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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

BioCyciBSUB:BSU01430-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:BSU01430
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001752651 – 314DNA-directed RNA polymerase subunit alphaAdd BLAST314

Proteomic databases

PaxDbiP20429
PRIDEiP20429

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

IntActiP20429, 4 interactors
MINTiP20429
STRINGi224308.Bsubs1_010100000740

Structurei

Secondary structure

1314
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20429
SMRiP20429
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20429

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 228Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST228
Regioni245 – 314Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST70

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTF Bacteria
COG0202 LUCA
HOGENOMiHOG000218480
InParanoidiP20429
KOiK03040
OMAiLMKFRNF
PhylomeDBiP20429

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit

Sequencei

Sequence statusi: Complete.

P20429-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIEIEKPKIE TVEISDDAKF GKFVVEPLER GYGTTLGNSL RRILLSSLPG
60 70 80 90 100
AAVTSIQIDG VLHEFSTIEG VVEDVTTIIL HIKKLALKIY SDEEKTLEID
110 120 130 140 150
VQGEGTVTAA DITHDSDVEI LNPDLHIATL GENASFRVRL TAQRGRGYTP
160 170 180 190 200
ADANKRDDQP IGVIPIDSIY TPVSRVSYQV ENTRVGQVAN YDKLTLDVWT
210 220 230 240 250
DGSTGPKEAI ALGSKILTEH LNIFVGLTDE AQHAEIMVEK EEDQKEKVLE
260 270 280 290 300
MTIEELDLSV RSYNCLKRAG INTVQELANK TEEDMMKVRN LGRKSLEEVK
310
AKLEELGLGL RKDD
Length:314
Mass (Da):34,799
Last modified:February 1, 1991 - v1
Checksum:i9ADCAD891C0BCD67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26414 Genomic DNA Translation: AAA22217.1
L47971 Genomic DNA Translation: AAB06826.1
AL009126 Genomic DNA Translation: CAB11919.1
M13957 Genomic DNA Translation: AAA22708.1
PIRiE32307
RefSeqiNP_388024.1, NC_000964.3
WP_003225835.1, NZ_JNCM01000029.1

Genome annotation databases

EnsemblBacteriaiCAB11919; CAB11919; BSU01430
GeneIDi938921
KEGGibsu:BSU01430
PATRICifig|224308.179.peg.147

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26414 Genomic DNA Translation: AAA22217.1
L47971 Genomic DNA Translation: AAB06826.1
AL009126 Genomic DNA Translation: CAB11919.1
M13957 Genomic DNA Translation: AAA22708.1
PIRiE32307
RefSeqiNP_388024.1, NC_000964.3
WP_003225835.1, NZ_JNCM01000029.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Z3EX-ray1.50B245-314[»]
3GFKX-ray2.30B240-314[»]
3IHQX-ray1.90B245-314[»]
ProteinModelPortaliP20429
SMRiP20429
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP20429, 4 interactors
MINTiP20429
STRINGi224308.Bsubs1_010100000740

Proteomic databases

PaxDbiP20429
PRIDEiP20429

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB11919; CAB11919; BSU01430
GeneIDi938921
KEGGibsu:BSU01430
PATRICifig|224308.179.peg.147

Phylogenomic databases

eggNOGiENOG4105CTF Bacteria
COG0202 LUCA
HOGENOMiHOG000218480
InParanoidiP20429
KOiK03040
OMAiLMKFRNF
PhylomeDBiP20429

Enzyme and pathway databases

BioCyciBSUB:BSU01430-MONOMER

Miscellaneous databases

EvolutionaryTraceiP20429
PROiPR:P20429

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPOA_BACSU
AccessioniPrimary (citable) accession number: P20429
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 25, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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