UniProtKB - P20735 (GGT1_PIG)
Glutathione hydrolase 1 proenzyme
GGT1
Functioni
Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates and other gamma-glutamyl compounds, such as leukotriene C4 (LTC4). The metabolism of glutathione by GGT1 releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Contributes to cysteine homeostasis, glutathione homeostasis and in the conversion of the leukotriene LTC4 to LTD4.
By similarityCatalytic activityi
- an α-amino acid + an N-terminal (5-L-glutamyl)-[peptide] = 5-L-glutamyl amino acid + N-terminal L-α-aminoacyl-[peptide]By similarityEC:2.3.2.2By similarity
- EC:3.4.19.13By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.4.19.13By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.4.19.14By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
: glutathione metabolism Pathwayi
This protein is involved in the pathway glutathione metabolism, which is part of Sulfur metabolism.View all proteins of this organism that are known to be involved in the pathway glutathione metabolism and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | GlutamateBy similarity | 1 | |
Active sitei | 380 | NucleophileBy similarity | 1 | |
Binding sitei | 419 | GlutamateBy similarity | 1 | |
Binding sitei | 422 | GlutamateBy similarity | 1 | |
Binding sitei | 473 | Glutamate; via amide nitrogenBy similarity | 1 |
GO - Molecular functioni
- glutathione hydrolase activity Source: UniProtKB
- hypoglycin A gamma-glutamyl transpeptidase activity Source: UniProtKB-EC
- leukotriene C4 gamma-glutamyl transferase activity Source: UniProtKB-EC
GO - Biological processi
- glutamate metabolic process Source: UniProtKB
- glutathione biosynthetic process Source: UniProtKB-KW
- glutathione catabolic process Source: UniProtKB
- zymogen activation Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Hydrolase, Protease, Transferase |
Biological process | Glutathione biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00204 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:GGT1 Synonyms:GGT |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 4 | CytoplasmicBy similarity | 4 | |
Transmembranei | 5 – 26 | Helical; Signal-anchor for type II membrane proteinBy similarityAdd BLAST | 22 | |
Topological domaini | 27 – 568 | ExtracellularBy similarityAdd BLAST | 542 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000011062 | 1 – 379 | Glutathione hydrolase 1 heavy chainAdd BLAST | 379 | |
ChainiPRO_0000011063 | 380 – 568 | Glutathione hydrolase 1 light chainAdd BLAST | 189 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 49 ↔ 73 | By similarity | ||
Glycosylationi | 94 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 119 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 191 ↔ 195 | By similarity | ||
Glycosylationi | 229 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 296 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 336 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 343 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 427 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 510 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P20735 |
PeptideAtlasi | P20735 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Heterodimer composed of the light and heavy chains. The active site is located in the light chain.
By similarityProtein-protein interaction databases
STRINGi | 9823.ENSSSCP00000028318 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 398 – 400 | Glutamate bindingBy similarity | 3 | |
Regioni | 450 – 451 | Glutamate bindingBy similarity | 2 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2410, Eukaryota |
InParanoidi | P20735 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
PROSITEi | View protein in PROSITE PS00462, G_GLU_TRANSPEPTIDASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MKKRYLLLAL AAVALVLLIL GLCLWLPSNS KPHNHVYPRA AVAADALRCS
60 70 80 90 100
EIGRDTLRDG GSAVDAAIAA LLCVGLMNAH SMGIGGGLFL TIYNSTTRKA
110 120 130 140 150
EIINAREVAP RLASASMFNS SEQSEEGGLS VAVPGEIRGY ELAHQRHGRL
160 170 180 190 200
PWARLFQPSI ELASQGFPVG KGLAAALERS QDAIKRHPAL CEVFCRNGNV
210 220 230 240 250
LREGDLVTMP RLAKTYETLA VEGAQAFYNG SLTAQIVKDI QEAGGIVTAE
260 270 280 290 300
DLNNYRAELI EQPLRISLGD AQLYAPNAPL SGPVLALILN ILKGYNFSRA
310 320 330 340 350
SVETPEQKGL TYHRIVEAFR FAYAKRTLLG DPKFVNVTEV VRNMSSEFFA
360 370 380 390 400
DQLRARISDT TTHPDSYYEP EFYTPDDAGT AHLSVVSDDG SAVSATSTIN
410 420 430 440 450
LYFGSKVRSR ISGILFNDEM DDFSSPNITN QFGVRPSPAN FITPGKQPLS
460 470 480 490 500
SMCPVIIVGE DGQVRMVVGA SGGTQITTST ALAIIHSLWF GYDVKRAVEE
510 520 530 540 550
PRLHNQLLPN TTTLEKGIDQ AVAAALKTRH HYIQDASTFI GVVQAIVRTP
560
SGWAAASDSR KGGEPAGY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46922 mRNA Translation: CAA87031.1 X16533 Genomic DNA Translation: CAA34536.1 AB271954 mRNA Translation: BAF62329.1 |
PIRi | S05532 |
RefSeqi | NP_999195.1, NM_214030.1 |
Genome annotation databases
GeneIDi | 397095 |
KEGGi | ssc:397095 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z46922 mRNA Translation: CAA87031.1 X16533 Genomic DNA Translation: CAA34536.1 AB271954 mRNA Translation: BAF62329.1 |
PIRi | S05532 |
RefSeqi | NP_999195.1, NM_214030.1 |
3D structure databases
SMRi | P20735 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000028318 |
Proteomic databases
PaxDbi | P20735 |
PeptideAtlasi | P20735 |
Genome annotation databases
GeneIDi | 397095 |
KEGGi | ssc:397095 |
Organism-specific databases
CTDi | 2678 |
Phylogenomic databases
eggNOGi | KOG2410, Eukaryota |
InParanoidi | P20735 |
Enzyme and pathway databases
UniPathwayi | UPA00204 |
Family and domain databases
Gene3Di | 1.10.246.130, 1 hit 3.60.20.40, 1 hit |
InterProi | View protein in InterPro IPR043138, GGT_lsub_C IPR000101, GGT_peptidase IPR043137, GGT_ssub IPR029055, Ntn_hydrolases_N |
PANTHERi | PTHR11686, PTHR11686, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
TIGRFAMsi | TIGR00066, g_glut_trans, 1 hit |
PROSITEi | View protein in PROSITE PS00462, G_GLU_TRANSPEPTIDASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GGT1_PIG | |
Accessioni | P20735Primary (citable) accession number: P20735 Secondary accession number(s): A5A784 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families