UniProtKB - P20424 (SRP54_YEAST)
Signal recognition particle subunit SRP54
SRP54
Functioni
Signal-recognition-particle (SRP) assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum (ER) membrane. SRP is required for the cotranslational protein translocation for ER import and preferentially recognizes strongly hydrophobic signal sequences. It is involved in targeting the nascent chain-ribosome (RNC) complex to the ER and is proposed to participate in the arrest of nascent chain elongation during membrane targeting. SRP54 binds to the signal sequence of presecretory protein when they emerge from the ribosomes. SRP54 interacts with the scR1 RNA and mediates the association of the resulting SRP-RNC complex with the signal recognition particle receptor (SR) via its alpha subunit SRP101. Both, SRP54 and SRP101, are locked in their GTP bound forms in the SRP-RNC-SR complex, which dissociates upon transferring the signal sequence to the protein-conducting channel (translocon). After signal sequence transfer, SRP54 and SRP101 act as reciprocal GTPAse-activating proteins (GAPs), thereby resolving their association.
2 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 116 – 123 | GTPBy similarity | 8 | |
Nucleotide bindingi | 198 – 202 | GTPBy similarity | 5 | |
Nucleotide bindingi | 256 – 259 | GTPBy similarity | 4 |
GO - Molecular functioni
- 7S RNA binding Source: SGD
- endoplasmic reticulum signal peptide binding Source: GO_Central
- GTPase activator activity Source: UniProtKB-KW
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
GO - Biological processi
- SRP-dependent cotranslational protein targeting to membrane Source: SGD
- SRP-dependent cotranslational protein targeting to membrane, translocation Source: GO_Central
Keywordsi
Molecular function | GTPase activation, Ribonucleoprotein, RNA-binding |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-SCE-1799339, SRP-dependent cotranslational protein targeting to membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Signal recognition particle subunit SRP54Alternative name(s): Signal recognition particle 54 kDa protein homolog |
Gene namesi | Name:SRP54 Synonyms:SRH1 Ordered Locus Names:YPR088C ORF Names:P9513.14 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000006292, SRP54 |
VEuPathDBi | FungiDB:YPR088C |
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: GO_Central
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Other locations
Keywords - Cellular componenti
Cytoplasm, Signal recognition particlePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101203 | 1 – 541 | Signal recognition particle subunit SRP54Add BLAST | 541 |
Proteomic databases
MaxQBi | P20424 |
PaxDbi | P20424 |
PRIDEi | P20424 |
PTM databases
iPTMneti | P20424 |
Interactioni
Subunit structurei
Fungal signal recognition particle (SRP) complex consists of a 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68, SRP54, SEC65, SRP21 and SRP14. SRP54 interacts with SRP101.
1 PublicationProtein-protein interaction databases
BioGRIDi | 36258, 289 interactors |
ComplexPortali | CPX-609, Signal recognition particle |
DIPi | DIP-2943N |
IntActi | P20424, 23 interactors |
MINTi | P20424 |
STRINGi | 4932.YPR088C |
Miscellaneous databases
RNActi | P20424, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 303 | G-domainAdd BLAST | 303 | |
Regioni | 304 – 541 | M-domainAdd BLAST | 238 | |
Regioni | 442 – 489 | DisorderedSequence analysisAdd BLAST | 48 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 460 – 474 | Pro residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0780, Eukaryota |
GeneTreei | ENSGT00550000074824 |
HOGENOMi | CLU_009301_6_1_1 |
InParanoidi | P20424 |
OMAi | DTAGRHK |
Family and domain databases
Gene3Di | 1.10.260.30, 1 hit 1.20.120.140, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00306, SRP54, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR036891, Signal_recog_part_SRP54_M_sf IPR013822, Signal_recog_particl_SRP54_hlx IPR004125, Signal_recog_particle_SRP54_M IPR036225, SRP/SRP_N IPR022941, SRP54 IPR006325, SRP54_euk IPR000897, SRP54_GTPase_dom IPR042101, SRP54_N_sf |
PANTHERi | PTHR11564, PTHR11564, 1 hit |
Pfami | View protein in Pfam PF00448, SRP54, 1 hit PF02881, SRP54_N, 1 hit PF02978, SRP_SPB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00962, SRP54, 1 hit SM00963, SRP54_N, 1 hit |
SUPFAMi | SSF47364, SSF47364, 1 hit SSF47446, SSF47446, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01425, SRP54_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00300, SRP54, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MVLADLGKRI NSAVNNAISN TQDDFTTSVD VMLKGIVTAL LESDVNIALV
60 70 80 90 100
SKLRNNIRSQ LLSENRSEKS TTNAQTKKLI QKTVFDELCK LVTCEGSEEK
110 120 130 140 150
AFVPKKRKTN IIMFVGLQGS GKTTSCTKLA VYYSKRGFKV GLVCADTFRA
160 170 180 190 200
GAFDQLKQNA IRARIPFYGS YTETDPAKVA EEGINKFKKE KFDIIIVDTS
210 220 230 240 250
GRHHQEEELF QEMIEISNVI KPNQTIMVLD ASIGQAAEQQ SKAFKESSDF
260 270 280 290 300
GAIILTKMDG HARGGGAISA VAATNTPIIF IGTGEHIHDL EKFSPKSFIS
310 320 330 340 350
KLLGIGDIES LFEQLQTVSN KEDAKATMEN IQKGKFTLLD FKKQMQTIMK
360 370 380 390 400
MGPLSNIAQM IPGMSNMMNQ VGEEETSQKM KKMVYVLDSM TKEELESDGR
410 420 430 440 450
MFIEEPTRMV RVAKGSGTSV FEVEMILMQQ QMMARMAQTA TQQQPGAPGA
460 470 480 490 500
NARMPGMPNM PGMPNMPGMP NMPGMPKVTP QMMQQAQQKL KQNPGLMQNM
510 520 530 540
MNMFGGGMGG GMGGGMPDMN EMMKMMQDPQ MQQMAKQFGM G
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 136 | R → E in CAA34781 (PubMed:2187859).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16908 Genomic DNA Translation: CAA34781.1 M55517 Genomic DNA Translation: AAA35092.1 X51614 Genomic DNA Translation: CAA35952.1 U51033 Genomic DNA Translation: AAB68136.1 BK006949 Genomic DNA Translation: DAA11505.1 |
PIRi | S69073, JX0112 |
RefSeqi | NP_015413.1, NM_001184185.1 |
Genome annotation databases
EnsemblFungii | YPR088C_mRNA; YPR088C; YPR088C |
GeneIDi | 856203 |
KEGGi | sce:YPR088C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X16908 Genomic DNA Translation: CAA34781.1 M55517 Genomic DNA Translation: AAA35092.1 X51614 Genomic DNA Translation: CAA35952.1 U51033 Genomic DNA Translation: AAB68136.1 BK006949 Genomic DNA Translation: DAA11505.1 |
PIRi | S69073, JX0112 |
RefSeqi | NP_015413.1, NM_001184185.1 |
3D structure databases
SMRi | P20424 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 36258, 289 interactors |
ComplexPortali | CPX-609, Signal recognition particle |
DIPi | DIP-2943N |
IntActi | P20424, 23 interactors |
MINTi | P20424 |
STRINGi | 4932.YPR088C |
PTM databases
iPTMneti | P20424 |
Proteomic databases
MaxQBi | P20424 |
PaxDbi | P20424 |
PRIDEi | P20424 |
Genome annotation databases
EnsemblFungii | YPR088C_mRNA; YPR088C; YPR088C |
GeneIDi | 856203 |
KEGGi | sce:YPR088C |
Organism-specific databases
SGDi | S000006292, SRP54 |
VEuPathDBi | FungiDB:YPR088C |
Phylogenomic databases
eggNOGi | KOG0780, Eukaryota |
GeneTreei | ENSGT00550000074824 |
HOGENOMi | CLU_009301_6_1_1 |
InParanoidi | P20424 |
OMAi | DTAGRHK |
Enzyme and pathway databases
Reactomei | R-SCE-1799339, SRP-dependent cotranslational protein targeting to membrane |
Miscellaneous databases
PROi | PR:P20424 |
RNActi | P20424, protein |
Family and domain databases
Gene3Di | 1.10.260.30, 1 hit 1.20.120.140, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00306, SRP54, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR036891, Signal_recog_part_SRP54_M_sf IPR013822, Signal_recog_particl_SRP54_hlx IPR004125, Signal_recog_particle_SRP54_M IPR036225, SRP/SRP_N IPR022941, SRP54 IPR006325, SRP54_euk IPR000897, SRP54_GTPase_dom IPR042101, SRP54_N_sf |
PANTHERi | PTHR11564, PTHR11564, 1 hit |
Pfami | View protein in Pfam PF00448, SRP54, 1 hit PF02881, SRP54_N, 1 hit PF02978, SRP_SPB, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00962, SRP54, 1 hit SM00963, SRP54_N, 1 hit |
SUPFAMi | SSF47364, SSF47364, 1 hit SSF47446, SSF47446, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01425, SRP54_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00300, SRP54, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SRP54_YEAST | |
Accessioni | P20424Primary (citable) accession number: P20424 Secondary accession number(s): D6W489 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | October 1, 1996 | |
Last modified: | February 23, 2022 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families