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UniProtKB - P20309 (ACM3_HUMAN)

Entry version 201 (07 Oct 2020)
Sequence version 1 (01 Feb 1991)
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Protein

Muscarinic acetylcholine receptor M3

Gene

CHRM3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		P20309

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-390648, Muscarinic acetylcholine receptors
R-HSA-399997, Acetylcholine regulates insulin secretion
R-HSA-416476, G alpha (q) signalling events

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		P20309

SIGNOR Signaling Network Open Resource

More...SIGNORi		P20309

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Muscarinic acetylcholine receptor M3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:CHRM3
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		HostDB:ENSG00000133019.11

Human Gene Nomenclature Database

More...HGNCi		HGNC:1952, CHRM3

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		118494, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_P20309

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 67ExtracellularBy similarityAdd BLAST67
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei68 – 91Helical; Name=1By similarityAdd BLAST24
Topological domaini92 – 104CytoplasmicBy similarityAdd BLAST13
Transmembranei105 – 130Helical; Name=2By similarityAdd BLAST26
Topological domaini131 – 142ExtracellularBy similarityAdd BLAST12
Transmembranei143 – 164Helical; Name=3By similarityAdd BLAST22
Topological domaini165 – 184CytoplasmicBy similarityAdd BLAST20
Transmembranei185 – 206Helical; Name=4By similarityAdd BLAST22
Topological domaini207 – 229ExtracellularBy similarityAdd BLAST23
Transmembranei230 – 252Helical; Name=5By similarityAdd BLAST23
Topological domaini253 – 491CytoplasmicBy similarityAdd BLAST239
Transmembranei492 – 514Helical; Name=6By similarityAdd BLAST23
Topological domaini515 – 526ExtracellularBy similarityAdd BLAST12
Transmembranei527 – 546Helical; Name=7By similarityAdd BLAST20
Topological domaini547 – 590CytoplasmicBy similarityAdd BLAST44

Keywords - Cellular componenti

Cell junction, Cell membrane, Endoplasmic reticulum, Membrane, Postsynaptic cell membrane, Synapse

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

Prune belly syndrome (PBS)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities.
Related information in OMIM

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi276E → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi276E → D: Loss of basolateral sorting. No effect on basolateral sorting; when associated with L-280 and L-281. 1 Publication1
Mutagenesisi280F → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi280F → L: No effect on basolateral sorting. 1 Publication1
Mutagenesisi281V → A: Loss of basolateral sorting. 1 Publication1
Mutagenesisi281V → L: No effect on basolateral sorting. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		1131

MalaCards human disease database

More...MalaCardsi		CHRM3
MIMi100100, phenotype

Open Targets

More...OpenTargetsi		ENSG00000133019

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		2970, Prune belly syndrome

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA112

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		P20309, Tclin

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL245

Drug and drug target database

More...DrugBanki		DB03128, Acetylcholine
DB08897, Aclidinium
DB05752, ALKS 27
DB00321, Amitriptyline
DB00543, Amoxapine
DB00517, Anisotropine methylbromide
DB04365, Arecoline
DB01238, Aripiprazole
DB14185, Aripiprazole lauroxil
DB00572, Atropine
DB00767, Benzquinamide
DB01019, Bethanechol
DB00835, Brompheniramine
DB09300, Butylscopolamine
DB00411, Carbamoylcholine
DB00185, Cevimeline
DB00477, Chlorpromazine
DB01239, Chlorprothixene
DB00568, Cinnarizine
DB00363, Clozapine
DB00785, Cryptenamine
DB00434, Cyproheptadine
DB00496, Darifenacin
DB01151, Desipramine
DB13720, Diphemanil
DB01231, Diphenidol
DB00280, Disopyramide
DB09167, Dosulepin
DB01142, Doxepin
DB09194, Etoperidone
DB06702, Fesoterodine
DB00986, Glycopyrronium
DB00502, Haloperidol
DB06787, Hexocyclium
DB11181, Homatropine
DB00725, Homatropine methylbromide
DB00424, Hyoscyamine
DB09262, Imidafenacin
DB00458, Imipramine
DB00332, Ipratropium
DB01625, Isopropamide
DB01221, Ketamine
DB00408, Loxapine
DB00934, Maprotiline
DB04843, Mepenzolate
DB00454, Meperidine
DB06709, Methacholine
DB01403, Methotrimeprazine
DB00462, Methscopolamine bromide
DB00340, Metixene
DB01226, Mivacurium
DB00622, Nicardipine
DB00540, Nortriptyline
DB00334, Olanzapine
DB01062, Oxybutynin
DB00383, Oxyphencyclimine
DB01337, Pancuronium
DB00715, Paroxetine
DB01085, Pilocarpine
DB01338, Pipecuronium
DB06153, Pizotifen
DB00387, Procyclidine
DB00420, Promazine
DB01069, Promethazine
DB00777, Propiomazine
DB12278, Propiverine
DB01224, Quetiapine
DB11855, Revefenacin
DB13581, Rociverine
DB00747, Scopolamine
DB01591, Solifenacin
DB00202, Succinylcholine
DB00342, Terfenadine
DB00599, Thiopental
DB11235, Thonzylamine
DB01409, Tiotropium
DB01036, Tolterodine
DB00193, Tramadol
DB00505, Tridihexethyl
DB00376, Trihexyphenidyl
DB09089, Trimebutine
DB00726, Trimipramine
DB00809, Tropicamide
DB09076, Umeclidinium
DB00246, Ziprasidone

DrugCentral

More...DrugCentrali		P20309

IUPHAR/BPS Guide to PHARMACOLOGY

More...GuidetoPHARMACOLOGYi		15

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		CHRM3

Domain mapping of disease mutations (DMDM)

More...DMDMi		113125

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000690291 – 590Muscarinic acetylcholine receptor M3Add BLAST590

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi6N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi15N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi41N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
<p>This subsection of the PTM / Processing":/help/ptm_processing_section section describes the positions of cysteine residues participating in disulfide bonds.<p><a href='/help/disulfid' target='_top'>More...</a></p>Disulfide bondi141 ↔ 221PROSITE-ProRule annotation
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei385PhosphoserineBy similarity1
Disulfide bondi517 ↔ 520PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P20309

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		P20309

MaxQB - The MaxQuant DataBase

More...MaxQBi		P20309

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P20309

PeptideAtlas

More...PeptideAtlasi		P20309

PRoteomics IDEntifications database

More...PRIDEi		P20309

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		53745

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		P20309, 5 sites

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		P20309

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		P20309

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000133019, Expressed in endothelial cell and 179 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		P20309, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		P20309, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000133019, Tissue enhanced (retina)

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer; the dimers can form tetramers (PubMed:23521066).

Interacts with NALCN (By similarity).

Interacts with TMEM147 (PubMed:21056967).

By similarity2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		107553, 60 interactors

Database of interacting proteins

More...DIPi		DIP-44291N

Protein interaction database and analysis system

More...IntActi		P20309, 65 interactors

Molecular INTeraction database

More...MINTi		P20309

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000255380

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P20309

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		P20309, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1590
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P20309

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P20309

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni148 – 152Agonist bindingBy similarity5
Regioni507 – 530Agonist bindingBy similarityAdd BLAST24

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi275 – 281Basolateral sorting signal7

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG4220, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000160084

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_009579_11_2_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P20309

KEGG Orthology (KO)

More...KOi		K04131

Identification of Orthologs from Complete Genome Data

More...OMAi		VIFHKRI

Database of Orthologous Groups

More...OrthoDBi		1245472at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		P20309

TreeFam database of animal gene trees

More...TreeFami		TF320495

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR000276, GPCR_Rhodpsn
IPR017452, GPCR_Rhodpsn_7TM
IPR001183, Musac_Ach_M3_rcpt
IPR000995, Musac_Ach_rcpt

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00001, 7tm_1, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00237, GPCRRHODOPSN
PR00243, MUSCARINICR
PR00540, MUSCRINICM3R

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM01381, 7TM_GPCR_Srsx, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00237, G_PROTEIN_RECEP_F1_1, 1 hit
PS50262, G_PROTEIN_RECEP_F1_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P20309-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLHNNSTTS PLFPNISSSW IHSPSDAGLP PGTVTHFGSY NVSRAAGNFS 
        60         70         80         90        100
SPDGTTDDPL GGHTVWQVVF IAFLTGILAL VTIIGNILVI VSFKVNKQLK 
       110        120        130        140        150
TVNNYFLLSL ACADLIIGVI SMNLFTTYII MNRWALGNLA CDLWLAIDYV 
       160        170        180        190        200
ASNASVMNLL VISFDRYFSI TRPLTYRAKR TTKRAGVMIG LAWVISFVLW 
       210        220        230        240        250
APAILFWQYF VGKRTVPPGE CFIQFLSEPT ITFGTAIAAF YMPVTIMTIL 
       260        270        280        290        300
YWRIYKETEK RTKELAGLQA SGTEAETENF VHPTGSSRSC SSYELQQQSM 
       310        320        330        340        350
KRSNRRKYGR CHFWFTTKSW KPSSEQMDQD HSSSDSWNNN DAAASLENSA 
       360        370        380        390        400
SSDEEDIGSE TRAIYSIVLK LPGHSTILNS TKLPSSDNLQ VPEEELGMVD 
       410        420        430        440        450
LERKADKLQA QKSVDDGGSF PKSFSKLPIQ LESAVDTAKT SDVNSSVGKS 
       460        470        480        490        500
TATLPLSFKE ATLAKRFALK TRSQITKRKR MSLVKEKKAA QTLSAILLAF 
       510        520        530        540        550
IITWTPYNIM VLVNTFCDSC IPKTFWNLGY WLCYINSTVN PVCYALCNKT 
       560        570        580        590
FRTTFKMLLL CQCDKKKRRK QQYQQRQSVI FHKRAPEQAL
Length:590
Mass (Da):66,128
Last modified:February 1, 1991 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5CB473C57B9526E9
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AN12B1AN12_HUMAN
Muscarinic acetylcholine receptor M...
CHRM3
109Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti382 – 384KLP → RLS in AAG30036 (PubMed:11238933).Curated3

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_03346165V → I. Corresponds to variant dbSNP:rs2067481Ensembl.1
Natural variantiVAR_049368431L → P. Corresponds to variant dbSNP:rs16839102EnsemblClinVar.1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X15266 Genomic DNA Translation: CAA33337.1
U29589 Genomic DNA Translation: AAA70337.1
AB041395 Genomic DNA Translation: BAA94480.1
AF498917 mRNA Translation: AAM18940.1
AL356361 Genomic DNA No translation available.
BC096844 mRNA Translation: AAH96844.1
BC121026 mRNA Translation: AAI21027.1
AF279779 mRNA Translation: AAG30036.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS1616.1

Protein sequence database of the Protein Information Resource

More...PIRi		S10128

NCBI Reference Sequences

More...RefSeqi		NP_000731.1, NM_000740.3
NP_001334645.1, NM_001347716.1
XP_005273089.1, XM_005273032.3
XP_011542343.1, XM_011544041.2
XP_011542345.1, XM_011544043.2
XP_011542346.1, XM_011544044.2
XP_011542348.1, XM_011544046.2
XP_011542349.1, XM_011544047.2
XP_016855641.1, XM_017000152.1
XP_016855642.1, XM_017000153.1
XP_016855643.1, XM_017000154.1
XP_016855644.1, XM_017000155.1
XP_016855645.1, XM_017000156.1
XP_016855646.1, XM_017000157.1
XP_016855647.1, XM_017000158.1
XP_016855648.1, XM_017000159.1
XP_016855649.1, XM_017000160.1
XP_016855650.1, XM_017000161.1
XP_016855651.1, XM_017000162.1
XP_016855652.1, XM_017000163.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000255380; ENSP00000255380; ENSG00000133019
ENST00000615928; ENSP00000482377; ENSG00000133019

Database of genes from NCBI RefSeq genomes

More...GeneIDi		1131

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:1131

UCSC genome browser

More...UCSCi		uc001hyp.4, human

Keywords - Coding sequence diversityi

Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15266 Genomic DNA Translation: CAA33337.1
U29589 Genomic DNA Translation: AAA70337.1
AB041395 Genomic DNA Translation: BAA94480.1
AF498917 mRNA Translation: AAM18940.1
AL356361 Genomic DNA No translation available.
BC096844 mRNA Translation: AAH96844.1
BC121026 mRNA Translation: AAI21027.1
AF279779 mRNA Translation: AAG30036.1
CCDSiCCDS1616.1
PIRiS10128
RefSeqiNP_000731.1, NM_000740.3
NP_001334645.1, NM_001347716.1
XP_005273089.1, XM_005273032.3
XP_011542343.1, XM_011544041.2
XP_011542345.1, XM_011544043.2
XP_011542346.1, XM_011544044.2
XP_011542348.1, XM_011544046.2
XP_011542349.1, XM_011544047.2
XP_016855641.1, XM_017000152.1
XP_016855642.1, XM_017000153.1
XP_016855643.1, XM_017000154.1
XP_016855644.1, XM_017000155.1
XP_016855645.1, XM_017000156.1
XP_016855646.1, XM_017000157.1
XP_016855647.1, XM_017000158.1
XP_016855648.1, XM_017000159.1
XP_016855649.1, XM_017000160.1
XP_016855650.1, XM_017000161.1
XP_016855651.1, XM_017000162.1
XP_016855652.1, XM_017000163.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CSANMR-A271-289[»]
SMRiP20309
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi107553, 60 interactors
DIPiDIP-44291N
IntActiP20309, 65 interactors
MINTiP20309
STRINGi9606.ENSP00000255380

Chemistry databases

BindingDBiP20309
ChEMBLiCHEMBL245
DrugBankiDB03128, Acetylcholine
DB08897, Aclidinium
DB05752, ALKS 27
DB00321, Amitriptyline
DB00543, Amoxapine
DB00517, Anisotropine methylbromide
DB04365, Arecoline
DB01238, Aripiprazole
DB14185, Aripiprazole lauroxil
DB00572, Atropine
DB00767, Benzquinamide
DB01019, Bethanechol
DB00835, Brompheniramine
DB09300, Butylscopolamine
DB00411, Carbamoylcholine
DB00185, Cevimeline
DB00477, Chlorpromazine
DB01239, Chlorprothixene
DB00568, Cinnarizine
DB00363, Clozapine
DB00785, Cryptenamine
DB00434, Cyproheptadine
DB00496, Darifenacin
DB01151, Desipramine
DB13720, Diphemanil
DB01231, Diphenidol
DB00280, Disopyramide
DB09167, Dosulepin
DB01142, Doxepin
DB09194, Etoperidone
DB06702, Fesoterodine
DB00986, Glycopyrronium
DB00502, Haloperidol
DB06787, Hexocyclium
DB11181, Homatropine
DB00725, Homatropine methylbromide
DB00424, Hyoscyamine
DB09262, Imidafenacin
DB00458, Imipramine
DB00332, Ipratropium
DB01625, Isopropamide
DB01221, Ketamine
DB00408, Loxapine
DB00934, Maprotiline
DB04843, Mepenzolate
DB00454, Meperidine
DB06709, Methacholine
DB01403, Methotrimeprazine
DB00462, Methscopolamine bromide
DB00340, Metixene
DB01226, Mivacurium
DB00622, Nicardipine
DB00540, Nortriptyline
DB00334, Olanzapine
DB01062, Oxybutynin
DB00383, Oxyphencyclimine
DB01337, Pancuronium
DB00715, Paroxetine
DB01085, Pilocarpine
DB01338, Pipecuronium
DB06153, Pizotifen
DB00387, Procyclidine
DB00420, Promazine
DB01069, Promethazine
DB00777, Propiomazine
DB12278, Propiverine
DB01224, Quetiapine
DB11855, Revefenacin
DB13581, Rociverine
DB00747, Scopolamine
DB01591, Solifenacin
DB00202, Succinylcholine
DB00342, Terfenadine
DB00599, Thiopental
DB11235, Thonzylamine
DB01409, Tiotropium
DB01036, Tolterodine
DB00193, Tramadol
DB00505, Tridihexethyl
DB00376, Trihexyphenidyl
DB09089, Trimebutine
DB00726, Trimipramine
DB00809, Tropicamide
DB09076, Umeclidinium
DB00246, Ziprasidone
DrugCentraliP20309
GuidetoPHARMACOLOGYi15

Protein family/group databases

Information system for G protein-coupled receptors (GPCRs)

More...GPCRDBi		Search...

PTM databases

GlyGeniP20309, 5 sites
iPTMnetiP20309
PhosphoSitePlusiP20309

Polymorphism and mutation databases

BioMutaiCHRM3
DMDMi113125

Proteomic databases

jPOSTiP20309
MassIVEiP20309
MaxQBiP20309
PaxDbiP20309
PeptideAtlasiP20309
PRIDEiP20309
ProteomicsDBi53745

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		4169, 362 antibodies

The DNASU plasmid repository

More...DNASUi		1131

Genome annotation databases

EnsembliENST00000255380; ENSP00000255380; ENSG00000133019
ENST00000615928; ENSP00000482377; ENSG00000133019
GeneIDi1131
KEGGihsa:1131
UCSCiuc001hyp.4, human

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		1131
DisGeNETi1131
EuPathDBiHostDB:ENSG00000133019.11

GeneCards: human genes, protein and diseases

More...GeneCardsi		CHRM3
HGNCiHGNC:1952, CHRM3
HPAiENSG00000133019, Tissue enhanced (retina)
MalaCardsiCHRM3
MIMi100100, phenotype
118494, gene
neXtProtiNX_P20309
OpenTargetsiENSG00000133019
Orphaneti2970, Prune belly syndrome
PharmGKBiPA112

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG4220, Eukaryota
GeneTreeiENSGT00940000160084
HOGENOMiCLU_009579_11_2_1
InParanoidiP20309
KOiK04131
OMAiVIFHKRI
OrthoDBi1245472at2759
PhylomeDBiP20309
TreeFamiTF320495

Enzyme and pathway databases

PathwayCommonsiP20309
ReactomeiR-HSA-390648, Muscarinic acetylcholine receptors
R-HSA-399997, Acetylcholine regulates insulin secretion
R-HSA-416476, G alpha (q) signalling events
SignaLinkiP20309
SIGNORiP20309

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		1131, 3 hits in 870 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		CHRM3, human
EvolutionaryTraceiP20309

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		Muscarinic_acetylcholine_receptor_M3

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		1131
PharosiP20309, Tclin

Protein Ontology

More...PROi		PR:P20309
RNActiP20309, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000133019, Expressed in endothelial cell and 179 other tissues
ExpressionAtlasiP20309, baseline and differential
GenevisibleiP20309, HS

Family and domain databases

InterProiView protein in InterPro
IPR000276, GPCR_Rhodpsn
IPR017452, GPCR_Rhodpsn_7TM
IPR001183, Musac_Ach_M3_rcpt
IPR000995, Musac_Ach_rcpt
PfamiView protein in Pfam
PF00001, 7tm_1, 1 hit
PRINTSiPR00237, GPCRRHODOPSN
PR00243, MUSCARINICR
PR00540, MUSCRINICM3R
SMARTiView protein in SMART
SM01381, 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237, G_PROTEIN_RECEP_F1_1, 1 hit
PS50262, G_PROTEIN_RECEP_F1_2, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiACM3_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P20309
Secondary accession number(s): Q0VAJ8
, Q4QRI3, Q5VXY2, Q9HB60
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: October 7, 2020
This is version 201 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families
  6. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  7. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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