AHA3

Functionⁱ

The plasma membrane H⁺ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H⁺-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityⁱ

ATP + H₂O + H⁺(In) = ADP + phosphate + H⁺(Out).

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	330	4-aspartylphosphate intermediateBy similarity	1
Metal bindingⁱ	589	MagnesiumBy similarity	1
Metal bindingⁱ	593	MagnesiumBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
magnesium ion binding
Source: UniProtKB
Inferred from direct assayⁱ
- 8300635
proton-exporting ATPase activity, phosphorylative mechanism
Source: UniProtKB
Inferred from direct assayⁱ
- 8300635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

ATP hydrolysis coupled proton transport
Source: UniProtKB
Inferred from direct assayⁱ
- 8300635
proton export across plasma membrane Source: InterPro
proton transmembrane transport
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
regulation of intracellular pH
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Translocase
Biological process	Hydrogen ion transport, Ion transport, Transport
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	ARA:AT5G57350-MONOMER

BioCycⁱ

ARA:AT5G57350-MONOMER

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: ATPase 3, plasma membrane-type (EC:7.1.2.1) Alternative name(s): Proton pump 3
Gene namesⁱ	Name:AHA3 Ordered Locus Names:At5g57350 ORF Names:MJB24.16
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 5

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT5G57350
TAIRⁱ	locus:2165600 AT5G57350

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 62	CytoplasmicSequence analysisAdd BLAST	62
Transmembraneⁱ	63 – 82	Helical; Name=1Sequence analysisAdd BLAST	20
Topological domainⁱ	83 – 94	ExtracellularSequence analysisAdd BLAST	12
Transmembraneⁱ	95 – 115	Helical; Name=2Sequence analysisAdd BLAST	21
Topological domainⁱ	116 – 244	CytoplasmicSequence analysisAdd BLAST	129
Transmembraneⁱ	245 – 265	Helical; Name=3Sequence analysisAdd BLAST	21
Topological domainⁱ	266 – 274	ExtracellularSequence analysis	9
Transmembraneⁱ	275 – 292	Helical; Name=4Sequence analysisAdd BLAST	18
Topological domainⁱ	293 – 644	CytoplasmicSequence analysisAdd BLAST	352
Transmembraneⁱ	645 – 666	Helical; Name=5Sequence analysisAdd BLAST	22
Topological domainⁱ	667 – 671	ExtracellularSequence analysis	5
Transmembraneⁱ	672 – 694	Helical; Name=6Sequence analysisAdd BLAST	23
Topological domainⁱ	695 – 710	CytoplasmicSequence analysisAdd BLAST	16
Transmembraneⁱ	711 – 731	Helical; Name=7Sequence analysisAdd BLAST	21
Topological domainⁱ	732 – 752	ExtracellularSequence analysisAdd BLAST	21
Transmembraneⁱ	753 – 773	Helical; Name=8Sequence analysisAdd BLAST	21
Topological domainⁱ	774 – 785	CytoplasmicSequence analysisAdd BLAST	12
Transmembraneⁱ	786 – 806	Helical; Name=9Sequence analysisAdd BLAST	21
Topological domainⁱ	807 – 814	ExtracellularSequence analysis	8
Transmembraneⁱ	815 – 835	Helical; Name=10Sequence analysisAdd BLAST	21
Topological domainⁱ	836 – 949	CytoplasmicSequence analysisAdd BLAST	114

Keywords - Cellular componentⁱ

Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	947	Y → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication	1
Mutagenesisⁱ	948	T → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication	1
Mutagenesisⁱ	949	V → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000046276	1 – 949	ATPase 3, plasma membrane-typeAdd BLAST		949

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	882	PhosphothreonineCombined sources	1
Modified residueⁱ	932	PhosphoserineBy similarity	1
Modified residueⁱ	948	Phosphothreonine1 Publication	1

Post-translational modificationⁱ

Phosphorylation level varies significantly during early response to bacterial elicitor (e.g. fusicoccin FC).1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P20431
PRIDEⁱ	P20431

PTM databases

iPTMnetⁱ	P20431

iPTMnetⁱ

P20431

Expressionⁱ

Tissue specificityⁱ

Found predominantly in phloem cells of leaves, stems, roots and flowers.

Gene expression databases

ExpressionAtlasⁱ	P20431 baseline and differential
Genevisibleⁱ	P20431 AT

Interactionⁱ

Subunit structureⁱ

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H⁺-ATPase.

Protein-protein interaction databases

BioGridⁱ	21085, 4 interactors
STRINGⁱ	3702.AT5G57350.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P20431
SMRⁱ	P20431
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	947 – 949	Interaction with 14-3-3 proteins		3

Sequence similaritiesⁱ

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0205 Eukaryota COG0474 LUCA
HOGENOMⁱ	HOG000160005
InParanoidⁱ	P20431
KEGG Orthology (KO) More...KOⁱ	K01535
OMAⁱ	HHISAYF
Database of Orthologous Groups More...OrthoDBⁱ	EOG093602AD
PhylomeDBⁱ	P20431

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02076 P-type_ATPase_H, 1 hit
Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006534 P-type_ATPase_IIIA IPR001757 P_typ_ATPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00690 Cation_ATPase_N, 1 hit
PRINTSⁱ	PR00120 HATPASE
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81665 SSF81665, 1 hit
TIGRFAMsⁱ	TIGR01647 ATPase-IIIA_H, 1 hit TIGR01494 ATPase_P-type, 2 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P20431-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MASGLEDIVN ENVDLEKIPI EEVFQQLKCS REGLSGAEGE NRLQIFGPNK 
        60         70         80         90        100
LEEKKESKLL KFLGFMWNPL SWVMEAAAIM AIALANGGGK PPDWQDFVGI 
       110        120        130        140        150
VCLLVINSTI SFVEENNAGN AAAALMAGLA PKTKVLRDGK WSEQEASILV 
       160        170        180        190        200
PGDIVSIKLG DIIPADARLL EGDPLKVDQS ALTGESLPAT KGPGEEVFSG 
       210        220        230        240        250
STCKQGEIEA VVIATGVHTF FGKAAHLVDS TNQVGHFQKV LTAIGNFCIC 
       260        270        280        290        300
SIAVGIAIEI VVMYPIQRRH YRDGIDNLLV LLIGGIPIAM PTVLSVTMAI 
       310        320        330        340        350
GSHKLSQQGA ITKRMTAIEE MAGMDVLCSD KTGTLTLNKL SVDKNLIEVY 
       360        370        380        390        400
CKGVEKDEVL LFAARASRVE NQDAIDAAMV GMLADPKEAR AGIREIHFLP 
       410        420        430        440        450
FNPVDKRTAL TFIDSNGNWH RVSKGAPEQI LDLCNARADL RKRVHSTIDK 
       460        470        480        490        500
YAERGLRSLA VSRQTVPEKT KESSGSPWEF VGVLPLFDPP RHDSAETIRR 
       510        520        530        540        550
ALDLGVNVKM ITGDQLAIAK ETGRRLGMGS NMYPSSSLLG KHKDEAMAHI 
       560        570        580        590        600
PVEDLIEKAD GFAGVFPEHK YEIVKKLQER KHICGMTGDG VNDAPALKKA 
       610        620        630        640        650
DIGIAVADAT DAARGASDIV LTEPGLSVII SAVLTSRAIF QRMKNYTIYA 
       660        670        680        690        700
VSITIRIVFG FMLIALIWKF DFSPFMVLII AILNDGTIMT ISKDRVKPSP 
       710        720        730        740        750
TPDSWKLKEI FATGVVLGGY MAIMTVVFFW AAYKTDFFPR TFHVRDLRGS 
       760        770        780        790        800
EHEMMSALYL QVSIVSQALI FVTRSRSWSF TERPGYFLLI AFWVAQLIAT 
       810        820        830        840        850
AIAVYGNWEF ARIKGIGWGW AGVIWLYSIV FYFPLDIMKF AIRYILAGTA 
       860        870        880        890        900
WKNIIDNRTA FTTKQNYGIE EREAQWAHAQ RTLHGLQNTE TANVVPERGG 
       910        920        930        940 
YRELSEIANQ AKRRAEIARL RELHTLKGHV ESVVKLKGLD IETAGHYTV

Length:949

Mass (Da):104,450

Last modified:January 23, 2007 - v2

Checksum:ⁱ3F88C87D849A4001

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1P8BD43	A0A1P8BD43_ARATH		Plasma membrane ATPase Plasma membrane ATPase, EC 3.6.3.6	HA3 AHA3, ATAHA3, H(+)-ATPase 3, MJB24.16, MJB24_16	687	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J04737 Genomic DNA Translation: AAA32750.1 AB019233, AB016891 Genomic DNA Translation: BAB09963.1 CP002688 Genomic DNA Translation: AED96889.1 CP002688 Genomic DNA Translation: AED96890.1 CP002688 Genomic DNA Translation: ANM69534.1 AY072153 mRNA Translation: AAL59975.1 X60115 Genomic DNA Translation: CAA42714.1
PIRⁱ	A33698 PXMUP3
NCBI Reference Sequences More...RefSeqⁱ	NP_001190559.1, NM_001203630.1 NP_001331204.1, NM_001345259.1 NP_200545.1, NM_125118.3
UniGeneⁱ	At.290

Genome annotation databases

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P20431	Plasma membrane ATPase		ARATH		949	UniRef100_P20431
Plasma membrane ATPase		ARATH		687
Plasma membrane ATPase 3 (Fragment)		ARATH		397
ATPase 3 (Fragment)		ARATH		232

Protein	Similar proteins		Species	Score	Length	Source
P20431	Plasma membrane ATPase		ARATH		949	UniRef90_P20431
Plasma membrane ATPase		BRARP		952
Plasma membrane ATPase		Brassica oleracea var. oleracea		952
Plasma membrane ATPase		BRANA		952
Plasma membrane ATPase		ARAAL		951
+22

Protein	Similar proteins		Species	Score	Length	Source
P20431	ATPase 2, plasma membrane-type	)	ARATH		948	UniRef50_P19456
Plasma membrane ATPase 4		NICPL		952
Plasma membrane ATPase		EUTHA		948
Plasma membrane ATPase		ARAAL		948
ATPase 2, plasma membrane-type (Fragment)		NOCCA		334
+1418

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J04737 Genomic DNA Translation: AAA32750.1 AB019233, AB016891 Genomic DNA Translation: BAB09963.1 CP002688 Genomic DNA Translation: AED96889.1 CP002688 Genomic DNA Translation: AED96890.1 CP002688 Genomic DNA Translation: ANM69534.1 AY072153 mRNA Translation: AAL59975.1 X60115 Genomic DNA Translation: CAA42714.1
PIRⁱ	A33698 PXMUP3
RefSeqⁱ	NP_001190559.1, NM_001203630.1 NP_001331204.1, NM_001345259.1 NP_200545.1, NM_125118.3
UniGeneⁱ	At.290

3D structure databases

ProteinModelPortalⁱ	P20431
SMRⁱ	P20431
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	21085, 4 interactors
STRINGⁱ	3702.AT5G57350.1

PTM databases

iPTMnetⁱ	P20431

iPTMnetⁱ

P20431

Proteomic databases

PaxDbⁱ	P20431
PRIDEⁱ	P20431

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT5G57350.1; AT5G57350.1; AT5G57350 AT5G57350.2; AT5G57350.2; AT5G57350 AT5G57350.4; AT5G57350.4; AT5G57350
GeneIDⁱ	835841
Grameneⁱ	AT5G57350.1; AT5G57350.1; AT5G57350 AT5G57350.2; AT5G57350.2; AT5G57350 AT5G57350.4; AT5G57350.4; AT5G57350
KEGGⁱ	ath:AT5G57350

Organism-specific databases

Araportⁱ	AT5G57350
TAIRⁱ	locus:2165600 AT5G57350

Phylogenomic databases

eggNOGⁱ	KOG0205 Eukaryota COG0474 LUCA
HOGENOMⁱ	HOG000160005
InParanoidⁱ	P20431
KOⁱ	K01535
OMAⁱ	HHISAYF
OrthoDBⁱ	EOG093602AD
PhylomeDBⁱ	P20431

Enzyme and pathway databases

BioCycⁱ	ARA:AT5G57350-MONOMER

BioCycⁱ

ARA:AT5G57350-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P20431

PROⁱ

PR:P20431

Gene expression databases

ExpressionAtlasⁱ	P20431 baseline and differential
Genevisibleⁱ	P20431 AT

Family and domain databases

CDDⁱ	cd02076 P-type_ATPase_H, 1 hit
Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 1 hit
InterProⁱ	View protein in InterPro IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006534 P-type_ATPase_IIIA IPR001757 P_typ_ATPase
Pfamⁱ	View protein in Pfam PF00690 Cation_ATPase_N, 1 hit
PRINTSⁱ	PR00120 HATPASE
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 1 hit SSF81653 SSF81653, 1 hit SSF81665 SSF81665, 1 hit
TIGRFAMsⁱ	TIGR01647 ATPase-IIIA_H, 1 hit TIGR01494 ATPase_P-type, 2 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PMA3_ARATH
Accessionⁱ	P20431Primary (citable) accession number: P20431
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - P20431 (PMA3_ARATH)

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ATPase 3, plasma membrane-type

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ