UniProtKB - P20646 (PPAP_RAT)
Prostatic acid phosphatase
Acp3
Functioni
A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
1 PublicationIn addition to its tyrosine phosphatase activity, also has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP. This extracellular adenosine leads to a decrease in chronic pain by activating A1R in nociceptive neurons.
By similarityCatalytic activityi
- EC:3.1.3.21 Publication
- EC:3.1.3.5By similarity
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphate + H2O = 1-(9Z-octadecenoyl)-sn-glycerol + phosphateBy similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:3.1.3.48By similarity
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 42 | SubstrateBy similarity | 1 | |
Active sitei | 43 | Nucleophile1 Publication | 1 | |
Binding sitei | 46 | SubstrateBy similarity | 1 | |
Sitei | 48 | Important for substrate specificityBy similarity | 1 | |
Binding sitei | 110 | SubstrateBy similarity | 1 | |
Sitei | 137 | Required for dimerization1 Publication | 1 | |
Sitei | 143 | Required for dimerization1 Publication | 1 | |
Sitei | 205 | Required for structural stabilityBy similarity | 1 | |
Binding sitei | 288 | SubstrateBy similarity | 1 | |
Active sitei | 289 | Proton donor1 Publication | 1 |
GO - Molecular functioni
- 5'-nucleotidase activity Source: RGD
- acid phosphatase activity Source: RGD
- choline binding Source: RGD
- identical protein binding Source: RGD
- lysophosphatidic acid phosphatase activity Source: RGD
- phosphatase activity Source: RGD
- protein homodimerization activity Source: UniProtKB
- protein tyrosine phosphatase activity Source: UniProtKB-EC
- thiamine phosphate phosphatase activity Source: RGD
GO - Biological processi
- adenosine metabolic process Source: RGD
- dephosphorylation Source: RGD
- lipid metabolic process Source: UniProtKB-KW
- lysosome organization Source: GO_Central
- nucleotide metabolic process Source: RGD
- positive regulation of adenosine receptor signaling pathway Source: RGD
- purine nucleobase metabolic process Source: RGD
- regulation of sensory perception of pain Source: RGD
- thiamine metabolic process Source: RGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Acp3 Synonyms:Acpp |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2023, Acpp |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Lysosome
- Lysosome membrane By similarity; Single-pass type I membrane protein Sequence analysis
Note: Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles. Colocalizes with LAMP2 on the lysosome membrane.By similarity
Endosome
- multivesicular body Source: RGD
Extracellular region or secreted
- extracellular space Source: RGD
Golgi apparatus
- Golgi cisterna Source: RGD
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
- lysosome Source: GO_Central
Plasma Membrane
- plasma membrane Source: RGD
Other locations
- apical part of cell Source: RGD
- filopodium Source: RGD
- integral component of membrane Source: RGD
- secretory granule Source: RGD
- vesicle membrane Source: RGD
Keywords - Cellular componenti
Cell membrane, Lysosome, Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 137 | W → E: Abolishes most of enzyme activity and dimer formation. No enzyme activity nor dimer formation; when associated with D-143. 1 Publication | 1 | |
Mutagenesisi | 143 | H → D: Abolishes most of enzyme activity and dimer formation. No enzyme activity nor dimer formation; when associated with E-137. 1 Publication | 1 | |
Mutagenesisi | 154 | Y → K: PH optimum at 5.4 as for wild type and no change in specific activity. Lower pH maximum around 4.5 and more sensitive to L(+)tartrate inhibition but no change in specific activity; when associated with G-158. 1 Publication | 1 | |
Mutagenesisi | 158 | R → G: Broader pH maximum levels around 5.4 but no change in specific activity. Lower pH maximum around 4.5 and more sensitive to L(+)tartrate inhibition but no change in specific activity; when associated with K-154. 1 Publication | 1 | |
Mutagenesisi | 289 | D → A or S: Abolishes almost all enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 289 | D → N: Abolishes enzyme activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 31 | By similarityAdd BLAST | 31 | |
ChainiPRO_0000023964 | 32 – 381 | Prostatic acid phosphataseAdd BLAST | 350 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 93 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 160 ↔ 371 | By similarity | ||
Disulfide bondi | 214 ↔ 312 | By similarity | ||
Glycosylationi | 219 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 332 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 346 ↔ 350 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P20646 |
PTM databases
GlyGeni | P20646, 3 sites |
iPTMneti | P20646 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000011820, Expressed in esophagus and 20 other tissues |
Interactioni
Subunit structurei
Homodimer; dimer formation is required for phosphatase activity.
3 PublicationsGO - Molecular functioni
- identical protein binding Source: RGD
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000016222 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P20646 |
SMRi | P20646 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20646 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3720, Eukaryota |
GeneTreei | ENSGT00940000160450 |
InParanoidi | P20646 |
OMAi | GMKQHYE |
OrthoDBi | 1221585at2759 |
PhylomeDBi | P20646 |
TreeFami | TF312893 |
Family and domain databases
CDDi | cd07061, HP_HAP_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR033379, Acid_Pase_AS IPR000560, His_Pase_clade-2 IPR029033, His_PPase_superfam |
Pfami | View protein in Pfam PF00328, His_Phos_2, 1 hit |
SUPFAMi | SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00616, HIS_ACID_PHOSPHAT_1, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRAVPLHLVG TASLTLGFLL LLSLRLDPGQ AKELKFVTLV FRHGDRGPIE
60 70 80 90 100
TFPNDPIKES SWPQGFGQLT KWGMGQHYEL GSYIRRRYGR FLNNSYKHDQ
110 120 130 140 150
VYIRSTDVDR TLMSAMTNLA ALFPPEGISI WNPRLLWQPI PVHTVSLSED
160 170 180 190 200
RLLYLPFRDC PRFQELKSET LKSEEFLKRL QPYKSFIDTL PSLSGFEDQD
210 220 230 240 250
LFEIWSRLYD PLYCESVHNF TLPTWATEDA MTKLKELSEL SLLSLYGIHK
260 270 280 290 300
QKEKSRLQGG VLVNEILKNM KLATQPQKAR KLIMYSAHDT TVSGLQMALD
310 320 330 340 350
VYNGLLPPYA SCHIMELYQD NGGHFVEMYY RNETQNEPYP LTLPGCTHSC
360 370 380
PLEKFAELLD PVIPQDWATE CMGTSNHQAS L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K4B4 | A0A0G2K4B4_RAT | Acid phosphatase, prostate | Acpp | 417 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 222 – 223 | LP → FR in AAA41806 (PubMed:2373368).Curated | 2 | |
Sequence conflicti | 288 | H → Y in AAA41806 (PubMed:2373368).Curated | 1 | |
Sequence conflicti | 300 – 301 | DV → EL in AAA41806 (PubMed:2373368).Curated | 2 | |
Sequence conflicti | 324 | H → T in AAA41806 (PubMed:2373368).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_036025 | 379 – 381 | ASL → VLRVILATTFCLVTGILVIL LLVLIRHGPCWQRDVYRNI in isoform 2. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32397 mRNA Translation: AAA41806.1 DQ826426 mRNA Translation: ABH07387.1 AABR07071383 Genomic DNA No translation available. |
PIRi | JH0152 |
RefSeqi | NP_001128373.1, NM_001134901.1 [P20646-2] NP_064457.1, NM_020072.1 [P20646-1] |
Genome annotation databases
Ensembli | ENSRNOT00000016222; ENSRNOP00000016222; ENSRNOG00000011820 [P20646-1] ENSRNOT00000085585; ENSRNOP00000072975; ENSRNOG00000011820 [P20646-2] |
GeneIDi | 56780 |
KEGGi | rno:56780 |
UCSCi | RGD:2023, rat [P20646-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32397 mRNA Translation: AAA41806.1 DQ826426 mRNA Translation: ABH07387.1 AABR07071383 Genomic DNA No translation available. |
PIRi | JH0152 |
RefSeqi | NP_001128373.1, NM_001134901.1 [P20646-2] NP_064457.1, NM_020072.1 [P20646-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1RPA | X-ray | 3.00 | A | 32-373 | [»] | |
1RPT | X-ray | 3.00 | A | 32-373 | [»] | |
AlphaFoldDBi | P20646 | |||||
SMRi | P20646 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000016222 |
PTM databases
GlyGeni | P20646, 3 sites |
iPTMneti | P20646 |
Proteomic databases
PaxDbi | P20646 |
Genome annotation databases
Ensembli | ENSRNOT00000016222; ENSRNOP00000016222; ENSRNOG00000011820 [P20646-1] ENSRNOT00000085585; ENSRNOP00000072975; ENSRNOG00000011820 [P20646-2] |
GeneIDi | 56780 |
KEGGi | rno:56780 |
UCSCi | RGD:2023, rat [P20646-1] |
Organism-specific databases
CTDi | 55 |
RGDi | 2023, Acpp |
Phylogenomic databases
eggNOGi | KOG3720, Eukaryota |
GeneTreei | ENSGT00940000160450 |
InParanoidi | P20646 |
OMAi | GMKQHYE |
OrthoDBi | 1221585at2759 |
PhylomeDBi | P20646 |
TreeFami | TF312893 |
Enzyme and pathway databases
Reactomei | R-RNO-6798695, Neutrophil degranulation |
Miscellaneous databases
EvolutionaryTracei | P20646 |
PROi | PR:P20646 |
Gene expression databases
Bgeei | ENSRNOG00000011820, Expressed in esophagus and 20 other tissues |
Family and domain databases
CDDi | cd07061, HP_HAP_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR033379, Acid_Pase_AS IPR000560, His_Pase_clade-2 IPR029033, His_PPase_superfam |
Pfami | View protein in Pfam PF00328, His_Phos_2, 1 hit |
SUPFAMi | SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00616, HIS_ACID_PHOSPHAT_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPAP_RAT | |
Accessioni | P20646Primary (citable) accession number: P20646 Secondary accession number(s): A0A0G2JSL5, A6XJQ5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 10, 2021 | |
Last modified: | May 25, 2022 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families