AHA1

Functionⁱ

The plasma membrane H⁺ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H⁺-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. Forms a functional cation-translocating unit with CNGC17 that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes (PubMed:26071421).1 Publication

Catalytic activityⁱ

ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out).Curated

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	329	4-aspartylphosphate intermediateBy similarity	1
Metal bindingⁱ	588	MagnesiumBy similarity	1
Metal bindingⁱ	592	MagnesiumBy similarity	1

GO - Molecular functionⁱ

ATPase activity
Source: TAIR
Inferred from mutant phenotypeⁱ
- 17557075
ATP binding Source: UniProtKB-KW
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 8300635
proton-exporting ATPase activity, phosphorylative mechanism
Source: UniProtKB
Inferred from direct assayⁱ
- 8300635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ATP hydrolysis coupled proton transport
Source: UniProtKB
Inferred from direct assayⁱ
- 8300635
proton export across plasma membrane Source: InterPro
regulation of stomatal movement
Source: TAIR
Inferred from mutant phenotypeⁱ
- 12047634
response to abscisic acid
Source: TAIR
Inferred from mutant phenotypeⁱ
- 12047634
response to water deprivation
Source: TAIR
Inferred from mutant phenotypeⁱ
- 12047634
stomatal opening
Source: TAIR
Inferred from mutant phenotypeⁱ
- 27261063

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase
Biological process	Hydrogen ion transport, Ion transport, Transport
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	ARA:AT2G18960-MONOMER

BioCycⁱ

ARA:AT2G18960-MONOMER

Protein family/group databases

TCDBⁱ	3.A.3.3.7 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.3.7 the p-type atpase (p-atpase) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATPase 1, plasma membrane-type1 Publication (EC:3.6.3.6Curated) Alternative name(s): Proton pump 1
Gene namesⁱ	Name:AHA11 Publication Ordered Locus Names:At2g18960Imported ORF Names:F19F24.16Imported
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 2

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT2G18960
TAIRⁱ	locus:2044450 AT2G18960

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	2 – 61	CytoplasmicSequence analysisAdd BLAST	60
Transmembraneⁱ	62 – 81	Helical; Name=1Sequence analysisAdd BLAST	20
Topological domainⁱ	82 – 93	ExtracellularSequence analysisAdd BLAST	12
Transmembraneⁱ	94 – 114	Helical; Name=2Sequence analysisAdd BLAST	21
Topological domainⁱ	115 – 243	CytoplasmicSequence analysisAdd BLAST	129
Transmembraneⁱ	244 – 264	Helical; Name=3Sequence analysisAdd BLAST	21
Topological domainⁱ	265 – 273	ExtracellularSequence analysis	9
Transmembraneⁱ	274 – 291	Helical; Name=4Sequence analysisAdd BLAST	18
Topological domainⁱ	292 – 643	CytoplasmicSequence analysisAdd BLAST	352
Transmembraneⁱ	644 – 665	Helical; Name=5Sequence analysisAdd BLAST	22
Topological domainⁱ	666 – 670	ExtracellularSequence analysis	5
Transmembraneⁱ	671 – 693	Helical; Name=6Sequence analysisAdd BLAST	23
Topological domainⁱ	694 – 709	CytoplasmicSequence analysisAdd BLAST	16
Transmembraneⁱ	710 – 730	Helical; Name=7Sequence analysisAdd BLAST	21
Topological domainⁱ	731 – 751	ExtracellularSequence analysisAdd BLAST	21
Transmembraneⁱ	752 – 772	Helical; Name=8Sequence analysisAdd BLAST	21
Topological domainⁱ	773 – 784	CytoplasmicSequence analysisAdd BLAST	12
Transmembraneⁱ	785 – 805	Helical; Name=9Sequence analysisAdd BLAST	21
Topological domainⁱ	806 – 813	ExtracellularSequence analysis	8
Transmembraneⁱ	814 – 834	Helical; Name=10Sequence analysisAdd BLAST	21
Topological domainⁱ	835 – 949	CytoplasmicSequence analysisAdd BLAST	115

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Disruption phenotypeⁱ

No visible phenotype, due to the redudancy with AHA2. Aha1 and aha2 double mutants are embryo lethal.1 Publication

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000046274	2 – 949	ATPase 1, plasma membrane-typeAdd BLAST		948

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineBy similarity	1
Modified residueⁱ	881	PhosphothreonineCombined sources	1
Modified residueⁱ	899	PhosphoserineBy similarity	1
Modified residueⁱ	931	PhosphoserineBy similarity	1
Modified residueⁱ	948	PhosphothreonineCombined sources	1

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

PaxDbⁱ	P20649
PRIDEⁱ	P20649

PTM databases

iPTMnetⁱ	P20649
SwissPalmⁱ	P20649

Expressionⁱ

Developmental stageⁱ

Expressed on the surface of developing seeds and from 8- to 16-cell stages to the heart stage of embryo development.1 Publication

Gene expression databases

ExpressionAtlasⁱ	P20649 baseline and differential
Genevisibleⁱ	P20649 AT

Interactionⁱ

Subunit structureⁱ

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H⁺-ATPase (By similarity). Interacts with PPI1; this interaction promotes ATPase activity. Interacts with PSY1R (PubMed:25267325). Part of a functional complex containing PSKR1, BAK1, CNGC17, and AHA (PubMed:26071421). Interacts with CNGC17 and PSKR1 (PubMed:26071421).By similarity4 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
PPI1	O23144	7	EBI-2354448,EBI-2354477

With

Entry

#Exp.

IntAct

Notes

PPI1

O23144

7

EBI-2354448,EBI-2354477

Protein-protein interaction databases

BioGridⁱ	1770, 12 interactors
IntActⁱ	P20649, 4 interactors
Molecular INTeraction database More...MINTⁱ	P20649
STRINGⁱ	3702.AT2G18960.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P20649
SMRⁱ	P20649
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	947 – 949	Interaction with 14-3-3 proteinsBy similarity		3

Sequence similaritiesⁱ

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0205 Eukaryota COG0474 LUCA
HOGENOMⁱ	HOG000160005
InParanoidⁱ	P20649
KEGG Orthology (KO) More...KOⁱ	K01535
OMAⁱ	HKYNVVE
Database of Orthologous Groups More...OrthoDBⁱ	EOG093602AD
PhylomeDBⁱ	P20649

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02076 P-type_ATPase_H, 1 hit
Gene3Dⁱ	3.40.1110.10, 1 hit
InterProⁱ	View protein in InterPro IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR006534 P-type_ATPase_IIIA IPR001757 P_typ_ATPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00690 Cation_ATPase_N, 1 hit
PRINTSⁱ	PR00120 HATPASE
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81665 SSF81665, 3 hits
TIGRFAMsⁱ	TIGR01647 ATPase-IIIA_H, 1 hit TIGR01494 ATPase_P-type, 2 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P20649-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSGLEDIKNE TVDLEKIPIE EVFQQLKCTR EGLTTQEGED RIVIFGPNKL 
        60         70         80         90        100
EEKKESKILK FLGFMWNPLS WVMEAAALMA IALANGDNRP PDWQDFVGII 
       110        120        130        140        150
CLLVINSTIS FIEENNAGNA AAALMAGLAP KTKVLRDGKW SEQEAAILVP 
       160        170        180        190        200
GDIVSIKLGD IIPADARLLE GDPLKVDQSA LTGESLPVTK HPGQEVFSGS 
       210        220        230        240        250
TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL TSIGNFCICS 
       260        270        280        290        300
IAIGIAIEIV VMYPIQHRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG 
       310        320        330        340        350
SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC 
       360        370        380        390        400
KGVEKDQVLL FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF 
       410        420        430        440        450
NPVDKRTALT YIDSDGNWHR VSKGAPEQIL DLANARPDLR KKVLSCIDKY 
       460        470        480        490        500
AERGLRSLAV ARQVVPEKTK ESPGGPWEFV GLLPLFDPPR HDSAETIRRA 
       510        520        530        540        550
LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSAALLGT DKDSNIASIP 
       560        570        580        590        600
VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD 
       610        620        630        640        650
IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV 
       660        670        680        690        700
SITIRIVFGF MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT 
       710        720        730        740        750
PDSWKLKEIF ATGIVLGGYQ AIMSVIFFWA AHKTDFFSDK FGVRSIRDNN 
       760        770        780        790        800
DELMGAVYLQ VSIISQALIF VTRSRSWSFV ERPGALLMIA FVIAQLVATL 
       810        820        830        840        850
IAVYADWTFA KVKGIGWGWA GVIWIYSIVT YFPQDILKFA IRYILSGKAW 
       860        870        880        890        900
ASLFDNRTAF TTKKDYGIGE REAQWAQAQR TLHGLQPKED VNIFPEKGSY 
       910        920        930        940 
RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVAKLKGLDI DTAGHHYTV

Length:949

Mass (Da):104,224

Last modified:January 23, 2007 - v3

Checksum:ⁱA613FF6070ECC9F3

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1P8AYX4	A0A1P8AYX4_ARATH		Plasma membrane ATPase Plasma membrane ATPase, EC 3.6.3.6	HA1 AHA1, H(+)-ATPase 1, OPEN STOMATA 2, OST2, PLASMA MEMBRANE PROTON ATPASE	885	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	771	V → L in AAA32813 (PubMed:2521951).Curated		1
Sequence conflictⁱ	778	S → Y in AAA32813 (PubMed:2521951).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M24107 mRNA Translation: AAA32813.1 AC003673 Genomic DNA Translation: AAC09030.1 CP002685 Genomic DNA Translation: AEC06832.1 BT008692 mRNA Translation: AAP40498.1
PIRⁱ	T01624 PXMUP1
NCBI Reference Sequences More...RefSeqⁱ	NP_179486.1, NM_127453.4
UniGeneⁱ	At.24695

Genome annotation databases

EnsemblPlantsⁱ	AT2G18960.1; AT2G18960.1; AT2G18960
GeneIDⁱ	816413
Grameneⁱ	AT2G18960.1; AT2G18960.1; AT2G18960
KEGGⁱ	ath:AT2G18960

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P20649	Plasma membrane ATPase		ARATH		949	UniRef100_P20649
Plasma membrane ATPase		ARATH		885
Plasma membrane proton ATPase		ARATH		262

Protein	Similar proteins		Species	Score	Length	Source
P20649	Plasma membrane ATPase		ARATH		949	UniRef90_P20649
Plasma membrane ATPase		NOCCA		949
Plasma membrane ATPase		BRARP		949
Plasma membrane ATPase		Capsella rubella		949
Plasma membrane ATPase		EUTSA		949
+29

Protein	Similar proteins		Species	Score	Length	Source
P20649	ATPase 2, plasma membrane-type	)	ARATH		948	UniRef50_P19456
Plasma membrane ATPase 4		NICPL		952
Plasma membrane ATPase		EUTHA		948
Plasma membrane ATPase		ARAAL		948
ATPase 2, plasma membrane-type (Fragment)		NOCCA		179
+1402

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M24107 mRNA Translation: AAA32813.1 AC003673 Genomic DNA Translation: AAC09030.1 CP002685 Genomic DNA Translation: AEC06832.1 BT008692 mRNA Translation: AAP40498.1
PIRⁱ	T01624 PXMUP1
RefSeqⁱ	NP_179486.1, NM_127453.4
UniGeneⁱ	At.24695

3D structure databases

ProteinModelPortalⁱ	P20649
SMRⁱ	P20649
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	1770, 12 interactors
IntActⁱ	P20649, 4 interactors
MINTⁱ	P20649
STRINGⁱ	3702.AT2G18960.1

Protein family/group databases

TCDBⁱ	3.A.3.3.7 the p-type atpase (p-atpase) superfamily

TCDBⁱ

3.A.3.3.7 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetⁱ	P20649
SwissPalmⁱ	P20649

Proteomic databases

PaxDbⁱ	P20649
PRIDEⁱ	P20649

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT2G18960.1; AT2G18960.1; AT2G18960
GeneIDⁱ	816413
Grameneⁱ	AT2G18960.1; AT2G18960.1; AT2G18960
KEGGⁱ	ath:AT2G18960

Organism-specific databases

Araportⁱ	AT2G18960
TAIRⁱ	locus:2044450 AT2G18960

Phylogenomic databases

eggNOGⁱ	KOG0205 Eukaryota COG0474 LUCA
HOGENOMⁱ	HOG000160005
InParanoidⁱ	P20649
KOⁱ	K01535
OMAⁱ	HKYNVVE
OrthoDBⁱ	EOG093602AD
PhylomeDBⁱ	P20649

Enzyme and pathway databases

BioCycⁱ	ARA:AT2G18960-MONOMER

BioCycⁱ

ARA:AT2G18960-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P20649

PROⁱ

PR:P20649

Gene expression databases

ExpressionAtlasⁱ	P20649 baseline and differential
Genevisibleⁱ	P20649 AT

Family and domain databases

CDDⁱ	cd02076 P-type_ATPase_H, 1 hit
Gene3Dⁱ	3.40.1110.10, 1 hit
InterProⁱ	View protein in InterPro IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR006534 P-type_ATPase_IIIA IPR001757 P_typ_ATPase
Pfamⁱ	View protein in Pfam PF00690 Cation_ATPase_N, 1 hit
PRINTSⁱ	PR00120 HATPASE
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81665 SSF81665, 3 hits
TIGRFAMsⁱ	TIGR01647 ATPase-IIIA_H, 1 hit TIGR01494 ATPase_P-type, 2 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PMA1_ARATH
Accessionⁱ	P20649Primary (citable) accession number: P20649 Secondary accession number(s): O64626
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	January 23, 2007
	Last modified:	April 25, 2018
	This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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Supporting data

UniProtKB - P20649 (PMA1_ARATH)

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ATPase 1, plasma membrane-type

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Golgi apparatus

Nucleus

Plasma Membrane

Vacuole

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ