Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription factor BTF3

Gene

BTF3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.1 Publication

GO - Molecular functioni

GO - Biological processi

  • in utero embryonic development Source: GO_Central
  • protein transport Source: UniProtKB-KW
  • transcription by RNA polymerase II Source: ProtInc

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

SIGNORiP20290

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor BTF3
Alternative name(s):
Nascent polypeptide-associated complex subunit beta
Short name:
NAC-beta
RNA polymerase B transcription factor 3
Gene namesi
Name:BTF3
Synonyms:NACB
ORF Names:OK/SW-cl.8
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000145741.15
HGNCiHGNC:1125 BTF3
MIMi602542 gene
neXtProtiNX_P20290

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi689
OpenTargetsiENSG00000145741
PharmGKBiPA25445

Polymorphism and mutation databases

BioMutaiBTF3
DMDMi115143

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135481 – 206Transcription factor BTF3Add BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19Omega-N-methylarginineCombined sources1
Modified residuei30PhosphoserineCombined sources1
Modified residuei46N6-methyllysineCombined sources1
Modified residuei54N6-methyllysineCombined sources1
Modified residuei160PhosphothreonineCombined sources1
Modified residuei173PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP20290
MaxQBiP20290
PaxDbiP20290
PeptideAtlasiP20290
PRIDEiP20290
ProteomicsDBi53742
53743 [P20290-2]
TopDownProteomicsiP20290-1 [P20290-1]
P20290-2 [P20290-2]

PTM databases

iPTMnetiP20290
PhosphoSitePlusiP20290
SwissPalmiP20290

Expressioni

Gene expression databases

BgeeiENSG00000145741 Expressed in 233 organ(s), highest expression level in thoracic mammary gland
CleanExiHS_BTF3
ExpressionAtlasiP20290 baseline and differential
GenevisibleiP20290 HS

Organism-specific databases

HPAiCAB013007
HPA056420

Interactioni

Subunit structurei

Part of the nascent polypeptide-associated complex (NAC), which is a heterodimer of NACA and BTF3 (via NAC-A/B domains). NAC associates with ribosomes through the BTF3/NACB subunit. Both subunits can contact nascent polypeptide chains.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi107154, 97 interactors
IntActiP20290, 38 interactors
MINTiP20290
STRINGi9606.ENSP00000369965

Structurei

Secondary structure

1206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20290
SMRiP20290
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20290

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini82 – 147NAC-A/BPROSITE-ProRule annotationAdd BLAST66

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi185 – 188Poly-Asp4

Sequence similaritiesi

Belongs to the NAC-beta family.Curated

Phylogenomic databases

eggNOGiKOG2240 Eukaryota
ENOG4111JBG LUCA
GeneTreeiENSGT00390000006283
HOGENOMiHOG000261245
HOVERGENiHBG004906
InParanoidiP20290
KOiK01527
OMAiMKEAIMN
OrthoDBiEOG091G0TSG
PhylomeDBiP20290
TreeFamiTF317546

Family and domain databases

Gene3Di2.20.70.30, 1 hit
InterProiView protein in InterPro
IPR038187 NAC_A/B_dom_sf
IPR002715 Nas_poly-pep-assoc_cplx_dom
PfamiView protein in Pfam
PF01849 NAC, 1 hit
SMARTiView protein in SMART
SM01407 NAC, 1 hit
PROSITEiView protein in PROSITE
PS51151 NAC_AB, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P20290-1) [UniParc]FASTAAdd to basket
Also known as: BTF3a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRRTGAPAQA DSRGRGRARG GCPGGEATLS QPPPRGGTRG QEPQMKETIM
60 70 80 90 100
NQEKLAKLQA QVRIGGKGTA RRKKKVVHRT ATADDKKLQF SLKKLGVNNI
110 120 130 140 150
SGIEEVNMFT NQGTVIHFNN PKVQASLAAN TFTITGHAET KQLTEMLPSI
160 170 180 190 200
LNQLGADSLT SLRRLAEALP KQSVDGKAPL ATGEDDDDEV PDLVENFDEA

SKNEAN
Length:206
Mass (Da):22,168
Last modified:February 1, 1991 - v1
Checksum:i9653AC480EAF64C6
GO
Isoform 2 (identifier: P20290-2) [UniParc]FASTAAdd to basket
Also known as: BTF3b

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.

Show »
Length:162
Mass (Da):17,699
Checksum:i5062DE8858CEE979
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RDG3D6RDG3_HUMAN
Transcription factor BTF3
BTF3
109Annotation score:
H0Y9Y1H0Y9Y1_HUMAN
Transcription factor BTF3
BTF3
86Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41Q → E in AAA58398 (PubMed:1386332).Curated1
Sequence conflicti68 – 105Missing in AAA58398 (PubMed:1386332).CuratedAdd BLAST38
Sequence conflicti192 – 196DLVEN → GG (PubMed:1386332).Curated5
Sequence conflicti198D → Q (PubMed:1386332).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0135871 – 44Missing in isoform 2. 6 PublicationsAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53280 mRNA Translation: CAA37375.1
X53281 mRNA Translation: CAA37376.1
M90357, M90352 Genomic DNA Translation: AAA58398.1
X74070 mRNA Translation: CAA52200.1
AB062126 mRNA Translation: BAB93458.1
BT007120 mRNA Translation: AAP35784.1
AK291125 mRNA Translation: BAF83814.1
CH471084 Genomic DNA Translation: EAW95727.1
BC008062 mRNA Translation: AAH08062.1
CCDSiCCDS34185.1 [P20290-1]
CCDS4019.1 [P20290-2]
PIRiJC1235
RefSeqiNP_001032726.1, NM_001037637.1 [P20290-1]
NP_001198.2, NM_001207.4 [P20290-2]
UniGeneiHs.591768

Genome annotation databases

EnsembliENST00000335895; ENSP00000338516; ENSG00000145741 [P20290-2]
ENST00000380591; ENSP00000369965; ENSG00000145741 [P20290-1]
GeneIDi689
KEGGihsa:689
UCSCiuc003kcq.2 human [P20290-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53280 mRNA Translation: CAA37375.1
X53281 mRNA Translation: CAA37376.1
M90357, M90352 Genomic DNA Translation: AAA58398.1
X74070 mRNA Translation: CAA52200.1
AB062126 mRNA Translation: BAB93458.1
BT007120 mRNA Translation: AAP35784.1
AK291125 mRNA Translation: BAF83814.1
CH471084 Genomic DNA Translation: EAW95727.1
BC008062 mRNA Translation: AAH08062.1
CCDSiCCDS34185.1 [P20290-1]
CCDS4019.1 [P20290-2]
PIRiJC1235
RefSeqiNP_001032726.1, NM_001037637.1 [P20290-1]
NP_001198.2, NM_001207.4 [P20290-2]
UniGeneiHs.591768

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LKXX-ray2.50A97-162[»]
3MCBX-ray1.90B97-154[»]
ProteinModelPortaliP20290
SMRiP20290
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107154, 97 interactors
IntActiP20290, 38 interactors
MINTiP20290
STRINGi9606.ENSP00000369965

PTM databases

iPTMnetiP20290
PhosphoSitePlusiP20290
SwissPalmiP20290

Polymorphism and mutation databases

BioMutaiBTF3
DMDMi115143

Proteomic databases

EPDiP20290
MaxQBiP20290
PaxDbiP20290
PeptideAtlasiP20290
PRIDEiP20290
ProteomicsDBi53742
53743 [P20290-2]
TopDownProteomicsiP20290-1 [P20290-1]
P20290-2 [P20290-2]

Protocols and materials databases

DNASUi689
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000335895; ENSP00000338516; ENSG00000145741 [P20290-2]
ENST00000380591; ENSP00000369965; ENSG00000145741 [P20290-1]
GeneIDi689
KEGGihsa:689
UCSCiuc003kcq.2 human [P20290-1]

Organism-specific databases

CTDi689
DisGeNETi689
EuPathDBiHostDB:ENSG00000145741.15
GeneCardsiBTF3
HGNCiHGNC:1125 BTF3
HPAiCAB013007
HPA056420
MIMi602542 gene
neXtProtiNX_P20290
OpenTargetsiENSG00000145741
PharmGKBiPA25445
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2240 Eukaryota
ENOG4111JBG LUCA
GeneTreeiENSGT00390000006283
HOGENOMiHOG000261245
HOVERGENiHBG004906
InParanoidiP20290
KOiK01527
OMAiMKEAIMN
OrthoDBiEOG091G0TSG
PhylomeDBiP20290
TreeFamiTF317546

Enzyme and pathway databases

SIGNORiP20290

Miscellaneous databases

ChiTaRSiBTF3 human
EvolutionaryTraceiP20290
GeneWikiiBTF3
GenomeRNAii689
PROiPR:P20290
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000145741 Expressed in 233 organ(s), highest expression level in thoracic mammary gland
CleanExiHS_BTF3
ExpressionAtlasiP20290 baseline and differential
GenevisibleiP20290 HS

Family and domain databases

Gene3Di2.20.70.30, 1 hit
InterProiView protein in InterPro
IPR038187 NAC_A/B_dom_sf
IPR002715 Nas_poly-pep-assoc_cplx_dom
PfamiView protein in Pfam
PF01849 NAC, 1 hit
SMARTiView protein in SMART
SM01407 NAC, 1 hit
PROSITEiView protein in PROSITE
PS51151 NAC_AB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBTF3_HUMAN
AccessioniPrimary (citable) accession number: P20290
Secondary accession number(s): A8K510, Q13893, Q76M56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 167 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again