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Entry version 183 (11 Dec 2019)
Sequence version 2 (01 Oct 1996)
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Protein

Signal recognition particle subunit SRP54

Gene

SRP54

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPAse-activating proteins (GAPs), thereby resolving their association.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi116 – 123GTPBy similarity8
Nucleotide bindingi198 – 202GTPBy similarity5
Nucleotide bindingi256 – 259GTPBy similarity4

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionGTPase activation, Ribonucleoprotein, RNA-binding
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-34231-MONOMER

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Signal recognition particle subunit SRP54
Alternative name(s):
Signal recognition particle 54 kDa protein homolog
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SRP54
Synonyms:SRH1
Ordered Locus Names:YPR088C
ORF Names:P9513.14
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome XVI

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YPR088C

Saccharomyces Genome Database

More...
SGDi
S000006292 SRP54

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Signal recognition particle

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001012031 – 541Signal recognition particle subunit SRP54Add BLAST541

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P20424

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P20424

PRoteomics IDEntifications database

More...
PRIDEi
P20424

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P20424

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. SRP54 interacts with SRP101.

1 Publication

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
36258, 274 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-609 Signal recognition particle

Database of interacting proteins

More...
DIPi
DIP-2943N

Protein interaction database and analysis system

More...
IntActi
P20424, 23 interactors

Molecular INTeraction database

More...
MINTi
P20424

STRING: functional protein association networks

More...
STRINGi
4932.YPR088C

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...
RNActi
P20424 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P20424

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 303G-domainAdd BLAST303
Regioni304 – 541M-domainAdd BLAST238

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA and also binds the signal sequence.

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the GTP-binding SRP family. SRP54 subfamily.Curated

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000036165

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P20424

KEGG Orthology (KO)

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KOi
K03106

Identification of Orthologs from Complete Genome Data

More...
OMAi
DTAGRHK

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.260.30, 1 hit
1.20.120.140, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00306 SRP54, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR036225 SRP/SRP_N
IPR022941 SRP54
IPR006325 SRP54_euk
IPR000897 SRP54_GTPase_dom
IPR042101 SRP54_N_sf

The PANTHER Classification System

More...
PANTHERi
PTHR11564 PTHR11564, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47364 SSF47364, 1 hit
SSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01425 SRP54_euk, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00300 SRP54, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P20424-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVLADLGKRI NSAVNNAISN TQDDFTTSVD VMLKGIVTAL LESDVNIALV
60 70 80 90 100
SKLRNNIRSQ LLSENRSEKS TTNAQTKKLI QKTVFDELCK LVTCEGSEEK
110 120 130 140 150
AFVPKKRKTN IIMFVGLQGS GKTTSCTKLA VYYSKRGFKV GLVCADTFRA
160 170 180 190 200
GAFDQLKQNA IRARIPFYGS YTETDPAKVA EEGINKFKKE KFDIIIVDTS
210 220 230 240 250
GRHHQEEELF QEMIEISNVI KPNQTIMVLD ASIGQAAEQQ SKAFKESSDF
260 270 280 290 300
GAIILTKMDG HARGGGAISA VAATNTPIIF IGTGEHIHDL EKFSPKSFIS
310 320 330 340 350
KLLGIGDIES LFEQLQTVSN KEDAKATMEN IQKGKFTLLD FKKQMQTIMK
360 370 380 390 400
MGPLSNIAQM IPGMSNMMNQ VGEEETSQKM KKMVYVLDSM TKEELESDGR
410 420 430 440 450
MFIEEPTRMV RVAKGSGTSV FEVEMILMQQ QMMARMAQTA TQQQPGAPGA
460 470 480 490 500
NARMPGMPNM PGMPNMPGMP NMPGMPKVTP QMMQQAQQKL KQNPGLMQNM
510 520 530 540
MNMFGGGMGG GMGGGMPDMN EMMKMMQDPQ MQQMAKQFGM G
Length:541
Mass (Da):59,624
Last modified:October 1, 1996 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i05B1B2C262932F17
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti136R → E in CAA34781 (PubMed:2187859).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X16908 Genomic DNA Translation: CAA34781.1
M55517 Genomic DNA Translation: AAA35092.1
X51614 Genomic DNA Translation: CAA35952.1
U51033 Genomic DNA Translation: AAB68136.1
BK006949 Genomic DNA Translation: DAA11505.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S69073 JX0112

NCBI Reference Sequences

More...
RefSeqi
NP_015413.1, NM_001184185.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YPR088C_mRNA; YPR088C; YPR088C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
856203

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YPR088C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16908 Genomic DNA Translation: CAA34781.1
M55517 Genomic DNA Translation: AAA35092.1
X51614 Genomic DNA Translation: CAA35952.1
U51033 Genomic DNA Translation: AAB68136.1
BK006949 Genomic DNA Translation: DAA11505.1
PIRiS69073 JX0112
RefSeqiNP_015413.1, NM_001184185.1

3D structure databases

SMRiP20424
ModBaseiSearch...

Protein-protein interaction databases

BioGridi36258, 274 interactors
ComplexPortaliCPX-609 Signal recognition particle
DIPiDIP-2943N
IntActiP20424, 23 interactors
MINTiP20424
STRINGi4932.YPR088C

PTM databases

iPTMnetiP20424

Proteomic databases

MaxQBiP20424
PaxDbiP20424
PRIDEiP20424

Genome annotation databases

EnsemblFungiiYPR088C_mRNA; YPR088C; YPR088C
GeneIDi856203
KEGGisce:YPR088C

Organism-specific databases

EuPathDBiFungiDB:YPR088C
SGDiS000006292 SRP54

Phylogenomic databases

HOGENOMiHOG000036165
InParanoidiP20424
KOiK03106
OMAiDTAGRHK

Enzyme and pathway databases

BioCyciYEAST:G3O-34231-MONOMER
ReactomeiR-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P20424
RNActiP20424 protein

Family and domain databases

Gene3Di1.10.260.30, 1 hit
1.20.120.140, 1 hit
HAMAPiMF_00306 SRP54, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR027417 P-loop_NTPase
IPR036891 Signal_recog_part_SRP54_M_sf
IPR013822 Signal_recog_particl_SRP54_hlx
IPR004125 Signal_recog_particle_SRP54_M
IPR036225 SRP/SRP_N
IPR022941 SRP54
IPR006325 SRP54_euk
IPR000897 SRP54_GTPase_dom
IPR042101 SRP54_N_sf
PANTHERiPTHR11564 PTHR11564, 1 hit
PfamiView protein in Pfam
PF00448 SRP54, 1 hit
PF02881 SRP54_N, 1 hit
PF02978 SRP_SPB, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00962 SRP54, 1 hit
SM00963 SRP54_N, 1 hit
SUPFAMiSSF47364 SSF47364, 1 hit
SSF47446 SSF47446, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01425 SRP54_euk, 1 hit
PROSITEiView protein in PROSITE
PS00300 SRP54, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSRP54_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P20424
Secondary accession number(s): D6W489
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: December 11, 2019
This is version 183 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
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