Gnat1

Functionⁱ

Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (By similarity). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	43	MagnesiumBy similarity	1
Binding siteⁱ	146	GTPBy similarity	1
Metal bindingⁱ	177	MagnesiumBy similarity	1
Binding siteⁱ	199	GTP; via amide nitrogenBy similarity	1
Binding siteⁱ	322	GTP; via amide nitrogenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	36 – 43	GTPBy similarity	8
Nucleotide bindingⁱ	171 – 177	GTPBy similarity	7
Nucleotide bindingⁱ	265 – 268	GTPBy similarity	4

GO - Molecular functionⁱ

G-protein beta/gamma-subunit complex binding
Source: MGI
Inferred from direct assayⁱ
- 17881533
G protein-coupled receptor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
GTPase activity
Source: MGI
Inferred from direct assayⁱ
- 17881533
GTP binding Source: MGI
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
cell proliferation
Source: MGI
Inferred from direct assayⁱ
- 14712229
cellular response to electrical stimulus
Source: MGI
Inferred from mutant phenotypeⁱ
- 21052544
detection of chemical stimulus involved in sensory perception of bitter taste Source: MGI
detection of light stimulus involved in visual perception
Source: MGI
Inferred from mutant phenotypeⁱ
- 18171936
eye photoreceptor cell development
Source: MGI
Inferred from mutant phenotypeⁱ
- 16249514
G protein-coupled receptor signaling pathway
Source: MGI
Inferred from direct assayⁱ
- 14712229
G protein-coupled receptor signaling pathway coupled to cGMP nucleotide second messenger
Source: MGI
Traceable author statementⁱ
- 2708360
phototransduction
Source: MGI
Inferred from mutant phenotypeⁱ
phototransduction, visible light Source: MGI
positive regulation of cyclic-nucleotide phosphodiesterase activity Source: MGI
response to light intensity Source: MGI
retina development in camera-type eye
Source: MGI
Inferred from mutant phenotypeⁱ
- 21052544
rhodopsin mediated signaling pathway Source: MGI
sensory perception of umami taste
Source: MGI
Inferred from mutant phenotypeⁱ
- 15342734
visual perception
Source: MGI
Inferred from mutant phenotypeⁱ
- 16603466

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transducer
Biological process	Sensory transduction, Vision
Ligand	GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-112043 PLC beta mediated events R-MMU-202040 G-protein activation R-MMU-2485179 Activation of the phototransduction cascade R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade R-MMU-418594 G alpha (i) signalling events R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Reactomeⁱ

R-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Guanine nucleotide-binding protein G(t) subunit alpha-1 Alternative name(s): Transducin alpha-1 chain
Gene namesⁱ	Name:Gnat1 Synonyms:Gnat-1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 9

Organism-specific databases

MGIⁱ	MGI:95778 Gnat1

Keywords - Cellular componentⁱ

Cell projection, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedBy similarity
ChainⁱPRO_0000203738	2 – 350	Guanine nucleotide-binding protein G(t) subunit alpha-1Add BLAST		349

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Lipidationⁱ	2	N-myristoyl glycineBy similarity		1
Modified residueⁱ	142	PhosphotyrosineBy similarity		1

Keywords - PTMⁱ

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P20612
PaxDbⁱ	P20612
PRIDEⁱ	P20612

PTM databases

iPTMnetⁱ	P20612
PhosphoSitePlusⁱ	P20612

Expressionⁱ

Tissue specificityⁱ

Rod.

Gene expression databases

Bgeeⁱ	ENSMUSG00000034837 Expressed in 43 organ(s), highest expression level in retina
ExpressionAtlasⁱ	P20612 baseline and differential
Genevisibleⁱ	P20612 MM

Interactionⁱ

Subunit structureⁱ

Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RHO. Interacts with RGS9 and PDE6G (By similarity). Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).By similarity

GO - Molecular functionⁱ

G protein-coupled receptor binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000010205

STRINGⁱ

10090.ENSMUSP00000010205

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P20612
SMRⁱ	P20612
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	340 – 350	Interaction with RHOBy similarityAdd BLAST		11

Sequence similaritiesⁱ

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGⁱ	KOG0082 Eukaryota ENOG410XNVQ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000118851
HOGENOMⁱ	HOG000038730
HOVERGENⁱ	HBG063184
InParanoidⁱ	P20612
KEGG Orthology (KO) More...KOⁱ	K04631
OMAⁱ	MHLADTI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0VUT
PhylomeDBⁱ	P20612
TreeFamⁱ	TF300673

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00066 G-alpha, 1 hit
Gene3Dⁱ	1.10.400.10, 1 hit
InterProⁱ	View protein in InterPro IPR001408 Gprotein_alpha_I IPR001019 Gprotein_alpha_su IPR011025 GproteinA_insert IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR10218 PTHR10218, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00503 G-alpha, 1 hit
PRINTSⁱ	PR00318 GPROTEINA PR00441 GPROTEINAI
SMARTⁱ	View protein in SMART SM00275 G_alpha, 1 hit
SUPFAMⁱ	SSF47895 SSF47895, 1 hit SSF52540 SSF52540, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P20612-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK 
        60         70         80         90        100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA 
       110        120        130        140        150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY 
       160        170        180        190        200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ 
       210        220        230        240        250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC 
       260        270        280        290        300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK 
       310        320        330        340        350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF

Length:350

Mass (Da):39,967

Last modified:January 23, 2007 - v3

Checksum:ⁱ3810BA8DEEFBD7D5

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0A6YWJ0	A0A0A6YWJ0_MOUSE		Guanine nucleotide-binding protein ... Guanine nucleotide-binding protein G(t) subunit alpha-1	Gnat1	87	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M25513 M25512 Genomic DNA Translation: AAA40473.1 BC022793 mRNA Translation: AAH22793.1 BC051412 mRNA Translation: AAH51412.2 BC058810 mRNA Translation: AAH58810.1 U38504 mRNA Translation: AAB01735.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS23504.1
PIRⁱ	A33352 RGMST1
NCBI Reference Sequences More...RefSeqⁱ	NP_032166.1, NM_008140.2
UniGeneⁱ	Mm.284853

Genome annotation databases

Ensemblⁱ	ENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837
GeneIDⁱ	14685
KEGGⁱ	mmu:14685
UCSC genome browser More...UCSCⁱ	uc009rmr.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P20612	Uncharacterized protein		PROCO		350	UniRef100_P20612
Uncharacterized protein		OTOGA		350

Protein	Similar proteins		Species	Score	Length	Source
P20612	Guanine nucleotide-binding protein G(t) subunit alpha-1	)	HUMAN		350	UniRef90_P11488
Guanine nucleotide-binding protein G(t) subunit alpha-1		CANLF		350
G protein subunit alpha transducin 1		PANTR		350
G protein subunit alpha transducin 1		MACFA		350
GNAT1 isoform 1		PONAB		350
+66

Protein	Similar proteins		Species	Score	Length	Source
P20612	Guanine nucleotide-binding protein G(t) subunit alpha-1	)	HUMAN		350	UniRef50_P11488
Guanine nucleotide-binding protein G(t) subunit alpha-1		CANLF		350
G protein subunit alpha transducin 1		PANTR		350
G protein subunit alpha transducin 1		MACFA		350
GNAT1 isoform 1		PONAB		350
+68

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M25513 M25512 Genomic DNA Translation: AAA40473.1 BC022793 mRNA Translation: AAH22793.1 BC051412 mRNA Translation: AAH51412.2 BC058810 mRNA Translation: AAH58810.1 U38504 mRNA Translation: AAB01735.1
CCDSⁱ	CCDS23504.1
PIRⁱ	A33352 RGMST1
RefSeqⁱ	NP_032166.1, NM_008140.2
UniGeneⁱ	Mm.284853

3D structure databases

ProteinModelPortalⁱ	P20612
SMRⁱ	P20612
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000010205

STRINGⁱ

10090.ENSMUSP00000010205

PTM databases

iPTMnetⁱ	P20612
PhosphoSitePlusⁱ	P20612

Proteomic databases

MaxQBⁱ	P20612
PaxDbⁱ	P20612
PRIDEⁱ	P20612

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837
GeneIDⁱ	14685
KEGGⁱ	mmu:14685
UCSCⁱ	uc009rmr.1 mouse

Organism-specific databases

CTDⁱ	2779
MGIⁱ	MGI:95778 Gnat1

Phylogenomic databases

eggNOGⁱ	KOG0082 Eukaryota ENOG410XNVQ LUCA
GeneTreeⁱ	ENSGT00760000118851
HOGENOMⁱ	HOG000038730
HOVERGENⁱ	HBG063184
InParanoidⁱ	P20612
KOⁱ	K04631
OMAⁱ	MHLADTI
OrthoDBⁱ	EOG091G0VUT
PhylomeDBⁱ	P20612
TreeFamⁱ	TF300673

Enzyme and pathway databases

Reactomeⁱ	R-MMU-112043 PLC beta mediated events R-MMU-202040 G-protein activation R-MMU-2485179 Activation of the phototransduction cascade R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade R-MMU-418594 G alpha (i) signalling events R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Reactomeⁱ

R-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Miscellaneous databases

ChiTaRSⁱ	Gnat1 mouse
Protein Ontology More...PROⁱ	PR:P20612
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000034837 Expressed in 43 organ(s), highest expression level in retina
ExpressionAtlasⁱ	P20612 baseline and differential
Genevisibleⁱ	P20612 MM

Family and domain databases

CDDⁱ	cd00066 G-alpha, 1 hit
Gene3Dⁱ	1.10.400.10, 1 hit
InterProⁱ	View protein in InterPro IPR001408 Gprotein_alpha_I IPR001019 Gprotein_alpha_su IPR011025 GproteinA_insert IPR027417 P-loop_NTPase
PANTHERⁱ	PTHR10218 PTHR10218, 1 hit
Pfamⁱ	View protein in Pfam PF00503 G-alpha, 1 hit
PRINTSⁱ	PR00318 GPROTEINA PR00441 GPROTEINAI
SMARTⁱ	View protein in SMART SM00275 G_alpha, 1 hit
SUPFAMⁱ	SSF47895 SSF47895, 1 hit SSF52540 SSF52540, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	GNAT1_MOUSE
Accessionⁱ	P20612Primary (citable) accession number: P20612 Secondary accession number(s): Q80X34
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P20612 (GNAT1_MOUSE)

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Guanine nucleotide-binding protein G(t) subunit alpha-1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Plasma Membrane

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ