UniProtKB - P20649 (PMA1_ARATH)
ATPase 1, plasma membrane-type
AHA1
Functioni
Catalytic activityi
- EC:7.1.2.1Curated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 329 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 588 | MagnesiumBy similarity | 1 | |
Metal bindingi | 592 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase activity Source: TAIR
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: UniProtKB
- proton-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB
GO - Biological processi
- proton export across plasma membrane Source: InterPro
- proton transmembrane transport Source: UniProtKB
- regulation of intracellular pH Source: GO_Central
- regulation of stomatal movement Source: TAIR
- response to abscisic acid Source: TAIR
- response to water deprivation Source: TAIR
- stomatal opening Source: TAIR
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G18960-MONOMER |
Protein family/group databases
TCDBi | 3.A.3.3.7, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ATPase 1, plasma membrane-type1 Publication (EC:7.1.2.1Curated)Alternative name(s): Proton pump 1 |
Gene namesi | Name:AHA11 Publication Ordered Locus Names:At2g18960Imported ORF Names:F19F24.16Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G18960 |
TAIRi | locus:2044450, AT2G18960 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication1 Publication; Multi-pass membrane protein 1 Publication
Golgi apparatus
- Golgi apparatus Source: TAIR
Nucleus
- nucleus Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Vacuole
- vacuole Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB
- membrane Source: TAIR
- plasmodesma Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 61 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 62 – 81 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Topological domaini | 82 – 93 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 94 – 114 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 115 – 243 | CytoplasmicSequence analysisAdd BLAST | 129 | |
Transmembranei | 244 – 264 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 265 – 273 | ExtracellularSequence analysis | 9 | |
Transmembranei | 274 – 291 | Helical; Name=4Sequence analysisAdd BLAST | 18 | |
Topological domaini | 292 – 643 | CytoplasmicSequence analysisAdd BLAST | 352 | |
Transmembranei | 644 – 665 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 666 – 670 | ExtracellularSequence analysis | 5 | |
Transmembranei | 671 – 693 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 694 – 709 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 710 – 730 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 731 – 751 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 752 – 772 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 773 – 784 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 785 – 805 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 806 – 813 | ExtracellularSequence analysis | 8 | |
Transmembranei | 814 – 834 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 835 – 949 | CytoplasmicSequence analysisAdd BLAST | 115 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000046274 | 2 – 949 | ATPase 1, plasma membrane-typeAdd BLAST | 948 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 881 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 899 | PhosphoserineBy similarity | 1 | |
Modified residuei | 931 | PhosphoserineBy similarity | 1 | |
Modified residuei | 948 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P20649 |
PRIDEi | P20649 |
ProteomicsDBi | 234928 |
PTM databases
iPTMneti | P20649 |
SwissPalmi | P20649 |
Expressioni
Developmental stagei
Gene expression databases
ExpressionAtlasi | P20649, baseline and differential |
Genevisiblei | P20649, AT |
Interactioni
Subunit structurei
Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).
Interacts with PPI1; this interaction promotes ATPase activity.
Interacts with PSY1R (PubMed:25267325).
Part of a functional complex containing PSKR1, BAK1, CNGC17, and AHA (PubMed:26071421).
Interacts with CNGC17 and PSKR1 (PubMed:26071421).
By similarity4 PublicationsBinary interactionsi
P20649
With | #Exp. | IntAct |
---|---|---|
PPI1 [O23144] | 7 | EBI-2354448,EBI-2354477 |
Protein-protein interaction databases
BioGRIDi | 1770, 12 interactors |
IntActi | P20649, 4 interactors |
MINTi | P20649 |
STRINGi | 3702.AT2G18960.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 947 – 949 | Interaction with 14-3-3 proteinsBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_4_1 |
InParanoidi | P20649 |
OrthoDBi | 188115at2759 |
PhylomeDBi | P20649 |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSGLEDIKNE TVDLEKIPIE EVFQQLKCTR EGLTTQEGED RIVIFGPNKL
60 70 80 90 100
EEKKESKILK FLGFMWNPLS WVMEAAALMA IALANGDNRP PDWQDFVGII
110 120 130 140 150
CLLVINSTIS FIEENNAGNA AAALMAGLAP KTKVLRDGKW SEQEAAILVP
160 170 180 190 200
GDIVSIKLGD IIPADARLLE GDPLKVDQSA LTGESLPVTK HPGQEVFSGS
210 220 230 240 250
TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL TSIGNFCICS
260 270 280 290 300
IAIGIAIEIV VMYPIQHRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG
310 320 330 340 350
SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC
360 370 380 390 400
KGVEKDQVLL FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF
410 420 430 440 450
NPVDKRTALT YIDSDGNWHR VSKGAPEQIL DLANARPDLR KKVLSCIDKY
460 470 480 490 500
AERGLRSLAV ARQVVPEKTK ESPGGPWEFV GLLPLFDPPR HDSAETIRRA
510 520 530 540 550
LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSAALLGT DKDSNIASIP
560 570 580 590 600
VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD
610 620 630 640 650
IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV
660 670 680 690 700
SITIRIVFGF MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT
710 720 730 740 750
PDSWKLKEIF ATGIVLGGYQ AIMSVIFFWA AHKTDFFSDK FGVRSIRDNN
760 770 780 790 800
DELMGAVYLQ VSIISQALIF VTRSRSWSFV ERPGALLMIA FVIAQLVATL
810 820 830 840 850
IAVYADWTFA KVKGIGWGWA GVIWIYSIVT YFPQDILKFA IRYILSGKAW
860 870 880 890 900
ASLFDNRTAF TTKKDYGIGE REAQWAQAQR TLHGLQPKED VNIFPEKGSY
910 920 930 940
RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVAKLKGLDI DTAGHHYTV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AYX4 | A0A1P8AYX4_ARATH | Plasma membrane ATPase | HA1 AHA1, H(+)-ATPase 1, OPEN STOMATA 2, OST2, PLASMA MEMBRANE PROTON ATPASE | 885 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 771 | V → L in AAA32813 (PubMed:2521951).Curated | 1 | |
Sequence conflicti | 778 | S → Y in AAA32813 (PubMed:2521951).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24107 mRNA Translation: AAA32813.1 AC003673 Genomic DNA Translation: AAC09030.1 CP002685 Genomic DNA Translation: AEC06832.1 BT008692 mRNA Translation: AAP40498.1 |
PIRi | T01624, PXMUP1 |
RefSeqi | NP_179486.1, NM_127453.4 |
Genome annotation databases
EnsemblPlantsi | AT2G18960.1; AT2G18960.1; AT2G18960 |
GeneIDi | 816413 |
Gramenei | AT2G18960.1; AT2G18960.1; AT2G18960 |
KEGGi | ath:AT2G18960 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24107 mRNA Translation: AAA32813.1 AC003673 Genomic DNA Translation: AAC09030.1 CP002685 Genomic DNA Translation: AEC06832.1 BT008692 mRNA Translation: AAP40498.1 |
PIRi | T01624, PXMUP1 |
RefSeqi | NP_179486.1, NM_127453.4 |
3D structure databases
SMRi | P20649 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1770, 12 interactors |
IntActi | P20649, 4 interactors |
MINTi | P20649 |
STRINGi | 3702.AT2G18960.1 |
Protein family/group databases
TCDBi | 3.A.3.3.7, the p-type atpase (p-atpase) superfamily |
PTM databases
iPTMneti | P20649 |
SwissPalmi | P20649 |
Proteomic databases
PaxDbi | P20649 |
PRIDEi | P20649 |
ProteomicsDBi | 234928 |
Genome annotation databases
EnsemblPlantsi | AT2G18960.1; AT2G18960.1; AT2G18960 |
GeneIDi | 816413 |
Gramenei | AT2G18960.1; AT2G18960.1; AT2G18960 |
KEGGi | ath:AT2G18960 |
Organism-specific databases
Araporti | AT2G18960 |
TAIRi | locus:2044450, AT2G18960 |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_4_1 |
InParanoidi | P20649 |
OrthoDBi | 188115at2759 |
PhylomeDBi | P20649 |
Enzyme and pathway databases
BioCyci | ARA:AT2G18960-MONOMER |
Miscellaneous databases
PROi | PR:P20649 |
Gene expression databases
ExpressionAtlasi | P20649, baseline and differential |
Genevisiblei | P20649, AT |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA1_ARATH | |
Accessioni | P20649Primary (citable) accession number: P20649 Secondary accession number(s): O64626 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 188 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families