UniProtKB - P20241 (NRG_DROME)
Protein
Neuroglian
Gene
Nrg
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
The long isoform may play a role in neural and glial cell adhesion in the developing embryo. The short isoform may be a more general cell adhesion molecule involved in other tissues and imaginal disk morphogenesis. Vital for embryonic development. Essential for septate junctions. Septate junctions, which are the equivalent of vertebrates tight junctions, are characterized by regular arrays of transverse structures that span the intermembrane space and form a physical barrier to diffusion. Required for the blood-brain barrier formation.1 Publication
GO - Molecular functioni
- calcium ion binding Source: FlyBase
- cell adhesion molecule binding Source: FlyBase
GO - Biological processi
- axon ensheathment Source: FlyBase
- axon extension Source: FlyBase
- axonogenesis Source: FlyBase
- cell adhesion involved in heart morphogenesis Source: FlyBase
- cell-cell adhesion Source: FlyBase
- central complex development Source: FlyBase
- dendrite morphogenesis Source: FlyBase
- establishment of glial blood-brain barrier Source: FlyBase
- female courtship behavior Source: FlyBase
- imaginal disc morphogenesis Source: UniProtKB
- male courtship behavior Source: FlyBase
- melanotic encapsulation of foreign target Source: FlyBase
- motor neuron axon guidance Source: FlyBase
- mushroom body development Source: FlyBase
- nerve maturation Source: FlyBase
- neuron cell-cell adhesion Source: UniProtKB
- photoreceptor cell axon guidance Source: FlyBase
- regulation of female receptivity Source: FlyBase
- regulation of tube size, open tracheal system Source: FlyBase
- septate junction assembly Source: FlyBase
- synapse organization Source: FlyBase
Keywordsi
Molecular function | Developmental protein |
Biological process | Cell adhesion |
Names & Taxonomyi
Protein namesi | Recommended name: Neuroglian |
Gene namesi | Name:Nrg ORF Names:CG1634 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0264975, Nrg |
Subcellular locationi
Plasma membrane
Other locations
Plasma Membrane
- integral component of plasma membrane Source: FlyBase
- plasma membrane Source: UniProtKB
Other locations
- axon Source: FlyBase
- bicellular tight junction Source: UniProtKB-SubCell
- dendrite Source: FlyBase
- filopodium Source: FlyBase
- neuronal cell body Source: FlyBase
- pleated septate junction Source: FlyBase
- septate junction Source: FlyBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 1138 | ExtracellularSequence analysisAdd BLAST | 1115 | |
Transmembranei | 1139 – 1154 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 1155 – 1302 | CytoplasmicSequence analysisAdd BLAST | 148 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 1 PublicationAdd BLAST | 23 | |
ChainiPRO_0000015055 | 24 – 1302 | NeuroglianAdd BLAST | 1279 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 59 ↔ 111 | PROSITE-ProRule annotation | ||
Disulfide bondi | 155 ↔ 212 | By similarity | ||
Glycosylationi | 182 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 198 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 268 ↔ 317 | By similarity | ||
Disulfide bondi | 360 ↔ 410 | By similarity | ||
Glycosylationi | 411 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Glycosylationi | 448 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 625 ↔ 706 | PROSITE-ProRule annotation1 Publication | ||
Glycosylationi | 652 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 683 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 821 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1125 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P20241 |
PTM databases
iPTMneti | P20241 |
Expressioni
Tissue specificityi
Long isoform is restricted to surface of neurons and glia in the developing nervous system and the short isoform to other non-neuronal tissues.1 Publication
Gene expression databases
Bgeei | FBgn0264975, Expressed in oviduct (Drosophila) and 50 other tissues |
ExpressionAtlasi | P20241, baseline and differential |
Genevisiblei | P20241, DM |
Interactioni
Subunit structurei
Forms a complex with Nrx and Cont.
1 PublicationGO - Molecular functioni
- cell adhesion molecule binding Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 58251, 24 interactors |
DIPi | DIP-22412N |
IntActi | P20241, 79 interactors |
STRINGi | 7227.FBpp0297081 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P20241 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20241 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 133 | Ig-like C2-type 1Add BLAST | 105 | |
Domaini | 134 – 225 | Ig-like C2-type 2Add BLAST | 92 | |
Domaini | 245 – 330 | Ig-like C2-type 3Add BLAST | 86 | |
Domaini | 339 – 426 | Ig-like C2-type 4Add BLAST | 88 | |
Domaini | 432 – 524 | Ig-like C2-type 5Add BLAST | 93 | |
Domaini | 521 – 610 | Ig-like C2-type 6Add BLAST | 90 | |
Domaini | 614 – 711 | Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 716 – 813 | Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 818 – 915 | Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 916 – 1017 | Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST | 102 | |
Domaini | 1021 – 1119 | Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST | 99 |
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3513, Eukaryota |
InParanoidi | P20241 |
OMAi | WEPQGDN |
PhylomeDBi | P20241 |
Family and domain databases
CDDi | cd00063, FN3, 5 hits |
Gene3Di | 2.60.40.10, 11 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013151, Immunoglobulin IPR026966, Neurofascin/L1/NrCAM_C |
Pfami | View protein in Pfam PF13882, Bravo_FIGEY, 1 hit PF00041, fn3, 3 hits PF07679, I-set, 2 hits PF00047, ig, 2 hits |
SMARTi | View protein in SMART SM00060, FN3, 5 hits SM00409, IG, 6 hits SM00408, IGc2, 6 hits |
SUPFAMi | SSF48726, SSF48726, 6 hits SSF49265, SSF49265, 3 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 5 hits PS50835, IG_LIKE, 6 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform B (identifier: P20241-1) [UniParc]FASTAAdd to basket
Also known as: 180 kDa form
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MWRQSTILAA LLVALLCAGS AESKGNRPPR ITKQPAPGEL LFKVAQQNKE
60 70 80 90 100
SDNPFIIECE ADGQPEPEYS WIKNGKKFDW QAYDNRMLRQ PGRGTLVITI
110 120 130 140 150
PKDEDRGHYQ CFASNEFGTA TSNSVYVRKA ELNAFKDEAA KTLEAVEGEP
160 170 180 190 200
FMLKCAAPDG FPSPTVNWMI QESIDGSIKS INNSRMTLDP EGNLWFSNVT
210 220 230 240 250
REDASSDFYY ACSATSVFRS EYKIGNKVLL DVKQMGVSAS QNKHPPVRQY
260 270 280 290 300
VSRRQSLALR GKRMELFCIY GGTPLPQTVW SKDGQRIQWS DRITQGHYGK
310 320 330 340 350
SLVIRQTNFD DAGTYTCDVS NGVGNAQSFS IILNVNSVPY FTKEPEIATA
360 370 380 390 400
AEDEEVVFEC RAAGVPEPKI SWIHNGKPIE QSTPNPRRTV TDNTIRIINL
410 420 430 440 450
VKGDTGNYGC NATNSLGYVY KDVYLNVQAE PPTISEAPAA VSTVDGRNVT
460 470 480 490 500
IKCRVNGSPK PLVKWLRASN WLTGGRYNVQ ANGDLEIQDV TFSDAGKYTC
510 520 530 540 550
YAQNKFGEIQ ADGSLVVKEH TRITQEPQNY EVAAGQSATF RCNEAHDDTL
560 570 580 590 600
EIEIDWWKDG QSIDFEAQPR FVKTNDNSLT IAKTMELDSG EYTCVARTRL
610 620 630 640 650
DEATARANLI VQDVPNAPKL TGITCQADKA EIHWEQQGDN RSPILHYTIQ
660 670 680 690 700
FNTSFTPASW DAAYEKVPNT DSSFVVQMSP WANYTFRVIA FNKIGASPPS
710 720 730 740 750
AHSDSCTTQP DVPFKNPDNV VGQGTEPNNL VISWTPMPEI EHNAPNFHYY
760 770 780 790 800
VSWKRDIPAA AWENNNIFDW RQNNIVIADQ PTFVKYLIKV VAINDRGESN
810 820 830 840 850
VAAEEVVGYS GEDRPLDAPT NFTMRQITSS TSGYMAWTPV SEESVRGHFK
860 870 880 890 900
GYKIQTWTEN EGEEGLREIH VKGDTHNALV TQFKPDSKNY ARILAYNGRF
910 920 930 940 950
NGPPSAVIDF DTPEGVPSPV QGLDAYPLGS SAFMLHWKKP LYPNGKLTGY
960 970 980 990 1000
KIYYEEVKES YVGERREYDP HITDPRVTRM KMAGLKPNSK YRISITATTK
1010 1020 1030 1040 1050
MGEGSEHYIE KTTLKDAVNV APATPSFSWE QLPSDNGLAK FRINWLPSTE
1060 1070 1080 1090 1100
GHPGTHFFTM HRIKGETQWI RENEEKNSDY QEVGGLDPET AYEFRVVSVD
1110 1120 1130 1140 1150
GHFNTESATQ EIDTNTVEGP IMVANETVAN AGWFIGMMLA LAFIIILFII
1160 1170 1180 1190 1200
ICIIRRNRGG KYDVHDRELA NGRRDYPEEG GFHEYSQPLD NKSAGRQSVS
1210 1220 1230 1240 1250
SANKPGVESD TDSMAEYGDG DTGQFTEDGS FIGQYVPGKL QPPVSPQPLN
1260 1270 1280 1290 1300
NSAAAHQAAP TAGGSGAAGS AAAAGASGGA SSAGGAAASN GGAAAGAVAT
YV
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM9NEZ5 | M9NEZ5_DROME | Neuroglian, isoform G | Nrg ceb, CT4318, Dmel\CG1634, fs(1)M72, ibx | 1,309 | Annotation score: | ||
E1JJF8 | E1JJF8_DROME | Neuroglian, isoform E | Nrg ceb, CT4318, Dmel\CG1634, fs(1)M72, ibx | 1,302 | Annotation score: | ||
E1JJF9 | E1JJF9_DROME | Neuroglian, isoform D | Nrg ceb, CT4318, Dmel\CG1634, fs(1)M72, ibx | 1,239 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 85 – 86 | NR → KP in AAC28613 (PubMed:9666073).Curated | 2 | |
Sequence conflicti | 85 – 86 | NR → KP in AAC28614 (PubMed:9666073).Curated | 2 | |
Sequence conflicti | 1232 | Missing in ABE01201 (Ref. 7) Curated | 1 | |
Sequence conflicti | 1282 | Missing in CAA53823 (PubMed:1693086).Curated | 1 | |
Sequence conflicti | 1299 | A → V in ABE01201 (Ref. 7) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002601 | 1224 – 1239 | QFTED…YVPGK → MNEDGSFIGQYGRKGL in isoform A. 3 PublicationsAdd BLAST | 16 | |
Alternative sequenceiVSP_002602 | 1240 – 1302 | Missing in isoform A. 3 PublicationsAdd BLAST | 63 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28231 mRNA Translation: AAA28728.2 AF050085, AF050084 Genomic DNA Translation: AAC28613.2 AF050085, AF050084 Genomic DNA Translation: AAC28614.2 AE014298 Genomic DNA Translation: AAF46387.1 AE014298 Genomic DNA Translation: AAN09236.1 AE014298 Genomic DNA Translation: AAS65287.1 AY058284 mRNA Translation: AAL13513.1 BT024971 mRNA Translation: ABE01201.1 X76243 mRNA Translation: CAA53822.1 X76244 mRNA Translation: CAA53823.1 |
PIRi | A32579 |
RefSeqi | NP_001162704.1, NM_001169233.2 [P20241-2] NP_001162705.1, NM_001169234.3 [P20241-1] NP_001162706.1, NM_001169235.1 [P20241-2] NP_001259336.1, NM_001272407.2 [P20241-2] NP_001259337.1, NM_001272408.1 [P20241-1] NP_511090.1, NM_078535.3 [P20241-2] NP_727274.1, NM_167160.2 [P20241-1] NP_996380.1, NM_206657.4 [P20241-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0071207; FBpp0071154; FBgn0264975 [P20241-2] FBtr0071208; FBpp0071155; FBgn0264975 [P20241-1] FBtr0071209; FBpp0089326; FBgn0264975 [P20241-2] FBtr0301762; FBpp0290976; FBgn0264975 [P20241-2] FBtr0301763; FBpp0290977; FBgn0264975 [P20241-1] FBtr0301764; FBpp0290978; FBgn0264975 [P20241-2] FBtr0333522; FBpp0305702; FBgn0264975 [P20241-2] FBtr0333523; FBpp0305703; FBgn0264975 [P20241-1] |
GeneIDi | 31792 |
KEGGi | dme:Dmel_CG1634 |
UCSCi | CG1634-RC, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M28231 mRNA Translation: AAA28728.2 AF050085, AF050084 Genomic DNA Translation: AAC28613.2 AF050085, AF050084 Genomic DNA Translation: AAC28614.2 AE014298 Genomic DNA Translation: AAF46387.1 AE014298 Genomic DNA Translation: AAN09236.1 AE014298 Genomic DNA Translation: AAS65287.1 AY058284 mRNA Translation: AAL13513.1 BT024971 mRNA Translation: ABE01201.1 X76243 mRNA Translation: CAA53822.1 X76244 mRNA Translation: CAA53823.1 |
PIRi | A32579 |
RefSeqi | NP_001162704.1, NM_001169233.2 [P20241-2] NP_001162705.1, NM_001169234.3 [P20241-1] NP_001162706.1, NM_001169235.1 [P20241-2] NP_001259336.1, NM_001272407.2 [P20241-2] NP_001259337.1, NM_001272408.1 [P20241-1] NP_511090.1, NM_078535.3 [P20241-2] NP_727274.1, NM_167160.2 [P20241-1] NP_996380.1, NM_206657.4 [P20241-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CFB | X-ray | 2.00 | A | 610-814 | [»] | |
SMRi | P20241 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 58251, 24 interactors |
DIPi | DIP-22412N |
IntActi | P20241, 79 interactors |
STRINGi | 7227.FBpp0297081 |
PTM databases
iPTMneti | P20241 |
Proteomic databases
PaxDbi | P20241 |
Genome annotation databases
EnsemblMetazoai | FBtr0071207; FBpp0071154; FBgn0264975 [P20241-2] FBtr0071208; FBpp0071155; FBgn0264975 [P20241-1] FBtr0071209; FBpp0089326; FBgn0264975 [P20241-2] FBtr0301762; FBpp0290976; FBgn0264975 [P20241-2] FBtr0301763; FBpp0290977; FBgn0264975 [P20241-1] FBtr0301764; FBpp0290978; FBgn0264975 [P20241-2] FBtr0333522; FBpp0305702; FBgn0264975 [P20241-2] FBtr0333523; FBpp0305703; FBgn0264975 [P20241-1] |
GeneIDi | 31792 |
KEGGi | dme:Dmel_CG1634 |
UCSCi | CG1634-RC, d. melanogaster |
Organism-specific databases
CTDi | 31792 |
FlyBasei | FBgn0264975, Nrg |
Phylogenomic databases
eggNOGi | KOG3513, Eukaryota |
InParanoidi | P20241 |
OMAi | WEPQGDN |
PhylomeDBi | P20241 |
Miscellaneous databases
BioGRID-ORCSi | 31792, 0 hits in 3 CRISPR screens |
ChiTaRSi | Nrg, fly |
EvolutionaryTracei | P20241 |
GenomeRNAii | 31792 |
PROi | PR:P20241 |
Gene expression databases
Bgeei | FBgn0264975, Expressed in oviduct (Drosophila) and 50 other tissues |
ExpressionAtlasi | P20241, baseline and differential |
Genevisiblei | P20241, DM |
Family and domain databases
CDDi | cd00063, FN3, 5 hits |
Gene3Di | 2.60.40.10, 11 hits |
InterProi | View protein in InterPro IPR003961, FN3_dom IPR036116, FN3_sf IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR013098, Ig_I-set IPR003599, Ig_sub IPR003598, Ig_sub2 IPR013151, Immunoglobulin IPR026966, Neurofascin/L1/NrCAM_C |
Pfami | View protein in Pfam PF13882, Bravo_FIGEY, 1 hit PF00041, fn3, 3 hits PF07679, I-set, 2 hits PF00047, ig, 2 hits |
SMARTi | View protein in SMART SM00060, FN3, 5 hits SM00409, IG, 6 hits SM00408, IGc2, 6 hits |
SUPFAMi | SSF48726, SSF48726, 6 hits SSF49265, SSF49265, 3 hits |
PROSITEi | View protein in PROSITE PS50853, FN3, 5 hits PS50835, IG_LIKE, 6 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NRG_DROME | |
Accessioni | P20241Primary (citable) accession number: P20241 Secondary accession number(s): A4V452 Q9V3X0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 215 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references