CAPN3

Functionⁱ

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityⁱ

Broad endopeptidase activity. EC:3.4.22.54

Activity regulationⁱ

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	129	PROSITE-ProRule annotation	1
Active siteⁱ	334	PROSITE-ProRule annotation	1
Active siteⁱ	358	PROSITE-ProRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Calcium bindingⁱ	662 – 672	1Combined sources1 PublicationAdd BLAST	11
Calcium bindingⁱ	705 – 716	2PROSITE-ProRule annotationCombined sources1 PublicationAdd BLAST	12
Calcium bindingⁱ	735 – 741	3PROSITE-ProRule annotationCombined sources1 Publication	7
Calcium bindingⁱ	800 – 806	4Combined sources1 Publication	7

GO - Molecular functionⁱ

calcium-dependent cysteine-type endopeptidase activity Source: UniProtKB
calcium ion binding Source: UniProtKB
catalytic activity
Source: UniProtKB
Inferred from direct assayⁱ
- "Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A."
  Ono Y., Shimada H., Sorimachi H., Richard I., Saido T.C., Beckmann J.S., Ishiura S., Suzuki K.
  J Biol Chem 273:17073-17078(1998) [PubMed] [Europe PMC] [Abstract]
cysteine-type peptidase activity
Source: ProtInc
Traceable author statementⁱ
- Ref.8
  "Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and mu-types. Specific expression of the mRNA in skeletal muscle."
  Sorimachi H., Imajoh-Ohmi S., Emori Y., Kawasaki H., Ohno S., Minami Y., Suzuki K.
  J. Biol. Chem. 264:20106-20111(1989) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 44-821 (ISOFORM I).
identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- "A reference map of the human binary protein interactome."
  Luck K., Kim D.K., Lambourne L., Spirohn K., Begg B.E., Bian W., Brignall R., Cafarelli T., Campos-Laborie F.J., Charloteaux B., Choi D., Cote A.G., Daley M., Deimling S., Desbuleux A., Dricot A., Gebbia M., Hardy M.F.
  Calderwood M.A.
  Nature 580:402-408(2020) [PubMed] [Europe PMC] [Abstract]
ligase regulator activity
Source: UniProtKB
Inferred from direct assayⁱ
- "Calpain 3 is a rapid-action, unidirectional proteolytic switch central to muscle remodeling."
  de Morree A., Lutje Hulsik D., Impagliazzo A., van Haagen H.H., de Galan P., van Remoortere A., 't Hoen P.A., van Ommen G.B., Frants R.R., van der Maarel S.M.
  PLoS One 5:e11940-e11940(2010) [PubMed] [Europe PMC] [Abstract]
molecular adaptor activity Source: UniProtKB
peptidase activity
Source: UniProtKB
Inferred from direct assayⁱ
- "Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A."
  Ono Y., Shimada H., Sorimachi H., Richard I., Saido T.C., Beckmann J.S., Ishiura S., Suzuki K.
  J Biol Chem 273:17073-17078(1998) [PubMed] [Europe PMC] [Abstract]
sodium ion binding Source: UniProtKB
structural constituent of muscle Source: UniProtKB
titin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- "Multiple molecular interactions implicate the connectin/titin N2A region as a modulating scaffold for p94/calpain 3 activity in skeletal muscle."
  Hayashi C., Ono Y., Doi N., Kitamura F., Tagami M., Mineki R., Arai T., Taguchi H., Yanagida M., Hirner S., Labeit D., Labeit S., Sorimachi H.
  J Biol Chem 283:14801-14814(2008) [PubMed] [Europe PMC] [Abstract]
- "Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A."
  Ono Y., Shimada H., Sorimachi H., Richard I., Saido T.C., Beckmann J.S., Ishiura S., Suzuki K.
  J Biol Chem 273:17073-17078(1998) [PubMed] [Europe PMC] [Abstract]

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Thiol protease
Ligand	Calcium, Metal-binding

Enzyme and pathway databases

BRENDAⁱ	3.4.22.54, 2681
PathwayCommonsⁱ	P20807
Reactomeⁱ	R-HSA-1474228, Degradation of the extracellular matrix
SIGNORⁱ	P20807

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	C02.004

MEROPSⁱ

C02.004

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calpain-3 (EC:3.4.22.54) Alternative name(s): Calcium-activated neutral proteinase 3 Short name: CANP 3 Calpain L3 Calpain p94 Muscle-specific calcium-activated neutral protease 3 New calpain 1 Short name: nCL-1
Gene namesⁱ	Name:CAPN3 Synonyms:CANP3, CANPL3, NCL1
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 15

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000092529.22
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:1480, CAPN3
MIMⁱ	114240, gene
neXtProtⁱ	NX_P20807

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Muscular dystrophy, limb-girdle, autosomal recessive 1 (LGMDR1)10 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionAn autosomal recessive degenerative myopathy characterized by progressive symmetrical atrophy and weakness of the proximal limb muscles and elevated serum creatine kinase. The symptoms usually begin during the first two decades of life, and the disease gradually worsens, often resulting in loss of walking ability 10 or 20 years after onset.

Related information in OMIM

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_009548	4	V → I in LGMDR1. Corresponds to variant dbSNP:rs140660066EnsemblClinVar.	1
Natural variantⁱVAR_009549	26	P → L in LGMDR1. Corresponds to variant dbSNP:rs762020512EnsemblClinVar.	1
Natural variantⁱVAR_009550	77	D → N in LGMDR1.	1
Natural variantⁱVAR_009551	86	S → F in LGMDR1; severe. 1 PublicationCorresponds to variant dbSNP:rs121434546EnsemblClinVar.	1
Natural variantⁱVAR_009552	93 – 100	Missing in LGMDR1.	8
Natural variantⁱVAR_009554	118	R → G in LGMDR1. Corresponds to variant dbSNP:rs1566973583Ensembl.	1
Natural variantⁱVAR_009555	137	C → R in LGMDR1.	1
Natural variantⁱVAR_009556	162	I → L in LGMDR1.	1
Natural variantⁱVAR_001363	182	L → Q in LGMDR1.	1
Natural variantⁱVAR_009557	183	P → L in LGMDR1.	1
Natural variantⁱVAR_009559	189	L → P in LGMDR1. Corresponds to variant dbSNP:rs758795961EnsemblClinVar.	1
Natural variantⁱVAR_001364	200 – 204	Missing in LGMDR1. 1 Publication	5
Natural variantⁱVAR_009560	214	G → S in LGMDR1. Corresponds to variant dbSNP:rs369784333EnsemblClinVar.	1
Natural variantⁱVAR_009562	215 – 221	Missing in LGMDR1 and LGMDD4. 3 Publications	7
Natural variantⁱVAR_009561	215	S → P in LGMDR1. 1 Publication	1
Natural variantⁱVAR_009563	217	E → K in LGMDR1. Corresponds to variant dbSNP:rs773001194EnsemblClinVar.	1
Natural variantⁱVAR_009564	222	G → R in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs1345121557EnsemblClinVar.	1
Natural variantⁱVAR_009565	226	E → K in LGMDR1.	1
Natural variantⁱVAR_009566	232	T → I in LGMDR1.	1
Natural variantⁱVAR_001365	234	G → E in LGMDR1. Corresponds to variant dbSNP:rs1555420634EnsemblClinVar.	1
Natural variantⁱVAR_009568	254	Missing in LGMDR1.	1
Natural variantⁱVAR_076561	266 – 267	Missing in LGMDR1. 2 Publications	2
Natural variantⁱVAR_009569	319	P → L in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs121434547EnsemblClinVar.	1
Natural variantⁱVAR_009570	334	H → Q in LGMDR1. 1 Publication	1
Natural variantⁱVAR_009571	336	Y → N in LGMDR1. 1 Publication	1
Natural variantⁱVAR_001366	354	V → G in LGMDR1. Corresponds to variant dbSNP:rs1555421271EnsemblClinVar.	1
Natural variantⁱVAR_009572	360	W → C in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs267606703EnsemblClinVar.	1
Natural variantⁱVAR_009573	437	R → C in LGMDR1. Corresponds to variant dbSNP:rs777483913EnsemblClinVar.	1
Natural variantⁱVAR_009574	440	R → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs777323132EnsemblClinVar.	1
Natural variantⁱVAR_009575	441	G → D in LGMDR1.	1
Natural variantⁱVAR_009576	445	G → R in LGMDR1. Corresponds to variant dbSNP:rs773827877EnsemblClinVar.	1
Natural variantⁱVAR_009577	448	R → C in LGMDR1. Corresponds to variant dbSNP:rs776043976EnsemblClinVar.	1
Natural variantⁱVAR_009578	448	R → G in LGMDR1. Corresponds to variant dbSNP:rs776043976EnsemblClinVar.	1
Natural variantⁱVAR_009579	448	R → H in LGMDR1. Corresponds to variant dbSNP:rs863224956EnsemblClinVar.	1
Natural variantⁱVAR_009580	479	S → G in LGMDR1. Corresponds to variant dbSNP:rs201736037EnsemblClinVar.	1
Natural variantⁱVAR_009581	486	Q → E in LGMDR1. 1 Publication	1
Natural variantⁱVAR_009582	489	R → Q in LGMDR1. Corresponds to variant dbSNP:rs147764579EnsemblClinVar.	1
Natural variantⁱVAR_009583	489	R → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs863224957EnsemblClinVar.	1
Natural variantⁱVAR_009584	490	R → Q in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs121434548EnsemblClinVar.	1
Natural variantⁱVAR_001367	490	R → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs141656719EnsemblClinVar.	1
Natural variantⁱVAR_009585	493	R → W in LGMDR1. Corresponds to variant dbSNP:rs557164942EnsemblClinVar.	1
Natural variantⁱVAR_009586	496	G → R in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs761637940Ensembl.	1
Natural variantⁱVAR_009587	502	I → T in LGMDR1. Corresponds to variant dbSNP:rs148044781EnsemblClinVar.	1
Natural variantⁱVAR_009588	541	R → Q in LGMDR1. Corresponds to variant dbSNP:rs398123143EnsemblClinVar.	1
Natural variantⁱVAR_009589	567	G → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs727503839EnsemblClinVar.	1
Natural variantⁱVAR_001368	572	R → Q in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs121434544EnsemblClinVar.	1
Natural variantⁱVAR_009590	572	R → W in LGMDR1. 2 PublicationsCorresponds to variant dbSNP:rs863224959EnsemblClinVar.	1
Natural variantⁱVAR_009591	606	S → L in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs199806879EnsemblClinVar.	1
Natural variantⁱVAR_009592	638	Q → P in LGMDR1.	1
Natural variantⁱVAR_009593	698	R → P in LGMDR1.	1
Natural variantⁱVAR_009594	702	A → V in LGMDR1. 3 PublicationsCorresponds to variant dbSNP:rs886042557EnsemblClinVar.	1
Natural variantⁱVAR_009595	705	D → G in LGMDR1.	1
Natural variantⁱVAR_009596	705	D → H in LGMDR1.	1
Natural variantⁱVAR_009597	731	F → S in LGMDR1.	1
Natural variantⁱVAR_001369	744	S → G in LGMDR1. 2 PublicationsCorresponds to variant dbSNP:rs750083132EnsemblClinVar.	1
Natural variantⁱVAR_009598	748	R → Q in LGMDR1. 5 PublicationsCorresponds to variant dbSNP:rs587780290EnsemblClinVar.	1
Natural variantⁱVAR_001370	769	R → Q in LGMDR1. Corresponds to variant dbSNP:rs80338802EnsemblClinVar.	1
Natural variantⁱVAR_009599	774	H → D in LGMDR1; unknown pathological significance.	1
Natural variantⁱVAR_009600	798	A → E in LGMDR1; unknown pathological significance. Corresponds to variant dbSNP:rs149095128EnsemblClinVar.	1

Muscular dystrophy, limb-girdle, autosomal dominant 4 (LGMDD4)2 Publications

The disease is caused by mutations affecting the gene represented in this entry.

Disease descriptionA form of autosomal dominant limb-girdle muscular dystrophy, a myopathy characterized by proximal and/or distal muscle weakness and atrophy. The age at onset is variable and can range from the first to the sixth decade, although later onset is less common. LGMDD4 is characterized by onset of proximal muscle weakness in young adulthood, gait difficulties, increased serum creatine kinase, myalgia, and back pain. Some patients may have upper limb involvement. Disease severity and expressivity are highly variable.

Related information in OMIM

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_009562	215 – 221	Missing in LGMDR1 and LGMDD4. 3 Publications		7

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	129	C → S: Loss of activity. No effect on CMYA5-binding. 1 Publication		1

Keywords - Diseaseⁱ

Disease mutation, Limb-girdle muscular dystrophy

Organism-specific databases

DisGeNET More...DisGeNETⁱ	825
GeneReviewsⁱ	CAPN3
MalaCards human disease database More...MalaCardsⁱ	CAPN3
MIMⁱ	253600, phenotype 618129, phenotype
Open Targets More...OpenTargetsⁱ	ENSG00000092529
Orphanetⁱ	267, Calpain-3-related limb-girdle muscular dystrophy R1
PharmGKBⁱ	PA26061

Miscellaneous databases

Pharosⁱ	P20807, Tbio

Pharosⁱ

P20807, Tbio

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB06124, L-aminocarnityl-succinyl-leucyl-argininal-diethylacetal

DrugBankⁱ

DB06124, L-aminocarnityl-succinyl-leucyl-argininal-diethylacetal

Polymorphism and mutation databases

BioMutaⁱ	CAPN3

BioMutaⁱ

CAPN3

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000207706	1 – 821	Calpain-3Add BLAST		821

Proteomic databases

jPOSTⁱ	P20807
MassIVEⁱ	P20807
PaxDbⁱ	P20807
PeptideAtlas More...PeptideAtlasⁱ	P20807
PRIDEⁱ	P20807
ProteomicsDBⁱ	53791 [P20807-1] 53792 [P20807-2] 53793 [P20807-3] 53794 [P20807-4] 78657

PTM databases

GlyGenⁱ	P20807, 1 site, 1 O-linked glycan (1 site)
iPTMnetⁱ	P20807
PhosphoSitePlusⁱ	P20807

Expressionⁱ

Tissue specificityⁱ

Isoform I is skeletal muscle specific.

Gene expression databases

Bgeeⁱ	ENSG00000092529, Expressed in muscle of leg and 114 other tissues
ExpressionAtlasⁱ	P20807, baseline and differential
Genevisibleⁱ	P20807, HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	ENSG00000092529, Tissue enhanced (brain, skeletal muscle)

HPAⁱ

ENSG00000092529, Tissue enhanced (brain, skeletal muscle)

Interactionⁱ

Subunit structureⁱ

Homodimer; via EF-hand domain 4 (PubMed:24846670).

Interacts with TTN/titin (PubMed:14583192).

Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis (PubMed:20634290).

3 Publications

Binary interactionsⁱ

Hide details

P20807

With	#Exp.	IntAct
NTAQ1 [Q96HA8]	3	EBI-5655000,EBI-741158
OSGIN1 [Q9UJX0]	4	EBI-5655000,EBI-9057006
TTN [Q8WZ42]	4	EBI-5655000,EBI-681210

Isoform II [P20807-2]

With	#Exp.	IntAct
TFCP2 [Q12800]	3	EBI-16433991,EBI-717422

CAPN3 - isoform IV [P20807-4]

With	#Exp.	IntAct
BYSL [Q13895]	3	EBI-11532021,EBI-358049
itself	3	EBI-11532021,EBI-11532021
CCDC102B [Q68D86]	3	EBI-11532021,EBI-10171570
EHHADH [Q08426]	3	EBI-11532021,EBI-2339219
EXOC8 [Q8IYI6]	3	EBI-11532021,EBI-742102
GTPBP3 [Q969Y2]	3	EBI-11532021,EBI-740290
KANK2 [Q63ZY3]	3	EBI-11532021,EBI-2556193
MAP1LC3C [Q9BXW4]	3	EBI-11532021,EBI-2603996
NTAQ1 [Q96HA8]	3	EBI-11532021,EBI-741158
RUFY3 [Q7L099]	3	EBI-11532021,EBI-722392
VEZF1 [Q14119]	3	EBI-11532021,EBI-11980193
ZNF410 - isoform 3 [Q86VK4-3]	3	EBI-11532021,EBI-11741890

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGRIDⁱ	107275, 18 interactors
IntActⁱ	P20807, 33 interactors
Molecular INTeraction database More...MINTⁱ	P20807
STRINGⁱ	9606.ENSP00000380349

Miscellaneous databases

RNActⁱ	P20807, protein

RNActⁱ

P20807, protein

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	61 – 70	Combined sources	10
Beta strandⁱ	78 – 80	Combined sources	3
Helixⁱ	84 – 87	Combined sources	4
Beta strandⁱ	89 – 91	Combined sources	3
Beta strandⁱ	98 – 100	Combined sources	3
Helixⁱ	102 – 105	Combined sources	4
Beta strandⁱ	106 – 108	Combined sources	3
Beta strandⁱ	113 – 116	Combined sources	4
Helixⁱ	118 – 120	Combined sources	3
Helixⁱ	129 – 138	Combined sources	10
Helixⁱ	142 – 148	Combined sources	7
Beta strandⁱ	155 – 158	Combined sources	4
Beta strandⁱ	161 – 168	Combined sources	8
Beta strandⁱ	170 – 179	Combined sources	10
Beta strandⁱ	182 – 185	Combined sources	4
Beta strandⁱ	188 – 191	Combined sources	4
Helixⁱ	201 – 212	Combined sources	12
Helixⁱ	217 – 219	Combined sources	3
Helixⁱ	224 – 232	Combined sources	9
Beta strandⁱ	235 – 240	Combined sources	6
Helixⁱ	241 – 243	Combined sources	3
Helixⁱ	248 – 257	Combined sources	10
Beta strandⁱ	261 – 265	Combined sources	5
Beta strandⁱ	336 – 346	Combined sources	11
Beta strandⁱ	349 – 357	Combined sources	9
Helixⁱ	374 – 378	Combined sources	5
Helixⁱ	381 – 386	Combined sources	6
Beta strandⁱ	394 – 400	Combined sources	7
Helixⁱ	401 – 407	Combined sources	7
Beta strandⁱ	410 – 415	Combined sources	6
Helixⁱ	654 – 662	Combined sources	9
Turnⁱ	663 – 666	Combined sources	4
Helixⁱ	670 – 680	Combined sources	11
Helixⁱ	694 – 704	Combined sources	11
Beta strandⁱ	708 – 712	Combined sources	5
Helixⁱ	714 – 734	Combined sources	21
Beta strandⁱ	740 – 743	Combined sources	4
Helixⁱ	744 – 753	Combined sources	10
Helixⁱ	760 – 770	Combined sources	11
Beta strandⁱ	775 – 778	Combined sources	4
Helixⁱ	779 – 797	Combined sources	19
Beta strandⁱ	804 – 809	Combined sources	6
Helixⁱ	811 – 821	Combined sources	11

Show more details

3D structure databases

SMRⁱ	P20807
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	74 – 417	Calpain catalyticPROSITE-ProRule annotationAdd BLAST	344
Domainⁱ	649 – 683	EF-hand 1PROSITE-ProRule annotationAdd BLAST	35
Domainⁱ	692 – 725	EF-hand 2PROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	722 – 757	EF-hand 3PROSITE-ProRule annotationAdd BLAST	36
Domainⁱ	787 – 821	EF-hand 4PROSITE-ProRule annotationAdd BLAST	35

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	418 – 586	Domain IIIAdd BLAST	169
Regionⁱ	587 – 649	LinkerAdd BLAST	63
Regionⁱ	650 – 821	Domain IVAdd BLAST	172

Sequence similaritiesⁱ

Belongs to the peptidase C2 family.Curated

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG0045, Eukaryota
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00940000156092
HOGENOMⁱ	CLU_010982_0_1_1
InParanoidⁱ	P20807
OMAⁱ	MEIADNP
Database of Orthologous Groups More...OrthoDBⁱ	704215at2759
PhylomeDBⁱ	P20807
TreeFamⁱ	TF314748

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit
InterProⁱ	View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029531, CAPN3/9 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat
PANTHERⁱ	PTHR10183:SF329, PTHR10183:SF329, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit
PRINTSⁱ	PR00704, CALPAIN
SMARTⁱ	View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit SM00054, EFh, 3 hits
SUPFAMⁱ	SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit
PROSITEⁱ	View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit

Sequences (5+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 5 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 5 described isoforms and 20 potential isoforms that are computationally mapped.Show all Align All

Isoform I (identifier: P20807-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the canonicalⁱ sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTVISASVA PRTAAEPRSP GPVPHPAQSK ATEAGGGNPS GIYSAIISRN 
        60         70         80         90        100
FPIIGVKEKT FEQLHKKCLE KKVLYVDPEF PPDETSLFYS QKFPIQFVWK 
       110        120        130        140        150
RPPEICENPR FIIDGANRTD ICQGELGDCW FLAAIACLTL NQHLLFRVIP 
       160        170        180        190        200
HDQSFIENYA GIFHFQFWRY GEWVDVVIDD CLPTYNNQLV FTKSNHRNEF 
       210        220        230        240        250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVAEFFE IRDAPSDMYK 
       260        270        280        290        300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLLQ 
       310        320        330        340        350
DSDLDPRGSD ERPTRTIIPV QYETRMACGL VRGHAYSVTG LDEVPFKGEK 
       360        370        380        390        400
VKLVRLRNPW GQVEWNGSWS DRWKDWSFVD KDEKARLQHQ VTEDGEFWMS 
       410        420        430        440        450
YEDFIYHFTK LEICNLTADA LQSDKLQTWT VSVNEGRWVR GCSAGGCRNF 
       460        470        480        490        500
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGASLF 
       510        520        530        540        550
TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSKTYINM REVSQRFRLP 
       560        570        580        590        600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEVENTISV DRPVKKKKTK 
       610        620        630        640        650
PIIFVSDRAN SNKELGVDQE SEEGKGKTSP DKQKQSPQPQ PGSSDQESEE 
       660        670        680        690        700
QQQFRNIFKQ IAGDDMEICA DELKKVLNTV VNKHKDLKTH GFTLESCRSM 
       710        720        730        740        750
IALMDTDGSG KLNLQEFHHL WNKIKAWQKI FKHYDTDQSG TINSYEMRNA 
       760        770        780        790        800
VNDAGFHLNN QLYDIITMRY ADKHMNIDFD SFICCFVRLE GMFRAFHAFD 
       810        820 
KDGDGIIKLN VLEWLQLTMY A

Length:821

Mass (Da):94,254

Last modified:February 1, 1996 - v2

Checksum:ⁱBC608E8B67AA2741

GO

Isoform II (identifier: P20807-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
268-315: Missing.
595-638: Missing.

« Hide

        10         20         30         40         50
MPTVISASVA PRTAAEPRSP GPVPHPAQSK ATEAGGGNPS GIYSAIISRN 
        60         70         80         90        100
FPIIGVKEKT FEQLHKKCLE KKVLYVDPEF PPDETSLFYS QKFPIQFVWK 
       110        120        130        140        150
RPPEICENPR FIIDGANRTD ICQGELGDCW FLAAIACLTL NQHLLFRVIP 
       160        170        180        190        200
HDQSFIENYA GIFHFQFWRY GEWVDVVIDD CLPTYNNQLV FTKSNHRNEF 
       210        220        230        240        250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVAEFFE IRDAPSDMYK 
       260        270        280        290        300
IMKKAIERGS LMGCSIDTII PVQYETRMAC GLVRGHAYSV TGLDEVPFKG 
       310        320        330        340        350
EKVKLVRLRN PWGQVEWNGS WSDRWKDWSF VDKDEKARLQ HQVTEDGEFW 
       360        370        380        390        400
MSYEDFIYHF TKLEICNLTA DALQSDKLQT WTVSVNEGRW VRGCSAGGCR 
       410        420        430        440        450
NFPDTFWTNP QYRLKLLEED DDPDDSEVIC SFLVALMQKN RRKDRKLGAS 
       460        470        480        490        500
LFTIGFAIYE VPKEMHGNKQ HLQKDFFLYN ASKARSKTYI NMREVSQRFR 
       510        520        530        540        550
LPPSEYVIVP STYEPHQEGE FILRVFSEKR NLSEEVENTI SVDRPVPQPG 
       560        570        580        590        600
SSDQESEEQQ QFRNIFKQIA GDDMEICADE LKKVLNTVVN KHKDLKTHGF 
       610        620        630        640        650
TLESCRSMIA LMDTDGSGKL NLQEFHHLWN KIKAWQKIFK HYDTDQSGTI 
       660        670        680        690        700
NSYEMRNAVN DAGFHLNNQL YDIITMRYAD KHMNIDFDSF ICCFVRLEGM 
       710        720 
FRAFHAFDKD GDGIIKLNVL EWLQLTMYA

Show »

Length:729

Mass (Da):84,089

Checksum:ⁱDE8265BA362725FD

GO

Isoform III (identifier: P20807-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
595-600: Missing.

« Hide

        10         20         30         40         50
MPTVISASVA PRTAAEPRSP GPVPHPAQSK ATEAGGGNPS GIYSAIISRN 
        60         70         80         90        100
FPIIGVKEKT FEQLHKKCLE KKVLYVDPEF PPDETSLFYS QKFPIQFVWK 
       110        120        130        140        150
RPPEICENPR FIIDGANRTD ICQGELGDCW FLAAIACLTL NQHLLFRVIP 
       160        170        180        190        200
HDQSFIENYA GIFHFQFWRY GEWVDVVIDD CLPTYNNQLV FTKSNHRNEF 
       210        220        230        240        250
WSALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVAEFFE IRDAPSDMYK 
       260        270        280        290        300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LNMGELIARM VRNMDNSLLQ 
       310        320        330        340        350
DSDLDPRGSD ERPTRTIIPV QYETRMACGL VRGHAYSVTG LDEVPFKGEK 
       360        370        380        390        400
VKLVRLRNPW GQVEWNGSWS DRWKDWSFVD KDEKARLQHQ VTEDGEFWMS 
       410        420        430        440        450
YEDFIYHFTK LEICNLTADA LQSDKLQTWT VSVNEGRWVR GCSAGGCRNF 
       460        470        480        490        500
PDTFWTNPQY RLKLLEEDDD PDDSEVICSF LVALMQKNRR KDRKLGASLF 
       510        520        530        540        550
TIGFAIYEVP KEMHGNKQHL QKDFFLYNAS KARSKTYINM REVSQRFRLP 
       560        570        580        590        600
PSEYVIVPST YEPHQEGEFI LRVFSEKRNL SEEVENTISV DRPVPIIFVS 
       610        620        630        640        650
DRANSNKELG VDQESEEGKG KTSPDKQKQS PQPQPGSSDQ ESEEQQQFRN 
       660        670        680        690        700
IFKQIAGDDM EICADELKKV LNTVVNKHKD LKTHGFTLES CRSMIALMDT 
       710        720        730        740        750
DGSGKLNLQE FHHLWNKIKA WQKIFKHYDT DQSGTINSYE MRNAVNDAGF 
       760        770        780        790        800
HLNNQLYDII TMRYADKHMN IDFDSFICCF VRLEGMFRAF HAFDKDGDGI 
       810 
IKLNVLEWLQ LTMYA

Show »

Length:815

Mass (Da):93,512

Checksum:ⁱE51E96C05024617C

GO

Isoform IV (identifier: P20807-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-512: Missing.

« Hide

        10         20         30         40         50
MHGNKQHLQK DFFLYNASKA RSKTYINMRE VSQRFRLPPS EYVIVPSTYE 
        60         70         80         90        100
PHQEGEFILR VFSEKRNLSE EVENTISVDR PVKKKKTKPI IFVSDRANSN 
       110        120        130        140        150
KELGVDQESE EGKGKTSPDK QKQSPQPQPG SSDQESEEQQ QFRNIFKQIA 
       160        170        180        190        200
GDDMEICADE LKKVLNTVVN KHKDLKTHGF TLESCRSMIA LMDTDGSGKL 
       210        220        230        240        250
NLQEFHHLWN KIKAWQKIFK HYDTDQSGTI NSYEMRNAVN DAGFHLNNQL 
       260        270        280        290        300
YDIITMRYAD KHMNIDFDSF ICCFVRLEGM FRAFHAFDKD GDGIIKLNVL 

EWLQLTMYA

Show »

Length:309

Mass (Da):35,930

Checksum:ⁱ8F6F0380D4DE8262

GO

Isoform V (identifier: P20807-5) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-665: Missing.

« Hide

        10         20         30         40         50
MEICADELKK VLNTVVNKHK DLKTHGFTLE SCRSMIALMD TDGSGKLNLQ 
        60         70         80         90        100
EFHHLWNKIK AWQKIFKHYD TDQSGTINSY EMRNAVNDAG FHLNNQLYDI 
       110        120        130        140        150
ITMRYADKHM NIDFDSFICC FVRLEGMFRA FHAFDKDGDG IIKLNVLEWL 

QLTMYA

Show »

Length:156

Mass (Da):18,246

Checksum:ⁱ240D1D4F331C4AA0

GO

Computationally mapped potential isoform sequencesⁱ

There are 20 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name	Protein names	Gene names	Length	Annotation

	F8W8F5	F8W8F5_HUMAN	Calpain-3 Calpain-3	CAPN3	773	Annotation score:
	H3BMH1	H3BMH1_HUMAN	Calpain-3 Calpain-3	CAPN3	255	Annotation score:
	A0A669KAB9	A0A669KAB9_HUMAN	Calpain-3 Calpain-3	CAPN3	202	Annotation score:
	A0A669KB08	A0A669KB08_HUMAN	Calpain-3 Calpain-3	CAPN3	229	Annotation score:
	A0A0S2Z3E6	A0A0S2Z3E6_HUMAN	Calpain 3 isoform 2 Calpain 3 isoform 2 (Calpain-3)	CAPN3	83	Annotation score:
	H3BUZ3	H3BUZ3_HUMAN	Calpain-3 Calpain-3	CAPN3	157	Annotation score:
	H3BV08	H3BV08_HUMAN	Calpain-3 Calpain-3	CAPN3	169	Annotation score:
	H3BS77	H3BS77_HUMAN	Calpain-3 Calpain-3	CAPN3	191	Annotation score:
	H3BSA2	H3BSA2_HUMAN	Calpain-3 Calpain-3	CAPN3	188	Annotation score:
	H3BNN7	H3BNN7_HUMAN	Calpain-3 Calpain-3	CAPN3	110	Annotation score:
There are more potential isoformsShow all

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_009548	4	V → I in LGMDR1. Corresponds to variant dbSNP:rs140660066EnsemblClinVar.	1
Natural variantⁱVAR_022272	21	G → E1 PublicationCorresponds to variant dbSNP:rs28364364EnsemblClinVar.	1
Natural variantⁱVAR_009549	26	P → L in LGMDR1. Corresponds to variant dbSNP:rs762020512EnsemblClinVar.	1
Natural variantⁱVAR_009550	77	D → N in LGMDR1.	1
Natural variantⁱVAR_009551	86	S → F in LGMDR1; severe. 1 PublicationCorresponds to variant dbSNP:rs121434546EnsemblClinVar.	1
Natural variantⁱVAR_009552	93 – 100	Missing in LGMDR1.	8
Natural variantⁱVAR_009553	107	E → K. Corresponds to variant dbSNP:rs1801505EnsemblClinVar.	1
Natural variantⁱVAR_009554	118	R → G in LGMDR1. Corresponds to variant dbSNP:rs1566973583Ensembl.	1
Natural variantⁱVAR_009555	137	C → R in LGMDR1.	1
Natural variantⁱVAR_015389	160	A → G1 PublicationCorresponds to variant dbSNP:rs17592EnsemblClinVar.	1
Natural variantⁱVAR_009556	162	I → L in LGMDR1.	1
Natural variantⁱVAR_001363	182	L → Q in LGMDR1.	1
Natural variantⁱVAR_009557	183	P → L in LGMDR1.	1
Natural variantⁱVAR_009558	184	T → M. Corresponds to variant dbSNP:rs35889956EnsemblClinVar.	1
Natural variantⁱVAR_009559	189	L → P in LGMDR1. Corresponds to variant dbSNP:rs758795961EnsemblClinVar.	1
Natural variantⁱVAR_001364	200 – 204	Missing in LGMDR1. 1 Publication	5
Natural variantⁱVAR_009560	214	G → S in LGMDR1. Corresponds to variant dbSNP:rs369784333EnsemblClinVar.	1
Natural variantⁱVAR_009562	215 – 221	Missing in LGMDR1 and LGMDD4. 3 Publications	7
Natural variantⁱVAR_009561	215	S → P in LGMDR1. 1 Publication	1
Natural variantⁱVAR_009563	217	E → K in LGMDR1. Corresponds to variant dbSNP:rs773001194EnsemblClinVar.	1
Natural variantⁱVAR_009564	222	G → R in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs1345121557EnsemblClinVar.	1
Natural variantⁱVAR_009565	226	E → K in LGMDR1.	1
Natural variantⁱVAR_009566	232	T → I in LGMDR1.	1
Natural variantⁱVAR_001365	234	G → E in LGMDR1. Corresponds to variant dbSNP:rs1555420634EnsemblClinVar.	1
Natural variantⁱVAR_009567	236	A → T1 PublicationCorresponds to variant dbSNP:rs1801449EnsemblClinVar.	1
Natural variantⁱVAR_009568	254	Missing in LGMDR1.	1
Natural variantⁱVAR_076561	266 – 267	Missing in LGMDR1. 2 Publications	2
Natural variantⁱVAR_009569	319	P → L in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs121434547EnsemblClinVar.	1
Natural variantⁱVAR_009570	334	H → Q in LGMDR1. 1 Publication	1
Natural variantⁱVAR_009571	336	Y → N in LGMDR1. 1 Publication	1
Natural variantⁱVAR_001366	354	V → G in LGMDR1. Corresponds to variant dbSNP:rs1555421271EnsemblClinVar.	1
Natural variantⁱVAR_009572	360	W → C in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs267606703EnsemblClinVar.	1
Natural variantⁱVAR_009573	437	R → C in LGMDR1. Corresponds to variant dbSNP:rs777483913EnsemblClinVar.	1
Natural variantⁱVAR_009574	440	R → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs777323132EnsemblClinVar.	1
Natural variantⁱVAR_009575	441	G → D in LGMDR1.	1
Natural variantⁱVAR_009576	445	G → R in LGMDR1. Corresponds to variant dbSNP:rs773827877EnsemblClinVar.	1
Natural variantⁱVAR_009577	448	R → C in LGMDR1. Corresponds to variant dbSNP:rs776043976EnsemblClinVar.	1
Natural variantⁱVAR_009578	448	R → G in LGMDR1. Corresponds to variant dbSNP:rs776043976EnsemblClinVar.	1
Natural variantⁱVAR_009579	448	R → H in LGMDR1. Corresponds to variant dbSNP:rs863224956EnsemblClinVar.	1
Natural variantⁱVAR_009580	479	S → G in LGMDR1. Corresponds to variant dbSNP:rs201736037EnsemblClinVar.	1
Natural variantⁱVAR_009581	486	Q → E in LGMDR1. 1 Publication	1
Natural variantⁱVAR_009582	489	R → Q in LGMDR1. Corresponds to variant dbSNP:rs147764579EnsemblClinVar.	1
Natural variantⁱVAR_009583	489	R → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs863224957EnsemblClinVar.	1
Natural variantⁱVAR_009584	490	R → Q in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs121434548EnsemblClinVar.	1
Natural variantⁱVAR_001367	490	R → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs141656719EnsemblClinVar.	1
Natural variantⁱVAR_009585	493	R → W in LGMDR1. Corresponds to variant dbSNP:rs557164942EnsemblClinVar.	1
Natural variantⁱVAR_009586	496	G → R in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs761637940Ensembl.	1
Natural variantⁱVAR_009587	502	I → T in LGMDR1. Corresponds to variant dbSNP:rs148044781EnsemblClinVar.	1
Natural variantⁱVAR_009588	541	R → Q in LGMDR1. Corresponds to variant dbSNP:rs398123143EnsemblClinVar.	1
Natural variantⁱVAR_009589	567	G → W in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs727503839EnsemblClinVar.	1
Natural variantⁱVAR_001368	572	R → Q in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs121434544EnsemblClinVar.	1
Natural variantⁱVAR_009590	572	R → W in LGMDR1. 2 PublicationsCorresponds to variant dbSNP:rs863224959EnsemblClinVar.	1
Natural variantⁱVAR_009591	606	S → L in LGMDR1. 1 PublicationCorresponds to variant dbSNP:rs199806879EnsemblClinVar.	1
Natural variantⁱVAR_047691	622	E → A. Corresponds to variant dbSNP:rs11557723Ensembl.	1
Natural variantⁱVAR_009592	638	Q → P in LGMDR1.	1
Natural variantⁱVAR_009593	698	R → P in LGMDR1.	1
Natural variantⁱVAR_009594	702	A → V in LGMDR1. 3 PublicationsCorresponds to variant dbSNP:rs886042557EnsemblClinVar.	1
Natural variantⁱVAR_009595	705	D → G in LGMDR1.	1
Natural variantⁱVAR_009596	705	D → H in LGMDR1.	1
Natural variantⁱVAR_009597	731	F → S in LGMDR1.	1
Natural variantⁱVAR_001369	744	S → G in LGMDR1. 2 PublicationsCorresponds to variant dbSNP:rs750083132EnsemblClinVar.	1
Natural variantⁱVAR_009598	748	R → Q in LGMDR1. 5 PublicationsCorresponds to variant dbSNP:rs587780290EnsemblClinVar.	1
Natural variantⁱVAR_001370	769	R → Q in LGMDR1. Corresponds to variant dbSNP:rs80338802EnsemblClinVar.	1
Natural variantⁱVAR_009599	774	H → D in LGMDR1; unknown pathological significance.	1
Natural variantⁱVAR_009600	798	A → E in LGMDR1; unknown pathological significance. Corresponds to variant dbSNP:rs149095128EnsemblClinVar.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_044255	1 – 665	Missing in isoform V. 1 PublicationAdd BLAST	665
Alternative sequenceⁱVSP_007813	1 – 512	Missing in isoform IV. 2 PublicationsAdd BLAST	512
Alternative sequenceⁱVSP_005227	268 – 315	Missing in isoform II. 1 PublicationAdd BLAST	48
Alternative sequenceⁱVSP_005228	595 – 638	Missing in isoform II. 1 PublicationAdd BLAST	44
Alternative sequenceⁱVSP_005229	595 – 600	Missing in isoform III. 1 Publication	6

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X85030 mRNA Translation: CAA59403.1 AF209502 Genomic DNA Translation: AAL40183.1 AF127764 mRNA Translation: AAD28253.1 AF127765 mRNA Translation: AAD28254.3 BT007322 mRNA Translation: AAP35986.1 AY902237 Genomic DNA Translation: AAW69391.1 AC012651 Genomic DNA No translation available. BC003169 mRNA Translation: AAH03169.1 BC003521 mRNA Translation: AAH03521.1 BC004883 mRNA Translation: AAH04883.1 BC007810 mRNA Translation: AAH07810.3 BC067126 mRNA Translation: AAH67126.1 BC100782 mRNA Translation: AAI00783.1 BC107791 mRNA Translation: AAI07792.1 BC128605 mRNA Translation: AAI28606.1 BC146649 mRNA Translation: AAI46650.1 BC146672 mRNA Translation: AAI46673.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS10085.1 [P20807-2] CCDS10086.1 [P20807-5] CCDS32207.1 [P20807-3] CCDS45245.1 [P20807-1] CCDS45246.1 [P20807-4]
PIRⁱ	A56218, CIHUH3
NCBI Reference Sequences More...RefSeqⁱ	NP_000061.1, NM_000070.2 [P20807-1] NP_077320.1, NM_024344.1 [P20807-3] NP_775110.1, NM_173087.1 [P20807-2] NP_775111.1, NM_173088.1 [P20807-4] NP_775112.1, NM_173089.1 [P20807-5] NP_775113.1, NM_173090.1 [P20807-5]

Genome annotation databases

Ensemblⁱ	ENST00000337571; ENSP00000336840; ENSG00000092529 [P20807-5] ENST00000349748; ENSP00000183936; ENSG00000092529 [P20807-2] ENST00000356316; ENSP00000348667; ENSG00000092529 [P20807-5] ENST00000357568; ENSP00000350181; ENSG00000092529 [P20807-3] ENST00000397163; ENSP00000380349; ENSG00000092529 [P20807-1] ENST00000397200; ENSP00000380384; ENSG00000092529 [P20807-4] ENST00000397204; ENSP00000380387; ENSG00000092529 [P20807-5] ENST00000569136; ENSP00000455254; ENSG00000092529 [P20807-5] ENST00000673692; ENSP00000501138; ENSG00000092529 [P20807-5] ENST00000673771; ENSP00000501023; ENSG00000092529 [P20807-5] ENST00000673851; ENSP00000501142; ENSG00000092529 [P20807-5] ENST00000673886; ENSP00000501155; ENSG00000092529 [P20807-5] ENST00000673890; ENSP00000501293; ENSG00000092529 [P20807-5] ENST00000673928; ENSP00000501099; ENSG00000092529 [P20807-5] ENST00000673936; ENSP00000501189; ENSG00000092529 [P20807-5] ENST00000674018; ENSP00000501271; ENSG00000092529 [P20807-5] ENST00000674093; ENSP00000501303; ENSG00000092529 [P20807-5] ENST00000674119; ENSP00000501217; ENSG00000092529 [P20807-5] ENST00000674139; ENSP00000501054; ENSG00000092529 [P20807-5] ENST00000674146; ENSP00000501175; ENSG00000092529 [P20807-5] ENST00000674149; ENSP00000501112; ENSG00000092529 [P20807-5]
GeneIDⁱ	825
KEGGⁱ	hsa:825
UCSC genome browser More...UCSCⁱ	uc001zpn.2, human [P20807-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P20807-4	CAPN3 isoform 6		PANTR		309	UniRef100_P20807-4
Calpain 3 isoform 1 (Fragment)		HUMAN		309
CAPN3 isoform 10 (Fragment)		PONAB		93
Uncharacterized protein		CERAT		156
Uncharacterized protein		NOMLE		156
+12

Protein	Similar proteins		Species	Score	Length	Source
P20807-4	CAPN3 isoform 6		PANTR		309	UniRef90_P20807-4
Calpain 3 isoform 1 (Fragment)		HUMAN		309
CAPN3 isoform 10 (Fragment)		PONAB		93
Uncharacterized protein		CERAT		156
Uncharacterized protein		NOMLE		156
+34

Protein	Similar proteins		Species	Score	Length	Source
P20807	Calpain-3		MACFA		815	UniRef50_P20807
Uncharacterized protein		Paroedura picta		718
Uncharacterized protein		AMPOC		738
Uncharacterized protein		ELEEL		730
Uncharacterized protein		MONAL		744
+373
P20807-2	Calpain-9		MOUSE		690	UniRef50_P20807-2
Calpain-9		RAT		690
calpain-3 isoform X2		DELLE		709
Calpain-9		CRIGR		763
calpain-3 isoform X9		CALUR		729
+359
P20807-4	CAPN3 isoform 6		PANTR		309	UniRef50_P20807-4
Calpain 3 isoform 1 (Fragment)		HUMAN		309
CAPN3 isoform 10 (Fragment)		PONAB		93
Uncharacterized protein		CERAT		156
Uncharacterized protein		NOMLE		156
+48

Cross-referencesⁱ

Web resourcesⁱ

Leiden Muscular Dystrophy pages

Calpain-3 mutations in LGMD2A

NIEHS-SNPs

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X85030 mRNA Translation: CAA59403.1 AF209502 Genomic DNA Translation: AAL40183.1 AF127764 mRNA Translation: AAD28253.1 AF127765 mRNA Translation: AAD28254.3 BT007322 mRNA Translation: AAP35986.1 AY902237 Genomic DNA Translation: AAW69391.1 AC012651 Genomic DNA No translation available. BC003169 mRNA Translation: AAH03169.1 BC003521 mRNA Translation: AAH03521.1 BC004883 mRNA Translation: AAH04883.1 BC007810 mRNA Translation: AAH07810.3 BC067126 mRNA Translation: AAH67126.1 BC100782 mRNA Translation: AAI00783.1 BC107791 mRNA Translation: AAI07792.1 BC128605 mRNA Translation: AAI28606.1 BC146649 mRNA Translation: AAI46650.1 BC146672 mRNA Translation: AAI46673.1
CCDSⁱ	CCDS10085.1 [P20807-2] CCDS10086.1 [P20807-5] CCDS32207.1 [P20807-3] CCDS45245.1 [P20807-1] CCDS45246.1 [P20807-4]
PIRⁱ	A56218, CIHUH3
RefSeqⁱ	NP_000061.1, NM_000070.2 [P20807-1] NP_077320.1, NM_024344.1 [P20807-3] NP_775110.1, NM_173087.1 [P20807-2] NP_775111.1, NM_173088.1 [P20807-4] NP_775112.1, NM_173089.1 [P20807-5] NP_775113.1, NM_173090.1 [P20807-5]

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1Y9V	model	-	A/B	649-821	[»]
	4OKH	X-ray	2.45	A/B/C	642-821	[»]
	6BDT	X-ray	2.30	A/B/C/D	46-419	[»]
	6BGP	X-ray	2.75	A/B/C/D	46-419	[»]
	6BJD	X-ray	2.80	A/B/C/D	46-419	[»]
	6BKJ	X-ray	3.20	A/B/C/D	46-419	[»]
SMRⁱ	P20807
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

BioGRIDⁱ	107275, 18 interactors
IntActⁱ	P20807, 33 interactors
MINTⁱ	P20807
STRINGⁱ	9606.ENSP00000380349

Chemistry databases

DrugBankⁱ	DB06124, L-aminocarnityl-succinyl-leucyl-argininal-diethylacetal

DrugBankⁱ

DB06124, L-aminocarnityl-succinyl-leucyl-argininal-diethylacetal

Protein family/group databases

MEROPSⁱ	C02.004

MEROPSⁱ

C02.004

PTM databases

GlyGenⁱ	P20807, 1 site, 1 O-linked glycan (1 site)
iPTMnetⁱ	P20807
PhosphoSitePlusⁱ	P20807

Polymorphism and mutation databases

BioMutaⁱ	CAPN3

BioMutaⁱ

CAPN3

Proteomic databases

jPOSTⁱ	P20807
MassIVEⁱ	P20807
PaxDbⁱ	P20807
PeptideAtlasⁱ	P20807
PRIDEⁱ	P20807
ProteomicsDBⁱ	53791 [P20807-1] 53792 [P20807-2] 53793 [P20807-3] 53794 [P20807-4] 78657

Protocols and materials databases

Antibodypediaⁱ	10719, 176 antibodies
The DNASU plasmid repository More...DNASUⁱ	825

Genome annotation databases

Ensemblⁱ	ENST00000337571; ENSP00000336840; ENSG00000092529 [P20807-5] ENST00000349748; ENSP00000183936; ENSG00000092529 [P20807-2] ENST00000356316; ENSP00000348667; ENSG00000092529 [P20807-5] ENST00000357568; ENSP00000350181; ENSG00000092529 [P20807-3] ENST00000397163; ENSP00000380349; ENSG00000092529 [P20807-1] ENST00000397200; ENSP00000380384; ENSG00000092529 [P20807-4] ENST00000397204; ENSP00000380387; ENSG00000092529 [P20807-5] ENST00000569136; ENSP00000455254; ENSG00000092529 [P20807-5] ENST00000673692; ENSP00000501138; ENSG00000092529 [P20807-5] ENST00000673771; ENSP00000501023; ENSG00000092529 [P20807-5] ENST00000673851; ENSP00000501142; ENSG00000092529 [P20807-5] ENST00000673886; ENSP00000501155; ENSG00000092529 [P20807-5] ENST00000673890; ENSP00000501293; ENSG00000092529 [P20807-5] ENST00000673928; ENSP00000501099; ENSG00000092529 [P20807-5] ENST00000673936; ENSP00000501189; ENSG00000092529 [P20807-5] ENST00000674018; ENSP00000501271; ENSG00000092529 [P20807-5] ENST00000674093; ENSP00000501303; ENSG00000092529 [P20807-5] ENST00000674119; ENSP00000501217; ENSG00000092529 [P20807-5] ENST00000674139; ENSP00000501054; ENSG00000092529 [P20807-5] ENST00000674146; ENSP00000501175; ENSG00000092529 [P20807-5] ENST00000674149; ENSP00000501112; ENSG00000092529 [P20807-5]
GeneIDⁱ	825
KEGGⁱ	hsa:825
UCSCⁱ	uc001zpn.2, human [P20807-1]

Organism-specific databases

CTDⁱ	825
DisGeNETⁱ	825
EuPathDBⁱ	HostDB:ENSG00000092529.22
GeneCardsⁱ	CAPN3
GeneReviewsⁱ	CAPN3
HGNCⁱ	HGNC:1480, CAPN3
HPAⁱ	ENSG00000092529, Tissue enhanced (brain, skeletal muscle)
MalaCardsⁱ	CAPN3
MIMⁱ	114240, gene 253600, phenotype 618129, phenotype
neXtProtⁱ	NX_P20807
OpenTargetsⁱ	ENSG00000092529
Orphanetⁱ	267, Calpain-3-related limb-girdle muscular dystrophy R1
PharmGKBⁱ	PA26061
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0045, Eukaryota
GeneTreeⁱ	ENSGT00940000156092
HOGENOMⁱ	CLU_010982_0_1_1
InParanoidⁱ	P20807
OMAⁱ	MEIADNP
OrthoDBⁱ	704215at2759
PhylomeDBⁱ	P20807
TreeFamⁱ	TF314748

Enzyme and pathway databases

BRENDAⁱ	3.4.22.54, 2681
PathwayCommonsⁱ	P20807
Reactomeⁱ	R-HSA-1474228, Degradation of the extracellular matrix
SIGNORⁱ	P20807

Miscellaneous databases

BioGRID-ORCSⁱ	825, 5 hits in 846 CRISPR screens
ChiTaRSⁱ	CAPN3, human
GeneWikiⁱ	CAPN3
GenomeRNAiⁱ	825
Pharosⁱ	P20807, Tbio
Protein Ontology More...PROⁱ	PR:P20807
RNActⁱ	P20807, protein
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000092529, Expressed in muscle of leg and 114 other tissues
ExpressionAtlasⁱ	P20807, baseline and differential
Genevisibleⁱ	P20807, HS

Family and domain databases

CDDⁱ	cd00214, Calpain_III, 1 hit cd00044, CysPc, 1 hit
InterProⁱ	View protein in InterPro IPR033883, C2_III IPR022684, Calpain_cysteine_protease IPR022682, Calpain_domain_III IPR022683, Calpain_III IPR036213, Calpain_III_sf IPR029531, CAPN3/9 IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR038765, Papain-like_cys_pep_sf IPR000169, Pept_cys_AS IPR001300, Peptidase_C2_calpain_cat
PANTHERⁱ	PTHR10183:SF329, PTHR10183:SF329, 1 hit
Pfamⁱ	View protein in Pfam PF01067, Calpain_III, 1 hit PF13833, EF-hand_8, 1 hit PF00648, Peptidase_C2, 1 hit
PRINTSⁱ	PR00704, CALPAIN
SMARTⁱ	View protein in SMART SM00720, calpain_III, 1 hit SM00230, CysPc, 1 hit SM00054, EFh, 3 hits
SUPFAMⁱ	SSF47473, SSF47473, 1 hit SSF49758, SSF49758, 1 hit SSF54001, SSF54001, 1 hit
PROSITEⁱ	View protein in PROSITE PS50203, CALPAIN_CAT, 1 hit PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 4 hits PS00139, THIOL_PROTEASE_CYS, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CAN3_HUMAN
Accessionⁱ	P20807Primary (citable) accession number: P20807 Secondary accession number(s): A6H8K6 , Q7L4R0, Q9BQC8, Q9BTU4, Q9Y5S6, Q9Y5S7
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	February 1, 1996
	Last modified:	December 2, 2020
	This is version 229 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Reference proteome

Documents

Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Human chromosome 15
Human chromosome 15: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Peptidase families
Classification of peptidase families and list of entries

Annotation systems

Supporting data

UniProtKB - P20807 (CAN3_HUMAN)

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Calpain-3

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Sites

Regions

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GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Keywords - Diseasei

Organism-specific databases

Miscellaneous databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

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Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

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Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

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<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Catalytic activityⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Web resourcesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ