UniProtKB - P20265 (PO3F2_HUMAN)
Protein
POU domain, class 3, transcription factor 2
Gene
POU3F2
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 354 – 413 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: Ensembl
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- identical protein binding Source: IntAct
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- astrocyte development Source: Ensembl
- cellular response to organic substance Source: Ensembl
- cerebral cortex radially oriented cell migration Source: Ensembl
- epidermis development Source: Ensembl
- forebrain ventricular zone progenitor cell division Source: Ensembl
- hypothalamus cell differentiation Source: Ensembl
- myelination in peripheral nervous system Source: Ensembl
- negative regulation of gene expression Source: Ensembl
- nervous system development Source: UniProtKB
- neurohypophysis development Source: Ensembl
- neuron development Source: UniProtKB
- neuron differentiation Source: UniProtKB
- neuron fate commitment Source: UniProtKB
- neuron fate specification Source: UniProtKB
- positive regulation of cell population proliferation Source: MGI
- positive regulation of multicellular organism growth Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of axonogenesis Source: Ensembl
- regulation of cell differentiation Source: Ensembl
- regulation of transcription by RNA polymerase II Source: UniProtKB
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Differentiation, Neurogenesis, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P20265 |
Reactomei | R-HSA-9619665, EGR2 and SOX10-mediated initiation of Schwann cell myelination |
SignaLinki | P20265 |
SIGNORi | P20265 |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 3, transcription factor 2Alternative name(s): Brain-specific homeobox/POU domain protein 2 Short name: Brain-2 Short name: Brn-2 Nervous system-specific octamer-binding transcription factor N-Oct-3 Octamer-binding protein 7 Short name: Oct-7 Octamer-binding transcription factor 7 Short name: OTF-7 |
Gene namesi | Name:POU3F2 Synonyms:BRN2, OCT7, OTF7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9215, POU3F2 |
MIMi | 600494, gene |
neXtProti | NX_P20265 |
VEuPathDBi | HostDB:ENSG00000184486.8 |
Subcellular locationi
Nucleus
Nucleus
- nucleoplasm Source: HPA
Other locations
- chromatin Source: NTNU_SB
- transcription regulator complex Source: Ensembl
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5454 |
OpenTargetsi | ENSG00000184486 |
PharmGKBi | PA33539 |
Miscellaneous databases
Pharosi | P20265, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4189 |
Genetic variation databases
BioMutai | POU3F2 |
DMDMi | 78100757 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000013495 | 1 – 443 | POU domain, class 3, transcription factor 2Add BLAST | 443 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 341 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P20265 |
MassIVEi | P20265 |
MaxQBi | P20265 |
PaxDbi | P20265 |
PeptideAtlasi | P20265 |
PRIDEi | P20265 |
ProteomicsDBi | 53737 [P20265-1] 53738 [P20265-2] 53739 [P20265-3] |
PTM databases
iPTMneti | P20265 |
PhosphoSitePlusi | P20265 |
Expressioni
Tissue specificityi
Expressed specifically in the neuroectodermal cell lineage.
Gene expression databases
Bgeei | ENSG00000184486, Expressed in subventricular zone (outer) (primate) and 83 other tissues |
Genevisiblei | P20265, HS |
Organism-specific databases
HPAi | ENSG00000184486, Tissue enriched (brain) |
Interactioni
Subunit structurei
Interacts with PQBP1 (PubMed:10332029). Interaction with ISL1 (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsP20265
With | #Exp. | IntAct |
---|---|---|
EP300 [Q09472] | 3 | EBI-1167176,EBI-447295 |
PAX3 [P23760] | 2 | EBI-1167176,EBI-1167564 |
itself | 2 | EBI-1167176,EBI-1167176 |
SOX10 [P56693] | 3 | EBI-1167176,EBI-1167533 |
TBP [P20226] | 2 | EBI-1167176,EBI-355371 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 111450, 13 interactors |
IntActi | P20265, 6 interactors |
STRINGi | 9606.ENSP00000329170 |
Chemistry databases
BindingDBi | P20265 |
Miscellaneous databases
RNActi | P20265, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 262 – 336 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 68 – 90 | Poly-GlyAdd BLAST | 23 | |
Compositional biasi | 125 – 149 | Poly-GlnAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000162208 |
HOGENOMi | CLU_013065_1_2_1 |
InParanoidi | P20265 |
OMAi | MVHAEPP |
OrthoDBi | 752252at2759 |
PhylomeDBi | P20265 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR016362, TF_POU_3 |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PIRSFi | PIRSF002629, Transcription_factor_POU, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative initiation. AlignAdd to basketIsoform N-OCT 3 (identifier: P20265-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQR
160 170 180 190 200
PPHLVHHAAN HHPGPGAWRS AAAAAHLPPS MGASNGGLLY SQPSFTVNGM
210 220 230 240 250
LGAGGQPAGL HHHGLRDAHD EPHHADHHPH PHSHPHQQPP PPPPPQGPPG
260 270 280 290 300
HPGAHHDPHS DEDTPTSDDL EQFAKQFKQR RIKLGFTQAD VGLALGTLYG
310 320 330 340 350
NVFSQTTICR FEALQLSFKN MCKLKPLLNK WLEEADSSSG SPTSIDKIAA
360 370 380 390 400
QGRKRKKRTS IEVSVKGALE SHFLKCPKPS AQEITSLADS LQLEKEVVRV
410 420 430 440
WFCNRRQKEK RMTPPGGTLP GAEDVYGGSR DTPPHHGVQT PVQ
Isoform N-OCT 5A (identifier: P20265-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-180: Missing.
Isoform N-OCT 5B (identifier: P20265-3) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
1-199: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | G → A in CAA77990 (PubMed:8441633).Curated | 1 | |
Sequence conflicti | 170 | S → T (PubMed:8441633).Curated | 1 | |
Sequence conflicti | 170 | S → T (PubMed:7651733).Curated | 1 | |
Sequence conflicti | 207 | P → S in AAH51699 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018750 | 1 – 199 | Missing in isoform N-OCT 5B. CuratedAdd BLAST | 199 | |
Alternative sequenceiVSP_018749 | 1 – 180 | Missing in isoform N-OCT 5A. CuratedAdd BLAST | 180 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11933 mRNA Translation: CAA77990.1 L37868 Genomic DNA Translation: AAB59611.1 AL022395 Genomic DNA No translation available. BC051699 mRNA Translation: AAH51699.2 |
CCDSi | CCDS5040.1 [P20265-1] |
PIRi | S29334 |
RefSeqi | NP_005595.2, NM_005604.3 [P20265-1] |
Genome annotation databases
Ensembli | ENST00000328345; ENSP00000329170; ENSG00000184486 [P20265-1] |
GeneIDi | 5454 |
KEGGi | hsa:5454 |
UCSCi | uc003ppe.4, human [P20265-1] |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z11933 mRNA Translation: CAA77990.1 L37868 Genomic DNA Translation: AAB59611.1 AL022395 Genomic DNA No translation available. BC051699 mRNA Translation: AAH51699.2 |
CCDSi | CCDS5040.1 [P20265-1] |
PIRi | S29334 |
RefSeqi | NP_005595.2, NM_005604.3 [P20265-1] |
3D structure databases
SMRi | P20265 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 111450, 13 interactors |
IntActi | P20265, 6 interactors |
STRINGi | 9606.ENSP00000329170 |
Chemistry databases
BindingDBi | P20265 |
ChEMBLi | CHEMBL4189 |
PTM databases
iPTMneti | P20265 |
PhosphoSitePlusi | P20265 |
Genetic variation databases
BioMutai | POU3F2 |
DMDMi | 78100757 |
Proteomic databases
jPOSTi | P20265 |
MassIVEi | P20265 |
MaxQBi | P20265 |
PaxDbi | P20265 |
PeptideAtlasi | P20265 |
PRIDEi | P20265 |
ProteomicsDBi | 53737 [P20265-1] 53738 [P20265-2] 53739 [P20265-3] |
Protocols and materials databases
Antibodypediai | 18892, 353 antibodies |
Genome annotation databases
Ensembli | ENST00000328345; ENSP00000329170; ENSG00000184486 [P20265-1] |
GeneIDi | 5454 |
KEGGi | hsa:5454 |
UCSCi | uc003ppe.4, human [P20265-1] |
Organism-specific databases
CTDi | 5454 |
DisGeNETi | 5454 |
GeneCardsi | POU3F2 |
HGNCi | HGNC:9215, POU3F2 |
HPAi | ENSG00000184486, Tissue enriched (brain) |
MIMi | 600494, gene |
neXtProti | NX_P20265 |
OpenTargetsi | ENSG00000184486 |
PharmGKBi | PA33539 |
VEuPathDBi | HostDB:ENSG00000184486.8 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000162208 |
HOGENOMi | CLU_013065_1_2_1 |
InParanoidi | P20265 |
OMAi | MVHAEPP |
OrthoDBi | 752252at2759 |
PhylomeDBi | P20265 |
TreeFami | TF316413 |
Enzyme and pathway databases
PathwayCommonsi | P20265 |
Reactomei | R-HSA-9619665, EGR2 and SOX10-mediated initiation of Schwann cell myelination |
SignaLinki | P20265 |
SIGNORi | P20265 |
Miscellaneous databases
BioGRID-ORCSi | 5454, 14 hits in 1012 CRISPR screens |
GeneWikii | POU3F2 |
GenomeRNAii | 5454 |
Pharosi | P20265, Tbio |
PROi | PR:P20265 |
RNActi | P20265, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000184486, Expressed in subventricular zone (outer) (primate) and 83 other tissues |
Genevisiblei | P20265, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR016362, TF_POU_3 |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PIRSFi | PIRSF002629, Transcription_factor_POU, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PO3F2_HUMAN | |
Accessioni | P20265Primary (citable) accession number: P20265 Secondary accession number(s): Q14960, Q86V54, Q9UJL0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | September 13, 2005 | |
Last modified: | April 7, 2021 | |
This is version 210 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families