Myog

Functionⁱ

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.1 Publication

GO - Molecular functionⁱ

chromatin DNA binding Source: UniProtKB
DNA-binding transcription factor activity Source: UniProtKB
E-box binding Source: UniProtKB
protein dimerization activity Source: InterPro
RNA polymerase II proximal promoter sequence-specific DNA binding
Source: RGD
Inferred from direct assayⁱ
- 14751541
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: GO_Central
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell cycle Source: UniProtKB-KW
cellular response to estradiol stimulus Source: UniProtKB
cellular response to magnetism
Source: RGD
Inferred from expression patternⁱ
- 10949997
cellular response to retinoic acid
Source: RGD
Inferred from expression patternⁱ
- 1323566
myoblast differentiation
Source: RGD
Inferred from expression patternⁱ
- 8853901
myotube differentiation
Source: RGD
Inferred from mutant phenotypeⁱ
- 1696254
negative regulation of cell proliferation Source: UniProtKB
negative regulation of glycolytic process
Source: RGD
Inferred from direct assayⁱ
- 10225962
positive regulation of cell cycle arrest Source: UniProtKB
positive regulation of muscle atrophy Source: UniProtKB
positive regulation of myoblast differentiation Source: UniProtKB
positive regulation of myoblast fusion Source: GO_Central
positive regulation of myotube differentiation Source: UniProtKB
positive regulation of oxidative phosphorylation
Source: RGD
Inferred from direct assayⁱ
- 10225962
positive regulation of skeletal muscle fiber development Source: UniProtKB
positive regulation of transcription by RNA polymerase II
Source: UniProtKB
Inferred from direct assayⁱ
- 14751541
regulation of myoblast fusion Source: UniProtKB
regulation of skeletal muscle satellite cell proliferation Source: UniProtKB
response to denervation involved in regulation of muscle adaptation Source: UniProtKB
response to electrical stimulus involved in regulation of muscle adaptation
Source: UniProtKB
Inferred from direct assayⁱ
- 21465538
response to gravity
Source: RGD
Inferred from expression patternⁱ
- 11960943
response to muscle activity
Source: RGD
Inferred from expression patternⁱ
- 15033961
response to muscle activity involved in regulation of muscle adaptation
Source: UniProtKB
Inferred from direct assayⁱ
- 14751541
skeletal muscle atrophy
Source: RGD
Inferred from expression patternⁱ
- 12839833
skeletal muscle cell differentiation Source: GO_Central
skeletal muscle tissue regeneration
Source: RGD
Inferred from expression patternⁱ
- 10867795
striated muscle atrophy Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Developmental protein, DNA-binding
Biological process	Cell cycle, Differentiation, Myogenesis, Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-RNO-375170 CDO in myogenesis

Reactomeⁱ

R-RNO-375170 CDO in myogenesis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Myogenin
Gene namesⁱ	Name:Myog
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 13

Organism-specific databases

Rat genome database More...RGDⁱ	620432 Myog

RGDⁱ

620432 Myog

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	77	S → A: Reduces phosphorylation. 1 Publication	1
Mutagenesisⁱ	79	S → A: Reduces phosphorylation. 1 Publication	1
Mutagenesisⁱ	87	T → A: Reduces phosphorylation and inhibits DNA-binding and transcriptional activation. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000127378	1 – 287	MyogeninAdd BLAST		287

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	77	Phosphoserine; by CaMK2G1 Publication	1
Modified residueⁱ	79	Phosphoserine; by CaMK2G1 Publication	1
Modified residueⁱ	87	Phosphothreonine; by CaMK2G1 Publication	1

Post-translational modificationⁱ

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles.1 Publication

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	P20428
PRIDEⁱ	P20428

PTM databases

iPTMnetⁱ	P20428

iPTMnetⁱ

P20428

Expressionⁱ

Tissue specificityⁱ

Expressed in muscle (at protein level).

Inductionⁱ

Up-regulated in denerved muscles cells. Down-regulated in soleus muscle in an electrical activity-dependent manner.1 Publication

Gene expression databases

Bgeeⁱ	ENSRNOG00000030743 Expressed in 4 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasⁱ	P20428 baseline and differential
Genevisibleⁱ	P20428 RN

Interactionⁱ

Subunit structureⁱ

Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminal region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex (By similarity). Interacts with CSRP3.By similarity1 Publication

GO - Molecular functionⁱ

protein dimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-52N
STRINGⁱ	10116.ENSRNOP00000039817

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P20428
SMRⁱ	P20428
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	81 – 132	bHLHPROSITE-ProRule annotationAdd BLAST		52

Phylogenomic databases

eggNOGⁱ	KOG3960 Eukaryota ENOG4111SED LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063004
HOGENOMⁱ	HOG000234799
InParanoidⁱ	P20428
KEGG Orthology (KO) More...KOⁱ	K18483
OMAⁱ	PCPTEIV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0GKD
PhylomeDBⁱ	P20428
TreeFamⁱ	TF316344

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR002546 Basic IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01586 Basic, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00520 BASIC, 1 hit SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P20428-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MELYETSPYF YQEPHFYDGE NYLPVHLQGF EPPGYERTEL SLSPEARGPL 
        60         70         80         90        100
EEKGLGTPEH CPGQCLPWAC KVCKRKSVSV DRRRAATLRE KRRLKKVNEA 
       110        120        130        140        150
FEALKRSTLL NPNQRLPKVE ILRSAIQYIE RLQALLSSLN QEERDLRYRG 
       160        170        180        190        200
GGGPSRWYPV NATPTAPPAV RSGAMHWSLV PTQEIICSQL TLQVPTTCTP 
       210        220        230        240        250
LRPSWTASRW RICLSPSQMK PCPTEIVCQA GCAWEPLSWC QTPPLLQQGP 
       260        270        280 
FKWGCPGAQK TALGCHKPDY PPSIHIRLTP SPAREFN

Length:287

Mass (Da):32,503

Last modified:February 1, 1991 - v1

Checksum:ⁱBE454E59B1464B40

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	O70425	O70425_RAT		Myogenin Myogenin	Myog rCG_46454	224	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M24393 mRNA Translation: AAA41662.1
PIRⁱ	A31876
NCBI Reference Sequences More...RefSeqⁱ	NP_058811.2, NM_017115.2
UniGeneⁱ	Rn.9465

Genome annotation databases

Ensemblⁱ	ENSRNOT00000087240; ENSRNOP00000073308; ENSRNOG00000030743
GeneIDⁱ	29148
KEGGⁱ	rno:29148
UCSC genome browser More...UCSCⁱ	RGD:620432 rat

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P20428	Myogenin		MOUSE		224	UniRef50_P12979
Myogenin		HUMAN		224
Myogenin		PIG		224
Myogenin		BOVIN		224
Myogenin		CHICK		227
+184

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	M24393 mRNA Translation: AAA41662.1
PIRⁱ	A31876
RefSeqⁱ	NP_058811.2, NM_017115.2
UniGeneⁱ	Rn.9465

3D structure databases

ProteinModelPortalⁱ	P20428
SMRⁱ	P20428
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

DIPⁱ	DIP-52N
STRINGⁱ	10116.ENSRNOP00000039817

PTM databases

iPTMnetⁱ	P20428

iPTMnetⁱ

P20428

Proteomic databases

PaxDbⁱ	P20428
PRIDEⁱ	P20428

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000087240; ENSRNOP00000073308; ENSRNOG00000030743
GeneIDⁱ	29148
KEGGⁱ	rno:29148
UCSCⁱ	RGD:620432 rat

Organism-specific databases

CTDⁱ	4656
RGDⁱ	620432 Myog

Phylogenomic databases

eggNOGⁱ	KOG3960 Eukaryota ENOG4111SED LUCA
GeneTreeⁱ	ENSGT00530000063004
HOGENOMⁱ	HOG000234799
InParanoidⁱ	P20428
KOⁱ	K18483
OMAⁱ	PCPTEIV
OrthoDBⁱ	EOG091G0GKD
PhylomeDBⁱ	P20428
TreeFamⁱ	TF316344

Enzyme and pathway databases

Reactomeⁱ	R-RNO-375170 CDO in myogenesis

Reactomeⁱ

R-RNO-375170 CDO in myogenesis

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P20428

PROⁱ

PR:P20428

Gene expression databases

Bgeeⁱ	ENSRNOG00000030743 Expressed in 4 organ(s), highest expression level in skeletal muscle tissue
ExpressionAtlasⁱ	P20428 baseline and differential
Genevisibleⁱ	P20428 RN

Family and domain databases

CDDⁱ	cd00083 HLH, 1 hit
Gene3Dⁱ	4.10.280.10, 1 hit
InterProⁱ	View protein in InterPro IPR002546 Basic IPR011598 bHLH_dom IPR036638 HLH_DNA-bd_sf
Pfamⁱ	View protein in Pfam PF01586 Basic, 1 hit PF00010 HLH, 1 hit
SMARTⁱ	View protein in SMART SM00520 BASIC, 1 hit SM00353 HLH, 1 hit
SUPFAMⁱ	SSF47459 SSF47459, 1 hit
PROSITEⁱ	View protein in PROSITE PS50888 BHLH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MYOG_RAT
Accessionⁱ	P20428Primary (citable) accession number: P20428
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	February 1, 1991
	Last modified:	September 12, 2018
	This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P20428 (MYOG_RAT)

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Myogenin

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ