGSPTAAGKK 
       260        270        280        290        300
TEKEESTEVL KAQSAGTVRS AAPPQEKKRK VEKDTMSDQA LEALSASLGT 
       310        320        330        340        350
RQAEPELDLR SIKEVDEAKA KEEKLEKCGE DDETIPSEYR LKPATDKDGK 
       360        370        380        390        400
PLLPEPEEKP KPRSESELID ELSEDFDRSE CKEKPSKPTE KTEESKAAAP 
       410        420        430        440        450
APVSEAVCRT SMCSIQSAPP EPATLKGTVP DDAVEALADS LGKKEADPED 
       460        470        480        490        500
GKPVMDKVKE KAKEEDREKL GEKEETIPPD YRLEEVKDKD GKPLLPKESK 
       510        520        530        540        550
EQLPPMSEDF LLDALSEDFS GPQNASSLKF EDAKLAAAIS EVVSQTPAST 
       560        570        580        590        600
TQAGAPPRDT SQSDKDLDDA LDKLSDSLGQ RQPDPDENKP MEDKVKEKAK 
       610        620        630        640        650
AEHRDKLGER DDTIPPEYRH LLDDNGQDKP VKPPTKKSED SKKPADDQDP 
       660        670        680        690        700
IDALSGDLDS CPSTTETSQN TAKDKCKKAA SSSKAPKNGG KAKDSAKTTE 

ETSKPKDD
Length:708
Mass (Da):76,573
Last modified:February 20, 2007 - v4
Checksum:i2DB0C14B860E89C7
GO
Isoform 2 (identifier: P20810-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-224: Missing.

Show »
Length:695
Mass (Da):75,304
Checksum:i9DBFF022F785D117
GO
Isoform 3 (identifier: P20810-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-105: Missing.
     212-224: Missing.

Show »
Length:590
Mass (Da):63,665
Checksum:iDB776EDB8EC13005
GO
Isoform 4 (identifier: P20810-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-30: Missing.
     44-62: Missing.

Show »
Length:667
Mass (Da):71,894
Checksum:i47831647A417E024
GO
Isoform 5 (identifier: P20810-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MNPTETKAIPVSQQMEGPHLPNKKKHKKQ → MSQPGQKPAA...SSSMNPTETK
     212-224: Missing.

Show »
Length:756
Mass (Da):81,169
Checksum:i18BA3EAB0A9989D3
GO
Isoform 6 (identifier: P20810-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSQPGQKPAA...SGATSKSSSM

Show »
Length:791
Mass (Da):84,943
Checksum:iDC3DC0A4FC0F0825
GO
Isoform 7 (identifier: P20810-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSQPGQKPAA...SGATSKSSSM
     44-62: Missing.

Show »
Length:772
Mass (Da):82,771
Checksum:i1B147E0CE4CD15E9
GO
Isoform 8 (identifier: P20810-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-30: Missing.

Note: No experimental confirmation available.
Show »
Length:686
Mass (Da):74,067
Checksum:i3B383857507798D3
GO
Isoform 9 (identifier: P20810-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: MNPTETKAIP...QEGKPKEHTE → MSQPGQKPAA...EPEKKSQSTK

Note: Gene prediction based on EST data.Combined sources
Show »
Length:750
Mass (Da):80,265
Checksum:i41EF9956565188AD
GO
Isoform 10 (identifier: P20810-10) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MNPTETKAIPVSQQMEGPHLPNKKKHKKQ → MSQPGQKPAA...SSSMNPTETK

Note: No experimental confirmation available.Combined sources
Show »
Length:769
Mass (Da):82,437
Checksum:iB29C93684805FE0B
GO

Sequence cautioni

The sequence AAH16066 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti467R → L in AAC50136 (Ref. 12) Curated1
Sequence conflicti486 – 488VKD → GKE in AAC50136 (Ref. 12) Curated3
Sequence conflicti543V → L in AAC50136 (Ref. 12) Curated1
Sequence conflicti562Missing in AAB60371 (Ref. 15) Curated1
Sequence conflicti651I → V in AAD09102 (Ref. 16) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_030741380E → K. Corresponds to variant dbSNP:rs1643702Ensembl.1
Natural variantiVAR_022686408C → S4 PublicationsCorresponds to variant dbSNP:rs754615Ensembl.1
Natural variantiVAR_030742537A → V. Corresponds to variant dbSNP:rs4948Ensembl.1
Natural variantiVAR_005298592E → G1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0007411 – 105Missing in isoform 3. 1 PublicationAdd BLAST105
Alternative sequenceiVSP_0473931 – 62MNPTE…KEHTE → MSQPGQKPAASPRPRRAAAA RRTHEHVSEKTSESPSKPGE KKGSDEKKAASLGSSQSSRT YAGGTASATKVSASSGATSK SSSMNPTETKAVKTEPEKKS QSTK in isoform 9. CuratedAdd BLAST62
Alternative sequenceiVSP_0380371 – 29MNPTE…KHKKQ → MSQPGQKPAASPRPRRAAAA RRTHEHVSEKTSESPSKPGE KKGSDEKKAASLGSSQSSRT YAGGTASATKVSASSGATSK SSSMNPTETK in isoform 5 and isoform 10. 2 PublicationsAdd BLAST29
Alternative sequenceiVSP_0380381M → MSQPGQKPAASPRPRRAAAA RRTHEHVSEKTSESPSKPGE KKGSDEKKAASLGSSQSSRT YAGGTASATKVSASSGATSK SSSM in isoform 6 and isoform 7. Curated1
Alternative sequenceiVSP_0007429 – 30Missing in isoform 4 and isoform 8. 3 PublicationsAdd BLAST22
Alternative sequenceiVSP_00074344 – 62Missing in isoform 4 and isoform 7. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_000744212 – 224Missing in isoform 2, isoform 3 and isoform 5. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16217 mRNA Translation: BAA03747.1
D50827 mRNA Translation: BAA09438.1
AF327443 mRNA Translation: AAG48151.1
BT009783 mRNA Translation: AAP88785.1
AK296919 mRNA Translation: BAH12454.1
AC008906 Genomic DNA No translation available.
AC020900 Genomic DNA No translation available.
AC104125 Genomic DNA No translation available.
KC877037 Genomic DNA No translation available.
CH471084 Genomic DNA Translation: EAW96068.1
CH471084 Genomic DNA Translation: EAW96071.1
CH471084 Genomic DNA Translation: EAW96075.1
BC013579 mRNA Translation: AAH13579.1
BC016066 mRNA Translation: AAH16066.1 Sequence problems.
AL832349 mRNA No translation available.
M86258 mRNA Translation: AAB59398.1
M28230
, M28227, M28228, M28229 Genomic DNA Translation: AAA52066.1
M33328 mRNA Translation: AAA52296.1
U26724 mRNA Translation: AAC50136.2
S73329 mRNA Translation: AAB32311.1
U38525 mRNA Translation: AAA80684.1
U31345 mRNA Translation: AAB60371.1
U31346 mRNA Translation: AAB60372.1
AF095891 mRNA Translation: AAD09102.1
CCDSiCCDS4082.1 [P20810-8]
CCDS54882.1 [P20810-9]
CCDS54883.1 [P20810-2]
PIRiA38091
RefSeqiNP_001035905.1, NM_001042440.4 [P20810-9]
NP_001035906.1, NM_001042441.2 [P20810-7]
NP_001035907.1, NM_001042442.2 [P20810-10]
NP_001035908.1, NM_001042443.2 [P20810-1]
NP_001035909.1, NM_001042444.2 [P20810-4]
NP_001271141.1, NM_001284212.3
NP_001317560.1, NM_001330631.1 [P20810-2]
NP_001741.4, NM_001750.6 [P20810-6]
NP_775083.1, NM_173060.4 [P20810-8]
XP_006714759.1, XM_006714696.3 [P20810-6]
XP_006714764.1, XM_006714701.3 [P20810-10]
XP_006714766.1, XM_006714703.3 [P20810-5]
XP_006714767.1, XM_006714704.3
XP_016865402.1, XM_017009913.1 [P20810-7]
UniGeneiHs.436186

Genome annotation databases

EnsembliENST00000309190; ENSP00000312523; ENSG00000153113 [P20810-8]
ENST00000338252; ENSP00000343421; ENSG00000153113 [P20810-2]
ENST00000341926; ENSP00000339914; ENSG00000153113 [P20810-1]
ENST00000395812; ENSP00000379157; ENSG00000153113 [P20810-9]
ENST00000395813; ENSP00000379158; ENSG00000153113 [P20810-1]
ENST00000508830; ENSP00000425721; ENSG00000153113 [P20810-6]
ENST00000510756; ENSP00000422176; ENSG00000153113 [P20810-10]
GeneIDi831
KEGGihsa:831
UCSCiuc003klt.4 human [P20810-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiICAL_HUMAN
AccessioniPrimary (citable) accession number: P20810
Secondary accession number(s): B7Z468
, G5E946, G5E9D3, O95360, Q05DE8, Q7Z4K0, Q96D08, Q9H1Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 20, 2007
Last modified: July 18, 2018
This is version 174 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health