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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC3

Gene

RPB8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription. of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.3 Publications

Miscellaneous

Present with 6210 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • RNA polymerase I activity Source: UniProtKB

GO - Biological processi

  • ribosome biogenesis Source: UniProtKB-KW
  • termination of RNA polymerase III transcription Source: Reactome
  • transcription, RNA-templated Source: GOC
  • transcription by RNA polymerase I Source: UniProtKB
  • transcription by RNA polymerase II Source: SGD
  • transcription by RNA polymerase III Source: SGD
  • tRNA transcription by RNA polymerase III Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processRibosome biogenesis, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-33723-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-72086 mRNA Capping
R-SCE-72165 mRNA Splicing - Minor Pathway
R-SCE-73762 RNA Polymerase I Transcription Initiation
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Short name:
RNA polymerases I, II, and III subunit ABC3
Alternative name(s):
ABC14.4
ABC14.5
DNA-directed RNA polymerases I, II, and III 14.5 kDa polypeptide
Gene namesi
Name:RPB8
Ordered Locus Names:YOR224C
ORF Names:YOR50-14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR224C
SGDiS000005750 RPB8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000740012 – 146DNA-directed RNA polymerases I, II, and III subunit RPABC3Add BLAST145

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei68PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP20436
PaxDbiP20436
PRIDEiP20436

PTM databases

iPTMnetiP20436

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits: RPO21, RPB2, RPB3, RPB4, RPB5, RPO26, RPB7, RPB8, RPB9, RPB10 and RPC10. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Directly interacts with POLR2A.7 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34618, 213 interactors
ComplexPortaliCPX-1664 DNA-directed RNA Polymerase I complex
CPX-2660 DNA-directed RNA polymerase III complex
CPX-2662 DNA-directed RNA polymerase II complex
DIPiDIP-2195N
IntActiP20436, 37 interactors
MINTiP20436
STRINGi4932.YOR224C

Structurei

Secondary structure

1146
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 16Combined sources10
Beta strandi21 – 32Combined sources12
Beta strandi37 – 43Combined sources7
Helixi44 – 46Combined sources3
Beta strandi54 – 58Combined sources5
Turni84 – 86Combined sources3
Helixi89 – 92Combined sources4
Beta strandi93 – 101Combined sources9
Beta strandi108 – 110Combined sources3
Beta strandi112 – 118Combined sources7
Beta strandi121 – 128Combined sources8
Turni129 – 133Combined sources5
Beta strandi136 – 138Combined sources3
Beta strandi140 – 145Combined sources6

3D structure databases

ProteinModelPortaliP20436
SMRiP20436
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20436

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni16 – 39Non-specific ssDNA bindingBy similarityAdd BLAST24

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000018195
HOGENOMiHOG000175572
InParanoidiP20436
KOiK03016
OMAiFEYVMFG
OrthoDBiEOG092C57ZC

Family and domain databases

InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR005570 RNA_pol_Rpb8
PANTHERiPTHR10917 PTHR10917, 1 hit
PfamiView protein in Pfam
PF03870 RNA_pol_Rpb8, 1 hit
PIRSFiPIRSF000779 RNA_pol_Rpb8, 1 hit
SMARTiView protein in SMART
SM00658 RPOL8c, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNTLFDDIF QVSEVDPGRY NKVCRIEAAS TTQDQCKLTL DINVELFPVA
60 70 80 90 100
AQDSLTVTIA SSLNLEDTPA NDSSATRSWR PPQAGDRSLA DDYDYVMYGT
110 120 130 140
AYKFEEVSKD LIAVYYSFGG LLMRLEGNYR NLNNLKQENA YLLIRR
Length:146
Mass (Da):16,511
Last modified:February 1, 1991 - v1
Checksum:i5B71693FE6FD8863
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53289 Genomic DNA Translation: CAA37383.1
X92441 Genomic DNA Translation: CAA63187.1
Z75132 Genomic DNA Translation: CAA99443.1
AY693087 Genomic DNA Translation: AAT93106.1
BK006948 Genomic DNA Translation: DAA10995.1
PIRiC34588
RefSeqiNP_014867.1, NM_001183643.1

Genome annotation databases

EnsemblFungiiYOR224C; YOR224C; YOR224C
GeneIDi854399
KEGGisce:YOR224C

Similar proteinsi

Entry informationi

Entry nameiRPAB3_YEAST
AccessioniPrimary (citable) accession number: P20436
Secondary accession number(s): D6W2S9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 18, 2018
This is version 187 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

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