UniProtKB - P20612 (GNAT1_MOUSE)
Protein
Guanine nucleotide-binding protein G(t) subunit alpha-1
Gene
Gnat1
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (By similarity). Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 43 | MagnesiumBy similarity | 1 | |
Binding sitei | 146 | GTPBy similarity | 1 | |
Metal bindingi | 177 | MagnesiumBy similarity | 1 | |
Binding sitei | 199 | GTP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 322 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 36 – 43 | GTPBy similarity | 8 | |
Nucleotide bindingi | 171 – 177 | GTPBy similarity | 7 | |
Nucleotide bindingi | 265 – 268 | GTPBy similarity | 4 |
GO - Molecular functioni
- G-protein beta/gamma-subunit complex binding Source: MGI
- G protein-coupled receptor binding Source: GO_Central
- GTPase activity Source: MGI
- GTP binding Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: GO_Central
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cell population proliferation Source: MGI
- cellular response to electrical stimulus Source: MGI
- detection of chemical stimulus involved in sensory perception of bitter taste Source: MGI
- detection of light stimulus involved in visual perception Source: MGI
- eye photoreceptor cell development Source: MGI
- G protein-coupled adenosine receptor signaling pathway Source: GO_Central
- G protein-coupled receptor signaling pathway Source: MGI
- G protein-coupled receptor signaling pathway coupled to cGMP nucleotide second messenger Source: MGI
- phototransduction Source: MGI
- phototransduction, visible light Source: MGI
- positive regulation of cyclic-nucleotide phosphodiesterase activity Source: MGI
- response to light intensity Source: MGI
- retina development in camera-type eye Source: MGI
- rhodopsin mediated signaling pathway Source: MGI
- sensory perception of umami taste Source: MGI
- visual perception Source: MGI
Keywordsi
Molecular function | Transducer |
Biological process | Sensory transduction, Vision |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-112043, PLC beta mediated events R-MMU-202040, G-protein activation R-MMU-2485179, Activation of the phototransduction cascade R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-MMU-418594, G alpha (i) signalling events R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(t) subunit alpha-1Alternative name(s): Transducin alpha-1 chain |
Gene namesi | Name:Gnat1 Synonyms:Gnat-1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:95778, Gnat1 |
Subcellular locationi
Other locations
- photoreceptor outer segment 1 Publication
- Membrane By similarity; Peripheral membrane protein By similarity
- Photoreceptor inner segment 1 Publication
Note: Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.1 Publication
Plasma Membrane
- apical plasma membrane Source: MGI
- heterotrimeric G-protein complex Source: MGI
Other locations
- cytoplasm Source: MGI
- membrane Source: MGI
- neuronal cell body Source: MGI
- photoreceptor connecting cilium Source: MGI
- photoreceptor inner segment Source: UniProtKB
- photoreceptor outer segment Source: UniProtKB
Keywords - Cellular componenti
Cell projection, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000203738 | 2 – 350 | Guanine nucleotide-binding protein G(t) subunit alpha-1Add BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycineBy similarity | 1 | |
Modified residuei | 142 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Lipoprotein, Myristate, PhosphoproteinProteomic databases
jPOSTi | P20612 |
MaxQBi | P20612 |
PaxDbi | P20612 |
PRIDEi | P20612 |
PTM databases
iPTMneti | P20612 |
PhosphoSitePlusi | P20612 |
Expressioni
Tissue specificityi
In the retina, expressed in the rod photoreceptors.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000034837, Expressed in retina and 79 other tissues |
ExpressionAtlasi | P20612, baseline and differential |
Genevisiblei | P20612, MM |
Interactioni
Subunit structurei
Heterotrimeric G proteins are composed of 3 subunits alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Interacts with RHO.
Interacts with RGS9 and PDE6G (By similarity).
Interacts (when myristoylated) with UNC119; interaction is required for localization in sensory neurons (By similarity).
By similarityGO - Molecular functioni
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 199973, 2 interactors |
IntActi | P20612, 1 interactor |
STRINGi | 10090.ENSMUSP00000010205 |
Miscellaneous databases
RNActi | P20612, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 28 – 350 | G-alphaPROSITE-ProRule annotationAdd BLAST | 323 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 31 – 44 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 169 – 177 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 192 – 201 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 261 – 268 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 320 – 325 | G5 motifPROSITE-ProRule annotation | 6 | |
Regioni | 340 – 350 | Interaction with RHOBy similarityAdd BLAST | 11 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000160395 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P20612 |
OMAi | ECLEFIV |
OrthoDBi | 754573at2759 |
PhylomeDBi | P20612 |
TreeFami | TF300673 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P20612-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK
60 70 80 90 100
IIHQDGYSLE ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA
110 120 130 140 150
RKLMHMADTI EEGTMPKEMS DIIQRLWKDS GIQACFDRAS EYQLNDSAGY
160 170 180 190 200
YLSDLERLVT PGYVPTEQDV LRSRVKTTGI IETQFSFKDL NFRMFDVGGQ
210 220 230 240 250
RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH ESLHLFNSIC
260 270 280 290 300
NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0A6YWJ0 | A0A0A6YWJ0_MOUSE | Guanine nucleotide-binding protein ... | Gnat1 | 87 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25513 , M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA Translation: AAA40473.1 BC022793 mRNA Translation: AAH22793.1 BC051412 mRNA Translation: AAH51412.2 BC058810 mRNA Translation: AAH58810.1 U38504 mRNA Translation: AAB01735.1 |
CCDSi | CCDS23504.1 |
PIRi | A33352, RGMST1 |
RefSeqi | NP_032166.1, NM_008140.2 |
Genome annotation databases
Ensembli | ENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837 |
GeneIDi | 14685 |
KEGGi | mmu:14685 |
UCSCi | uc009rmr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25513 , M25506, M25507, M25508, M25509, M25510, M25511, M25512 Genomic DNA Translation: AAA40473.1 BC022793 mRNA Translation: AAH22793.1 BC051412 mRNA Translation: AAH51412.2 BC058810 mRNA Translation: AAH58810.1 U38504 mRNA Translation: AAB01735.1 |
CCDSi | CCDS23504.1 |
PIRi | A33352, RGMST1 |
RefSeqi | NP_032166.1, NM_008140.2 |
3D structure databases
SMRi | P20612 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199973, 2 interactors |
IntActi | P20612, 1 interactor |
STRINGi | 10090.ENSMUSP00000010205 |
PTM databases
iPTMneti | P20612 |
PhosphoSitePlusi | P20612 |
Proteomic databases
jPOSTi | P20612 |
MaxQBi | P20612 |
PaxDbi | P20612 |
PRIDEi | P20612 |
Protocols and materials databases
Antibodypediai | 13896, 179 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000010205; ENSMUSP00000010205; ENSMUSG00000034837 |
GeneIDi | 14685 |
KEGGi | mmu:14685 |
UCSCi | uc009rmr.1, mouse |
Organism-specific databases
CTDi | 2779 |
MGIi | MGI:95778, Gnat1 |
Phylogenomic databases
eggNOGi | KOG0082, Eukaryota |
GeneTreei | ENSGT00940000160395 |
HOGENOMi | CLU_014184_6_0_1 |
InParanoidi | P20612 |
OMAi | ECLEFIV |
OrthoDBi | 754573at2759 |
PhylomeDBi | P20612 |
TreeFami | TF300673 |
Enzyme and pathway databases
Reactomei | R-MMU-112043, PLC beta mediated events R-MMU-202040, G-protein activation R-MMU-2485179, Activation of the phototransduction cascade R-MMU-2514859, Inactivation, recovery and regulation of the phototransduction cascade R-MMU-418594, G alpha (i) signalling events R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Miscellaneous databases
BioGRID-ORCSi | 14685, 0 hits in 17 CRISPR screens |
ChiTaRSi | Gnat1, mouse |
PROi | PR:P20612 |
RNActi | P20612, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000034837, Expressed in retina and 79 other tissues |
ExpressionAtlasi | P20612, baseline and differential |
Genevisiblei | P20612, MM |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit |
InterProi | View protein in InterPro IPR001408, Gprotein_alpha_I IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00441, GPROTEINAI |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAT1_MOUSE | |
Accessioni | P20612Primary (citable) accession number: P20612 Secondary accession number(s): Q80X34 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families