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Protein

Ribosome-inactivating protein saporin-6

Gene

SAP6

Organism
Saponaria officinalis (Common soapwort) (Lychnis saponaria)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2001 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein synthesis inhibitor, Toxin
Biological processPlant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-inactivating protein saporin-6 (EC:3.2.2.22)
Short name:
SAP-6
Short name:
SO-6
Alternative name(s):
rRNA N-glycosidase
Gene namesi
Name:SAP6
OrganismiSaponaria officinalis (Common soapwort) (Lychnis saponaria)
Taxonomic identifieri3572 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeCaryophylleaeSaponaria

Pathology & Biotechi

Protein family/group databases

Allergomei2805 Sap o RIP

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 243 PublicationsAdd BLAST24
ChainiPRO_000003077625 – 277Ribosome-inactivating protein saporin-6Add BLAST253
PropeptideiPRO_0000030777278 – 299Sequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi283N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Seeds and leaves of the plant.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20656
SMRiP20656
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20656

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10, 1 hit
4.10.470.10, 1 hit
InterProiView protein in InterPro
IPR036041 Ribosome-inact_prot_sf
IPR017989 Ribosome_inactivat_1/2
IPR001574 Ribosome_inactivat_prot
IPR017988 Ribosome_inactivat_prot_CS
IPR016138 Ribosome_inactivat_prot_sub1
IPR016139 Ribosome_inactivat_prot_sub2
PfamiView protein in Pfam
PF00161 RIP, 1 hit
PRINTSiPR00396 SHIGARICIN
SUPFAMiSSF56371 SSF56371, 1 hit
PROSITEiView protein in PROSITE
PS00275 SHIGA_RICIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20656-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN
60 70 80 90 100
VKDPNLKYGG TDIAVIGPPS KEKFLRINFQ SSRGTVSLGL KRDNLYVVAY
110 120 130 140 150
LAMDNTNVNR AYYFRSEITS AESTALFPEA TTANQKALEY TEDYQSIEKN
160 170 180 190 200
AQITQGDQSR KELGLGIDLL STSMEAVNKK ARVVKDEARF LLIAIQMTAE
210 220 230 240 250
AARFRYIQNL VIKNFPNKFN SENKVIQFEV NWKKISTAIY GDAKNGVFNK
260 270 280 290
DYDFGFGKVR QVKDLQMGLL MYLGKPKSSN EANSTVRHYG PLKPTLLIT
Length:299
Mass (Da):33,607
Last modified:October 1, 1996 - v2
Checksum:i4BC312958BB4E79B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60G → S AA sequence (PubMed:3925952).Curated1
Sequence conflicti123S → L (PubMed:8454624).Curated1
Sequence conflicti123S → L (PubMed:2325629).Curated1
Sequence conflicti212I → T AA sequence (PubMed:2547612).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72E → D. 1
Natural varianti115R → K. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15655 mRNA Translation: CAA33685.1
S57638 mRNA Translation: AAB25863.1
X69135 Genomic DNA Translation: CAA48889.1
X64917 mRNA Translation: CAA46110.1
PIRiS05205
S16487
S18307
S29931
S38527

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15655 mRNA Translation: CAA33685.1
S57638 mRNA Translation: AAB25863.1
X69135 Genomic DNA Translation: CAA48889.1
X64917 mRNA Translation: CAA46110.1
PIRiS05205
S16487
S18307
S29931
S38527

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QI7X-ray2.00A25-277[»]
ProteinModelPortaliP20656
SMRiP20656
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei2805 Sap o RIP

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP20656

Family and domain databases

Gene3Di3.40.420.10, 1 hit
4.10.470.10, 1 hit
InterProiView protein in InterPro
IPR036041 Ribosome-inact_prot_sf
IPR017989 Ribosome_inactivat_1/2
IPR001574 Ribosome_inactivat_prot
IPR017988 Ribosome_inactivat_prot_CS
IPR016138 Ribosome_inactivat_prot_sub1
IPR016139 Ribosome_inactivat_prot_sub2
PfamiView protein in Pfam
PF00161 RIP, 1 hit
PRINTSiPR00396 SHIGARICIN
SUPFAMiSSF56371 SSF56371, 1 hit
PROSITEiView protein in PROSITE
PS00275 SHIGA_RICIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIP6_SAPOF
AccessioniPrimary (citable) accession number: P20656
Secondary accession number(s): Q41392
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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