UniProtKB - P20431 (PMA3_ARATH)
Protein
ATPase 3, plasma membrane-type
Gene
AHA3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Catalytic activityi
- EC:7.1.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 330 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 589 | MagnesiumBy similarity | 1 | |
Metal bindingi | 593 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: UniProtKB
- proton-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB
GO - Biological processi
- proton export across plasma membrane Source: InterPro
- proton transmembrane transport Source: UniProtKB
- regulation of intracellular pH Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT5G57350-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:AHA3 Ordered Locus Names:At5g57350 ORF Names:MJB24.16 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G57350 |
TAIRi | locus:2165600, AT5G57350 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB
- plasmodesma Source: TAIR
- plastid Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 62 | CytoplasmicSequence analysisAdd BLAST | 62 | |
Transmembranei | 63 – 82 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Topological domaini | 83 – 94 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 95 – 115 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 116 – 244 | CytoplasmicSequence analysisAdd BLAST | 129 | |
Transmembranei | 245 – 265 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 266 – 274 | ExtracellularSequence analysis | 9 | |
Transmembranei | 275 – 292 | Helical; Name=4Sequence analysisAdd BLAST | 18 | |
Topological domaini | 293 – 644 | CytoplasmicSequence analysisAdd BLAST | 352 | |
Transmembranei | 645 – 666 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 667 – 671 | ExtracellularSequence analysis | 5 | |
Transmembranei | 672 – 694 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 695 – 710 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 711 – 731 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 732 – 752 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 753 – 773 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 774 – 785 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 786 – 806 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 807 – 814 | ExtracellularSequence analysis | 8 | |
Transmembranei | 815 – 835 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 836 – 949 | CytoplasmicSequence analysisAdd BLAST | 114 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 947 | Y → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication | 1 | |
Mutagenesisi | 948 | T → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication | 1 | |
Mutagenesisi | 949 | V → A: Impaired fusicoccin- (FC) dependent activation by 14-3-3 protein. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046276 | 1 – 949 | ATPase 3, plasma membrane-typeAdd BLAST | 949 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 882 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 932 | PhosphoserineBy similarity | 1 | |
Modified residuei | 948 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Phosphorylation level varies significantly during early response to bacterial elicitor (e.g. fusicoccin FC).1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P20431 |
PRIDEi | P20431 |
ProteomicsDBi | 234981 |
PTM databases
iPTMneti | P20431 |
Expressioni
Tissue specificityi
Found predominantly in phloem cells of leaves, stems, roots and flowers.
Gene expression databases
ExpressionAtlasi | P20431, baseline and differential |
Genevisiblei | P20431, AT |
Interactioni
Subunit structurei
Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-948. Binding to 14-3-3 proteins activates the H+-ATPase.
Protein-protein interaction databases
BioGRIDi | 21085, 5 interactors |
STRINGi | 3702.AT5G57350.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 947 – 949 | Interaction with 14-3-3 proteins | 3 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_4_1 |
InParanoidi | P20431 |
OMAi | LTEGANR |
OrthoDBi | 188115at2759 |
PhylomeDBi | P20431 |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P20431-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASGLEDIVN ENVDLEKIPI EEVFQQLKCS REGLSGAEGE NRLQIFGPNK
60 70 80 90 100
LEEKKESKLL KFLGFMWNPL SWVMEAAAIM AIALANGGGK PPDWQDFVGI
110 120 130 140 150
VCLLVINSTI SFVEENNAGN AAAALMAGLA PKTKVLRDGK WSEQEASILV
160 170 180 190 200
PGDIVSIKLG DIIPADARLL EGDPLKVDQS ALTGESLPAT KGPGEEVFSG
210 220 230 240 250
STCKQGEIEA VVIATGVHTF FGKAAHLVDS TNQVGHFQKV LTAIGNFCIC
260 270 280 290 300
SIAVGIAIEI VVMYPIQRRH YRDGIDNLLV LLIGGIPIAM PTVLSVTMAI
310 320 330 340 350
GSHKLSQQGA ITKRMTAIEE MAGMDVLCSD KTGTLTLNKL SVDKNLIEVY
360 370 380 390 400
CKGVEKDEVL LFAARASRVE NQDAIDAAMV GMLADPKEAR AGIREIHFLP
410 420 430 440 450
FNPVDKRTAL TFIDSNGNWH RVSKGAPEQI LDLCNARADL RKRVHSTIDK
460 470 480 490 500
YAERGLRSLA VSRQTVPEKT KESSGSPWEF VGVLPLFDPP RHDSAETIRR
510 520 530 540 550
ALDLGVNVKM ITGDQLAIAK ETGRRLGMGS NMYPSSSLLG KHKDEAMAHI
560 570 580 590 600
PVEDLIEKAD GFAGVFPEHK YEIVKKLQER KHICGMTGDG VNDAPALKKA
610 620 630 640 650
DIGIAVADAT DAARGASDIV LTEPGLSVII SAVLTSRAIF QRMKNYTIYA
660 670 680 690 700
VSITIRIVFG FMLIALIWKF DFSPFMVLII AILNDGTIMT ISKDRVKPSP
710 720 730 740 750
TPDSWKLKEI FATGVVLGGY MAIMTVVFFW AAYKTDFFPR TFHVRDLRGS
760 770 780 790 800
EHEMMSALYL QVSIVSQALI FVTRSRSWSF TERPGYFLLI AFWVAQLIAT
810 820 830 840 850
AIAVYGNWEF ARIKGIGWGW AGVIWLYSIV FYFPLDIMKF AIRYILAGTA
860 870 880 890 900
WKNIIDNRTA FTTKQNYGIE EREAQWAHAQ RTLHGLQNTE TANVVPERGG
910 920 930 940
YRELSEIANQ AKRRAEIARL RELHTLKGHV ESVVKLKGLD IETAGHYTV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8BD43 | A0A1P8BD43_ARATH | Plasma membrane ATPase | HA3 AHA3, ATAHA3, H(+)-ATPase 3, MJB24.16, MJB24_16 | 687 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04737 Genomic DNA Translation: AAA32750.1 AB019233, AB016891 Genomic DNA Translation: BAB09963.1 CP002688 Genomic DNA Translation: AED96889.1 CP002688 Genomic DNA Translation: AED96890.1 CP002688 Genomic DNA Translation: ANM69534.1 AY072153 mRNA Translation: AAL59975.1 X60115 Genomic DNA Translation: CAA42714.1 |
PIRi | A33698, PXMUP3 |
RefSeqi | NP_001190559.1, NM_001203630.1 NP_001331204.1, NM_001345259.1 NP_200545.1, NM_125118.3 |
Genome annotation databases
EnsemblPlantsi | AT5G57350.1; AT5G57350.1; AT5G57350 AT5G57350.2; AT5G57350.2; AT5G57350 AT5G57350.4; AT5G57350.4; AT5G57350 |
GeneIDi | 835841 |
Gramenei | AT5G57350.1; AT5G57350.1; AT5G57350 AT5G57350.2; AT5G57350.2; AT5G57350 AT5G57350.4; AT5G57350.4; AT5G57350 |
KEGGi | ath:AT5G57350 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04737 Genomic DNA Translation: AAA32750.1 AB019233, AB016891 Genomic DNA Translation: BAB09963.1 CP002688 Genomic DNA Translation: AED96889.1 CP002688 Genomic DNA Translation: AED96890.1 CP002688 Genomic DNA Translation: ANM69534.1 AY072153 mRNA Translation: AAL59975.1 X60115 Genomic DNA Translation: CAA42714.1 |
PIRi | A33698, PXMUP3 |
RefSeqi | NP_001190559.1, NM_001203630.1 NP_001331204.1, NM_001345259.1 NP_200545.1, NM_125118.3 |
3D structure databases
SMRi | P20431 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 21085, 5 interactors |
STRINGi | 3702.AT5G57350.1 |
PTM databases
iPTMneti | P20431 |
Proteomic databases
PaxDbi | P20431 |
PRIDEi | P20431 |
ProteomicsDBi | 234981 |
Genome annotation databases
EnsemblPlantsi | AT5G57350.1; AT5G57350.1; AT5G57350 AT5G57350.2; AT5G57350.2; AT5G57350 AT5G57350.4; AT5G57350.4; AT5G57350 |
GeneIDi | 835841 |
Gramenei | AT5G57350.1; AT5G57350.1; AT5G57350 AT5G57350.2; AT5G57350.2; AT5G57350 AT5G57350.4; AT5G57350.4; AT5G57350 |
KEGGi | ath:AT5G57350 |
Organism-specific databases
Araporti | AT5G57350 |
TAIRi | locus:2165600, AT5G57350 |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_4_1 |
InParanoidi | P20431 |
OMAi | LTEGANR |
OrthoDBi | 188115at2759 |
PhylomeDBi | P20431 |
Enzyme and pathway databases
BioCyci | ARA:AT5G57350-MONOMER |
Miscellaneous databases
PROi | PR:P20431 |
Gene expression databases
ExpressionAtlasi | P20431, baseline and differential |
Genevisiblei | P20431, AT |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA3_ARATH | |
Accessioni | P20431Primary (citable) accession number: P20431 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 174 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families