UniProtKB - P20645 (MPRD_HUMAN)
Protein
Cation-dependent mannose-6-phosphate receptor
Gene
M6PR
Organism
Homo sapiens (Human)
Status
Functioni
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex.
Miscellaneous
This receptor has optimal binding in the presence of divalent cations.
GO - Molecular functioni
- protein domain specific binding Source: InterPro
- retromer complex binding Source: ParkinsonsUK-UCL
- transmembrane signaling receptor activity Source: ProtInc
GO - Biological processi
- endosome to lysosome transport Source: ProtInc
- lysosomal transport Source: UniProtKB
- membrane organization Source: Reactome
- protein targeting to lysosome Source: GO_Central
- receptor-mediated endocytosis Source: ProtInc
- secretion of lysosomal enzymes Source: Ensembl
Keywordsi
Molecular function | Receptor |
Biological process | Transport |
Enzyme and pathway databases
PathwayCommonsi | P20645 |
Reactomei | R-HSA-432720, Lysosome Vesicle Biogenesis R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis |
SIGNORi | P20645 |
Protein family/group databases
TCDBi | 9.B.247.1.1, the mannose 6-phosphate receptor (m6pr) family |
Names & Taxonomyi
Protein namesi | Recommended name: Cation-dependent mannose-6-phosphate receptorShort name: CD Man-6-P receptor Short name: CD-MPR Alternative name(s): 46 kDa mannose 6-phosphate receptor Short name: MPR 46 |
Gene namesi | Name:M6PR Synonyms:MPR46, MPRD |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6752, M6PR |
MIMi | 154540, gene |
neXtProti | NX_P20645 |
VEuPathDBi | HostDB:ENSG00000003056.7 |
Subcellular locationi
Endosome
- endosome Source: MGI
- late endosome Source: Ensembl
Golgi apparatus
- trans-Golgi network Source: GO_Central
- trans-Golgi network membrane Source: Reactome
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: ProtInc
- plasma membrane Source: Reactome
Other locations
- clathrin-coated vesicle membrane Source: Reactome
- membrane Source: UniProtKB
- perinuclear region of cytoplasm Source: UniProtKB
- transport vesicle Source: Reactome
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 27 – 185 | LumenalSequence analysisAdd BLAST | 159 | |
Transmembranei | 186 – 210 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 211 – 277 | CytoplasmicSequence analysisAdd BLAST | 67 |
Keywords - Cellular componenti
Lysosome, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 4074 |
OpenTargetsi | ENSG00000003056 |
PharmGKBi | PA30513 |
Miscellaneous databases
Pharosi | P20645, Tbio |
Chemistry databases
ChEMBLi | CHEMBL5788 |
DrugBanki | DB02755, 1-3 Sugar Ring of Pentamannosyl 6-Phosphate DB01272, Alglucosidase alfa DB02900, alpha-D-mannose 6-phosphate |
Genetic variation databases
BioMutai | M6PR |
DMDMi | 127293 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | Add BLAST | 26 | |
ChainiPRO_0000019226 | 27 – 277 | Cation-dependent mannose-6-phosphate receptorAdd BLAST | 251 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 32 ↔ 78 | PROSITE-ProRule annotation | ||
Glycosylationi | 57 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 83 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 94 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 107 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 113 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 132 ↔ 167 | PROSITE-ProRule annotation | ||
Disulfide bondi | 145 ↔ 179 | PROSITE-ProRule annotation | ||
Modified residuei | 267 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, PhosphoproteinProteomic databases
CPTACi | CPTAC-1308 |
EPDi | P20645 |
jPOSTi | P20645 |
MassIVEi | P20645 |
MaxQBi | P20645 |
PaxDbi | P20645 |
PeptideAtlasi | P20645 |
PRIDEi | P20645 |
ProteomicsDBi | 53769 |
PTM databases
GlyConnecti | 1083, 20 N-Linked glycans (2 sites) |
GlyGeni | P20645, 6 sites, 1 N-linked glycan (1 site) |
iPTMneti | P20645 |
MetOSitei | P20645 |
PhosphoSitePlusi | P20645 |
SwissPalmi | P20645 |
Expressioni
Gene expression databases
Bgeei | ENSG00000003056, Expressed in small intestine Peyer's patch and 245 other tissues |
ExpressionAtlasi | P20645, baseline and differential |
Genevisiblei | P20645, HS |
Organism-specific databases
HPAi | ENSG00000003056, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer. Binds GGA1, GGA2 and GGA3.
Binary interactionsi
Hide detailsP20645
GO - Molecular functioni
- protein domain specific binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 110251, 41 interactors |
ELMi | P20645 |
IntActi | P20645, 16 interactors |
MINTi | P20645 |
STRINGi | 9606.ENSP00000000412 |
Chemistry databases
BindingDBi | P20645 |
Miscellaneous databases
RNActi | P20645, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 181 | MRHPROSITE-ProRule annotationAdd BLAST | 152 |
Domaini
The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the cation-independent mannose 6-phosphate receptor.
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QTJ5, Eukaryota |
GeneTreei | ENSGT00390000002109 |
HOGENOMi | CLU_058440_0_0_1 |
InParanoidi | P20645 |
OMAi | RVCREVN |
OrthoDBi | 1214298at2759 |
PhylomeDBi | P20645 |
TreeFami | TF328910 |
Family and domain databases
Gene3Di | 2.70.130.10, 1 hit |
InterProi | View protein in InterPro IPR028927, Man-6-P_rcpt IPR000296, Man-6-P_rcpt_cation_dep IPR009011, Man6P_isomerase_rcpt-bd_dom_sf |
Pfami | View protein in Pfam PF02157, Man-6-P_recep, 1 hit |
PRINTSi | PR00715, MAN6PRECEPTR |
PROSITEi | View protein in PROSITE PS51914, MRH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 10 potential isoforms that are computationally mapped.Show allAlign All
P20645-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFPFYSCWRT GLLLLLLAVA VRESWQTEEK TCDLVGEKGK ESEKELALVK
60 70 80 90 100
RLKPLFNKSF ESTVGQGSDT YIYIFRVCRE AGNHTSGAGL VQINKSNGKE
110 120 130 140 150
TVVGRLNETH IFNGSNWIML IYKGGDEYDN HCGKEQRRAV VMISCNRHTL
160 170 180 190 200
ADNFNPVSEE RGKVQDCFYL FEMDSSLACS PEISHLSVGS ILLVTFASLV
210 220 230 240 250
AVYVVGGFLY QRLVVGAKGM EQFPHLAFWQ DLGNLVADGC DFVCRSKPRN
260 270
VPAAYRGVGD DQLGEESEER DDHLLPM
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GX30 | F5GX30_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 191 | Annotation score: | ||
H0YF90 | H0YF90_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 148 | Annotation score: | ||
F5GXE0 | F5GXE0_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 92 | Annotation score: | ||
F5GXU0 | F5GXU0_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 109 | Annotation score: | ||
F5H883 | F5H883_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 133 | Annotation score: | ||
H0YGT2 | H0YGT2_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 122 | Annotation score: | ||
H0YGE9 | H0YGE9_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 104 | Annotation score: | ||
H0YFY9 | H0YFY9_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 85 | Annotation score: | ||
F5H4U1 | F5H4U1_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 25 | Annotation score: | ||
H0YFL0 | H0YFL0_HUMAN | Cation-dependent mannose-6-phosphat... | M6PR | 96 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16985 mRNA Translation: AAA59542.1 X56254 , X56255, X56256, X56257 Genomic DNA Translation: CAB94715.1 AK291143 mRNA Translation: BAF83832.1 CH471116 Genomic DNA Translation: EAW88596.1 CH471116 Genomic DNA Translation: EAW88597.1 CH471116 Genomic DNA Translation: EAW88598.1 CH471116 Genomic DNA Translation: EAW88599.1 BC024206 mRNA Translation: AAH24206.1 |
CCDSi | CCDS8598.1 |
PIRi | S15368, A32700 |
RefSeqi | NP_002346.1, NM_002355.3 XP_005253433.1, XM_005253376.2 |
Genome annotation databases
Ensembli | ENST00000000412; ENSP00000000412; ENSG00000003056 |
GeneIDi | 4074 |
KEGGi | hsa:4074 |
UCSCi | uc001qvf.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M16985 mRNA Translation: AAA59542.1 X56254 , X56255, X56256, X56257 Genomic DNA Translation: CAB94715.1 AK291143 mRNA Translation: BAF83832.1 CH471116 Genomic DNA Translation: EAW88596.1 CH471116 Genomic DNA Translation: EAW88597.1 CH471116 Genomic DNA Translation: EAW88598.1 CH471116 Genomic DNA Translation: EAW88599.1 BC024206 mRNA Translation: AAH24206.1 |
CCDSi | CCDS8598.1 |
PIRi | S15368, A32700 |
RefSeqi | NP_002346.1, NM_002355.3 XP_005253433.1, XM_005253376.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JUQ | X-ray | 2.20 | E/F/G/H | 265-277 | [»] | |
SMRi | P20645 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110251, 41 interactors |
ELMi | P20645 |
IntActi | P20645, 16 interactors |
MINTi | P20645 |
STRINGi | 9606.ENSP00000000412 |
Chemistry databases
BindingDBi | P20645 |
ChEMBLi | CHEMBL5788 |
DrugBanki | DB02755, 1-3 Sugar Ring of Pentamannosyl 6-Phosphate DB01272, Alglucosidase alfa DB02900, alpha-D-mannose 6-phosphate |
Protein family/group databases
TCDBi | 9.B.247.1.1, the mannose 6-phosphate receptor (m6pr) family |
PTM databases
GlyConnecti | 1083, 20 N-Linked glycans (2 sites) |
GlyGeni | P20645, 6 sites, 1 N-linked glycan (1 site) |
iPTMneti | P20645 |
MetOSitei | P20645 |
PhosphoSitePlusi | P20645 |
SwissPalmi | P20645 |
Genetic variation databases
BioMutai | M6PR |
DMDMi | 127293 |
Proteomic databases
CPTACi | CPTAC-1308 |
EPDi | P20645 |
jPOSTi | P20645 |
MassIVEi | P20645 |
MaxQBi | P20645 |
PaxDbi | P20645 |
PeptideAtlasi | P20645 |
PRIDEi | P20645 |
ProteomicsDBi | 53769 |
Protocols and materials databases
Antibodypediai | 11573, 254 antibodies |
DNASUi | 4074 |
Genome annotation databases
Ensembli | ENST00000000412; ENSP00000000412; ENSG00000003056 |
GeneIDi | 4074 |
KEGGi | hsa:4074 |
UCSCi | uc001qvf.4, human |
Organism-specific databases
CTDi | 4074 |
DisGeNETi | 4074 |
GeneCardsi | M6PR |
HGNCi | HGNC:6752, M6PR |
HPAi | ENSG00000003056, Low tissue specificity |
MIMi | 154540, gene |
neXtProti | NX_P20645 |
OpenTargetsi | ENSG00000003056 |
PharmGKBi | PA30513 |
VEuPathDBi | HostDB:ENSG00000003056.7 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QTJ5, Eukaryota |
GeneTreei | ENSGT00390000002109 |
HOGENOMi | CLU_058440_0_0_1 |
InParanoidi | P20645 |
OMAi | RVCREVN |
OrthoDBi | 1214298at2759 |
PhylomeDBi | P20645 |
TreeFami | TF328910 |
Enzyme and pathway databases
PathwayCommonsi | P20645 |
Reactomei | R-HSA-432720, Lysosome Vesicle Biogenesis R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis |
SIGNORi | P20645 |
Miscellaneous databases
BioGRID-ORCSi | 4074, 8 hits in 875 CRISPR screens |
ChiTaRSi | M6PR, human |
GeneWikii | Cation-dependent_mannose-6-phosphate_receptor |
GenomeRNAii | 4074 |
Pharosi | P20645, Tbio |
PROi | PR:P20645 |
RNActi | P20645, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000003056, Expressed in small intestine Peyer's patch and 245 other tissues |
ExpressionAtlasi | P20645, baseline and differential |
Genevisiblei | P20645, HS |
Family and domain databases
Gene3Di | 2.70.130.10, 1 hit |
InterProi | View protein in InterPro IPR028927, Man-6-P_rcpt IPR000296, Man-6-P_rcpt_cation_dep IPR009011, Man6P_isomerase_rcpt-bd_dom_sf |
Pfami | View protein in Pfam PF02157, Man-6-P_recep, 1 hit |
PRINTSi | PR00715, MAN6PRECEPTR |
PROSITEi | View protein in PROSITE PS51914, MRH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MPRD_HUMAN | |
Accessioni | P20645Primary (citable) accession number: P20645 Secondary accession number(s): A8K528, D3DUV5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | February 10, 2021 | |
This is version 195 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references