UniProtKB - P20263 (PO5F1_MOUSE)
POU domain, class 5, transcription factor 1
Pou5f1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 150 | DNACombined sources1 Publication | 1 | |
Binding sitei | 157 | DNACombined sources1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 223 – 282 | HomeoboxPROSITE-ProRule annotation1 PublicationAdd BLAST | 60 |
GO - Molecular functioni
- chromatin binding Source: MGI
- chromatin DNA binding Source: MGI
- cis-regulatory region sequence-specific DNA binding Source: AgBase
- cytokine binding Source: AgBase
- DNA binding Source: MGI
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription factor activity Source: UniProtKB
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCL
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
- HMG box domain binding Source: MGI
- miRNA binding Source: MGI
- POU domain binding Source: MGI
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SB
- RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific DNA binding Source: UniProtKB
- sequence-specific double-stranded DNA binding Source: MGI
- transcription factor binding Source: UniProtKB
- transcription regulatory region sequence-specific DNA binding Source: UniProtKB
- ubiquitin protein ligase binding Source: BHF-UCL
GO - Biological processi
- blastocyst development Source: BHF-UCL
- blastocyst growth Source: MGI
- BMP signaling pathway involved in heart induction Source: MGI
- cardiac cell fate determination Source: MGI
- cell differentiation Source: GO_Central
- cell fate commitment Source: MGI
- cell fate commitment involved in formation of primary germ layer Source: MGI
- cellular response to leukemia inhibitory factor Source: MGI
- ectodermal cell fate commitment Source: MGI
- endodermal cell fate commitment Source: MGI
- endodermal cell fate specification Source: MGI
- germ-line stem cell population maintenance Source: MGI
- mesodermal cell fate commitment Source: MGI
- negative regulation of calcium ion-dependent exocytosis Source: MGI
- negative regulation of cell differentiation Source: MGI
- negative regulation of gene expression Source: AgBase
- negative regulation of gene silencing by miRNA Source: MGI
- negative regulation of pri-miRNA transcription by RNA polymerase II Source: MGI
- negative regulation of protein kinase B signaling Source: MGI
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of canonical Wnt signaling pathway Source: MGI
- positive regulation of gene expression Source: AgBase
- positive regulation of SMAD protein signal transduction Source: MGI
- positive regulation of transcription, DNA-templated Source: MGI
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
- positive regulation of transcription initiation from RNA polymerase II promoter Source: CACAO
- regulation of asymmetric cell division Source: BHF-UCL
- regulation of DNA methylation-dependent heterochromatin assembly Source: MGI
- regulation of gene expression Source: MGI
- regulation of heart induction by regulation of canonical Wnt signaling pathway Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: BHF-UCL
- response to organic substance Source: MGI
- response to retinoic acid Source: MGI
- somatic stem cell population maintenance Source: MGI
- stem cell differentiation Source: MGI
- stem cell population maintenance Source: UniProtKB
- transcription by RNA polymerase II Source: MGI
- trophectodermal cell differentiation Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: POU domain, class 5, transcription factor 1Alternative name(s): NF-A3 Octamer-binding protein 3 Short name: Oct-3 Octamer-binding protein 4 Short name: Oct-4 Octamer-binding transcription factor 3 Short name: OTF-3 |
Gene namesi | Name:Pou5f1 Synonyms:Oct-3, Oct-4, Otf-3, Otf3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:101893, Pou5f1 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nuclear chromatin Source: AgBase
- nucleolus Source: MGI
- nucleoplasm Source: BHF-UCL
- nucleus Source: BHF-UCL
Other locations
- cytoplasm Source: MGI
- transcription regulator complex Source: MGI
- transcription repressor complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Biotechnological usei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 118 | K → R: Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 70% lower expression of YES1. Reduced DNA binding. No change in nuclear location. No change in nuclear localization. Absence of sumoylation; when associated with R-215 and R-244. 3 Publications | 1 | |
Mutagenesisi | 120 | E → A: Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 55% lower expression of YES1. 1 Publication | 1 | |
Mutagenesisi | 122 – 352 | Missing : Loss of MAPK9 binding. Absence of Ser-347 phosphorylation. 1 PublicationAdd BLAST | 231 | |
Mutagenesisi | 166 | V → K: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 206 – 222 | NNENL…TLVQA → SSSGSPTNLDKIAAQGR: Reduced DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. 1 PublicationAdd BLAST | 17 | |
Mutagenesisi | 206 – 210 | NNENL → AAAAA: Loss of self-renewal ability in iPS cells. 1 Publication | 5 | |
Mutagenesisi | 206 | N → A: No change in DNA binding. Reduced self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 207 | N → A: No change in DNA binding. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 208 | E → A: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 209 | N → A: No change in DNA binding. Reduced self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 210 | L → A: No change in DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. No loss of ability to bind SOX2. Reduced levels of CHD4 and SMARCA4 in a POU5F1 pulldown assay. 1 Publication | 1 | |
Mutagenesisi | 211 | Q → A: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 211 | Q → R: No change in DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 212 | E → A: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication | 1 | |
Mutagenesisi | 215 | K → R: No change in sumoylation; when associated with R-244. Loss of sumoylation. No change in nuclear localization; when associated with R-118 and R-244. 1 Publication | 1 | |
Mutagenesisi | 244 | K → R: No change in sumoylation. No change in sumoylation; when associated with R-215. Loss of sumoylation; when associated with R-118 and R-215. No change in nuclear localization; when associated with R-118 and R-215. 1 Publication | 1 | |
Mutagenesisi | 347 | S → A: Absence of phosphorylation. Reduced protein degradation. Reduced self-renewal ability in ES cells. No change in FBXW4 binding. Loss of FBXW8 binding. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000100749 | 1 – 352 | POU domain, class 5, transcription factor 1Add BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 106 | Phosphoserine; by MAPKBy similarity | 1 | |
Cross-linki | 118 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)3 Publications | ||
Modified residuei | 228 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 229 | PhosphoserineBy similarity | 1 | |
Modified residuei | 282 | PhosphoserineBy similarity | 1 | |
Modified residuei | 347 | Phosphoserine; by MAPK8 and MAPK91 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P20263 |
PeptideAtlasi | P20263 |
PRIDEi | P20263 |
PTM databases
iPTMneti | P20263 |
PhosphoSitePlusi | P20263 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000024406, Expressed in morula and 94 other tissues |
ExpressionAtlasi | P20263, baseline and differential |
Genevisiblei | P20263, MM |
Interactioni
Subunit structurei
Interacts with PKM.
Interacts with WWP2 (By similarity).
Interacts with UBE2I and ZSCAN10 (PubMed:17496161, PubMed:19740739).
Interacts with PCGF1 (By similarity).
Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (PubMed:18662995).
Interacts with ZNF322 (PubMed:24550733).
Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-347 (PubMed:29153991).
Interacts (when phosphorylated on Ser-347) with FBXW8 (PubMed:29153991).
Interacts with FBXW4 (PubMed:29153991).
Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (PubMed:15863505).
By similarity6 PublicationsBinary interactionsi
Hide detailsP20263
GO - Molecular functioni
- cytokine binding Source: AgBase
- HMG box domain binding Source: MGI
- POU domain binding Source: MGI
- transcription factor binding Source: UniProtKB
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 202313, 304 interactors |
DIPi | DIP-29931N |
IntActi | P20263, 141 interactors |
MINTi | P20263 |
STRINGi | 10090.ENSMUSP00000025271 |
Miscellaneous databases
RNActi | P20263, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P20263 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20263 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 131 – 205 | POU-specificPROSITE-ProRule annotationAdd BLAST | 75 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 173 – 179 | DNA bindingCombined sources1 Publication | 7 | |
Regioni | 186 – 189 | DNA bindingCombined sources1 Publication | 4 |
Domaini
Sequence similaritiesi
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000155046 |
HOGENOMi | CLU_066243_0_0_1 |
InParanoidi | P20263 |
OMAi | CPQPYEF |
OrthoDBi | 841019at2759 |
PhylomeDBi | P20263 |
TreeFami | TF316413 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR015585, POU_dom_5 |
PANTHERi | PTHR11636:SF86, PTHR11636:SF86, 2 hits |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAGHLASDFA FSPPPGGGDG SAGLEPGWVD PRTWLSFQGP PGGPGIGPGS
60 70 80 90 100
EVLGISPCPP AYEFCGGMAY CGPQVGLGLV PQVGVETLQP EGQAGARVES
110 120 130 140 150
NSEGTSSEPC ADRPNAVKLE KVEPTPEESQ DMKALQKELE QFAKLLKQKR
160 170 180 190 200
ITLGYTQADV GLTLGVLFGK VFSQTTICRF EALQLSLKNM CKLRPLLEKW
210 220 230 240 250
VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET MFLKCPKPSL
260 270 280 290 300
QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG
310 320 330 340 350
GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH
SN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE2JL30 | E2JL30_MOUSE | Octamer-binding transcription facto... | Pou5f1 Oct-3/4 | 132 | Annotation score: | ||
G3UZG9 | G3UZG9_MOUSE | Oct-3/4 protein | Pou5f1 Oct-3/4 | 157 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 28 | Missing in CAA36682 (PubMed:1690859).CuratedAdd BLAST | 28 | |
Sequence conflicti | 29 | V → M in CAA36682 (PubMed:1690859).Curated | 1 | |
Sequence conflicti | 31 | P → S in AAA39844 (PubMed:1915274).Curated | 1 | |
Sequence conflicti | 31 | P → S in AAB19896 (PubMed:1915274).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34381 mRNA Translation: AAA39844.1 Sequence problems. X52437 mRNA Translation: CAA36682.1 S58426 , S58422, S58423, S58424, S58425 Genomic DNA Translation: AAB19896.1 BC068268 mRNA Translation: AAH68268.1 |
CCDSi | CCDS37600.1 |
PIRi | A34672 S17313 |
RefSeqi | NP_038661.2, NM_013633.3 |
Genome annotation databases
Ensembli | ENSMUST00000025271; ENSMUSP00000025271; ENSMUSG00000024406 |
GeneIDi | 18999 |
KEGGi | mmu:18999 |
UCSCi | uc008chu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34381 mRNA Translation: AAA39844.1 Sequence problems. X52437 mRNA Translation: CAA36682.1 S58426 , S58422, S58423, S58424, S58425 Genomic DNA Translation: AAB19896.1 BC068268 mRNA Translation: AAH68268.1 |
CCDSi | CCDS37600.1 |
PIRi | A34672 S17313 |
RefSeqi | NP_038661.2, NM_013633.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OCP | NMR | - | A | 217-282 | [»] | |
3L1P | X-ray | 2.80 | A/B | 131-282 | [»] | |
6HT5 | X-ray | 3.45 | E | 131-282 | [»] | |
SMRi | P20263 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 202313, 304 interactors |
DIPi | DIP-29931N |
IntActi | P20263, 141 interactors |
MINTi | P20263 |
STRINGi | 10090.ENSMUSP00000025271 |
PTM databases
iPTMneti | P20263 |
PhosphoSitePlusi | P20263 |
Proteomic databases
PaxDbi | P20263 |
PeptideAtlasi | P20263 |
PRIDEi | P20263 |
Genome annotation databases
Ensembli | ENSMUST00000025271; ENSMUSP00000025271; ENSMUSG00000024406 |
GeneIDi | 18999 |
KEGGi | mmu:18999 |
UCSCi | uc008chu.2, mouse |
Organism-specific databases
CTDi | 5460 |
MGIi | MGI:101893, Pou5f1 |
Phylogenomic databases
eggNOGi | KOG3802, Eukaryota |
GeneTreei | ENSGT00940000155046 |
HOGENOMi | CLU_066243_0_0_1 |
InParanoidi | P20263 |
OMAi | CPQPYEF |
OrthoDBi | 841019at2759 |
PhylomeDBi | P20263 |
TreeFami | TF316413 |
Miscellaneous databases
BioGRID-ORCSi | 18999, 4 hits in 18 CRISPR screens |
ChiTaRSi | Pou5f1, mouse |
EvolutionaryTracei | P20263 |
PROi | PR:P20263 |
RNActi | P20263, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024406, Expressed in morula and 94 other tissues |
ExpressionAtlasi | P20263, baseline and differential |
Genevisiblei | P20263, MM |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR010982, Lambda_DNA-bd_dom_sf IPR013847, POU IPR000327, POU_dom IPR015585, POU_dom_5 |
PANTHERi | PTHR11636:SF86, PTHR11636:SF86, 2 hits |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit PF00157, Pou, 1 hit |
PRINTSi | PR00028, POUDOMAIN |
SMARTi | View protein in SMART SM00389, HOX, 1 hit SM00352, POU, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit PS00035, POU_1, 1 hit PS00465, POU_2, 1 hit PS51179, POU_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PO5F1_MOUSE | |
Accessioni | P20263Primary (citable) accession number: P20263 Secondary accession number(s): Q63843 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families