UniProtKB - P20788 (UCRI_RAT)
Cytochrome b-c1 complex subunit Rieske, mitochondrial
Uqcrfs1
Functioni
Miscellaneous
Caution
Catalytic activityi
- 2 [Fe(III)cytochrome c](out) + a quinol = 2 [Fe(II)cytochrome c](out) + a quinone + 2 H+(out)By similarityEC:7.1.1.8By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 217 | Iron-sulfur (2Fe-2S)By similarity | 1 | |
Metal bindingi | 219 | Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity | 1 | |
Metal bindingi | 236 | Iron-sulfur (2Fe-2S)By similarity | 1 | |
Metal bindingi | 239 | Iron-sulfur (2Fe-2S); via pros nitrogenBy similarity | 1 | |
Metal bindingi | 241 | Iron-sulfur (2Fe-2S)By similarity | 1 |
GO - Molecular functioni
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- oxidoreductase activity Source: GO_Central
- protein-containing complex binding Source: RGD
- ubiquinol-cytochrome-c reductase activity Source: RGD
GO - Biological processi
- mitochondrial electron transport, ubiquinol to cytochrome c Source: GO_Central
- respiratory electron transport chain Source: UniProtKB
- response to antibiotic Source: RGD
- response to drug Source: RGD
- response to hormone Source: RGD
Keywordsi
Molecular function | Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-611105, Respiratory electron transport |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:7.1.1.8)Alternative name(s): Complex III subunit 5 Cytochrome b-c1 complex subunit 5 Liver regeneration-related protein LRRGT00195 Rieske iron-sulfur protein Short name: RISP Rieske protein UQCRFS1Curated Ubiquinol-cytochrome c reductase iron-sulfur subunit Cleaved into the following chain: Cytochrome b-c1 complex subunit 9By similarity Short name: Su9By similarity Short name: Subunit 9By similarity Alternative name(s): 8 kDa subunit 9By similarity Complex III subunit IX Cytochrome b-c1 complex subunit 11 UQCRFS1 mitochondrial targeting sequence Short name: UQCRFS1 MTS Ubiquinol-cytochrome c reductase 8 kDa proteinBy similarity |
Gene namesi | Name:Uqcrfs1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 628838, Uqcrfs1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Single-pass membrane protein By similarity
Mitochondrion
- mitochondrial membrane Source: RGD
- mitochondrial respiratory chain complex III Source: RGD
- mitochondrial respiratory chain complex IV Source: RGD
- mitochondrion Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- respiratory chain complex III Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 79 – 103 | Mitochondrial matrixBy similarityAdd BLAST | 25 | |
Transmembranei | 104 – 140 | HelicalBy similarityAdd BLAST | 37 | |
Topological domaini | 141 – 274 | Mitochondrial intermembraneBy similarityAdd BLAST | 134 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000307248 | 1 – 78 | Cytochrome b-c1 complex subunit 9By similarityAdd BLAST | 78 | |
ChainiPRO_0000030671 | 79 – 274 | Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST | 196 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 222 ↔ 238 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P20788 |
PaxDbi | P20788 |
PRIDEi | P20788 |
PTM databases
iPTMneti | P20788 |
PhosphoSitePlusi | P20788 |
SwissPalmi | P20788 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000018281, Expressed in heart and 20 other tissues |
Genevisiblei | P20788, RN |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI1III2IV1 and megacomplex MCI2III2IV2) (By similarity). Incorporation of the Rieske protein UQCRFS1 is the penultimate step in complex III assembly.
Interacts with TTC19, which is involved in the clearance of UQCRFS1 fragments (By similarity).
By similarityComponent of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII). Subunit 9 corresponds to the mitochondrial targeting sequence (MTS) of Rieske protein UQCRFS1. It is retained after processing and incorporated inside complex III, where it remains bound to the complex and localizes between the 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2.
By similarityProtein-protein interaction databases
BioGRIDi | 253421, 2 interactors |
CORUMi | P20788 |
IntActi | P20788, 1 interactor |
STRINGi | 10116.ENSRNOP00000024609 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 187 – 272 | RieskePROSITE-ProRule annotationAdd BLAST | 86 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1671, Eukaryota |
GeneTreei | ENSGT00390000001014 |
HOGENOMi | CLU_055690_0_1_1 |
InParanoidi | P20788 |
OMAi | EVPTHEF |
OrthoDBi | 1174160at2759 |
PhylomeDBi | P20788 |
TreeFami | TF105037 |
Family and domain databases
Gene3Di | 1.20.5.270, 1 hit 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR037008, bc1_Rieske_TM_sf IPR011070, Globular_prot_asu/bsu IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR014349, Rieske_Fe-S_prot IPR005805, Rieske_Fe-S_prot_C IPR004192, Rieske_TM IPR015248, Ubiqinol_cyt_c_Rdtase_N IPR006317, Ubiquinol_cyt_c_Rdtase_Fe-S-su |
PANTHERi | PTHR10134, PTHR10134, 1 hit |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF09165, Ubiq-Cytc-red_N, 1 hit PF02921, UCR_TM, 1 hit |
PRINTSi | PR00162, RIESKE |
SUPFAMi | SSF50022, SSF50022, 1 hit SSF56568, SSF56568, 1 hit |
TIGRFAMsi | TIGR01416, Rieske_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MLSVAARSGP FAPVLSATSR GVAGALRPLL QSAVPATSEP PVLDVKRPFL
60 70 80 90 100
CRESLSGQAA TRPLVATVGL NVPASVRYSH TDIKVPDFSD YRRAEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NAVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLERVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PTYEFTSGDV VVVG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY539946 mRNA Translation: AAS66286.1 BC085339 mRNA Translation: AAH85339.1 M24542 Genomic DNA Translation: AAA42051.1 |
PIRi | A32296 |
RefSeqi | NP_001008888.1, NM_001008888.1 |
Genome annotation databases
Ensembli | ENSRNOT00000024609; ENSRNOP00000024609; ENSRNOG00000018281 |
GeneIDi | 291103 |
KEGGi | rno:291103 |
UCSCi | RGD:628838, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY539946 mRNA Translation: AAS66286.1 BC085339 mRNA Translation: AAH85339.1 M24542 Genomic DNA Translation: AAA42051.1 |
PIRi | A32296 |
RefSeqi | NP_001008888.1, NM_001008888.1 |
3D structure databases
SMRi | P20788 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 253421, 2 interactors |
CORUMi | P20788 |
IntActi | P20788, 1 interactor |
STRINGi | 10116.ENSRNOP00000024609 |
PTM databases
iPTMneti | P20788 |
PhosphoSitePlusi | P20788 |
SwissPalmi | P20788 |
Proteomic databases
jPOSTi | P20788 |
PaxDbi | P20788 |
PRIDEi | P20788 |
Genome annotation databases
Ensembli | ENSRNOT00000024609; ENSRNOP00000024609; ENSRNOG00000018281 |
GeneIDi | 291103 |
KEGGi | rno:291103 |
UCSCi | RGD:628838, rat |
Organism-specific databases
CTDi | 7386 |
RGDi | 628838, Uqcrfs1 |
Phylogenomic databases
eggNOGi | KOG1671, Eukaryota |
GeneTreei | ENSGT00390000001014 |
HOGENOMi | CLU_055690_0_1_1 |
InParanoidi | P20788 |
OMAi | EVPTHEF |
OrthoDBi | 1174160at2759 |
PhylomeDBi | P20788 |
TreeFami | TF105037 |
Enzyme and pathway databases
Reactomei | R-RNO-611105, Respiratory electron transport |
Miscellaneous databases
PROi | PR:P20788 |
Gene expression databases
Bgeei | ENSRNOG00000018281, Expressed in heart and 20 other tissues |
Genevisiblei | P20788, RN |
Family and domain databases
Gene3Di | 1.20.5.270, 1 hit 2.102.10.10, 1 hit |
InterProi | View protein in InterPro IPR037008, bc1_Rieske_TM_sf IPR011070, Globular_prot_asu/bsu IPR017941, Rieske_2Fe-2S IPR036922, Rieske_2Fe-2S_sf IPR014349, Rieske_Fe-S_prot IPR005805, Rieske_Fe-S_prot_C IPR004192, Rieske_TM IPR015248, Ubiqinol_cyt_c_Rdtase_N IPR006317, Ubiquinol_cyt_c_Rdtase_Fe-S-su |
PANTHERi | PTHR10134, PTHR10134, 1 hit |
Pfami | View protein in Pfam PF00355, Rieske, 1 hit PF09165, Ubiq-Cytc-red_N, 1 hit PF02921, UCR_TM, 1 hit |
PRINTSi | PR00162, RIESKE |
SUPFAMi | SSF50022, SSF50022, 1 hit SSF56568, SSF56568, 1 hit |
TIGRFAMsi | TIGR01416, Rieske_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS51296, RIESKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UCRI_RAT | |
Accessioni | P20788Primary (citable) accession number: P20788 Secondary accession number(s): Q6QI13 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | September 13, 2004 | |
Last modified: | February 10, 2021 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families