UniProtKB - P20340 (RAB6A_HUMAN)
Ras-related protein Rab-6A
RAB6A
Functioni
Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Involved in COPI-independent retrograde transport from the Golgi to the ER (PubMed:25962623).
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 28 | GTP3 Publications | 9 | |
Nucleotide bindingi | 68 – 72 | GTP3 Publications | 5 | |
Nucleotide bindingi | 126 – 129 | GTP3 Publications | 4 | |
Nucleotide bindingi | 156 – 158 | GTP3 Publications | 3 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
- myosin V binding Source: UniProtKB
- protein domain specific binding Source: BHF-UCL
GO - Biological processi
- antigen processing and presentation Source: UniProtKB
- early endosome to Golgi transport Source: UniProtKB
- intracellular protein transport Source: GO_Central
- intra-Golgi vesicle-mediated transport Source: GO_Central
- minus-end-directed organelle transport along microtubule Source: BHF-UCL
- peptidyl-cysteine methylation Source: MGI
- protein localization to Golgi apparatus Source: UniProtKB
- retrograde transport, endosome to Golgi Source: GO_Central
- retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: UniProtKB
Keywordsi
Biological process | ER-Golgi transport, Host-virus interaction, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P20340 |
Reactomei | R-HSA-1912420, Pre-NOTCH Processing in Golgi R-HSA-6798695, Neutrophil degranulation R-HSA-6811436, COPI-independent Golgi-to-ER retrograde traffic R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8854214, TBC/RABGAPs R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SignaLinki | P20340 |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein Rab-6AShort name: Rab-6 |
Gene namesi | Name:RAB6A Synonyms:RAB6 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9786, RAB6A |
MIMi | 179513, gene |
neXtProti | NX_P20340 |
VEuPathDBi | HostDB:ENSG00000175582 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 2 Publications; Lipid-anchor 1 Publication
Note: BICD2 facilitates its targeting to Golgi apparatus membrane.1 Publication
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Lipid-anchor Curated
Golgi apparatus
- Golgi apparatus membrane 1 Publication; Lipid-anchor Curated
Cytosol
- cytosol Source: BHF-UCL
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB
- trans-Golgi network Source: BHF-UCL
- trans-Golgi network membrane Source: Reactome
Plasma Membrane
- plasma membrane Source: Reactome
Other locations
- cytoplasmic vesicle Source: BHF-UCL
- endomembrane system Source: GO_Central
- endosome to plasma membrane transport vesicle Source: UniProtKB
- membrane Source: BHF-UCL
- secretory granule membrane Source: Reactome
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | T → N: Loss of APBA1-binding. No loss of RIC1- and RGP1-binding. 2 Publications | 1 | |
Mutagenesisi | 46 | I → E: Loss of RAB6IP1-binding. 1 Publication | 1 | |
Mutagenesisi | 72 | Q → L: Loss of GTPase activity. Interacts with APBA1. 4 Publications | 1 |
Organism-specific databases
DisGeNETi | 5870 |
OpenTargetsi | ENSG00000175582 |
PharmGKBi | PA34146 |
Miscellaneous databases
Pharosi | P20340, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105703 |
Genetic variation databases
BioMutai | RAB6A |
DMDMi | 131796 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000121112 | 2 – 208 | Ras-related protein Rab-6AAdd BLAST | 207 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources1 Publication | 1 | |
Modified residuei | 82 | O-AMP-tyrosine; by Legionella DrrA1 Publication | 1 | |
Modified residuei | 184 | PhosphoserineBy similarity | 1 | |
Lipidationi | 206 | S-geranylgeranyl cysteineBy similarity | 1 | |
Modified residuei | 208 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 208 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | P20340 |
jPOSTi | P20340 |
MassIVEi | P20340 |
MaxQBi | P20340 |
PaxDbi | P20340 |
PeptideAtlasi | P20340 |
PRIDEi | P20340 |
ProteomicsDBi | 27097 53752 [P20340-1] 53753 [P20340-2] 61249 |
TopDownProteomicsi | P20340-2 [P20340-2] |
PTM databases
iPTMneti | P20340 |
MetOSitei | P20340 |
PhosphoSitePlusi | P20340 |
SwissPalmi | P20340 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000175582, Expressed in frontal cortex and 219 other tissues |
ExpressionAtlasi | P20340, baseline and differential |
Genevisiblei | P20340, HS |
Organism-specific databases
HPAi | ENSG00000175582, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with BICDL1; leads to its accumulation in the pericentrosomal region (By similarity).
Interacts with SCYL1BP1.
Interacts with VSP52 and RABGAP1.
Interacts with GCC2 (via its GRIP domain).
Interacts with RAB6IP1 (via its RUN 1 domain).
Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL.
Isoform 1 interacts with BICD1.
Isoform 2 interacts with BICD1.
Isoform 1 interacts with BICD2.
Isoform 2 interacts with BICD2.
Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct.
Interacts with PIFO.
Interacts (GTP-bound) with APBA1/MINT1 isoform 2, also called Mint1_826, but not with isoform 1.
Interacts with RIC1 and RGP1; the interactions are direct with a preference for RAB6A-GDP.
By similarity16 Publications(Microbial infection) Interacts with human cytomegalovirus protein UL32.
1 PublicationBinary interactionsi
P20340
With | #Exp. | IntAct |
---|---|---|
BICD2 [Q8TD16] | 3 | EBI-1052826,EBI-2372628 |
GCC2 [Q8IWJ2] | 4 | EBI-1052826,EBI-1645320 |
OCRL [Q01968] | 12 | EBI-1052826,EBI-6148898 |
PMM1 [Q92871] | 4 | EBI-1052826,EBI-746784 |
Isoform 1 [P20340-1]
Isoform 2 [P20340-2]
GO - Molecular functioni
- myosin V binding Source: UniProtKB
- protein domain specific binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 111808, 97 interactors |
DIPi | DIP-39668N |
IntActi | P20340, 39 interactors |
MINTi | P20340 |
STRINGi | 9606.ENSP00000311449 |
Chemistry databases
BindingDBi | P20340 |
Miscellaneous databases
RNActi | P20340, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P20340 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20340 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 50 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0094, Eukaryota |
GeneTreei | ENSGT00940000154769 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | P20340 |
OMAi | DNQMIDV |
PhylomeDBi | P20340 |
TreeFami | TF300803 |
Family and domain databases
DisProti | DP02733 |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSTGGDFGNP LRKFKLVFLG EQSVGKTSLI TRFMYDSFDN TYQATIGIDF
60 70 80 90 100
LSKTMYLEDR TVRLQLWDTA GQERFRSLIP SYIRDSTVAV VVYDITNVNS
110 120 130 140 150
FQQTTKWIDD VRTERGSDVI IMLVGNKTDL ADKRQVSIEE GERKAKELNV
160 170 180 190 200
MFIETSAKAG YNVKQLFRRV AAALPGMEST QDRSREDMID IKLEKPQEQP
VSEGGCSC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5H3K7 | F5H3K7_HUMAN | Ras-related protein Rab-6A | RAB6A | 71 | Annotation score: | ||
F5GX61 | F5GX61_HUMAN | Ras-related protein Rab-6A | RAB6A | 87 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 189 | I → T in BAH13508 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046967 | 1 – 33 | Missing in isoform 4. 1 PublicationAdd BLAST | 33 | |
Alternative sequenceiVSP_045302 | 62 – 165 | Missing in isoform 3. 1 PublicationAdd BLAST | 104 | |
Alternative sequenceiVSP_005527 | 62 – 88 | VRLQL…RDSTV → IRLQLWDTAGQERFRSLIPS YIRDSAA in isoform 2 and isoform 4. 6 PublicationsAdd BLAST | 27 |
Sequence databases
Genome annotation databases
Ensembli | ENST00000310653; ENSP00000311449; ENSG00000175582 [P20340-2] ENST00000336083; ENSP00000336850; ENSG00000175582 ENST00000541588; ENSP00000445350; ENSG00000175582 [P20340-3] ENST00000541973; ENSP00000443782; ENSG00000175582 [P20340-4] |
GeneIDi | 5870 |
KEGGi | hsa:5870 |
MANE-Selecti | ENST00000336083.8; ENSP00000336850.3; NM_198896.2; NP_942599.1 |
UCSCi | uc001oue.4, human [P20340-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YZQ | X-ray | 1.78 | A | 10-177 | [»] | |
2GIL | X-ray | 1.82 | A/B/C/D | 13-174 | [»] | |
3BBP | X-ray | 3.00 | A/B/C | 1-206 | [»] | |
3CWZ | X-ray | 3.20 | A | 8-195 | [»] | |
4DKX | X-ray | 1.90 | A/B | 1-208 | [»] | |
5LEF | X-ray | 2.09 | A/B | 8-195 | [»] | |
SMRi | P20340 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111808, 97 interactors |
DIPi | DIP-39668N |
IntActi | P20340, 39 interactors |
MINTi | P20340 |
STRINGi | 9606.ENSP00000311449 |
Chemistry databases
BindingDBi | P20340 |
ChEMBLi | CHEMBL4105703 |
PTM databases
iPTMneti | P20340 |
MetOSitei | P20340 |
PhosphoSitePlusi | P20340 |
SwissPalmi | P20340 |
Genetic variation databases
BioMutai | RAB6A |
DMDMi | 131796 |
Proteomic databases
EPDi | P20340 |
jPOSTi | P20340 |
MassIVEi | P20340 |
MaxQBi | P20340 |
PaxDbi | P20340 |
PeptideAtlasi | P20340 |
PRIDEi | P20340 |
ProteomicsDBi | 27097 53752 [P20340-1] 53753 [P20340-2] 61249 |
TopDownProteomicsi | P20340-2 [P20340-2] |
Protocols and materials databases
ABCDi | P20340, 1 sequenced antibody |
Antibodypediai | 4093, 301 antibodies from 33 providers |
DNASUi | 5870 |
Genome annotation databases
Ensembli | ENST00000310653; ENSP00000311449; ENSG00000175582 [P20340-2] ENST00000336083; ENSP00000336850; ENSG00000175582 ENST00000541588; ENSP00000445350; ENSG00000175582 [P20340-3] ENST00000541973; ENSP00000443782; ENSG00000175582 [P20340-4] |
GeneIDi | 5870 |
KEGGi | hsa:5870 |
MANE-Selecti | ENST00000336083.8; ENSP00000336850.3; NM_198896.2; NP_942599.1 |
UCSCi | uc001oue.4, human [P20340-1] |
Organism-specific databases
CTDi | 5870 |
DisGeNETi | 5870 |
GeneCardsi | RAB6A |
HGNCi | HGNC:9786, RAB6A |
HPAi | ENSG00000175582, Low tissue specificity |
MIMi | 179513, gene |
neXtProti | NX_P20340 |
OpenTargetsi | ENSG00000175582 |
PharmGKBi | PA34146 |
VEuPathDBi | HostDB:ENSG00000175582 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0094, Eukaryota |
GeneTreei | ENSGT00940000154769 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | P20340 |
OMAi | DNQMIDV |
PhylomeDBi | P20340 |
TreeFami | TF300803 |
Enzyme and pathway databases
PathwayCommonsi | P20340 |
Reactomei | R-HSA-1912420, Pre-NOTCH Processing in Golgi R-HSA-6798695, Neutrophil degranulation R-HSA-6811436, COPI-independent Golgi-to-ER retrograde traffic R-HSA-6811440, Retrograde transport at the Trans-Golgi-Network R-HSA-8854214, TBC/RABGAPs R-HSA-8873719, RAB geranylgeranylation R-HSA-8876198, RAB GEFs exchange GTP for GDP on RABs |
SignaLinki | P20340 |
Miscellaneous databases
BioGRID-ORCSi | 5870, 321 hits in 986 CRISPR screens |
ChiTaRSi | RAB6A, human |
EvolutionaryTracei | P20340 |
GeneWikii | RAB6A |
GenomeRNAii | 5870 |
Pharosi | P20340, Tbio |
PROi | PR:P20340 |
RNActi | P20340, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000175582, Expressed in frontal cortex and 219 other tissues |
ExpressionAtlasi | P20340, baseline and differential |
Genevisiblei | P20340, HS |
Family and domain databases
DisProti | DP02733 |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB6A_HUMAN | |
Accessioni | P20340Primary (citable) accession number: P20340 Secondary accession number(s): A8K133 Q9UBE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 235 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 11
Human chromosome 11: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families