UniProtKB - P20338 (RAB4A_HUMAN)
Ras-related protein Rab-4A
RAB4A
Functioni
Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state (PubMed:15907487, PubMed:16034420).
Involved in protein transport (PubMed:29425100).
Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling (PubMed:29425100).
Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets (By similarity).
By similarity3 PublicationsCaution
Catalytic activityi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 28 | GTP1 Publication | 9 | |
Nucleotide bindingi | 68 – 72 | GTP1 Publication | 5 | |
Nucleotide bindingi | 126 – 129 | GTP1 Publication | 4 | |
Nucleotide bindingi | 156 – 158 | GTP1 Publication | 3 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- G protein activity Source: UniProtKB-EC
- GTPase activity Source: UniProtKB
- GTP binding Source: UniProtKB
GO - Biological processi
- antigen processing and presentation Source: UniProtKB
- protein transport Source: UniProtKB-KW
- Rab protein signal transduction Source: InterPro
- regulation of endocytosis Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P20338 |
Reactomei | R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-1660499, Synthesis of PIPs at the plasma membrane R-HSA-8854214, TBC/RABGAPs R-HSA-8873719, RAB geranylgeranylation R-HSA-8875656, MET receptor recycling |
SignaLinki | P20338 |
SIGNORi | P20338 |
Protein family/group databases
MoonDBi | P20338, Predicted |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RAB4A Synonyms:RAB4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9781, RAB4A |
MIMi | 179511, gene |
neXtProti | NX_P20338 |
VEuPathDBi | HostDB:ENSG00000168118 |
Subcellular locationi
Endosome
- Early endosome membrane By similarity; Peripheral membrane protein By similarity
- Recycling endosome membrane By similarity; Peripheral membrane protein By similarity
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- Membrane ; Peripheral membrane protein 1 Publication
Note: Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.1 Publication
Endosome
- early endosome membrane Source: Reactome
- endosome Source: GO_Central
- postsynaptic recycling endosome Source: GO_Central
- recycling endosome membrane Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- cytoplasmic vesicle membrane Source: Reactome
- insulin-responsive compartment Source: GO_Central
- perinuclear region of cytoplasm Source: UniProtKB
- vesicle Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 51 | G → A: 10-fold decrease in ZFYVE20 binding affinity. 1 Publication | 1 | |
Mutagenesisi | 51 | G → L: 10-fold decrease in ZFYVE20 binding affinity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5867 |
OpenTargetsi | ENSG00000168118 |
PharmGKBi | PA34141 |
Miscellaneous databases
Pharosi | P20338, Tbio |
Genetic variation databases
BioMutai | RAB4A |
DMDMi | 460018296 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121093 | 1 – 218 | Ras-related protein Rab-4AAdd BLAST | 218 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 72 | 5-glutamyl serotoninBy similarity | 1 | |
Modified residuei | 190 | PhosphoserineCombined sources | 1 | |
Modified residuei | 204 | Phosphoserine; by CDK11 Publication | 1 | |
Lipidationi | 216 | S-geranylgeranyl cysteineBy similarity | 1 | |
Modified residuei | 218 | Cysteine methyl esterBy similarity | 1 | |
Lipidationi | 218 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Methylation, Phosphoprotein, PrenylationProteomic databases
EPDi | P20338 |
jPOSTi | P20338 |
MassIVEi | P20338 |
MaxQBi | P20338 |
PaxDbi | P20338 |
PeptideAtlasi | P20338 |
PRIDEi | P20338 |
ProteomicsDBi | 53750 |
PTM databases
iPTMneti | P20338 |
PhosphoSitePlusi | P20338 |
Expressioni
Gene expression databases
Bgeei | ENSG00000168118, Expressed in vagina and 251 other tissues |
ExpressionAtlasi | P20338, baseline and differential |
Genevisiblei | P20338, HS |
Organism-specific databases
HPAi | ENSG00000168118, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with SGSM1, SGSM2 and SGSM3 (By similarity).
Interacts with RAB11FIP1, RABEP1, ZFYVE20 and RUFY1 (PubMed:10698684, PubMed:11786538, PubMed:11788822, PubMed:14617813, PubMed:15280022, PubMed:16034420).
Interacts (membrane-bound form) with NDRG1; the interaction involves NDRG1 in vesicular recycling of E-cadherin (PubMed:17786215).
Interacts (in GTP-bound form) with GRIPAP1 (via N-terminus) (PubMed:20098723).
Interacts with RABEP1 and RBSN (PubMed:20098723). Does not interact with HPS4 (By similarity).
Interacts with RABEP2; this interaction may mediate VEGFR2 cell surface expression (PubMed:29425100).
By similarity10 PublicationsBinary interactionsi
P20338
Protein-protein interaction databases
BioGRIDi | 111805, 379 interactors |
DIPi | DIP-372N |
IntActi | P20338, 31 interactors |
MINTi | P20338 |
STRINGi | 9606.ENSP00000355651 |
Miscellaneous databases
RNActi | P20338, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P20338 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20338 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 42 – 50 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0086, Eukaryota |
GeneTreei | ENSGT00940000157464 |
HOGENOMi | CLU_041217_23_1_1 |
InParanoidi | P20338 |
OMAi | STQECGC |
OrthoDBi | 1146851at2759 |
PhylomeDBi | P20338 |
TreeFami | TF300032 |
Family and domain databases
CDDi | cd04113, Rab4, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041819, Rab4 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSQTAMSETY DFLFKFLVIG NAGTGKSCLL HQFIEKKFKD DSNHTIGVEF
60 70 80 90 100
GSKIINVGGK YVKLQIWDTA GQERFRSVTR SYYRGAAGAL LVYDITSRET
110 120 130 140 150
YNALTNWLTD ARMLASQNIV IILCGNKKDL DADREVTFLE ASRFAQENEL
160 170 180 190 200
MFLETSALTG ENVEEAFVQC ARKILNKIES GELDPERMGS GIQYGDAALR
210
QLRSPRRAQA PNAQECGC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WYT5 | A0A087WYT5_HUMAN | Ras-related protein Rab-4A | RAB4A | 113 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 162 | N → D in AAA60244 (PubMed:2501306).Curated | 1 | |
Sequence conflicti | 208 | A → T in AAA60244 (PubMed:2501306).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28211 mRNA Translation: AAA60244.1 Frameshift. AY585832 mRNA Translation: AAT91347.1 AF498934 mRNA Translation: AAM21082.1 AK313807 mRNA Translation: BAG36543.1 AL162595 Genomic DNA No translation available. AL117350 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW69890.1 BC002438 mRNA Translation: AAH02438.1 BC004309 mRNA Translation: AAH04309.1 |
CCDSi | CCDS31050.1 |
PIRi | E34323 |
RefSeqi | NP_001258927.1, NM_001271998.1 NP_004569.2, NM_004578.3 |
Genome annotation databases
Ensembli | ENST00000366690; ENSP00000355651; ENSG00000168118 |
GeneIDi | 5867 |
KEGGi | hsa:5867 |
MANE-Selecti | ENST00000366690.5; ENSP00000355651.4; NM_004578.4; NP_004569.2 |
UCSCi | uc001hth.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28211 mRNA Translation: AAA60244.1 Frameshift. AY585832 mRNA Translation: AAT91347.1 AF498934 mRNA Translation: AAM21082.1 AK313807 mRNA Translation: BAG36543.1 AL162595 Genomic DNA No translation available. AL117350 Genomic DNA No translation available. CH471098 Genomic DNA Translation: EAW69890.1 BC002438 mRNA Translation: AAH02438.1 BC004309 mRNA Translation: AAH04309.1 |
CCDSi | CCDS31050.1 |
PIRi | E34323 |
RefSeqi | NP_001258927.1, NM_001271998.1 NP_004569.2, NM_004578.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YU9 | X-ray | 2.07 | A | 7-177 | [»] | |
1Z0K | X-ray | 1.92 | A/C | 7-177 | [»] | |
2BMD | X-ray | 1.80 | A | 6-189 | [»] | |
2BME | X-ray | 1.57 | A/B/C/D | 6-189 | [»] | |
SMRi | P20338 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111805, 379 interactors |
DIPi | DIP-372N |
IntActi | P20338, 31 interactors |
MINTi | P20338 |
STRINGi | 9606.ENSP00000355651 |
Protein family/group databases
MoonDBi | P20338, Predicted |
PTM databases
iPTMneti | P20338 |
PhosphoSitePlusi | P20338 |
Genetic variation databases
BioMutai | RAB4A |
DMDMi | 460018296 |
Proteomic databases
EPDi | P20338 |
jPOSTi | P20338 |
MassIVEi | P20338 |
MaxQBi | P20338 |
PaxDbi | P20338 |
PeptideAtlasi | P20338 |
PRIDEi | P20338 |
ProteomicsDBi | 53750 |
Protocols and materials databases
Antibodypediai | 34673, 341 antibodies from 35 providers |
DNASUi | 5867 |
Genome annotation databases
Ensembli | ENST00000366690; ENSP00000355651; ENSG00000168118 |
GeneIDi | 5867 |
KEGGi | hsa:5867 |
MANE-Selecti | ENST00000366690.5; ENSP00000355651.4; NM_004578.4; NP_004569.2 |
UCSCi | uc001hth.5, human |
Organism-specific databases
CTDi | 5867 |
DisGeNETi | 5867 |
GeneCardsi | RAB4A |
HGNCi | HGNC:9781, RAB4A |
HPAi | ENSG00000168118, Low tissue specificity |
MIMi | 179511, gene |
neXtProti | NX_P20338 |
OpenTargetsi | ENSG00000168118 |
PharmGKBi | PA34141 |
VEuPathDBi | HostDB:ENSG00000168118 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0086, Eukaryota |
GeneTreei | ENSGT00940000157464 |
HOGENOMi | CLU_041217_23_1_1 |
InParanoidi | P20338 |
OMAi | STQECGC |
OrthoDBi | 1146851at2759 |
PhylomeDBi | P20338 |
TreeFami | TF300032 |
Enzyme and pathway databases
PathwayCommonsi | P20338 |
Reactomei | R-HSA-1445148, Translocation of SLC2A4 (GLUT4) to the plasma membrane R-HSA-1660499, Synthesis of PIPs at the plasma membrane R-HSA-8854214, TBC/RABGAPs R-HSA-8873719, RAB geranylgeranylation R-HSA-8875656, MET receptor recycling |
SignaLinki | P20338 |
SIGNORi | P20338 |
Miscellaneous databases
BioGRID-ORCSi | 5867, 44 hits in 1047 CRISPR screens |
ChiTaRSi | RAB4A, human |
EvolutionaryTracei | P20338 |
GeneWikii | RAB4A |
GenomeRNAii | 5867 |
Pharosi | P20338, Tbio |
PROi | PR:P20338 |
RNActi | P20338, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000168118, Expressed in vagina and 251 other tissues |
ExpressionAtlasi | P20338, baseline and differential |
Genevisiblei | P20338, HS |
Family and domain databases
CDDi | cd04113, Rab4, 1 hit |
Gene3Di | 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR041819, Rab4 IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SMARTi | View protein in SMART SM00174, RHO, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB4A_HUMAN | |
Accessioni | P20338Primary (citable) accession number: P20338 Secondary accession number(s): Q5T7P7, Q9BQ44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | March 6, 2013 | |
Last modified: | February 23, 2022 | |
This is version 220 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families