UniProtKB - P20428 (MYOG_RAT)
Myogenin
Myog
Functioni
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.
1 PublicationGO - Molecular functioni
- chromatin DNA binding Source: UniProtKB
- cis-regulatory region sequence-specific DNA binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- E-box binding Source: UniProtKB
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: RGD
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: RGD
- sequence-specific DNA binding Source: RGD
- sequence-specific double-stranded DNA binding Source: RGD
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cellular response to estradiol stimulus Source: UniProtKB
- cellular response to growth factor stimulus Source: RGD
- cellular response to lithium ion Source: RGD
- cellular response to magnetism Source: RGD
- cellular response to retinoic acid Source: RGD
- cellular response to tumor necrosis factor Source: RGD
- muscle cell differentiation Source: RGD
- muscle organ development Source: RGD
- myoblast differentiation Source: RGD
- myotube differentiation Source: RGD
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of glycolytic process Source: RGD
- ossification Source: RGD
- positive regulation of muscle atrophy Source: UniProtKB
- positive regulation of myoblast differentiation Source: UniProtKB
- positive regulation of myotube differentiation Source: UniProtKB
- positive regulation of oxidative phosphorylation Source: RGD
- positive regulation of skeletal muscle fiber development Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: RGD
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of cell cycle Source: UniProtKB
- regulation of myoblast fusion Source: UniProtKB
- regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to denervation involved in regulation of muscle adaptation Source: UniProtKB
- response to electrical stimulus involved in regulation of muscle adaptation Source: UniProtKB
- response to gravity Source: RGD
- response to muscle activity Source: RGD
- response to muscle activity involved in regulation of muscle adaptation Source: UniProtKB
- skeletal muscle atrophy Source: RGD
- skeletal muscle cell differentiation Source: GO_Central
- skeletal muscle fiber development Source: RGD
- skeletal muscle tissue development Source: RGD
- skeletal muscle tissue regeneration Source: RGD
- striated muscle atrophy Source: UniProtKB
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Cell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-RNO-525793, Myogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Myogenin |
Gene namesi | Name:Myog |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620432, Myog |
Subcellular locationi
Nucleus
Note: Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.By similarity
Nucleus
- nucleus Source: UniProtKB
Other locations
- protein-DNA complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 77 | S → A: Reduces phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 79 | S → A: Reduces phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 87 | T → A: Reduces phosphorylation and inhibits DNA-binding and transcriptional activation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127378 | 1 – 287 | MyogeninAdd BLAST | 287 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 77 | Phosphoserine; by CaMK2G1 Publication | 1 | |
Modified residuei | 79 | Phosphoserine; by CaMK2G1 Publication | 1 | |
Modified residuei | 87 | Phosphothreonine; by CaMK2G1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P20428 |
PTM databases
iPTMneti | P20428 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSRNOG00000030743, Expressed in quadriceps femoris and 10 other tissues |
ExpressionAtlasi | P20428, baseline and differential |
Genevisiblei | P20428, RN |
Interactioni
Subunit structurei
Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities.
Interacts with SMARCA4/BRG1/BAF190A.
Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex (By similarity).
Interacts with CSRP3.
By similarity1 PublicationGO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
DIPi | DIP-52N |
STRINGi | 10116.ENSRNOP00000039817 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 132 | bHLHPROSITE-ProRule annotationAdd BLAST | 52 |
Phylogenomic databases
eggNOGi | KOG3960, Eukaryota |
GeneTreei | ENSGT00950000182959 |
HOGENOMi | CLU_969646_0_0_1 |
InParanoidi | P20428 |
OMAi | KPCPTEI |
OrthoDBi | 1471470at2759 |
PhylomeDBi | P20428 |
TreeFami | TF316344 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002546, MyoD_N IPR039704, Myogenic_factor |
PANTHERi | PTHR11534, PTHR11534, 1 hit |
Pfami | View protein in Pfam PF01586, Basic, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00520, BASIC, 1 hit SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL
60 70 80 90 100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA
110 120 130 140 150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG
160 170 180 190 200
GGGPSRWYPV NATPTAPPAV RSGAMHWSLV PTQEIICSQL TLQVPTTCTP
210 220 230 240 250
LRPSWTASRW RICLSPSQMK PCPTEIVCQA GCAWEPLSWC QTPPLLQQGP
260 270 280
FKWGCPGAQK TALGCHKPDY PPSIHIRLTP SPAREFN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketO70425 | O70425_RAT | Myogenin | Myog rCG_46454 | 224 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24393 mRNA Translation: AAA41662.1 |
PIRi | A31876 |
RefSeqi | NP_058811.2, NM_017115.2 |
Genome annotation databases
GeneIDi | 29148 |
KEGGi | rno:29148 |
UCSCi | RGD:620432, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24393 mRNA Translation: AAA41662.1 |
PIRi | A31876 |
RefSeqi | NP_058811.2, NM_017115.2 |
3D structure databases
AlphaFoldDBi | P20428 |
SMRi | P20428 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-52N |
STRINGi | 10116.ENSRNOP00000039817 |
PTM databases
iPTMneti | P20428 |
Proteomic databases
PaxDbi | P20428 |
Genome annotation databases
GeneIDi | 29148 |
KEGGi | rno:29148 |
UCSCi | RGD:620432, rat |
Organism-specific databases
CTDi | 4656 |
RGDi | 620432, Myog |
Phylogenomic databases
eggNOGi | KOG3960, Eukaryota |
GeneTreei | ENSGT00950000182959 |
HOGENOMi | CLU_969646_0_0_1 |
InParanoidi | P20428 |
OMAi | KPCPTEI |
OrthoDBi | 1471470at2759 |
PhylomeDBi | P20428 |
TreeFami | TF316344 |
Enzyme and pathway databases
Reactomei | R-RNO-525793, Myogenesis |
Miscellaneous databases
PROi | PR:P20428 |
Gene expression databases
Bgeei | ENSRNOG00000030743, Expressed in quadriceps femoris and 10 other tissues |
ExpressionAtlasi | P20428, baseline and differential |
Genevisiblei | P20428, RN |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR002546, MyoD_N IPR039704, Myogenic_factor |
PANTHERi | PTHR11534, PTHR11534, 1 hit |
Pfami | View protein in Pfam PF01586, Basic, 1 hit PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00520, BASIC, 1 hit SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MYOG_RAT | |
Accessioni | P20428Primary (citable) accession number: P20428 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |