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Protein

50S ribosomal protein L2

Gene

rpl2

Organism
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome (By similarity).By similarity

Miscellaneous

This protein can be partially incorporated into E.coli polysomes in vivo, indicating it can replace the endogenous protein.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L2
Alternative name(s):
Hl4
Hmal2
Gene namesi
Name:rpl2
Ordered Locus Names:rrnAC1608
OrganismiHaloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809) (Halobacterium marismortui)
Taxonomic identifieri272569 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaStenosarchaea groupHalobacteriaHalobacterialesHaloarculaceaeHaloarcula
Proteomesi
  • UP000001169 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi200H → A or G: No incorporation into translating E.coli polysomes; ribosomes assemble normally. Significantly reduced translational activity. 1 Publication1

Chemistry databases

DrugBankiDB04865 Omacetaxine mepesuccinate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001297122 – 24050S ribosomal protein L2Add BLAST239

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome (By similarity). Interacts weakly with protein L37Ae.By similarity2 Publications

Protein-protein interaction databases

STRINGi272569.rrnAC1608

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20276
SMRiP20276
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20276

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG04067 Archaea
COG0090 LUCA
HOGENOMiHOG000232983
KOiK02886
OMAiHGYIKGV
OrthoDBiPOG093Z08WO

Family and domain databases

Gene3Di2.30.30.30, 1 hit
4.10.950.10, 1 hit
HAMAPiMF_01320_A Ribosomal_L2_A, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022666 Rbsml_prot_L2_RNA-bd_dom
IPR014722 Rib_L2_dom2
IPR002171 Ribosomal_L2
IPR023672 Ribosomal_L2_arc
IPR022669 Ribosomal_L2_C
IPR022671 Ribosomal_L2_CS
IPR014726 Ribosomal_L2_dom3
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR13691 PTHR13691, 1 hit
PfamiView protein in Pfam
PF00181 Ribosomal_L2, 1 hit
PF03947 Ribosomal_L2_C, 1 hit
PIRSFiPIRSF002158 Ribosomal_L2, 1 hit
SMARTiView protein in SMART
SM01383 Ribosomal_L2, 1 hit
SM01382 Ribosomal_L2_C, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS00467 RIBOSOMAL_L2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P20276-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGRRIQGQRR GRGTSTFRAP SHRYKADLEH RKVEDGDVIA GTVVDIEHDP
60 70 80 90 100
ARSAPVAAVE FEDGDRRLIL APEGVGVGDE LQVGVSAEIA PGNTLPLAEI
110 120 130 140 150
PEGVPVCNVE SSPGDGGKFA RASGVNAQLL THDRNVAVVK LPSGEMKRLD
160 170 180 190 200
PQCRATIGVV AGGGRTDKPF VKAGNKHHKM KARGTKWPNV RGVAMNAVDH
210 220 230 240
PFGGGGRQHP GKPKSISRNA PPGRKVGDIA SKRTGRGGNE
Length:240
Mass (Da):25,309
Last modified:January 23, 2007 - v4
Checksum:i986CA69D432C7F74
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86S → D in AAA86862 (PubMed:2406244).Curated1
Sequence conflicti161A → G in AAA86862 (PubMed:2406244).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05222 Genomic DNA Translation: AAA86862.1
AY596297 Genomic DNA Translation: AAV46525.1
PIRiF35063 R5HS2L
RefSeqiWP_004957415.1, NC_006396.1

Genome annotation databases

EnsemblBacteriaiAAV46525; AAV46525; rrnAC1608
GeneIDi3128427
KEGGihma:rrnAC1608
PATRICifig|272569.17.peg.2298

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05222 Genomic DNA Translation: AAA86862.1
AY596297 Genomic DNA Translation: AAV46525.1
PIRiF35063 R5HS2L
RefSeqiWP_004957415.1, NC_006396.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1C04X-ray5.00A46-145[»]
1FFKX-ray2.40A2-240[»]
1JJ2X-ray2.40A2-240[»]
1K73X-ray3.01C2-240[»]
1K8AX-ray3.00C2-240[»]
1K9MX-ray3.00C2-240[»]
1KC8X-ray3.01C2-240[»]
1KD1X-ray3.00C2-240[»]
1KQSX-ray3.10A2-240[»]
1M1KX-ray3.20C2-240[»]
1M90X-ray2.80C2-240[»]
1N8RX-ray3.00C2-240[»]
1NJIX-ray3.00C2-240[»]
1Q7YX-ray3.20C2-240[»]
1Q81X-ray2.95C2-240[»]
1Q82X-ray2.98C2-240[»]
1Q86X-ray3.00C2-240[»]
1QVFX-ray3.10A2-240[»]
1QVGX-ray2.90A2-240[»]
1S72X-ray2.40A1-240[»]
1VQ4X-ray2.70A1-240[»]
1VQ5X-ray2.60A1-240[»]
1VQ6X-ray2.70A1-240[»]
1VQ7X-ray2.50A1-240[»]
1VQ8X-ray2.20A1-240[»]
1VQ9X-ray2.40A1-240[»]
1VQKX-ray2.30A1-240[»]
1VQLX-ray2.30A1-240[»]
1VQMX-ray2.30A1-240[»]
1VQNX-ray2.40A1-240[»]
1VQOX-ray2.20A1-240[»]
1VQPX-ray2.25A1-240[»]
1W2BX-ray3.50A2-240[»]
1YHQX-ray2.40A1-240[»]
1YI2X-ray2.65A1-240[»]
1YIJX-ray2.60A1-240[»]
1YITX-ray2.80A1-240[»]
1YJ9X-ray2.90A1-240[»]
1YJNX-ray3.00A1-240[»]
1YJWX-ray2.90A1-240[»]
2OTJX-ray2.90A1-240[»]
2OTLX-ray2.70A2-240[»]
2QA4X-ray3.00A1-240[»]
2QEXX-ray2.90A1-240[»]
3CC2X-ray2.40A1-240[»]
3CC4X-ray2.70A1-240[»]
3CC7X-ray2.70A1-240[»]
3CCEX-ray2.75A1-240[»]
3CCJX-ray2.70A1-240[»]
3CCLX-ray2.90A1-240[»]
3CCMX-ray2.55A1-240[»]
3CCQX-ray2.90A1-240[»]
3CCRX-ray3.00A1-240[»]
3CCSX-ray2.95A1-240[»]
3CCUX-ray2.80A1-240[»]
3CCVX-ray2.90A1-240[»]
3CD6X-ray2.75A1-240[»]
3CMAX-ray2.80A1-240[»]
3CMEX-ray2.95A1-240[»]
3CPWX-ray2.70A1-240[»]
3CXCX-ray3.00A2-240[»]
3G4SX-ray3.20A2-238[»]
3G6EX-ray2.70A2-238[»]
3G71X-ray2.85A2-238[»]
3I55X-ray3.11A1-240[»]
3I56X-ray2.90A1-240[»]
3OW2X-ray2.70A2-238[»]
4ADXelectron microscopy6.60A1-240[»]
4V9FX-ray2.40A1-240[»]
ProteinModelPortaliP20276
SMRiP20276
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272569.rrnAC1608

Chemistry databases

DrugBankiDB04865 Omacetaxine mepesuccinate

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV46525; AAV46525; rrnAC1608
GeneIDi3128427
KEGGihma:rrnAC1608
PATRICifig|272569.17.peg.2298

Phylogenomic databases

eggNOGiarCOG04067 Archaea
COG0090 LUCA
HOGENOMiHOG000232983
KOiK02886
OMAiHGYIKGV
OrthoDBiPOG093Z08WO

Miscellaneous databases

EvolutionaryTraceiP20276

Family and domain databases

Gene3Di2.30.30.30, 1 hit
4.10.950.10, 1 hit
HAMAPiMF_01320_A Ribosomal_L2_A, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR022666 Rbsml_prot_L2_RNA-bd_dom
IPR014722 Rib_L2_dom2
IPR002171 Ribosomal_L2
IPR023672 Ribosomal_L2_arc
IPR022669 Ribosomal_L2_C
IPR022671 Ribosomal_L2_CS
IPR014726 Ribosomal_L2_dom3
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR13691 PTHR13691, 1 hit
PfamiView protein in Pfam
PF00181 Ribosomal_L2, 1 hit
PF03947 Ribosomal_L2_C, 1 hit
PIRSFiPIRSF002158 Ribosomal_L2, 1 hit
SMARTiView protein in SMART
SM01383 Ribosomal_L2, 1 hit
SM01382 Ribosomal_L2_C, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS00467 RIBOSOMAL_L2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRL2_HALMA
AccessioniPrimary (citable) accession number: P20276
Secondary accession number(s): Q5V1S7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 138 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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