POU domain, class 5, transcription factor 1

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functionⁱ

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functionⁱ

• chromatin binding Source: MGI <p>Inferred from Direct Assay</p> <p>Used to indicate a direct assay for the function, process or component indicated by the GO term.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ida">GO evidence code guide</a></p> Inferred from direct assayⁱ
  • "Reciprocal transcriptional regulation of Pou5f1 and Sox2 via the Oct4/Sox2 complex in embryonic stem cells."
    Chew J.L., Loh Y.H., Zhang W., Chen X., Tam W.L., Yeap L.S., Li P., Ang Y.S., Lim B., Robson P., Ng H.H.
    Mol. Cell. Biol. 25:6031-6046(2005) [PubMed] [Europe PMC] [Abstract]
  • "T-cell factor 3 regulates embryonic stem cell pluripotency and self-renewal by the transcriptional control of multiple lineage pathways."
    Tam W.L., Lim C.Y., Han J., Zhang J., Ang Y.S., Ng H.H., Yang H., Lim B.
    Stem Cells 26:2019-2031(2008) [PubMed] [Europe PMC] [Abstract]
  • "Tet1 and Tet2 regulate 5-hydroxymethylcytosine production and cell lineage specification in mouse embryonic stem cells."
    Koh K.P., Yabuuchi A., Rao S., Huang Y., Cunniff K., Nardone J., Laiho A., Tahiliani M., Sommer C.A., Mostoslavsky G., Lahesmaa R., Orkin S.H., Rodig S.J., Daley G.Q., Rao A.
    Cell Stem Cell 8:200-213(2011) [PubMed] [Europe PMC] [Abstract]
• chromatin DNA binding Source: MGIInferred from direct assayⁱ
  • "Tsix RNA and the germline factor, PRDM14, link X reactivation and stem cell reprogramming."
    Payer B., Rosenberg M., Yamaji M., Yabuta Y., Koyanagi-Aoi M., Hayashi K., Yamanaka S., Saitou M., Lee J.T.
    Mol. Cell 52:805-818(2013) [PubMed] [Europe PMC] [Abstract]
• cis-regulatory region sequence-specific DNA binding Source: AgBaseInferred from direct assayⁱ
  • "Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency."
    Campbell P.A., Rudnicki M.A.
    Stem Cells 31:1107-1120(2013) [PubMed] [Europe PMC] [Abstract]
• cytokine binding Source: AgBaseInferred from direct assayⁱ
  • "Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency."
    Campbell P.A., Rudnicki M.A.
    Stem Cells 31:1107-1120(2013) [PubMed] [Europe PMC] [Abstract]
• DNA binding Source: MGIInferred from direct assayⁱ
  • Ref.3

    "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells."
    Okamoto K., Okazawa H., Okuda A., Sakai M., Muramatsu M., Hamada H.
    Cell 60:461-472(1990) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
• DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SBInferred from direct assayⁱ
  • "The embryonic Octamer factor 3/4 displays distinct DNA binding specificity from those of other Octamer factors."
    Nishimoto M., Miyagi S., Katayanagi T., Tomioka M., Muramatsu M., Okuda A.
    Biochem. Biophys. Res. Commun. 302:581-586(2003) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity Source: UniProtKBInferred from direct assayⁱ
  • "Dax1 associates with Esrrb and regulates its function in embryonic stem cells."
    Uranishi K., Akagi T., Sun C., Koide H., Yokota T.
    Mol. Cell. Biol. 33:2056-2066(2013) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription factor activity, RNA polymerase II-specific Source: BHF-UCLInferred from direct assayⁱ
  • "Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination."
    Xu H.M., Liao B., Zhang Q.J., Wang B.B., Li H., Zhong X.M., Sheng H.Z., Zhao Y.X., Zhao Y.M., Jin Y.
    J. Biol. Chem. 279:23495-23503(2004) [PubMed] [Europe PMC] [Abstract]
• DNA-binding transcription repressor activity, RNA polymerase II-specific Source: MGI
• HMG box domain binding Source: MGI
• miRNA binding Source: MGI
• POU domain binding Source: MGI
• RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: NTNU_SBInferred from direct assayⁱ
  • "The embryonic Octamer factor 3/4 displays distinct DNA binding specificity from those of other Octamer factors."
    Nishimoto M., Miyagi S., Katayanagi T., Tomioka M., Muramatsu M., Okuda A.
    Biochem. Biophys. Res. Commun. 302:581-586(2003) [PubMed] [Europe PMC] [Abstract]
• RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: MGIInferred from direct assayⁱ
  • "Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells."
    Loh Y.-H., Zhang W., Chen X., George J., Ng H.H.
    Genes Dev. 21:2545-2557(2007) [PubMed] [Europe PMC] [Abstract]
• sequence-specific DNA binding Source: UniProtKBInferred from direct assayⁱ
  • "Dax1 associates with Esrrb and regulates its function in embryonic stem cells."
    Uranishi K., Akagi T., Sun C., Koide H., Yokota T.
    Mol. Cell. Biol. 33:2056-2066(2013) [PubMed] [Europe PMC] [Abstract]
• transcription coactivator activity Source: MGI
• transcription corepressor activity Source: MGIInferred from direct assayⁱ
  • "Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation."
    Niwa H., Toyooka Y., Shimosato D., Strumpf D., Takahashi K., Yagi R., Rossant J.
    Cell 123:917-929(2005) [PubMed] [Europe PMC] [Abstract]
• transcription factor binding Source: UniProtKB <p>Inferred from Physical Interaction</p> <p>Covers physical interactions between the gene product of interest and another molecule (or ion, or complex).</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#ipi">GO evidence code guide</a></p> Inferred from physical interactionⁱ
  • "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
    Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
    EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
  • "Klf4 interacts directly with Oct4 and Sox2 to promote reprogramming."
    Wei Z., Yang Y., Zhang P., Andrianakos R., Hasegawa K., Lyu J., Chen X., Bai G., Liu C., Pera M., Lu W.
    Stem Cells 27:2969-2978(2009) [PubMed] [Europe PMC] [Abstract]
• transcription regulatory region DNA binding Source: UniProtKBInferred from direct assayⁱ
  • "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
    Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
    EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
• transcription regulatory region sequence-specific DNA binding Source: MGI
• ubiquitin protein ligase binding Source: BHF-UCLInferred from physical interactionⁱ
  • "Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination."
    Xu H.M., Liao B., Zhang Q.J., Wang B.B., Li H., Zhong X.M., Sheng H.Z., Zhao Y.X., Zhao Y.M., Jin Y.
    J. Biol. Chem. 279:23495-23503(2004) [PubMed] [Europe PMC] [Abstract]

GO - Biological processⁱ

• blastocyst development Source: BHF-UCL <p>Inferred from Expression Pattern</p> <p>Covers cases where the annotation is inferred from the timing or location of expression of a gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iep">GO evidence code guide</a></p> Inferred from expression patternⁱ
  • "Oct4 kinetics predict cell lineage patterning in the early mammalian embryo."
    Plachta N., Bollenbach T., Pease S., Fraser S.E., Pantazis P.
    Nat. Cell Biol. 13:117-123(2011) [PubMed] [Europe PMC] [Abstract]
• blastocyst growth Source: MGI <p>Inferred from Genetic Interaction</p> <p>Used to describe "traditional" genetic interactions such as suppressors and synthetic lethals as well as other techniques such as functional complementation, rescue experiments, or inferences about a gene drawn from the phenotype of a mutation in a different gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#igi">GO evidence code guide</a></p> Inferred from genetic interactionⁱ
  • "CTR9/PAF1c regulates molecular lineage identity, histone H3K36 trimethylation and genomic imprinting during preimplantation development."
    Zhang K., Haversat J.M., Mager J.
    Dev. Biol. 383:15-27(2013) [PubMed] [Europe PMC] [Abstract]
• BMP signaling pathway involved in heart induction Source: MGI
• cardiac cell fate determination Source: MGI
• cell differentiation Source: GO_Central <p>Inferred from Biological aspect of Ancestor</p> <p>A type of phylogenetic evidence whereby an aspect of a descendent is inferred through the characterization of an aspect of a ancestral gene.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#iba">GO evidence code guide</a></p> Inferred from biological aspect of ancestorⁱ
  • "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium."
    Gaudet P., Livstone M.S., Lewis S.E., Thomas P.D.
    Brief. Bioinformatics 12:449-462(2011) [PubMed] [Europe PMC] [Abstract]
• cell fate commitment Source: MGI <p>Inferred from Mutant Phenotype</p> <p>Describes annotations that are concluded from looking at variations or changes in a gene product such as mutations or abnormal levels and includes techniques such as knockouts, overexpression, anti-sense experiments and use of specific protein inhibitors.</p> <p>More information in the <a href="http://geneontology.org/page/guide%2Dgo%2Devidence%2Dcodes#imp">GO evidence code guide</a></p> Inferred from mutant phenotypeⁱ
  • "Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4."
    Nichols J., Zevnik B., Anastassiadis K., Niwa H., Klewe-Nebenius D., Chambers I., Scholer H., Smith A.
    Cell 95:379-391(1998) [PubMed] [Europe PMC] [Abstract]
• cell fate commitment involved in formation of primary germ layer Source: MGI
• cellular response to leukemia inhibitory factor Source: MGIInferred from expression patternⁱ
  • "miRNA 34a, 100, and 137 modulate differentiation of mouse embryonic stem cells."
    Tarantino C., Paolella G., Cozzuto L., Minopoli G., Pastore L., Parisi S., Russo T.
    FASEB J. 24:3255-3263(2010) [PubMed] [Europe PMC] [Abstract]
• ectodermal cell fate commitment Source: MGIInferred from direct assayⁱ
  • "Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells."
    Niwa H., Miyazaki J., Smith A.G.
    Nat. Genet. 24:372-376(2000) [PubMed] [Europe PMC] [Abstract]
• endodermal cell fate commitment Source: MGIInferred from direct assayⁱ
  • "Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells."
    Niwa H., Miyazaki J., Smith A.G.
    Nat. Genet. 24:372-376(2000) [PubMed] [Europe PMC] [Abstract]
• endodermal cell fate specification Source: MGIInferred from sequence orthologyⁱ
  • "Pluripotency factors regulate definitive endoderm specification through eomesodermin."
    Teo A.K., Arnold S.J., Trotter M.W., Brown S., Ang L.T., Chng Z., Robertson E.J., Dunn N.R., Vallier L.
    Genes Dev. 25:238-250(2011) [PubMed] [Europe PMC] [Abstract]
• germ-line stem cell population maintenance Source: MGIInferred from mutant phenotypeⁱ
  • "Oct4 is required for primordial germ cell survival."
    Kehler J., Tolkunova E., Koschorz B., Pesce M., Gentile L., Boiani M., Lomeli H., Nagy A., McLaughlin K.J., Scholer H.R., Tomilin A.
    EMBO Rep. 5:1078-1083(2004) [PubMed] [Europe PMC] [Abstract]
• mesodermal cell fate commitment Source: MGIInferred from direct assayⁱ
  • "Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells."
    Niwa H., Miyazaki J., Smith A.G.
    Nat. Genet. 24:372-376(2000) [PubMed] [Europe PMC] [Abstract]
• mRNA transcription by RNA polymerase II Source: BHF-UCLInferred from direct assayⁱ
  • "Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination."
    Xu H.M., Liao B., Zhang Q.J., Wang B.B., Li H., Zhong X.M., Sheng H.Z., Zhao Y.X., Zhao Y.M., Jin Y.
    J. Biol. Chem. 279:23495-23503(2004) [PubMed] [Europe PMC] [Abstract]
• negative regulation of calcium ion-dependent exocytosis Source: MGIInferred from direct assayⁱ
  • "Oct-4: a germline-specific transcription factor mapping to the mouse t-complex."
    Scholer H.R., Dressler G.R., Balling R., Rohdewohld H., Gruss P.
    EMBO J. 9:2185-2195(1990) [PubMed] [Europe PMC] [Abstract]
• negative regulation of cell differentiation Source: MGIInferred from genetic interactionⁱ
  • "Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation."
    Niwa H., Toyooka Y., Shimosato D., Strumpf D., Takahashi K., Yagi R., Rossant J.
    Cell 123:917-929(2005) [PubMed] [Europe PMC] [Abstract]
• negative regulation of gene expression Source: AgBaseInferred from direct assayⁱ
  • "Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency."
    Campbell P.A., Rudnicki M.A.
    Stem Cells 31:1107-1120(2013) [PubMed] [Europe PMC] [Abstract]
• negative regulation of gene silencing by miRNA Source: MGI
• negative regulation of protein kinase B signaling Source: MGIInferred from mutant phenotypeⁱ
  • "Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency."
    Campbell P.A., Rudnicki M.A.
    Stem Cells 31:1107-1120(2013) [PubMed] [Europe PMC] [Abstract]
• negative regulation of transcription, DNA-templated Source: MGIInferred from direct assayⁱ
  • Ref.3

    "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells."
    Okamoto K., Okazawa H., Okuda A., Sakai M., Muramatsu M., Hamada H.
    Cell 60:461-472(1990) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
• negative regulation of transcription by RNA polymerase II Source: MGIInferred from genetic interactionⁱ
  • "Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation."
    Niwa H., Toyooka Y., Shimosato D., Strumpf D., Takahashi K., Yagi R., Rossant J.
    Cell 123:917-929(2005) [PubMed] [Europe PMC] [Abstract]
• positive regulation of canonical Wnt signaling pathway Source: MGI
• positive regulation of gene expression Source: AgBaseInferred from direct assayⁱ
  • "Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency."
    Campbell P.A., Rudnicki M.A.
    Stem Cells 31:1107-1120(2013) [PubMed] [Europe PMC] [Abstract]
• positive regulation of SMAD protein signal transduction Source: MGI
• positive regulation of transcription, DNA-templated Source: MGIInferred from direct assayⁱ
  • Ref.3

    "A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells."
    Okamoto K., Okazawa H., Okuda A., Sakai M., Muramatsu M., Hamada H.
    Cell 60:461-472(1990) [PubMed] [Europe PMC] [Abstract]

    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION.
• positive regulation of transcription by RNA polymerase II Source: NTNU_SBInferred from direct assayⁱ
  • "The embryonic Octamer factor 3/4 displays distinct DNA binding specificity from those of other Octamer factors."
    Nishimoto M., Miyagi S., Katayanagi T., Tomioka M., Muramatsu M., Okuda A.
    Biochem. Biophys. Res. Commun. 302:581-586(2003) [PubMed] [Europe PMC] [Abstract]
• positive regulation of transcription initiation from RNA polymerase II promoter Source: CACAOInferred from direct assayⁱ
  • "Physical Interactions and Functional Coordination between the Core Subunits of Set1/Mll Complexes and the Reprogramming Factors."
    Yang Z., Augustin J., Hu J., Jiang H.
    PLoS ONE 10:e0145336-e0145336(2015) [PubMed] [Europe PMC] [Abstract]
• regulation of asymmetric cell division Source: BHF-UCLInferred from expression patternⁱ
  • "Oct4 kinetics predict cell lineage patterning in the early mammalian embryo."
    Plachta N., Bollenbach T., Pease S., Fraser S.E., Pantazis P.
    Nat. Cell Biol. 13:117-123(2011) [PubMed] [Europe PMC] [Abstract]
• regulation of gene expression Source: MGI
• regulation of heart induction by regulation of canonical Wnt signaling pathway Source: MGI
• regulation of methylation-dependent chromatin silencing Source: MGI
• regulation of transcription, DNA-templated Source: MGIInferred from direct assayⁱ
  • "Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development."
    Tokuzawa Y., Kaiho E., Maruyama M., Takahashi K., Mitsui K., Maeda M., Niwa H., Yamanaka S.
    Mol. Cell. Biol. 23:2699-2708(2003) [PubMed] [Europe PMC] [Abstract]
• response to organic substance Source: MGIInferred from direct assayⁱ
  • "Detailed characterization of the mouse embryonic stem cell transcriptome reveals novel genes and intergenic splicing associated with pluripotency."
    Kunarso G., Wong K.Y., Stanton L.W., Lipovich L.
    BMC Genomics 9:155-155(2008) [PubMed] [Europe PMC] [Abstract]
• response to retinoic acid Source: MGIInferred from direct assayⁱ
  • "Detailed characterization of the mouse embryonic stem cell transcriptome reveals novel genes and intergenic splicing associated with pluripotency."
    Kunarso G., Wong K.Y., Stanton L.W., Lipovich L.
    BMC Genomics 9:155-155(2008) [PubMed] [Europe PMC] [Abstract]
• somatic stem cell population maintenance Source: MGI
• stem cell differentiation Source: MGIInferred from direct assayⁱ
  • "Sox17 promotes differentiation in mouse embryonic stem cells by directly regulating extraembryonic gene expression and indirectly antagonizing self-renewal."
    Niakan K.K., Ji H., Maehr R., Vokes S.A., Rodolfa K.T., Sherwood R.I., Yamaki M., Dimos J.T., Chen A.E., Melton D.A., McMahon A.P., Eggan K.
    Genes Dev. 24:312-326(2010) [PubMed] [Europe PMC] [Abstract]
• stem cell population maintenance Source: UniProtKBInferred from mutant phenotypeⁱ
  • Ref.17

    "A unique Oct4 interface is crucial for reprogramming to pluripotency."
    Esch D., Vahokoski J., Groves M.R., Pogenberg V., Cojocaru V., Vom Bruch H., Han D., Drexler H.C., Arauzo-Bravo M.J., Ng C.K., Jauch R., Wilmanns M., Scholer H.R.
    Nat. Cell Biol. 15:295-301(2013) [PubMed] [Europe PMC] [Abstract]

    Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 131-282 IN COMPLEX WITH DNA, FUNCTION, UBIQUITINATION, MUTAGENESIS OF VAL-166; ASN-206; 206-ASN--LEU-210; 206-ASN--ALA-222; ASN-207; 207-ASN--ALA-222; GLU-208; ASN-209; LEU-210; GLN-211; GLU-212 AND 214-CYS--ALA-222.
• transcription by RNA polymerase II Source: BHF-UCLInferred from direct assayⁱ
  • "Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination."
    Xu H.M., Liao B., Zhang Q.J., Wang B.B., Li H., Zhong X.M., Sheng H.Z., Zhao Y.X., Zhao Y.M., Jin Y.
    J. Biol. Chem. 279:23495-23503(2004) [PubMed] [Europe PMC] [Abstract]
• trophectodermal cell differentiation Source: MGIInferred from genetic interactionⁱ
  • "Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation."
    Niwa H., Toyooka Y., Shimosato D., Strumpf D., Takahashi K., Yagi R., Rossant J.
    Cell 123:917-929(2005) [PubMed] [Europe PMC] [Abstract]

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsⁱ

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyⁱ

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationⁱ

Keywords - Cellular componentⁱ

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechⁱ

<p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological useⁱ

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingⁱ

Molecule processing

Amino acid modifications

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationⁱ

Keywords - PTMⁱ

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressionⁱ

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityⁱ

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stageⁱ

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductionⁱ

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactionⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structureⁱ

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsⁱ

GO - Molecular functionⁱ

• cytokine binding Source: AgBaseInferred from direct assayⁱ
  • "Oct4 interaction with Hmgb2 regulates Akt signaling and pluripotency."
    Campbell P.A., Rudnicki M.A.
    Stem Cells 31:1107-1120(2013) [PubMed] [Europe PMC] [Abstract]
• HMG box domain binding Source: MGI
• POU domain binding Source: MGI
• transcription factor binding Source: UniProtKBInferred from physical interactionⁱ
  • "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling in Xenopus embryos."
    Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.
    EMBO J. 26:2942-2954(2007) [PubMed] [Europe PMC] [Abstract]
  • "Klf4 interacts directly with Oct4 and Sox2 to promote reprogramming."
    Wei Z., Yang Y., Zhang P., Andrianakos R., Hasegawa K., Lyu J., Chen X., Bai G., Liu C., Pera M., Lu W.
    Stem Cells 27:2969-2978(2009) [PubMed] [Europe PMC] [Abstract]
• ubiquitin protein ligase binding Source: BHF-UCLInferred from physical interactionⁱ
  • "Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination."
    Xu H.M., Liao B., Zhang Q.J., Wang B.B., Li H., Zhong X.M., Sheng H.Z., Zhao Y.X., Zhao Y.M., Jin Y.
    J. Biol. Chem. 279:23495-23503(2004) [PubMed] [Europe PMC] [Abstract]

Protein-protein interaction databases

Miscellaneous databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structureⁱ

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsⁱ

Domains and Repeats

Region

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domainⁱ

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesⁱ

Keywords - Domainⁱ

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)ⁱ

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

        10         20         30         40         50
MAGHLASDFA FSPPPGGGDG SAGLEPGWVD PRTWLSFQGP PGGPGIGPGS 
        60         70         80         90        100
EVLGISPCPP AYEFCGGMAY CGPQVGLGLV PQVGVETLQP EGQAGARVES 
       110        120        130        140        150
NSEGTSSEPC ADRPNAVKLE KVEPTPEESQ DMKALQKELE QFAKLLKQKR 
       160        170        180        190        200
ITLGYTQADV GLTLGVLFGK VFSQTTICRF EALQLSLKNM CKLRPLLEKW 
       210        220        230        240        250
VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET MFLKCPKPSL 
       260        270        280        290        300
QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG 
       310        320        330        340        350
GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH 

SN                                                     

Experimental Info

Sequence databases

Genome annotation databases

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsⁱ

• 100% Identity
• 90% Identity
• 50% Identity

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesⁱ

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationⁱ

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousⁱ

Keywords - Technical termⁱ

Documents

1. SIMILARITY comments
   Index of protein domains and families
2. MGD cross-references
   Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
3. PDB cross-references
   Index of Protein Data Bank (PDB) cross-references