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Protein

POU domain, class 5, transcription factor 1

Gene

Pou5f1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973).10 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei150DNACombined sources1 Publication1
Binding sitei157DNACombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi223 – 282HomeoboxPROSITE-ProRule annotation1 PublicationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-452723 Transcriptional regulation of pluripotent stem cells

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 5, transcription factor 1
Alternative name(s):
NF-A3
Octamer-binding protein 3
Short name:
Oct-3
Octamer-binding protein 4
Short name:
Oct-4
Octamer-binding transcription factor 3
Short name:
OTF-3
Gene namesi
Name:Pou5f1
Synonyms:Oct-3, Oct-4, Otf-3, Otf3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:101893 Pou5f1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Biotechnological usei

POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 are the four Yamanaka factors. When combined, these factors are sufficient to reprogram differentiated cells to an embryonic-like state designated iPS (induced pluripotent stem) cells. iPS cells exhibit the morphology and growth properties of ES cells and express ES cell marker genes.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi118K → R: Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 70% lower expression of YES1. Reduced DNA binding. No change in nuclear location. No change in nuclear localization. Absence of sumoylation; when associated with R-215 and R-244. 3 Publications1
Mutagenesisi120E → A: Absence of sumoylation. Enhanced protein degradation. Reduced self-renewal ability in ES cells. 55% lower expression of YES1. 1 Publication1
Mutagenesisi122 – 352Missing : Loss of MAPK9 binding. Absence of Ser-347 phosphorylation. 1 PublicationAdd BLAST231
Mutagenesisi166V → K: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi206 – 222NNENL…TLVQA → SSSGSPTNLDKIAAQGR: Reduced DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. 1 PublicationAdd BLAST17
Mutagenesisi206 – 210NNENL → AAAAA: Loss of self-renewal ability in iPS cells. 1 Publication5
Mutagenesisi206N → A: No change in DNA binding. Reduced self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi207N → A: No change in DNA binding. No change in nuclear location. 1 Publication1
Mutagenesisi208E → A: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi209N → A: No change in DNA binding. Reduced self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi210L → A: No change in DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. No loss of ability to bind SOX2. Reduced levels of CHD4 and SMARCA4 in a POU5F1 pulldown assay. 1 Publication1
Mutagenesisi211Q → A: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi211Q → R: No change in DNA binding. Loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi212E → A: No change in DNA binding. No loss of self-renewal ability in iPS cells. No change in nuclear location. 1 Publication1
Mutagenesisi215K → R: No change in sumoylation; when associated with R-244. Loss of sumoylation. No change in nuclear localization; when associated with R-118 and R-244. 1 Publication1
Mutagenesisi244K → R: No change in sumoylation. No change in sumoylation; when associated with R-215. Loss of sumoylation; when associated with R-118 and R-215. No change in nuclear localization; when associated with R-118 and R-215. 1 Publication1
Mutagenesisi347S → A: Absence of phosphorylation. Reduced protein degradation. Reduced self-renewal ability in ES cells. No change in FBXW4 binding. Loss of FBXW8 binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007491 – 352POU domain, class 5, transcription factor 1Add BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei106Phosphoserine; by MAPKBy similarity1
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)3 Publications
Modified residuei228PhosphothreonineBy similarity1
Modified residuei229PhosphoserineBy similarity1
Modified residuei282PhosphoserineBy similarity1
Modified residuei347Phosphoserine; by MAPK8 and MAPK91 Publication1

Post-translational modificationi

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.3 Publications
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.2 Publications
ERK1/2-mediated phosphorylation at Ser-106 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-228 and Ser-229 decrease DNA-binding and alters ability to activate transcription (By similarity). JNK1/2-mediated phosphorylation at Ser-347 promotes proteasomal degradation (PubMed:29153991).By similarity1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP20263
PeptideAtlasiP20263
PRIDEiP20263

PTM databases

iPTMnetiP20263
PhosphoSitePlusiP20263

Expressioni

Tissue specificityi

Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.2 Publications

Developmental stagei

Down-regulated during differentiation to endoderm and mesoderm.1 Publication

Inductioni

Repressed by retinoic acid (RA).3 Publications

Gene expression databases

BgeeiENSMUSG00000024406 Expressed in 75 organ(s), highest expression level in morula
CleanExiMM_POU5F1
ExpressionAtlasiP20263 baseline and differential
GenevisibleiP20263 MM

Interactioni

Subunit structurei

Interacts with PKM. Interacts with WWP2 (By similarity). Interacts with UBE2I and ZSCAN10 (PubMed:17496161, PubMed:19740739). Interacts with PCGF1 (By similarity). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (PubMed:18662995). Interacts with ZNF322 (PubMed:24550733). Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-347 (PubMed:29153991). Interacts (when phosphorylated on Ser-347) with FBXW8 (PubMed:29153991). Interacts with FBXW4 (PubMed:29153991).By similarity5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202313, 304 interactors
DIPiDIP-29931N
IntActiP20263, 141 interactors
MINTiP20263
STRINGi10090.ENSMUSP00000025271

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20263
SMRiP20263
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20263

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 205POU-specificPROSITE-ProRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni173 – 179DNA bindingCombined sources1 Publication7
Regioni186 – 189DNA bindingCombined sources1 Publication4

Domaini

The POU-specific domain mediates interaction with PKM.By similarity

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000089941
HOVERGENiHBG057998
InParanoidiP20263
KOiK09367
OMAiLENMFLQ
OrthoDBiEOG091G0U0S
PhylomeDBiP20263
TreeFamiTF316413

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR015585 POU_dom_5
PANTHERiPTHR11636:SF86 PTHR11636:SF86, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

P20263-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGHLASDFA FSPPPGGGDG SAGLEPGWVD PRTWLSFQGP PGGPGIGPGS
60 70 80 90 100
EVLGISPCPP AYEFCGGMAY CGPQVGLGLV PQVGVETLQP EGQAGARVES
110 120 130 140 150
NSEGTSSEPC ADRPNAVKLE KVEPTPEESQ DMKALQKELE QFAKLLKQKR
160 170 180 190 200
ITLGYTQADV GLTLGVLFGK VFSQTTICRF EALQLSLKNM CKLRPLLEKW
210 220 230 240 250
VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET MFLKCPKPSL
260 270 280 290 300
QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG
310 320 330 340 350
GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH

SN
Length:352
Mass (Da):38,216
Last modified:February 1, 1991 - v1
Checksum:i757E41DF52286714
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E2JL30E2JL30_MOUSE
Octamer-binding transcription facto...
Pou5f1 Oct-3/4
132Annotation score:
G3UZG9G3UZG9_MOUSE
Oct-3/4 protein
Pou5f1 Oct-3/4
157Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 28Missing in CAA36682 (PubMed:1690859).CuratedAdd BLAST28
Sequence conflicti29V → M in CAA36682 (PubMed:1690859).Curated1
Sequence conflicti31P → S in AAA39844 (PubMed:1915274).Curated1
Sequence conflicti31P → S in AAB19896 (PubMed:1915274).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34381 mRNA Translation: AAA39844.1 Sequence problems.
X52437 mRNA Translation: CAA36682.1
S58426
, S58422, S58423, S58424, S58425 Genomic DNA Translation: AAB19896.1
BC068268 mRNA Translation: AAH68268.1
CCDSiCCDS37600.1
PIRiA34672
S17313
RefSeqiNP_038661.2, NM_013633.3
UniGeneiMm.17031

Genome annotation databases

EnsembliENSMUST00000025271; ENSMUSP00000025271; ENSMUSG00000024406
GeneIDi18999
KEGGimmu:18999
UCSCiuc008chu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34381 mRNA Translation: AAA39844.1 Sequence problems.
X52437 mRNA Translation: CAA36682.1
S58426
, S58422, S58423, S58424, S58425 Genomic DNA Translation: AAB19896.1
BC068268 mRNA Translation: AAH68268.1
CCDSiCCDS37600.1
PIRiA34672
S17313
RefSeqiNP_038661.2, NM_013633.3
UniGeneiMm.17031

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OCPNMR-A217-282[»]
3L1PX-ray2.80A/B131-282[»]
ProteinModelPortaliP20263
SMRiP20263
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202313, 304 interactors
DIPiDIP-29931N
IntActiP20263, 141 interactors
MINTiP20263
STRINGi10090.ENSMUSP00000025271

PTM databases

iPTMnetiP20263
PhosphoSitePlusiP20263

Proteomic databases

PaxDbiP20263
PeptideAtlasiP20263
PRIDEiP20263

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025271; ENSMUSP00000025271; ENSMUSG00000024406
GeneIDi18999
KEGGimmu:18999
UCSCiuc008chu.2 mouse

Organism-specific databases

CTDi5460
MGIiMGI:101893 Pou5f1

Phylogenomic databases

eggNOGiKOG3802 Eukaryota
ENOG410XQ7X LUCA
GeneTreeiENSGT00760000118935
HOGENOMiHOG000089941
HOVERGENiHBG057998
InParanoidiP20263
KOiK09367
OMAiLENMFLQ
OrthoDBiEOG091G0U0S
PhylomeDBiP20263
TreeFamiTF316413

Enzyme and pathway databases

ReactomeiR-MMU-452723 Transcriptional regulation of pluripotent stem cells

Miscellaneous databases

EvolutionaryTraceiP20263
PROiPR:P20263
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024406 Expressed in 75 organ(s), highest expression level in morula
CleanExiMM_POU5F1
ExpressionAtlasiP20263 baseline and differential
GenevisibleiP20263 MM

Family and domain databases

CDDicd00086 homeodomain, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017970 Homeobox_CS
IPR001356 Homeobox_dom
IPR010982 Lambda_DNA-bd_dom_sf
IPR013847 POU
IPR000327 POU_dom
IPR015585 POU_dom_5
PANTHERiPTHR11636:SF86 PTHR11636:SF86, 1 hit
PfamiView protein in Pfam
PF00046 Homeobox, 1 hit
PF00157 Pou, 1 hit
PRINTSiPR00028 POUDOMAIN
SMARTiView protein in SMART
SM00389 HOX, 1 hit
SM00352 POU, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
SSF47413 SSF47413, 1 hit
PROSITEiView protein in PROSITE
PS00027 HOMEOBOX_1, 1 hit
PS50071 HOMEOBOX_2, 1 hit
PS00035 POU_1, 1 hit
PS00465 POU_2, 1 hit
PS51179 POU_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPO5F1_MOUSE
AccessioniPrimary (citable) accession number: P20263
Secondary accession number(s): Q63843
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 183 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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