UniProtKB - P20782 (ACHE_MOUSE)
Acetylcholine receptor subunit epsilon
Chrne
Functioni
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
GO - Molecular functioni
- acetylcholine-gated cation-selective channel activity Source: MGI
- neurotransmitter receptor activity Source: GO_Central
- transmembrane signaling receptor activity Source: InterPro
- transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential Source: MGI
GO - Biological processi
- acetylcholine receptor signaling pathway Source: ComplexPortal
- cation transport Source: MGI
- chemical synaptic transmission Source: GO_Central
- ion transmembrane transport Source: GO_Central
- nervous system process Source: GO_Central
- regulation of membrane potential Source: MGI
- signal transduction Source: GO_Central
- skeletal muscle contraction Source: MGI
Keywordsi
Molecular function | Ion channel, Ligand-gated ion channel, Receptor |
Biological process | Ion transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors |
Names & Taxonomyi
Protein namesi | Recommended name: Acetylcholine receptor subunit epsilon |
Gene namesi | Name:Chrne Synonyms:Acre |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87894, Chrne |
VEuPathDBi | HostDB:ENSMUSG00000014609 |
Subcellular locationi
Plasma Membrane
- acetylcholine-gated channel complex Source: ComplexPortal
- integral component of plasma membrane Source: GO_Central
- integral component of postsynaptic specialization membrane Source: SynGO
- postsynaptic membrane Source: MGI
Other locations
- neuromuscular junction Source: SynGO
- neuron projection Source: GO_Central
- synapse Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 21 – 239 | ExtracellularAdd BLAST | 219 | |
Transmembranei | 240 – 264 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 265 – 272 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 273 – 291 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 292 – 306 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 307 – 328 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 329 – 456 | CytoplasmicSequence analysisAdd BLAST | 128 | |
Transmembranei | 457 – 480 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 481 – 493 | ExtracellularSequence analysisAdd BLAST | 13 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | Add BLAST | 20 | |
ChainiPRO_0000000330 | 21 – 493 | Acetylcholine receptor subunit epsilonAdd BLAST | 473 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 86 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 148 ↔ 162 | By similarity | ||
Glycosylationi | 161 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P20782 |
PRIDEi | P20782 |
ProteomicsDBi | 285976 |
PTM databases
GlyGeni | P20782, 2 sites |
PhosphoSitePlusi | P20782 |
SwissPalmi | P20782 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000014609, Expressed in spermatid and 88 other tissues |
ExpressionAtlasi | P20782, baseline and differential |
Genevisiblei | P20782, MM |
Interactioni
Subunit structurei
Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains. The muscle heteropentamer composed of alpha-1, beta-1, delta, epsilon subunits interacts with the alpha-conotoxin ImII (By similarity).
By similarityProtein-protein interaction databases
ComplexPortali | CPX-257, Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-epsilon |
STRINGi | 10090.ENSMUSP00000099616 |
Chemistry databases
BindingDBi | P20782 |
Miscellaneous databases
RNActi | P20782, protein |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000160933 |
HOGENOMi | CLU_018074_1_4_1 |
InParanoidi | P20782 |
OMAi | ACNFIAD |
OrthoDBi | 588360at2759 |
TreeFami | TF315605 |
Family and domain databases
Gene3Di | 1.20.58.390, 2 hits 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR038050, Neuro_actylchol_rec IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAGALLGALL LLTLFGRSQG KNEELSLYHH LFDNYDPECR PVRRPEDTVT
60 70 80 90 100
ITLKVTLTNL ISLNEKEETL TTSVWIGIDW HDYRLNYSKD DFAGVGILRV
110 120 130 140 150
PSEHVWLPEI VLENNIDGQF GVAYDSNVLV YEGGYVSWLP PAIYRSTCAV
160 170 180 190 200
EVTYFPFDWQ NCSLIFRSQT YNAEEVEFIF AVDDDGNTIN KIDIDTAAFT
210 220 230 240 250
ENGEWAIDYC PGMIRRYEGG STEGPGETDV IYTLIIRRKP LFYVINIIVP
260 270 280 290 300
CVLISGLVLL AYFLPAQAGG QKCTVSINVL LAQTVFLFLI AQKIPETSLS
310 320 330 340 350
VPLLGRYLIF VMVVATLIVM NCVIVLNVSL RTPTTHATSP RLRQILLELL
360 370 380 390 400
PRLLGSSPPP EDPRTASPAR RASSVGILLR AEELILKKPR SELVFEGQRH
410 420 430 440 450
RHGTWTAALC QNLGAAAPEI RCCVDAVNFV AESTRDQEAT GEELSDWVRM
460 470 480 490
GKALDNVCFW AALVLFSVGS TLIFLGGYFN QVPDLPYPPC IQP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5SXG9 | Q5SXG9_MOUSE | Acetylcholine receptor subunit epsi... | Chrne | 494 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04698 Genomic DNA Translation: AAA37153.1 X55718 mRNA Translation: CAA39251.1 |
CCDSi | CCDS24956.1 |
PIRi | S13592, ACMSE |
RefSeqi | NP_033733.1, NM_009603.1 |
Genome annotation databases
Ensembli | ENSMUST00000102556; ENSMUSP00000099616; ENSMUSG00000014609 |
GeneIDi | 11448 |
KEGGi | mmu:11448 |
UCSCi | uc007jvo.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04698 Genomic DNA Translation: AAA37153.1 X55718 mRNA Translation: CAA39251.1 |
CCDSi | CCDS24956.1 |
PIRi | S13592, ACMSE |
RefSeqi | NP_033733.1, NM_009603.1 |
3D structure databases
AlphaFoldDBi | P20782 |
SMRi | P20782 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-257, Muscle-type nicotinic acetylcholine receptor complex, alpha1-beta1-delta-epsilon |
STRINGi | 10090.ENSMUSP00000099616 |
Chemistry databases
BindingDBi | P20782 |
ChEMBLi | CHEMBL3091265 |
PTM databases
GlyGeni | P20782, 2 sites |
PhosphoSitePlusi | P20782 |
SwissPalmi | P20782 |
Proteomic databases
PaxDbi | P20782 |
PRIDEi | P20782 |
ProteomicsDBi | 285976 |
Protocols and materials databases
Antibodypediai | 11366, 180 antibodies from 27 providers |
DNASUi | 11448 |
Genome annotation databases
Ensembli | ENSMUST00000102556; ENSMUSP00000099616; ENSMUSG00000014609 |
GeneIDi | 11448 |
KEGGi | mmu:11448 |
UCSCi | uc007jvo.1, mouse |
Organism-specific databases
CTDi | 1145 |
MGIi | MGI:87894, Chrne |
VEuPathDBi | HostDB:ENSMUSG00000014609 |
Phylogenomic databases
eggNOGi | KOG3645, Eukaryota |
GeneTreei | ENSGT00940000160933 |
HOGENOMi | CLU_018074_1_4_1 |
InParanoidi | P20782 |
OMAi | ACNFIAD |
OrthoDBi | 588360at2759 |
TreeFami | TF315605 |
Enzyme and pathway databases
Reactomei | R-MMU-629587, Highly sodium permeable postsynaptic acetylcholine nicotinic receptors |
Miscellaneous databases
BioGRID-ORCSi | 11448, 2 hits in 72 CRISPR screens |
PROi | PR:P20782 |
RNActi | P20782, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014609, Expressed in spermatid and 88 other tissues |
ExpressionAtlasi | P20782, baseline and differential |
Genevisiblei | P20782, MM |
Family and domain databases
Gene3Di | 1.20.58.390, 2 hits 2.70.170.10, 1 hit |
InterProi | View protein in InterPro IPR006202, Neur_chan_lig-bd IPR036734, Neur_chan_lig-bd_sf IPR006201, Neur_channel IPR036719, Neuro-gated_channel_TM_sf IPR038050, Neuro_actylchol_rec IPR006029, Neurotrans-gated_channel_TM IPR018000, Neurotransmitter_ion_chnl_CS IPR002394, Nicotinic_acetylcholine_rcpt |
PANTHERi | PTHR18945, PTHR18945, 1 hit |
Pfami | View protein in Pfam PF02931, Neur_chan_LBD, 1 hit PF02932, Neur_chan_memb, 1 hit |
PRINTSi | PR00254, NICOTINICR PR00252, NRIONCHANNEL |
SUPFAMi | SSF63712, SSF63712, 1 hit SSF90112, SSF90112, 1 hit |
PROSITEi | View protein in PROSITE PS00236, NEUROTR_ION_CHANNEL, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACHE_MOUSE | |
Accessioni | P20782Primary (citable) accession number: P20782 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families