UniProtKB - P20459 (IF2A_YEAST)
Eukaryotic translation initiation factor 2 subunit alpha
SUI2
Functioni
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
CuratedMiscellaneous
GO - Molecular functioni
- ribosome binding Source: GO_Central
- translation initiation factor activity Source: GO_Central
GO - Biological processi
- formation of translation preinitiation complex Source: SGD
- translational initiation Source: ComplexPortal
Keywordsi
Molecular function | Initiation factor, RNA-binding |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-381042, PERK regulates gene expression R-SCE-72695, Formation of the ternary complex, and subsequently, the 43S complex R-SCE-72702, Ribosomal scanning and start codon recognition R-SCE-72731, Recycling of eIF2:GDP |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 2 subunit alphaShort name: eIF-2-alpha |
Gene namesi | Ordered Locus Names:YJR007W ORF Names:J1429 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003767, SUI2 |
VEuPathDBi | FungiDB:YJR007W |
Subcellular locationi
Cytosol
- cytosol Source: Reactome
Other locations
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
- eukaryotic 48S preinitiation complex Source: SGD
- eukaryotic translation initiation factor 2 complex Source: SGD
- eukaryotic translation initiation factor 2B complex Source: GO_Central
- multi-eIF complex Source: SGD
- ribosome Source: ComplexPortal
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | S → A: Inhibits derepression of GCN4 expression in amino acid, purine, and glucose-starved cells. 2 Publications | 1 | |
Mutagenesisi | 52 | S → D: Weakly impairs derepression of GCN4 expression in amino acid-starved cells. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000137389 | 1 – 304 | Eukaryotic translation initiation factor 2 subunit alphaAdd BLAST | 304 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | Phosphoserine; by GCN23 Publications | 1 | |
Modified residuei | 292 | PhosphoserineCombined sources | 1 | |
Modified residuei | 294 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | P20459 |
PaxDbi | P20459 |
PRIDEi | P20459 |
PTM databases
iPTMneti | P20459 |
Interactioni
Subunit structurei
Eukaryotic translation initiation factor 2 (eIF-2) is a heterotrimer composed of an alpha, a beta and a gamma subunit. The factors eIF-1, eIF-2, eIF-3, TIF5/eIF-5 and methionyl-tRNAi form a multifactor complex (MFC) that may bind to the 40S ribosome.
CuratedBinary interactionsi
P20459
With | #Exp. | IntAct |
---|---|---|
GCD11 [P32481] | 11 | EBI-8915,EBI-8924 |
TIF5 [P38431] | 4 | EBI-8915,EBI-9038 |
Protein-protein interaction databases
BioGRIDi | 33763, 320 interactors |
ComplexPortali | CPX-427, Eukaryotic translation initiation factor 2 complex |
DIPi | DIP-2327N |
IntActi | P20459, 35 interactors |
MINTi | P20459 |
STRINGi | 4932.YJR007W |
Miscellaneous databases
RNActi | P20459, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P20459 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20459 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 88 | S1 motifPROSITE-ProRule annotationAdd BLAST | 72 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 283 – 304 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2916, Eukaryota |
GeneTreei | ENSGT00390000007015 |
HOGENOMi | CLU_033458_0_1_1 |
InParanoidi | P20459 |
OMAi | DVNEHQR |
Family and domain databases
CDDi | cd04452, S1_IF2_alpha, 1 hit |
Gene3Di | 1.10.150.190, 1 hit 3.30.70.1130, 1 hit |
IDEALi | IID50184 |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR022967, S1_dom IPR003029, S1_domain IPR044126, S1_IF2_alpha IPR024055, TIF2_asu_C IPR024054, TIF2_asu_middle_sf IPR011488, TIF_2_asu |
PANTHERi | PTHR10602, PTHR10602, 1 hit |
Pfami | View protein in Pfam PF07541, EIF_2_alpha, 1 hit PF00575, S1, 1 hit |
SMARTi | View protein in SMART SM00316, S1, 1 hit |
SUPFAMi | SSF110993, SSF110993, 1 hit SSF116742, SSF116742, 1 hit SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS50126, S1, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSTSHCRFYE NKYPEIDDIV MVNVQQIAEM GAYVKLLEYD NIEGMILLSE
60 70 80 90 100
LSRRRIRSIQ KLIRVGKNDV AVVLRVDKEK GYIDLSKRRV SSEDIIKCEE
110 120 130 140 150
KYQKSKTVHS ILRYCAEKFQ IPLEELYKTI AWPLSRKFGH AYEAFKLSII
160 170 180 190 200
DETVWEGIEP PSKDVLDELK NYISKRLTPQ AVKIRADVEV SCFSYEGIDA
210 220 230 240 250
IKDALKSAED MSTEQMQVKV KLVAAPLYVL TTQALDKQKG IEQLESAIEK
260 270 280 290 300
ITEVITKYGG VCNITMPPKA VTATEDAELQ ALLESKELDN RSDSEDDEDE
SDDE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 258 | Y → H in AAS56202 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25552 Genomic DNA Translation: AAA70332.1 X87611 Genomic DNA Translation: CAA60929.1 Z49507 Genomic DNA Translation: CAA89529.1 AY557876 Genomic DNA Translation: AAS56202.1 BK006943 Genomic DNA Translation: DAA08798.1 |
PIRi | A32108 |
RefSeqi | NP_012540.3, NM_001181664.3 |
Genome annotation databases
EnsemblFungii | YJR007W_mRNA; YJR007W; YJR007W |
GeneIDi | 853463 |
KEGGi | sce:YJR007W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M25552 Genomic DNA Translation: AAA70332.1 X87611 Genomic DNA Translation: CAA60929.1 Z49507 Genomic DNA Translation: CAA89529.1 AY557876 Genomic DNA Translation: AAS56202.1 BK006943 Genomic DNA Translation: DAA08798.1 |
PIRi | A32108 |
RefSeqi | NP_012540.3, NM_001181664.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1Q46 | X-ray | 2.86 | A | 2-176 | [»] | |
2A19 | X-ray | 2.50 | A | 4-176 | [»] | |
2A1A | X-ray | 2.80 | A | 4-176 | [»] | |
3J81 | electron microscopy | 4.00 | j | 1-300 | [»] | |
3JAP | electron microscopy | 4.90 | j | 1-304 | [»] | |
3JAQ | electron microscopy | 6.00 | j | 1-304 | [»] | |
6FYX | electron microscopy | 3.05 | j | 1-304 | [»] | |
6FYY | electron microscopy | 3.05 | j | 1-304 | [»] | |
6GSM | electron microscopy | 5.15 | j | 3-265 | [»] | |
6GSN | electron microscopy | 5.75 | j | 3-265 | [»] | |
6I3M | electron microscopy | 3.93 | K/L | 1-304 | [»] | |
6I7T | electron microscopy | 4.61 | K/L | 1-304 | [»] | |
6JLY | X-ray | 3.50 | L/M | 1-304 | [»] | |
6JLZ | X-ray | 3.35 | L/M | 1-304 | [»] | |
6QG0 | electron microscopy | 4.20 | K/L | 1-304 | [»] | |
6QG1 | electron microscopy | 4.20 | K/L | 1-304 | [»] | |
6QG2 | electron microscopy | 4.60 | K/L | 1-304 | [»] | |
6QG3 | electron microscopy | 9.40 | K/L | 1-304 | [»] | |
6QG5 | electron microscopy | 10.10 | K/L | 1-304 | [»] | |
6QG6 | electron microscopy | 4.65 | K/L | 1-304 | [»] | |
SMRi | P20459 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33763, 320 interactors |
ComplexPortali | CPX-427, Eukaryotic translation initiation factor 2 complex |
DIPi | DIP-2327N |
IntActi | P20459, 35 interactors |
MINTi | P20459 |
STRINGi | 4932.YJR007W |
PTM databases
iPTMneti | P20459 |
Proteomic databases
MaxQBi | P20459 |
PaxDbi | P20459 |
PRIDEi | P20459 |
Genome annotation databases
EnsemblFungii | YJR007W_mRNA; YJR007W; YJR007W |
GeneIDi | 853463 |
KEGGi | sce:YJR007W |
Organism-specific databases
SGDi | S000003767, SUI2 |
VEuPathDBi | FungiDB:YJR007W |
Phylogenomic databases
eggNOGi | KOG2916, Eukaryota |
GeneTreei | ENSGT00390000007015 |
HOGENOMi | CLU_033458_0_1_1 |
InParanoidi | P20459 |
OMAi | DVNEHQR |
Enzyme and pathway databases
Reactomei | R-SCE-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-SCE-381042, PERK regulates gene expression R-SCE-72695, Formation of the ternary complex, and subsequently, the 43S complex R-SCE-72702, Ribosomal scanning and start codon recognition R-SCE-72731, Recycling of eIF2:GDP |
Miscellaneous databases
EvolutionaryTracei | P20459 |
PROi | PR:P20459 |
RNActi | P20459, protein |
Family and domain databases
CDDi | cd04452, S1_IF2_alpha, 1 hit |
Gene3Di | 1.10.150.190, 1 hit 3.30.70.1130, 1 hit |
IDEALi | IID50184 |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR022967, S1_dom IPR003029, S1_domain IPR044126, S1_IF2_alpha IPR024055, TIF2_asu_C IPR024054, TIF2_asu_middle_sf IPR011488, TIF_2_asu |
PANTHERi | PTHR10602, PTHR10602, 1 hit |
Pfami | View protein in Pfam PF07541, EIF_2_alpha, 1 hit PF00575, S1, 1 hit |
SMARTi | View protein in SMART SM00316, S1, 1 hit |
SUPFAMi | SSF110993, SSF110993, 1 hit SSF116742, SSF116742, 1 hit SSF50249, SSF50249, 1 hit |
PROSITEi | View protein in PROSITE PS50126, S1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IF2A_YEAST | |
Accessioni | P20459Primary (citable) accession number: P20459 Secondary accession number(s): D6VWI2, E9P8T6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 198 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - Translation initiation factors
List of translation initiation factor entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families