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UniProtKB - P20447 (DBP3_YEAST)
Protein
ATP-dependent RNA helicase DBP3
Gene
DBP3
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.
1 PublicationMiscellaneous
Present with 38900 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 156 – 163 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP-dependent activity, acting on RNA Source: SGD
- ATP hydrolysis activity Source: RHEA
- RNA binding Source: GO_Central
- RNA helicase activity Source: SGD
GO - Biological processi
- endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
- rRNA processing Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Ribosome biogenesis, rRNA processing |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: ATP-dependent RNA helicase DBP3 (EC:3.6.4.13)Alternative name(s): DEAD box protein 3 Helicase CA3 |
Gene namesi | Name:DBP3 Ordered Locus Names:YGL078C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003046, DBP3 |
VEuPathDBi | FungiDB:YGL078C |
Subcellular locationi
Nucleus
- nucleolus 2 Publications
Nucleus
- nucleolus Source: SGD
Other locations
- preribosome, large subunit precursor Source: SGD
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055017 | 1 – 523 | ATP-dependent RNA helicase DBP3Add BLAST | 523 |
Proteomic databases
MaxQBi | P20447 |
PaxDbi | P20447 |
PRIDEi | P20447 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 33172, 272 interactors |
DIPi | DIP-2668N |
IntActi | P20447, 13 interactors |
MINTi | P20447 |
STRINGi | 4932.YGL078C |
Miscellaneous databases
RNActi | P20447, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 143 – 315 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 173 | |
Domaini | 344 – 493 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 150 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 71 | DisorderedSequence analysisAdd BLAST | 71 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 114 – 140 | Q motifAdd BLAST | 27 | |
Motifi | 262 – 265 | DEAD box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 23 | Basic and acidic residuesSequence analysisAdd BLAST | 23 | |
Compositional biasi | 24 – 48 | Basic residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 49 – 68 | Basic and acidic residuesSequence analysisAdd BLAST | 20 |
Domaini
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0331, Eukaryota |
HOGENOMi | CLU_003041_1_5_1 |
InParanoidi | P20447 |
OMAi | KCVCLYG |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
i Sequence
Sequence statusi: Complete.
P20447-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKEEIADKK RKVVDEEVIE KKKSKKHKKD KKDKKEKKDK KHKKHKKEKK
60 70 80 90 100
GEKEVEVPEK ESEKKPEPTS AVASEFYVQS EALTSLPQSD IDEYFKENEI
110 120 130 140 150
AVEDSLDLAL RPLLSFDYLS LDSSIQAEIS KFPKPTPIQA VAWPYLLSGK
160 170 180 190 200
DVVGVAETGS GKTFAFGVPA ISHLMNDQKK RGIQVLVISP TRELASQIYD
210 220 230 240 250
NLIVLTDKVG MQCCCVYGGV PKDEQRIQLK KSQVVVATPG RLLDLLQEGS
260 270 280 290 300
VDLSQVNYLV LDEADRMLEK GFEEDIKNII RETDASKRQT LMFTATWPKE
310 320 330 340 350
VRELASTFMN NPIKVSIGNT DQLTANKRIT QIVEVVDPRG KERKLLELLK
360 370 380 390 400
KYHSGPKKNE KVLIFALYKK EAARVERNLK YNGYNVAAIH GDLSQQQRTQ
410 420 430 440 450
ALNEFKSGKS NLLLATDVAA RGLDIPNVKT VINLTFPLTV EDYVHRIGRT
460 470 480 490 500
GRAGQTGTAH TLFTEQEKHL AGGLVNVLNG ANQPVPEDLI KFGTHTKKKE
510 520
HSAYGSFFKD VDLTKKPKKI TFD
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 441 | E → A no nucleotide entry (PubMed:2406722).Curated | 1 | |
Sequence conflicti | 444 | V → I no nucleotide entry (PubMed:2406722).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80437 Genomic DNA Translation: AAA73137.1 Z72600 Genomic DNA Translation: CAA96783.1 BK006941 Genomic DNA Translation: DAA08027.1 |
PIRi | S30805 |
RefSeqi | NP_011437.3, NM_001180943.3 |
Genome annotation databases
EnsemblFungii | YGL078C_mRNA; YGL078C; YGL078C |
GeneIDi | 852802 |
KEGGi | sce:YGL078C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M80437 Genomic DNA Translation: AAA73137.1 Z72600 Genomic DNA Translation: CAA96783.1 BK006941 Genomic DNA Translation: DAA08027.1 |
PIRi | S30805 |
RefSeqi | NP_011437.3, NM_001180943.3 |
3D structure databases
SMRi | P20447 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33172, 272 interactors |
DIPi | DIP-2668N |
IntActi | P20447, 13 interactors |
MINTi | P20447 |
STRINGi | 4932.YGL078C |
Proteomic databases
MaxQBi | P20447 |
PaxDbi | P20447 |
PRIDEi | P20447 |
Genome annotation databases
EnsemblFungii | YGL078C_mRNA; YGL078C; YGL078C |
GeneIDi | 852802 |
KEGGi | sce:YGL078C |
Organism-specific databases
SGDi | S000003046, DBP3 |
VEuPathDBi | FungiDB:YGL078C |
Phylogenomic databases
eggNOGi | KOG0331, Eukaryota |
HOGENOMi | CLU_003041_1_5_1 |
InParanoidi | P20447 |
OMAi | KCVCLYG |
Miscellaneous databases
PROi | PR:P20447 |
RNActi | P20447, protein |
Family and domain databases
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DBP3_YEAST | |
Accessioni | P20447Primary (citable) accession number: P20447 Secondary accession number(s): D6VU66 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names - SIMILARITY comments
Index of protein domains and families