Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein G(t) subunit alpha-1

Gene

Gnat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (By similarity). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43MagnesiumBy similarity1
Binding sitei146GTPBy similarity1
Metal bindingi177MagnesiumBy similarity1
Binding sitei199GTP; via amide nitrogenBy similarity1
Binding sitei322GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43GTPBy similarity8
Nucleotide bindingi171 – 177GTPBy similarity7
Nucleotide bindingi265 – 268GTPBy similarity4

GO - Molecular functioni

  • G-protein beta/gamma-subunit complex binding Source: MGI
  • G protein-coupled receptor binding Source: GO_Central
  • GTPase activity Source: MGI
  • GTP binding Source: MGI
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionTransducer
Biological processSensory transduction, Vision
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(t) subunit alpha-1
Alternative name(s):
Transducin alpha-1 chain
Gene namesi
Name:Gnat1
Synonyms:Gnat-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95778 Gnat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002037382 – 350Guanine nucleotide-binding protein G(t) subunit alpha-1Add BLAST349

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei142PhosphotyrosineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

MaxQBiP20612
PaxDbiP20612
PRIDEiP20612

PTM databases

iPTMnetiP20612
PhosphoSitePlusiP20612

Expressioni

Tissue specificityi

Rod.

Gene expression databases

BgeeiENSMUSG00000034837 Expressed in 43 organ(s), highest expression level in retina
ExpressionAtlasiP20612 baseline and differential
GenevisibleiP20612 MM

Interactioni

Subunit structurei

Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with RHO. Interacts with RGS9 and PDE6G (By similarity). Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205

Structurei

3D structure databases

ProteinModelPortaliP20612
SMRiP20612
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni340 – 350Interaction with RHOBy similarityAdd BLAST11

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP20612
KOiK04631
OMAiMHLADTI
OrthoDBiEOG091G0VUT
PhylomeDBiP20612
TreeFamiTF300673

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P20612-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Length:350
Mass (Da):39,967
Last modified:January 23, 2007 - v3
Checksum:i3810BA8DEEFBD7D5
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A6YWJ0A0A0A6YWJ0_MOUSE
Guanine nucleotide-binding protein ...
Gnat1
87Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA Translation: AAA40473.1
BC022793 mRNA Translation: AAH22793.1
BC051412 mRNA Translation: AAH51412.2
BC058810 mRNA Translation: AAH58810.1
U38504 mRNA Translation: AAB01735.1
CCDSiCCDS23504.1
PIRiA33352 RGMST1
RefSeqiNP_032166.1, NM_008140.2
UniGeneiMm.284853

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837
GeneIDi14685
KEGGimmu:14685
UCSCiuc009rmr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25513
, M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA Translation: AAA40473.1
BC022793 mRNA Translation: AAH22793.1
BC051412 mRNA Translation: AAH51412.2
BC058810 mRNA Translation: AAH58810.1
U38504 mRNA Translation: AAB01735.1
CCDSiCCDS23504.1
PIRiA33352 RGMST1
RefSeqiNP_032166.1, NM_008140.2
UniGeneiMm.284853

3D structure databases

ProteinModelPortaliP20612
SMRiP20612
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010205

PTM databases

iPTMnetiP20612
PhosphoSitePlusiP20612

Proteomic databases

MaxQBiP20612
PaxDbiP20612
PRIDEiP20612

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837
GeneIDi14685
KEGGimmu:14685
UCSCiuc009rmr.1 mouse

Organism-specific databases

CTDi2779
MGIiMGI:95778 Gnat1

Phylogenomic databases

eggNOGiKOG0082 Eukaryota
ENOG410XNVQ LUCA
GeneTreeiENSGT00760000118851
HOGENOMiHOG000038730
HOVERGENiHBG063184
InParanoidiP20612
KOiK04631
OMAiMHLADTI
OrthoDBiEOG091G0VUT
PhylomeDBiP20612
TreeFamiTF300673

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-202040 G-protein activation
R-MMU-2485179 Activation of the phototransduction cascade
R-MMU-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-MMU-418594 G alpha (i) signalling events
R-MMU-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding

Miscellaneous databases

ChiTaRSiGnat1 mouse
PROiPR:P20612
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034837 Expressed in 43 organ(s), highest expression level in retina
ExpressionAtlasiP20612 baseline and differential
GenevisibleiP20612 MM

Family and domain databases

CDDicd00066 G-alpha, 1 hit
Gene3Di1.10.400.10, 1 hit
InterProiView protein in InterPro
IPR001408 Gprotein_alpha_I
IPR001019 Gprotein_alpha_su
IPR011025 GproteinA_insert
IPR027417 P-loop_NTPase
PANTHERiPTHR10218 PTHR10218, 1 hit
PfamiView protein in Pfam
PF00503 G-alpha, 1 hit
PRINTSiPR00318 GPROTEINA
PR00441 GPROTEINAI
SMARTiView protein in SMART
SM00275 G_alpha, 1 hit
SUPFAMiSSF47895 SSF47895, 1 hit
SSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGNAT1_MOUSE
AccessioniPrimary (citable) accession number: P20612
Secondary accession number(s): Q80X34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 168 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again