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Protein

DNA primase small subunit

Gene

Prim1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.

Miscellaneous

The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei44Sequence analysis1
Active sitei109Sequence analysis1
Active sitei111Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processDNA replication, Transcription
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-113501 Inhibition of replication initiation of damaged DNA by RB1/E2F1
R-MMU-174411 Polymerase switching on the C-strand of the telomere
R-MMU-174430 Telomere C-strand synthesis initiation
R-MMU-68952 DNA replication initiation
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69091 Polymerase switching
R-MMU-69166 Removal of the Flap Intermediate
R-MMU-69183 Processive synthesis on the lagging strand

Names & Taxonomyi

Protein namesi
Recommended name:
DNA primase small subunit (EC:2.7.7.-)
Alternative name(s):
DNA primase 49 kDa subunit
Short name:
p49
Gene namesi
Name:Prim1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:97757 Prim1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Primosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000467311 – 417DNA primase small subunitAdd BLAST417

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP20664
PaxDbiP20664
PeptideAtlasiP20664
PRIDEiP20664

PTM databases

iPTMnetiP20664
PhosphoSitePlusiP20664

Expressioni

Gene expression databases

BgeeiENSMUSG00000025395
CleanExiMM_PRIM1
ExpressionAtlasiP20664 baseline and differential
GenevisibleiP20664 MM

Interactioni

Subunit structurei

Heterodimer of a small subunit and a large subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
Pola1P336094EBI-848742,EBI-688051

Protein-protein interaction databases

BioGridi202361, 2 interactors
ComplexPortaliCPX-2088 DNA polymerase alpha:primase complex
CORUMiP20664
IntActiP20664, 4 interactors
STRINGi10090.ENSMUSP00000026461

Structurei

3D structure databases

ProteinModelPortaliP20664
SMRiP20664
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi121 – 131Zinc knuckle motifAdd BLAST11

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2851 Eukaryota
COG1467 LUCA
GeneTreeiENSGT00390000011466
HOGENOMiHOG000196041
HOVERGENiHBG003006
InParanoidiP20664
KOiK02684
PhylomeDBiP20664
TreeFamiTF312823

Family and domain databases

CDDicd04860 AE_Prim_S, 1 hit
InterProiView protein in InterPro
IPR002755 DNA_primase_S
IPR014052 DNA_primase_ssu_euk/arc
PANTHERiPTHR10536 PTHR10536, 1 hit
PfamiView protein in Pfam
PF01896 DNA_primase_S, 1 hit
TIGRFAMsiTIGR00335 primase_sml, 1 hit

Sequencei

Sequence statusi: Complete.

P20664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPFDPAELP ELLKLYYRRL FPYAQYYRWL NYGGVTKNYF QHREFSFTLK
60 70 80 90 100
DDIYIRYQSF NNQSELEKEM QKMNPYKIDI GAVYSHRPNQ HNTVKLGAFQ
110 120 130 140 150
AQEKELVFDI DMTDYDDVRR CCSSADICSK CWTLMTMAMR IIDRALKEDF
160 170 180 190 200
GFKHRLWVYS GRRGVHCWVC DESVRKLSSA VRSGIVEYLS LVKGGQDVKK
210 220 230 240 250
KVHLNEKVHP FVRKSINIIK KYFEEYALVG QDILENKENW DKILALVPET
260 270 280 290 300
IHDELQRGFQ KFHSSPQRWE HLRKVANSSQ NMKNDKCGPW LEWEVMLQYC
310 320 330 340 350
FPRLDVNVSK GVNHLLKSPF SVHPKTGRIS VPIDFHKVDQ FDPFTVPTIS
360 370 380 390 400
AICRELDMVS THEKEKEENE ADSKHRVRGY KKTSLAPYVK VFEQFLENLD
410
KSRKGELLKK SDLQKDF
Length:417
Mass (Da):49,295
Last modified:February 1, 1991 - v1
Checksum:i54EBF4DA4DE47D8A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti57Y → I in AAA39880 (PubMed:2925677).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04620 mRNA Translation: AAA39880.1
D13544 mRNA Translation: BAA02744.1
CCDSiCCDS36086.1
PIRiA33269
RefSeqiNP_032947.1, NM_008921.2
UniGeneiMm.2903

Genome annotation databases

EnsembliENSMUST00000026461; ENSMUSP00000026461; ENSMUSG00000025395
GeneIDi19075
KEGGimmu:19075
UCSCiuc011xpx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPRI1_MOUSE
AccessioniPrimary (citable) accession number: P20664
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 20, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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