UniProtKB - P20622 (NIFK_METTL)
Protein
Nitrogenase molybdenum-iron protein beta chain
Gene
nifK
Organism
Methanothermococcus thermolithotrophicus (Methanococcus thermolithotrophicus)
Status
Functioni
This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation.
Catalytic activityi
- 16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H+ + H2 + 2 NH4+ + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphateEC:1.18.6.1
Cofactori
[8Fe-7S] clusterBy similarityNote: Binds 1 [8Fe-7S] cluster per heterodimer.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 26 | Iron-sulfur (8Fe-7S); shared with alpha chainBy similarity | 1 | |
Metal bindingi | 51 | Iron-sulfur (8Fe-7S); shared with alpha chainBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- carbonyl sulfide nitrogenase activity Source: UniProtKB-EC
- iron-sulfur cluster binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- nitrogenase activity Source: UniProtKB-EC
GO - Biological processi
- nitrogen fixation Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Nitrogen fixation |
Ligand | ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Nitrogenase molybdenum-iron protein beta chain (EC:1.18.6.1)Alternative name(s): Dinitrogenase Nitrogenase component I |
Gene namesi | Name:nifK |
Organismi | Methanothermococcus thermolithotrophicus (Methanococcus thermolithotrophicus) |
Taxonomic identifieri | 2186 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Methanomada group › Methanococci › Methanococcales › Methanococcaceae › Methanothermococcus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153106 | 1 – ›79 | Nitrogenase molybdenum-iron protein beta chainAdd BLAST | ›79 |
Interactioni
Subunit structurei
Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2).
Family & Domainsi
Sequence similaritiesi
Belongs to the NifD/NifK/NifE/NifN family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
i Sequence
Sequence statusi: Fragment.
P20622-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSEVNAGEIC YVKKQRKGTI NPNKICQPIG AMWATVGVKG TIPFVQGSQG
60 70
CTTYVRYAFN RHFREPVSIA TASFHEHAA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 79 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13830 Genomic DNA Translation: CAA32059.1 |
PIRi | S06988 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13830 Genomic DNA Translation: CAA32059.1 |
PIRi | S06988 |
3D structure databases
SMRi | P20622 |
ModBasei | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR000510, Nase/OxRdtase_comp1 |
Pfami | View protein in Pfam PF00148, Oxidored_nitro, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NIFK_METTL | |
Accessioni | P20622Primary (citable) accession number: P20622 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | December 11, 2019 | |
This is version 72 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |