Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC2

Gene

RPO26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. RNA polymerases are composed of mobile elements that move relative to each other. In Pol II, RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.3 Publications

Miscellaneous

Present with 6090 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • DNA binding Source: InterPro
  • RNA polymerase I activity Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processRibosome biogenesis, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-34310-MONOMER
ReactomeiR-SCE-113418 Formation of the Early Elongation Complex
R-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-6781823 Formation of TC-NER Pre-Incision Complex
R-SCE-6782135 Dual incision in TC-NER
R-SCE-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-SCE-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-SCE-6807505 RNA polymerase II transcribes snRNA genes
R-SCE-72086 mRNA Capping
R-SCE-72165 mRNA Splicing - Minor Pathway
R-SCE-73762 RNA Polymerase I Transcription Initiation
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-SCE-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-SCE-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Short name:
RNA polymerases I, II, and III subunit ABC2
Alternative name(s):
ABC23
DNA-directed RNA polymerases I, II, and III 23 kDa polypeptide
Gene namesi
Name:RPO26
Synonyms:RPB6
Ordered Locus Names:YPR187W
ORF Names:P9677.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR187W
SGDiS000006391 RPO26

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001337971 – 155DNA-directed RNA polymerases I, II, and III subunit RPABC2Add BLAST155

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP20435
PaxDbiP20435
PRIDEiP20435

PTM databases

iPTMnetiP20435

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits: RPO21, RPB2, RPB3, RPB4, RPB5, RPO26, RPB7, RPB8, RPB9, RPB10 and RPC10. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.9 Publications

Protein-protein interaction databases

BioGridi36359, 101 interactors
ComplexPortaliCPX-1664 DNA-directed RNA Polymerase I complex
CPX-2660 DNA-directed RNA polymerase III complex
CPX-2662 DNA-directed RNA polymerase II complex
DIPiDIP-2194N
IntActiP20435, 41 interactors
MINTiP20435
STRINGi4932.YPR187W

Structurei

Secondary structure

1155
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00771
ProteinModelPortaliP20435
SMRiP20435
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20435

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni111 – 132Leucine-zipperAdd BLAST22

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000010415
HOGENOMiHOG000225272
InParanoidiP20435
KOiK03014
OMAiRTIVNGG
OrthoDBiEOG092C5A9A

Family and domain databases

Gene3Di3.90.940.10, 1 hit
HAMAPiMF_00192 RNApol_arch_K, 1 hit
InterProiView protein in InterPro
IPR020708 DNA-dir_RNA_polK_14-18kDa_CS
IPR006110 Pol_omega/K/RPB6
IPR012293 RNAP_RPB6_omega
IPR028363 RPB6
IPR036161 RPB6/omega_like_sf
IPR006111 RpoK/Rpb6
PfamiView protein in Pfam
PF01192 RNA_pol_Rpb6, 1 hit
PIRSFiPIRSF500154 RPB6, 1 hit
PIRSF000778 RpoK/RPB6, 1 hit
SMARTiView protein in SMART
SM01409 RNA_pol_Rpb6, 1 hit
SUPFAMiSSF63562 SSF63562, 1 hit
PROSITEiView protein in PROSITE
PS01111 RNA_POL_K_14KD, 1 hit

Sequencei

Sequence statusi: Complete.

P20435-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDYEEAFND GNENFEDFDV EHFSDEETYE EKPQFKDGET TDANGKTIVT
60 70 80 90 100
GGNGPEDFQQ HEQIRRKTLK EKAIPKDQRA TTPYMTKYER ARILGTRALQ
110 120 130 140 150
ISMNAPVFVD LEGETDPLRI AMKELAEKKI PLVIRRYLPD GSFEDWSVEE

LIVDL
Length:155
Mass (Da):17,910
Last modified:February 1, 1991 - v1
Checksum:iAA3DEF529F94A5E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53288 Genomic DNA Translation: CAA37382.1
M33924 Genomic DNA Translation: AAA34989.1
U25841 Genomic DNA Translation: AAB64616.1
BK006949 Genomic DNA Translation: DAA11603.1
PIRiS13307 RNBYR6
RefSeqiNP_015513.1, NM_001184284.1

Genome annotation databases

EnsemblFungiiYPR187W; YPR187W; YPR187W
GeneIDi856317
KEGGisce:YPR187W

Similar proteinsi

Entry informationi

Entry nameiRPAB2_YEAST
AccessioniPrimary (citable) accession number: P20435
Secondary accession number(s): D6W4I7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 12, 2018
This is version 195 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again