UniProtKB - P20591 (MX1_HUMAN)
Interferon-induced GTP-binding protein Mx1
MX1
Functioni
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 77 – 84 | GTPSequence analysis | 8 | |
Nucleotide bindingi | 178 – 182 | GTPSequence analysis | 5 | |
Nucleotide bindingi | 247 – 250 | GTPSequence analysis | 4 |
GO - Molecular functioni
- GTPase activity Source: GO_Central
- GTP binding Source: ProtInc
- identical protein binding Source: IntAct
- microtubule binding Source: GO_Central
GO - Biological processi
- apoptotic process Source: ProtInc
- defense response Source: ProtInc
- defense response to virus Source: UniProtKB
- dynamin family protein polymerization involved in mitochondrial fission Source: GO_Central
- innate immune response Source: UniProtKB
- membrane fusion Source: GO_Central
- mitochondrial fission Source: GO_Central
- negative regulation of viral genome replication Source: UniProtKB
- organelle fission Source: GO_Central
- postsynaptic neurotransmitter receptor internalization Source: GO_Central
- receptor internalization Source: GO_Central
- regulation of synapse structure or activity Source: GO_Central
- response to type I interferon Source: UniProtKB
- response to virus Source: UniProtKB
- signal transduction Source: ProtInc
- synaptic vesicle budding from presynaptic endocytic zone membrane Source: GO_Central
- type I interferon signaling pathway Source: Reactome
Keywordsi
Biological process | Antiviral defense, Immunity, Innate immunity |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P20591 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-909733, Interferon alpha/beta signaling |
SIGNORi | P20591 |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon-induced GTP-binding protein Mx1Alternative name(s): Interferon-induced protein p78 Short name: IFI-78K Interferon-regulated resistance GTP-binding protein MxA Myxoma resistance protein 1 Myxovirus resistance protein 1 Cleaved into the following chain: |
Gene namesi | Name:MX1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000157601.13 |
HGNCi | HGNC:7532, MX1 |
MIMi | 147150, gene |
neXtProti | NX_P20591 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 3 Publications; Peripheral membrane protein 1 Publication; Cytoplasmic side
Other locations
- Cytoplasm 4 Publications
- perinuclear region 1 Publication
Note: Binds preferentially to negatively charged phospholipids (PubMed:21900240). Colocalizes with CCHFV protein N in the perinuclear region (PubMed:15047845).2 Publications
Cytoskeleton
- microtubule cytoskeleton Source: GO_Central
Cytosol
- cytosol Source: HPA
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial membrane Source: GO_Central
Nucleus
- nuclear membrane Source: HPA
- nucleus Source: GO_Central
Plasma Membrane
- plasma membrane Source: GO_Central
- postsynaptic endocytic zone membrane Source: GO_Central
- postsynaptic membrane Source: GO_Central
Other locations
- axon Source: GO_Central
- cytoplasm Source: UniProtKB
- cytoplasmic vesicle Source: GO_Central
- dendritic spine Source: GO_Central
- dendritic spine head Source: GO_Central
- membrane Source: GO_Central
- perinuclear region of cytoplasm Source: UniProtKB
- postsynaptic density Source: GO_Central
- presynapse Source: GOC
- synapse Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 81 | S → C: No effect on GTP-binding, nor on viral infection. 1 Publication | 1 | |
Mutagenesisi | 83 | K → A: Loss of GTP-binding. Loss of potentiation of TRPC6 activity. Loss of protection against viral infection. 2 Publications | 1 | |
Mutagenesisi | 83 | K → M: Loss of GTP-binding. Loss of protection against viral infection. 2 Publications | 1 | |
Mutagenesisi | 103 | T → A: Loss of GTP-binding. Loss of potentiation of TRPC6 activity. Loss of protection against viral infection. 2 Publications | 1 | |
Mutagenesisi | 554 | K → E: Strong liposome-binding reduction. 1 Publication | 1 | |
Mutagenesisi | 555 | K → E: Strong liposome-binding reduction. 1 Publication | 1 | |
Mutagenesisi | 556 | K → E: Strong liposome-binding reduction. 1 Publication | 1 | |
Mutagenesisi | 557 | K → E: Strong liposome-binding reduction. 1 Publication | 1 | |
Mutagenesisi | 612 | L → K: Loss of GTP-hydrolysis. No effect on GTP-binding, nor on potentiation of TRPC6 activity. 1 Publication | 1 | |
Mutagenesisi | 632 | E → A: Reduced antiviral activity. 1 Publication | 1 | |
Mutagenesisi | 640 | R → A: Fails to sequester viral nucleoproteins, no antiviral activity. 1 Publication | 1 | |
Mutagenesisi | 645 | E → R: Loss of antiviral activity towards CCHFV and LACV. 2 Publications | 1 |
Organism-specific databases
DisGeNETi | 4599 |
OpenTargetsi | ENSG00000157601 |
PharmGKBi | PA31333 |
Miscellaneous databases
Pharosi | P20591, Tbio |
Polymorphism and mutation databases
BioMutai | MX1 |
DMDMi | 251757499 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000382943 | 1 – 662 | Interferon-induced GTP-binding protein Mx1Add BLAST | 662 | |
Initiator methioninei | Removed; alternate2 Publications | |||
ChainiPRO_0000206592 | 2 – 662 | Interferon-induced GTP-binding protein Mx1, N-terminally processedAdd BLAST | 661 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine; in Interferon-induced GTP-binding protein Mx1; alternate1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
EPDi | P20591 |
jPOSTi | P20591 |
MassIVEi | P20591 |
MaxQBi | P20591 |
PaxDbi | P20591 |
PeptideAtlasi | P20591 |
PRIDEi | P20591 |
ProteomicsDBi | 53763 [P20591-1] 53764 [P20591-2] |
PTM databases
iPTMneti | P20591 |
PhosphoSitePlusi | P20591 |
SwissPalmi | P20591 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000157601, Expressed in epithelium of bronchus and 234 other tissues |
ExpressionAtlasi | P20591, baseline and differential |
Genevisiblei | P20591, HS |
Organism-specific databases
HPAi | ENSG00000157601, Low tissue specificity |
Interactioni
Subunit structurei
Homotetramer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures.
Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7.
Interacts with HSPA5.
Interacts with DDX39A and DDX39B.
Interacts with TUBB/TUBB5 (By similarity). The GTP-bound form interacts (via C-terminus) with THOV P5 protein. The GTP-bound form interacts with LACV protein N.
Interacts with CCHFV protein N.
By similarity10 PublicationsBinary interactionsi
Hide detailsP20591
GO - Molecular functioni
- identical protein binding Source: IntAct
- microtubule binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 110684, 48 interactors |
DIPi | DIP-35694N |
IntActi | P20591, 21 interactors |
STRINGi | 9606.ENSP00000381601 |
Miscellaneous databases
RNActi | P20591, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P20591 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20591 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 67 – 340 | Dynamin-type GPROSITE-ProRule annotationAdd BLAST | 274 | |
Domaini | 574 – 662 | GEDPROSITE-ProRule annotationAdd BLAST | 89 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 77 – 84 | G1 motifPROSITE-ProRule annotation | 8 | |
Regioni | 102 – 104 | G2 motifPROSITE-ProRule annotation | 3 | |
Regioni | 178 – 181 | G3 motifPROSITE-ProRule annotation | 4 | |
Regioni | 247 – 250 | G4 motifPROSITE-ProRule annotation | 4 | |
Regioni | 279 – 282 | G5 motifPROSITE-ProRule annotation | 4 | |
Regioni | 341 – 366 | Bundle signaling element (BSE)Add BLAST | 26 | |
Regioni | 366 – 533 | Middle domainAdd BLAST | 168 | |
Regioni | 367 – 632 | StalkAdd BLAST | 266 | |
Regioni | 554 – 557 | Critical for lipid-binding | 4 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00940000155686 |
HOGENOMi | CLU_008964_8_0_1 |
InParanoidi | P20591 |
OMAi | EDEHEKM |
OrthoDBi | 494748at2759 |
PhylomeDBi | P20591 |
TreeFami | TF331484 |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
DisProti | DP01239 |
InterProi | View protein in InterPro IPR000375, Dynamin_central IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVVSEVDIAK ADPAAASHPL LLNGDATVAQ KNPGSVAENN LCSQYEEKVR
60 70 80 90 100
PCIDLIDSLR ALGVEQDLAL PAIAVIGDQS SGKSSVLEAL SGVALPRGSG
110 120 130 140 150
IVTRCPLVLK LKKLVNEDKW RGKVSYQDYE IEISDASEVE KEINKAQNAI
160 170 180 190 200
AGEGMGISHE LITLEISSRD VPDLTLIDLP GITRVAVGNQ PADIGYKIKT
210 220 230 240 250
LIKKYIQRQE TISLVVVPSN VDIATTEALS MAQEVDPEGD RTIGILTKPD
260 270 280 290 300
LVDKGTEDKV VDVVRNLVFH LKKGYMIVKC RGQQEIQDQL SLSEALQREK
310 320 330 340 350
IFFENHPYFR DLLEEGKATV PCLAEKLTSE LITHICKSLP LLENQIKETH
360 370 380 390 400
QRITEELQKY GVDIPEDENE KMFFLIDKVN AFNQDITALM QGEETVGEED
410 420 430 440 450
IRLFTRLRHE FHKWSTIIEN NFQEGHKILS RKIQKFENQY RGRELPGFVN
460 470 480 490 500
YRTFETIVKQ QIKALEEPAV DMLHTVTDMV RLAFTDVSIK NFEEFFNLHR
510 520 530 540 550
TAKSKIEDIR AEQEREGEKL IRLHFQMEQI VYCQDQVYRG ALQKVREKEL
560 570 580 590 600
EEEKKKKSWD FGAFQSSSAT DSSMEEIFQH LMAYHQEASK RISSHIPLII
610 620 630 640 650
QFFMLQTYGQ QLQKAMLQLL QDKDTYSWLL KERSDTSDKR KFLKERLARL
660
TQARRRLAQF PG
Computationally mapped potential isoform sequencesi
There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8W8T1 | F8W8T1_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 639 | Annotation score: | ||
H9KVC9 | H9KVC9_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 201 | Annotation score: | ||
H9KVC7 | H9KVC7_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 197 | Annotation score: | ||
H9KVC4 | H9KVC4_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 77 | Annotation score: | ||
H9KVD0 | H9KVD0_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 68 | Annotation score: | ||
H9KVD3 | H9KVD3_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 35 | Annotation score: | ||
H9KVC3 | H9KVC3_HUMAN | Interferon-induced GTP-binding prot... | MX1 | 25 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 164 | L → R in AAA36337 (PubMed:2481229).Curated | 1 | |
Sequence conflicti | 250 | D → G in BAG53272 (PubMed:20603636).Curated | 1 | |
Sequence conflicti | 297 | Q → H in BAG37852 (PubMed:20603636).Curated | 1 | |
Sequence conflicti | 299 | E → G in BAG53272 (PubMed:20603636).Curated | 1 | |
Sequence conflicti | 582 | M → I in BAG37852 (PubMed:20603636).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_058010 | 379 | V → I6 PublicationsCorresponds to variant dbSNP:rs469390Ensembl. | 1 | |
Natural variantiVAR_034116 | 381 | A → V. Corresponds to variant dbSNP:rs34717738Ensembl. | 1 | |
Natural variantiVAR_034117 | 611 | Q → H. Corresponds to variant dbSNP:rs2230454Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042904 | 425 – 662 | GHKIL…AQFPG → GGQQAHLQPHPFDHPVLHAP DVRPAASEGHAAAPAGQGHL QLAPEGAERHQRQAEVPEGA ACTADAGSAPACPVPRLTTL CPAP in isoform 2. 1 PublicationAdd BLAST | 238 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000398598; ENSP00000381599; ENSG00000157601 [P20591-1] ENST00000398600; ENSP00000381601; ENSG00000157601 [P20591-1] ENST00000455164; ENSP00000410523; ENSG00000157601 [P20591-1] ENST00000619682; ENSP00000478441; ENSG00000157601 [P20591-2] |
GeneIDi | 4599 |
KEGGi | hsa:4599 |
UCSCi | uc002yzh.5, human [P20591-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3LJB | X-ray | 2.40 | A/B | 366-636 | [»] | |
3SZR | X-ray | 3.50 | A | 33-662 | [»] | |
3ZYS | electron microscopy | 12.20 | B/E | 1-662 | [»] | |
4P4S | X-ray | 3.30 | A | 70-342 | [»] | |
B | 43-662 | [»] | ||||
4P4T | X-ray | 2.30 | A | 37-366 | [»] | |
A | 637-662 | [»] | ||||
4P4U | X-ray | 1.90 | A | 37-364 | [»] | |
A | 632-661 | [»] | ||||
5GTM | X-ray | 2.90 | A/B | 33-662 | [»] | |
SMRi | P20591 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 110684, 48 interactors |
DIPi | DIP-35694N |
IntActi | P20591, 21 interactors |
STRINGi | 9606.ENSP00000381601 |
PTM databases
iPTMneti | P20591 |
PhosphoSitePlusi | P20591 |
SwissPalmi | P20591 |
Polymorphism and mutation databases
BioMutai | MX1 |
DMDMi | 251757499 |
Proteomic databases
EPDi | P20591 |
jPOSTi | P20591 |
MassIVEi | P20591 |
MaxQBi | P20591 |
PaxDbi | P20591 |
PeptideAtlasi | P20591 |
PRIDEi | P20591 |
ProteomicsDBi | 53763 [P20591-1] 53764 [P20591-2] |
Protocols and materials databases
Antibodypediai | 23580, 478 antibodies |
DNASUi | 4599 |
Genome annotation databases
Ensembli | ENST00000398598; ENSP00000381599; ENSG00000157601 [P20591-1] ENST00000398600; ENSP00000381601; ENSG00000157601 [P20591-1] ENST00000455164; ENSP00000410523; ENSG00000157601 [P20591-1] ENST00000619682; ENSP00000478441; ENSG00000157601 [P20591-2] |
GeneIDi | 4599 |
KEGGi | hsa:4599 |
UCSCi | uc002yzh.5, human [P20591-1] |
Organism-specific databases
CTDi | 4599 |
DisGeNETi | 4599 |
EuPathDBi | HostDB:ENSG00000157601.13 |
GeneCardsi | MX1 |
HGNCi | HGNC:7532, MX1 |
HPAi | ENSG00000157601, Low tissue specificity |
MIMi | 147150, gene |
neXtProti | NX_P20591 |
OpenTargetsi | ENSG00000157601 |
PharmGKBi | PA31333 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0446, Eukaryota |
GeneTreei | ENSGT00940000155686 |
HOGENOMi | CLU_008964_8_0_1 |
InParanoidi | P20591 |
OMAi | EDEHEKM |
OrthoDBi | 494748at2759 |
PhylomeDBi | P20591 |
TreeFami | TF331484 |
Enzyme and pathway databases
PathwayCommonsi | P20591 |
Reactomei | R-HSA-1169408, ISG15 antiviral mechanism R-HSA-909733, Interferon alpha/beta signaling |
SIGNORi | P20591 |
Miscellaneous databases
BioGRID-ORCSi | 4599, 11 hits in 849 CRISPR screens |
ChiTaRSi | MX1, human |
EvolutionaryTracei | P20591 |
GeneWikii | MX1 |
GenomeRNAii | 4599 |
Pharosi | P20591, Tbio |
PROi | PR:P20591 |
RNActi | P20591, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000157601, Expressed in epithelium of bronchus and 234 other tissues |
ExpressionAtlasi | P20591, baseline and differential |
Genevisiblei | P20591, HS |
Family and domain databases
CDDi | cd08771, DLP_1, 1 hit |
DisProti | DP01239 |
InterProi | View protein in InterPro IPR000375, Dynamin_central IPR001401, Dynamin_GTPase IPR019762, Dynamin_GTPase_CS IPR022812, Dynamin_SF IPR030381, G_DYNAMIN_dom IPR003130, GED IPR020850, GED_dom IPR027417, P-loop_NTPase |
PANTHERi | PTHR11566, PTHR11566, 1 hit |
Pfami | View protein in Pfam PF01031, Dynamin_M, 1 hit PF00350, Dynamin_N, 1 hit PF02212, GED, 1 hit |
PRINTSi | PR00195, DYNAMIN |
SMARTi | View protein in SMART SM00053, DYNc, 1 hit SM00302, GED, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00410, G_DYNAMIN_1, 1 hit PS51718, G_DYNAMIN_2, 1 hit PS51388, GED, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MX1_HUMAN | |
Accessioni | P20591Primary (citable) accession number: P20591 Secondary accession number(s): B2RDA5 Q96CI3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | July 7, 2009 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 21
Human chromosome 21: entries, gene names and cross-references to MIM - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations