DBP3

Functionⁱ

ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.1 Publication

Miscellaneous

Present with 38900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

ATP + H₂O = ADP + phosphate.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	156 – 163	ATPPROSITE-ProRule annotation		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ATP-dependent RNA helicase activity
Source: SGD
Inferred from direct assayⁱ
- 23153376
RNA binding Source: UniProtKB-KW
RNA-dependent ATPase activity
Source: SGD
Inferred from direct assayⁱ
- 18975973
- 23153376

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Helicase, Hydrolase, RNA-binding
Biological process	Ribosome biogenesis, rRNA processing
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30579-MONOMER

BioCycⁱ

YEAST:G3O-30579-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATP-dependent RNA helicase DBP3 (EC:3.6.4.13) Alternative name(s): DEAD box protein 3 Helicase CA3
Gene namesⁱ	Name:DBP3 Ordered Locus Names:YGL078C
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome VII

Organism-specific databases

EuPathDBⁱ	FungiDB:YGL078C
Saccharomyces Genome Database More...SGDⁱ	S000003046 DBP3

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000055017	1 – 523	ATP-dependent RNA helicase DBP3Add BLAST		523

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P20447
PaxDbⁱ	P20447
PRIDEⁱ	P20447

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	33172, 236 interactors
Database of interacting proteins More...DIPⁱ	DIP-2668N
IntActⁱ	P20447, 13 interactors
Molecular INTeraction database More...MINTⁱ	P20447
STRINGⁱ	4932.YGL078C

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P20447
SMRⁱ	P20447
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	143 – 315	Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST		173
Domainⁱ	344 – 493	Helicase C-terminalPROSITE-ProRule annotationAdd BLAST		150

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	114 – 140	Q motifAdd BLAST		27
Motifⁱ	262 – 265	DEAD box		4

Domainⁱ

The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.

Sequence similaritiesⁱ

Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00920000149066
HOGENOMⁱ	HOG000268804
InParanoidⁱ	P20447
KEGG Orthology (KO) More...KOⁱ	K14811
OMAⁱ	AYGAFFK
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C1B4A

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00079 HELICc, 1 hit
InterProⁱ	View protein in InterPro IPR011545 DEAD/DEAH_box_helicase_dom IPR014001 Helicase_ATP-bd IPR001650 Helicase_C IPR027417 P-loop_NTPase IPR000629 RNA-helicase_DEAD-box_CS IPR014014 RNA_helicase_DEAD_Q_motif
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00270 DEAD, 1 hit PF00271 Helicase_C, 1 hit
SMARTⁱ	View protein in SMART SM00487 DEXDc, 1 hit SM00490 HELICc, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00039 DEAD_ATP_HELICASE, 1 hit PS51192 HELICASE_ATP_BIND_1, 1 hit PS51194 HELICASE_CTER, 1 hit PS51195 Q_MOTIF, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P20447-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTKEEIADKK RKVVDEEVIE KKKSKKHKKD KKDKKEKKDK KHKKHKKEKK 
        60         70         80         90        100
GEKEVEVPEK ESEKKPEPTS AVASEFYVQS EALTSLPQSD IDEYFKENEI 
       110        120        130        140        150
AVEDSLDLAL RPLLSFDYLS LDSSIQAEIS KFPKPTPIQA VAWPYLLSGK 
       160        170        180        190        200
DVVGVAETGS GKTFAFGVPA ISHLMNDQKK RGIQVLVISP TRELASQIYD 
       210        220        230        240        250
NLIVLTDKVG MQCCCVYGGV PKDEQRIQLK KSQVVVATPG RLLDLLQEGS 
       260        270        280        290        300
VDLSQVNYLV LDEADRMLEK GFEEDIKNII RETDASKRQT LMFTATWPKE 
       310        320        330        340        350
VRELASTFMN NPIKVSIGNT DQLTANKRIT QIVEVVDPRG KERKLLELLK 
       360        370        380        390        400
KYHSGPKKNE KVLIFALYKK EAARVERNLK YNGYNVAAIH GDLSQQQRTQ 
       410        420        430        440        450
ALNEFKSGKS NLLLATDVAA RGLDIPNVKT VINLTFPLTV EDYVHRIGRT 
       460        470        480        490        500
GRAGQTGTAH TLFTEQEKHL AGGLVNVLNG ANQPVPEDLI KFGTHTKKKE 
       510        520 
HSAYGSFFKD VDLTKKPKKI TFD

Length:523

Mass (Da):58,827

Last modified:October 1, 1996 - v2

Checksum:ⁱC198CF4E8D5BAFAA

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	441	E → A no nucleotide entry (PubMed:2406722).Curated		1
Sequence conflictⁱ	444	V → I no nucleotide entry (PubMed:2406722).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M80437 Genomic DNA Translation: AAA73137.1 Z72600 Genomic DNA Translation: CAA96783.1 BK006941 Genomic DNA Translation: DAA08027.1
PIRⁱ	S30805
NCBI Reference Sequences More...RefSeqⁱ	NP_011437.3, NM_001180943.3

Genome annotation databases

EnsemblFungiⁱ	YGL078C; YGL078C; YGL078C
GeneIDⁱ	852802
KEGGⁱ	sce:YGL078C

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P20447	Dbp3p		YEASC		523	UniRef100_P20447
Dbp3p		YEASB		349
Dbp3p		YEASV		349

Protein	Similar proteins		Species	Score	Length	Source
P20447	ATP-dependent RNA helicase DBP3		YEAS7		523	UniRef90_P20447
Dbp3p		YEASC		523
Dbp3p		YEASB		349
Dbp3p		YEASV		349
Dbp3p		YEASZ		537
+12

Protein	Similar proteins		Species	Score	Length	Source
P20447	ATP-dependent RNA helicase DBP3		YEAS7		523	UniRef50_P20447
ATP-dependent RNA helicase DBP3		LODEL		535
ATP-dependent RNA helicase DBP3		COCIM		515
ATP-dependent RNA helicase DBP3		CANAL		564
ATP-dependent RNA helicase DBP3		ASHGO		535
+114

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M80437 Genomic DNA Translation: AAA73137.1 Z72600 Genomic DNA Translation: CAA96783.1 BK006941 Genomic DNA Translation: DAA08027.1
PIRⁱ	S30805
RefSeqⁱ	NP_011437.3, NM_001180943.3

3D structure databases

ProteinModelPortalⁱ	P20447
SMRⁱ	P20447
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	33172, 236 interactors
DIPⁱ	DIP-2668N
IntActⁱ	P20447, 13 interactors
MINTⁱ	P20447
STRINGⁱ	4932.YGL078C

Proteomic databases

MaxQBⁱ	P20447
PaxDbⁱ	P20447
PRIDEⁱ	P20447

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YGL078C; YGL078C; YGL078C
GeneIDⁱ	852802
KEGGⁱ	sce:YGL078C

Organism-specific databases

EuPathDBⁱ	FungiDB:YGL078C
SGDⁱ	S000003046 DBP3

Phylogenomic databases

GeneTreeⁱ	ENSGT00920000149066
HOGENOMⁱ	HOG000268804
InParanoidⁱ	P20447
KOⁱ	K14811
OMAⁱ	AYGAFFK
OrthoDBⁱ	EOG092C1B4A

Enzyme and pathway databases

BioCycⁱ	YEAST:G3O-30579-MONOMER

BioCycⁱ

YEAST:G3O-30579-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P20447

PROⁱ

PR:P20447

Family and domain databases

CDDⁱ	cd00079 HELICc, 1 hit
InterProⁱ	View protein in InterPro IPR011545 DEAD/DEAH_box_helicase_dom IPR014001 Helicase_ATP-bd IPR001650 Helicase_C IPR027417 P-loop_NTPase IPR000629 RNA-helicase_DEAD-box_CS IPR014014 RNA_helicase_DEAD_Q_motif
Pfamⁱ	View protein in Pfam PF00270 DEAD, 1 hit PF00271 Helicase_C, 1 hit
SMARTⁱ	View protein in SMART SM00487 DEXDc, 1 hit SM00490 HELICc, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit
PROSITEⁱ	View protein in PROSITE PS00039 DEAD_ATP_HELICASE, 1 hit PS51192 HELICASE_ATP_BIND_1, 1 hit PS51194 HELICASE_CTER, 1 hit PS51195 Q_MOTIF, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DBP3_YEAST
Accessionⁱ	P20447Primary (citable) accession number: P20447 Secondary accession number(s): D6VU66
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	October 1, 1996
	Last modified:	September 12, 2018
	This is version 174 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome VII
Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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UniParc

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UniProtKB - P20447 (DBP3_YEAST)

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ATP-dependent RNA helicase DBP3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ