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Protein

Arachidonate 5-lipoxygenase-activating protein

Gene

Alox5ap

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes (By similarity).By similarity

GO - Molecular functioni

  • arachidonic acid binding Source: UniProtKB
  • enzyme activator activity Source: InterPro
  • enzyme binding Source: RGD
  • glutathione peroxidase activity Source: GO_Central
  • glutathione transferase activity Source: GO_Central
  • leukotriene-C4 synthase activity Source: GO_Central
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

GO - Biological processi

Keywordsi

Biological processLeukotriene biosynthesis

Enzyme and pathway databases

ReactomeiR-RNO-2142688 Synthesis of 5-eicosatetraenoic acids
R-RNO-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-RNO-2142700 Synthesis of Lipoxins (LX)

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase-activating protein
Alternative name(s):
FLAP
MK-886-binding protein
Gene namesi
Name:Alox5ap
Synonyms:Flap
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi2097 Alox5ap

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8LumenalBy similarity8
Transmembranei9 – 30HelicalBy similarityAdd BLAST22
Topological domaini31 – 52CytoplasmicBy similarityAdd BLAST22
Transmembranei53 – 77HelicalBy similarityAdd BLAST25
Topological domaini78 – 80LumenalBy similarity3
Transmembranei81 – 102HelicalBy similarityAdd BLAST22
Topological domaini103 – 107CytoplasmicBy similarity5
Intramembranei108 – 115By similarity8
Transmembranei116 – 128HelicalBy similarityAdd BLAST13
Topological domaini129 – 161LumenalBy similarityAdd BLAST33

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1921661

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002177561 – 161Arachidonate 5-lipoxygenase-activating proteinAdd BLAST161

Proteomic databases

PaxDbiP20291
PRIDEiP20291

PTM databases

PhosphoSitePlusiP20291

Expressioni

Gene expression databases

BgeeiENSRNOG00000000907 Expressed in 10 organ(s), highest expression level in lung
GenevisibleiP20291 RN

Interactioni

Subunit structurei

Homotrimer. Interacts with LTC4S and ALOX5 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48660N
IntActiP20291, 1 interactor
STRINGi10116.ENSRNOP00000001207

Chemistry databases

BindingDBiP20291

Structurei

3D structure databases

ProteinModelPortaliP20291
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 27Inhibitor bindingBy similarity8
Regioni112 – 123Inhibitor bindingBy similarityAdd BLAST12

Domaini

The C-terminal part after residue 140 is mostly disordered.By similarity

Sequence similaritiesi

Belongs to the MAPEG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHE0 Eukaryota
ENOG4111T4I LUCA
GeneTreeiENSGT00430000030964
HOGENOMiHOG000116372
HOVERGENiHBG107295
InParanoidiP20291
KOiK20735
OMAiLAVMWCA
OrthoDBiEOG091G12NW
PhylomeDBiP20291
TreeFamiTF105328

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR001446 5_LipOase_AP
IPR018295 FLAP/GST2/LTC4S_CS
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
PRINTSiPR00488 5LPOXGNASEAP
SUPFAMiSSF161084 SSF161084, 1 hit
PROSITEiView protein in PROSITE
PS01297 FLAP_GST2_LTC4S, 1 hit

Sequencei

Sequence statusi: Complete.

P20291-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDQEAVGNVV LLAIVTLISV VQNAFFAHKV ELESKAQSGR SFQRTGTLAF
60 70 80 90 100
ERVYTANQNC VDAYPTFLVV LWTAGLLCSQ VPAAFAGLMY LFVRQKYFVG
110 120 130 140 150
YLGERTQSTP GYIFGKRIIL FLFLMSLAGI LNHYLIFFFG SDFENYIRTI
160
TTTISPLLLI P
Length:161
Mass (Da):18,070
Last modified:May 1, 2007 - v2
Checksum:i9CCD6F8B3AA3B87E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti32L → I AA sequence (PubMed:2300172).Curated1
Sequence conflicti127L → I AA sequence (PubMed:2300172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52196 mRNA Translation: CAA36442.1
BC086593 mRNA Translation: AAH86593.1
PIRiS08206
RefSeqiNP_058956.1, NM_017260.2
UniGeneiRn.18399

Genome annotation databases

EnsembliENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907
GeneIDi29624
KEGGirno:29624
UCSCiRGD:2097 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52196 mRNA Translation: CAA36442.1
BC086593 mRNA Translation: AAH86593.1
PIRiS08206
RefSeqiNP_058956.1, NM_017260.2
UniGeneiRn.18399

3D structure databases

ProteinModelPortaliP20291
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48660N
IntActiP20291, 1 interactor
STRINGi10116.ENSRNOP00000001207

Chemistry databases

BindingDBiP20291
ChEMBLiCHEMBL1921661

PTM databases

PhosphoSitePlusiP20291

Proteomic databases

PaxDbiP20291
PRIDEiP20291

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001207; ENSRNOP00000001207; ENSRNOG00000000907
GeneIDi29624
KEGGirno:29624
UCSCiRGD:2097 rat

Organism-specific databases

CTDi241
RGDi2097 Alox5ap

Phylogenomic databases

eggNOGiENOG410IHE0 Eukaryota
ENOG4111T4I LUCA
GeneTreeiENSGT00430000030964
HOGENOMiHOG000116372
HOVERGENiHBG107295
InParanoidiP20291
KOiK20735
OMAiLAVMWCA
OrthoDBiEOG091G12NW
PhylomeDBiP20291
TreeFamiTF105328

Enzyme and pathway databases

ReactomeiR-RNO-2142688 Synthesis of 5-eicosatetraenoic acids
R-RNO-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-RNO-2142700 Synthesis of Lipoxins (LX)

Miscellaneous databases

PROiPR:P20291

Gene expression databases

BgeeiENSRNOG00000000907 Expressed in 10 organ(s), highest expression level in lung
GenevisibleiP20291 RN

Family and domain databases

Gene3Di1.20.120.550, 1 hit
InterProiView protein in InterPro
IPR001446 5_LipOase_AP
IPR018295 FLAP/GST2/LTC4S_CS
IPR023352 MAPEG-like_dom_sf
IPR001129 Membr-assoc_MAPEG
PfamiView protein in Pfam
PF01124 MAPEG, 1 hit
PRINTSiPR00488 5LPOXGNASEAP
SUPFAMiSSF161084 SSF161084, 1 hit
PROSITEiView protein in PROSITE
PS01297 FLAP_GST2_LTC4S, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAL5AP_RAT
AccessioniPrimary (citable) accession number: P20291
Secondary accession number(s): Q5RJL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 128 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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