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Protein

ATPase 1, plasma membrane-type

Gene

AHA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses. Forms a functional cation-translocating unit with CNGC17 that is activated by PSKR1/BAK1 and possibly other BAK1/RLK complexes (PubMed:26071421).1 Publication

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3294-aspartylphosphate intermediateBy similarity1
Metal bindingi588MagnesiumBy similarity1
Metal bindingi592MagnesiumBy similarity1

GO - Molecular functioni

  • ATPase activity Source: TAIR
  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: UniProtKB
  • proton-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G18960-MONOMER

Protein family/group databases

TCDBi3.A.3.3.7 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 1, plasma membrane-type1 Publication (EC:3.6.3.6Curated)
Alternative name(s):
Proton pump 1
Gene namesi
Name:AHA11 Publication
Ordered Locus Names:At2g18960Imported
ORF Names:F19F24.16Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G18960
TAIRilocus:2044450 AT2G18960

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 61CytoplasmicSequence analysisAdd BLAST60
Transmembranei62 – 81Helical; Name=1Sequence analysisAdd BLAST20
Topological domaini82 – 93ExtracellularSequence analysisAdd BLAST12
Transmembranei94 – 114Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini115 – 243CytoplasmicSequence analysisAdd BLAST129
Transmembranei244 – 264Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini265 – 273ExtracellularSequence analysis9
Transmembranei274 – 291Helical; Name=4Sequence analysisAdd BLAST18
Topological domaini292 – 643CytoplasmicSequence analysisAdd BLAST352
Transmembranei644 – 665Helical; Name=5Sequence analysisAdd BLAST22
Topological domaini666 – 670ExtracellularSequence analysis5
Transmembranei671 – 693Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini694 – 709CytoplasmicSequence analysisAdd BLAST16
Transmembranei710 – 730Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini731 – 751ExtracellularSequence analysisAdd BLAST21
Transmembranei752 – 772Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini773 – 784CytoplasmicSequence analysisAdd BLAST12
Transmembranei785 – 805Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini806 – 813ExtracellularSequence analysis8
Transmembranei814 – 834Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini835 – 949CytoplasmicSequence analysisAdd BLAST115

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype, due to the redudancy with AHA2. Aha1 and aha2 double mutants are embryo lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000462742 – 949ATPase 1, plasma membrane-typeAdd BLAST948

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei881PhosphothreonineCombined sources1
Modified residuei899PhosphoserineBy similarity1
Modified residuei931PhosphoserineBy similarity1
Modified residuei948PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP20649
PRIDEiP20649

PTM databases

iPTMnetiP20649
SwissPalmiP20649

Expressioni

Developmental stagei

Expressed on the surface of developing seeds and from 8- to 16-cell stages to the heart stage of embryo development.1 Publication

Gene expression databases

ExpressionAtlasiP20649 baseline and differential
GenevisibleiP20649 AT

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity). Interacts with PPI1; this interaction promotes ATPase activity. Interacts with PSY1R (PubMed:25267325). Part of a functional complex containing PSKR1, BAK1, CNGC17, and AHA (PubMed:26071421). Interacts with CNGC17 and PSKR1 (PubMed:26071421).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PPI1O231447EBI-2354448,EBI-2354477

Protein-protein interaction databases

BioGridi1770, 12 interactors
IntActiP20649, 4 interactors
MINTiP20649
STRINGi3702.AT2G18960.1

Structurei

3D structure databases

ProteinModelPortaliP20649
SMRiP20649
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni947 – 949Interaction with 14-3-3 proteinsBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205 Eukaryota
COG0474 LUCA
HOGENOMiHOG000160005
InParanoidiP20649
KOiK01535
OMAiHKYNVVE
OrthoDBiEOG093602AD
PhylomeDBiP20649

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P20649-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGLEDIKNE TVDLEKIPIE EVFQQLKCTR EGLTTQEGED RIVIFGPNKL
60 70 80 90 100
EEKKESKILK FLGFMWNPLS WVMEAAALMA IALANGDNRP PDWQDFVGII
110 120 130 140 150
CLLVINSTIS FIEENNAGNA AAALMAGLAP KTKVLRDGKW SEQEAAILVP
160 170 180 190 200
GDIVSIKLGD IIPADARLLE GDPLKVDQSA LTGESLPVTK HPGQEVFSGS
210 220 230 240 250
TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL TSIGNFCICS
260 270 280 290 300
IAIGIAIEIV VMYPIQHRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG
310 320 330 340 350
SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC
360 370 380 390 400
KGVEKDQVLL FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF
410 420 430 440 450
NPVDKRTALT YIDSDGNWHR VSKGAPEQIL DLANARPDLR KKVLSCIDKY
460 470 480 490 500
AERGLRSLAV ARQVVPEKTK ESPGGPWEFV GLLPLFDPPR HDSAETIRRA
510 520 530 540 550
LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSAALLGT DKDSNIASIP
560 570 580 590 600
VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD
610 620 630 640 650
IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV
660 670 680 690 700
SITIRIVFGF MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT
710 720 730 740 750
PDSWKLKEIF ATGIVLGGYQ AIMSVIFFWA AHKTDFFSDK FGVRSIRDNN
760 770 780 790 800
DELMGAVYLQ VSIISQALIF VTRSRSWSFV ERPGALLMIA FVIAQLVATL
810 820 830 840 850
IAVYADWTFA KVKGIGWGWA GVIWIYSIVT YFPQDILKFA IRYILSGKAW
860 870 880 890 900
ASLFDNRTAF TTKKDYGIGE REAQWAQAQR TLHGLQPKED VNIFPEKGSY
910 920 930 940
RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVAKLKGLDI DTAGHHYTV
Length:949
Mass (Da):104,224
Last modified:January 23, 2007 - v3
Checksum:iA613FF6070ECC9F3
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1P8AYX4A0A1P8AYX4_ARATH
Plasma membrane ATPase
HA1 AHA1, H(+)-ATPase 1, OPEN STOMATA 2, OST2, PLASMA MEMBRANE PROTON ATPASE HA1 AHA1, H(+)-ATPase 1, OPEN STOMATA 2, OST2, PLASMA MEMBRANE PROTON ATPASE, PMA, At2g18960, F19F24.16, F19F24_16
885Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti771V → L in AAA32813 (PubMed:2521951).Curated1
Sequence conflicti778S → Y in AAA32813 (PubMed:2521951).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24107 mRNA Translation: AAA32813.1
AC003673 Genomic DNA Translation: AAC09030.1
CP002685 Genomic DNA Translation: AEC06832.1
BT008692 mRNA Translation: AAP40498.1
PIRiT01624 PXMUP1
RefSeqiNP_179486.1, NM_127453.4
UniGeneiAt.24695

Genome annotation databases

EnsemblPlantsiAT2G18960.1; AT2G18960.1; AT2G18960
GeneIDi816413
GrameneiAT2G18960.1; AT2G18960.1; AT2G18960
KEGGiath:AT2G18960

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24107 mRNA Translation: AAA32813.1
AC003673 Genomic DNA Translation: AAC09030.1
CP002685 Genomic DNA Translation: AEC06832.1
BT008692 mRNA Translation: AAP40498.1
PIRiT01624 PXMUP1
RefSeqiNP_179486.1, NM_127453.4
UniGeneiAt.24695

3D structure databases

ProteinModelPortaliP20649
SMRiP20649
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1770, 12 interactors
IntActiP20649, 4 interactors
MINTiP20649
STRINGi3702.AT2G18960.1

Protein family/group databases

TCDBi3.A.3.3.7 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetiP20649
SwissPalmiP20649

Proteomic databases

PaxDbiP20649
PRIDEiP20649

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G18960.1; AT2G18960.1; AT2G18960
GeneIDi816413
GrameneiAT2G18960.1; AT2G18960.1; AT2G18960
KEGGiath:AT2G18960

Organism-specific databases

AraportiAT2G18960
TAIRilocus:2044450 AT2G18960

Phylogenomic databases

eggNOGiKOG0205 Eukaryota
COG0474 LUCA
HOGENOMiHOG000160005
InParanoidiP20649
KOiK01535
OMAiHKYNVVE
OrthoDBiEOG093602AD
PhylomeDBiP20649

Enzyme and pathway databases

BioCyciARA:AT2G18960-MONOMER

Miscellaneous databases

PROiPR:P20649

Gene expression databases

ExpressionAtlasiP20649 baseline and differential
GenevisibleiP20649 AT

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 2 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPMA1_ARATH
AccessioniPrimary (citable) accession number: P20649
Secondary accession number(s): O64626
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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