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Protein

Protein phosphatase 1A

Gene

Ppm1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling (By similarity). Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mg2+, Mn2+Note: Binds 2 magnesium or manganese ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60Manganese 1By similarity1
Metal bindingi60Manganese 2By similarity1
Metal bindingi61Manganese 1; via carbonyl oxygenBy similarity1
Metal bindingi239Manganese 2By similarity1
Metal bindingi282Manganese 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandMagnesium, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1A (EC:3.1.3.16)
Alternative name(s):
Protein phosphatase 2C isoform alpha
Short name:
PP2C-alpha
Protein phosphatase IA
Gene namesi
Name:Ppm1a
Synonyms:Pp2c1, Pppm1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3373 Ppm1a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000577442 – 382Protein phosphatase 1AAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei375PhosphoserineBy similarity1
Modified residuei377PhosphoserineBy similarity1

Post-translational modificationi

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiP20650
PRIDEiP20650

PTM databases

iPTMnetiP20650
PhosphoSitePlusiP20650

Expressioni

Gene expression databases

GenevisibleiP20650 RN

Interactioni

Subunit structurei

Monomer (By similarity). Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling. Interacts with SMAD2; the interaction dephosphorylates SMAD2 in its C-terminal SXS motif resulting in disruption of the SMAD2/SMAD4 complex, SMAD2 nuclear export and termination of the TGF-beta-mediated signaling (By similarity). Interacts with the phosphorylated form of IKBKB/IKKB (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CACNA1BQ009753EBI-7491743,EBI-1055161From Homo sapiens.

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • R-SMAD binding Source: UniProtKB

Protein-protein interaction databases

BioGridi246798, 1 interactor
IntActiP20650, 4 interactors
MINTiP20650
STRINGi10116.ENSRNOP00000008238

Structurei

3D structure databases

ProteinModelPortaliP20650
SMRiP20650
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 291PPM-type phosphatasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Belongs to the PP2C family.Curated

Phylogenomic databases

eggNOGiKOG0697 Eukaryota
COG0631 LUCA
HOGENOMiHOG000233895
HOVERGENiHBG053647
InParanoidiP20650
KOiK04457
OrthoDBiEOG091G0AJQ
PhylomeDBiP20650
TreeFamiTF313590

Family and domain databases

CDDicd00143 PP2Cc, 1 hit
Gene3Di1.10.10.430, 1 hit
3.60.40.10, 1 hit
InterProiView protein in InterPro
IPR015655 PP2C
IPR000222 PP2C_BS
IPR012911 PP2C_C
IPR036580 PP2C_C_sf
IPR036457 PPM-type_dom_sf
IPR001932 PPM-type_phosphatase_dom
PANTHERiPTHR13832 PTHR13832, 1 hit
PfamiView protein in Pfam
PF00481 PP2C, 1 hit
PF07830 PP2C_C, 1 hit
SMARTiView protein in SMART
SM00332 PP2Cc, 1 hit
SUPFAMiSSF81601 SSF81601, 1 hit
SSF81606 SSF81606, 1 hit
PROSITEiView protein in PROSITE
PS01032 PPM_1, 1 hit
PS51746 PPM_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20650-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAFLDKPKM EKHNAQGQGN GLRYGLSSMQ GWRVEMEDAH TAVIGLPSGL
60 70 80 90 100
ETWSFFAVYD GHAGSQVAKY CCEHLLDHIT NNQDFKGSAG APSVENVKNG
110 120 130 140 150
IRTGFLEIDE HMRVMSEKKH GADRSGSTAV GVLISPQHTY FINCGDSRGL
160 170 180 190 200
LCRNRKVHFF TQDHKPSNPL EKERIQNAGG SVMIQRVNGS LAVSRALGDF
210 220 230 240 250
DYKCVHGKGP TEQLVSPEPE VHDIERSEED DQFIILACDG IWDVMGNEEL
260 270 280 290 300
CDFVRSRLEV TDDLEKVCNE VVDTCLYKGS RDNMSVILIC FPNAPKVSAE
310 320 330 340 350
AVKKEAELDK YLENRVEEII KKQGEGVPDL VHVMRTLASE NIPSLPPGGE
360 370 380
LASKRNVIEA VYNRLNPYKN DDTDSASTDD MW
Length:382
Mass (Da):42,417
Last modified:February 1, 1991 - v1
Checksum:iC1C386E935374F89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04503 mRNA Translation: AAA41917.1
PIRiA32399
RefSeqiNP_058734.1, NM_017038.1
XP_017449523.1, XM_017594034.1
UniGeneiRn.37403

Genome annotation databases

GeneIDi24666
KEGGirno:24666
UCSCiRGD:3373 rat

Similar proteinsi

Entry informationi

Entry nameiPPM1A_RAT
AccessioniPrimary (citable) accession number: P20650
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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