UniProtKB - P20231 (TRYB2_HUMAN)
Protein
Tryptase beta-2
Gene
TPSB2
Organism
Homo sapiens (Human)
Status
Functioni
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.By similarity
Catalytic activityi
- Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin. EC:3.4.21.59
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 74 | Charge relay system | 1 | |
Active sitei | 121 | Charge relay system | 1 | |
Active sitei | 224 | Charge relay system | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: GO_Central
- serine-type peptidase activity Source: ProtInc
GO - Biological processi
- proteolysis Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Enzyme and pathway databases
BRENDAi | 3.4.21.59 2681 |
Protein family/group databases
MEROPSi | S01.015 |
Names & Taxonomyi
Protein namesi | Recommended name: Tryptase beta-2 (EC:3.4.21.59)Short name: Tryptase-2 Alternative name(s): Tryptase II |
Gene namesi | Name:TPSB2 Synonyms:TPS2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000197253.13 |
HGNCi | HGNC:14120 TPSB2 |
MIMi | 191081 gene |
neXtProti | NX_P20231 |
Subcellular locationi
Extracellular region or secreted
Note: Released from the secretory granules upon mast cell activation.
Extracellular region or secreted
- collagen-containing extracellular matrix Source: BHF-UCL
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 64499 7177 |
OpenTargetsi | ENSG00000172236 |
PharmGKBi | PA36698 |
Chemistry databases
DrugBanki | DB04654 4-PIPERIDIN-4-YLBUTANAL DB04764 [4-(3-AMINOMETHYL-PHENYL)-PIPERIDIN-1-YL]-(5-PHENETHYL- PYRIDIN-3-YL)-METHANONE DB02018 Amido Phenyl Pyruvic Acid |
Polymorphism and mutation databases
BioMutai | TPSB2 |
DMDMi | 116242830 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
PropeptideiPRO_0000027481 | 19 – 30 | Activation peptideAdd BLAST | 12 | |
ChainiPRO_0000027482 | 31 – 275 | Tryptase beta-2Add BLAST | 245 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 59 ↔ 75 | |||
Modified residuei | 97 | PhosphotyrosineBy similarity | 1 | |
Disulfide bondi | 155 ↔ 230 | |||
Disulfide bondi | 188 ↔ 211 | |||
Disulfide bondi | 220 ↔ 248 | |||
Glycosylationi | 233 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, ZymogenProteomic databases
jPOSTi | P20231 |
PeptideAtlasi | P20231 |
PRIDEi | P20231 |
ProteomicsDBi | 53734 |
PTM databases
GlyConnecti | 1862 |
iPTMneti | P20231 |
PhosphoSitePlusi | P20231 |
Expressioni
Gene expression databases
Bgeei | ENSG00000197253 Expressed in 85 organ(s), highest expression level in esophagogastric junction muscularis propria |
ExpressionAtlasi | P20231 baseline and differential |
Genevisiblei | P20231 HS |
Organism-specific databases
HPAi | HPA049153 HPA049554 |
Interactioni
Subunit structurei
Homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors eg: fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6.1 Publication
Protein-protein interaction databases
BioGridi | 113029, 2 interactors 122201, 16 interactors |
ComplexPortali | CPX-3805 Tryptase beta-2 complex |
IntActi | P20231, 2 interactors |
Chemistry databases
BindingDBi | P20231 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A0L | X-ray | 3.00 | A/B/C/D | 31-274 | [»] | |
1AAO | model | - | A | 31-275 | [»] | |
2BM2 | X-ray | 2.20 | A/B/C/D | 31-275 | [»] | |
2FPZ | X-ray | 2.00 | A/B/C/D | 31-275 | [»] | |
2FS8 | X-ray | 2.50 | A/B/C/D | 31-275 | [»] | |
2FS9 | X-ray | 2.30 | A/B/C/D | 31-275 | [»] | |
2FWW | X-ray | 2.25 | A/B/C/D | 31-275 | [»] | |
2FXR | X-ray | 2.50 | A/B/C/D | 31-275 | [»] | |
2GDD | X-ray | 2.35 | A/B/C/D | 31-275 | [»] | |
2ZA5 | X-ray | 2.30 | A/B/C/D | 31-275 | [»] | |
3V7T | X-ray | 2.09 | A/B/C/D | 31-275 | [»] | |
ProteinModelPortali | P20231 | |||||
SMRi | P20231 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20231 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 31 – 272 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 242 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627 Eukaryota COG5640 LUCA |
HOVERGENi | HBG013304 |
InParanoidi | P20231 |
KOi | K01340 |
OrthoDBi | 1341682at2759 |
PhylomeDBi | P20231 |
Family and domain databases
CDDi | cd00190 Tryp_SPc, 1 hit |
InterProi | View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER |
Pfami | View protein in Pfam PF00089 Trypsin, 1 hit |
PRINTSi | PR00722 CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020 Tryp_SPc, 1 hit |
SUPFAMi | SSF50494 SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P20231-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLNLLLLALP VLASRAYAAP APGQALQRVG IVGGQEAPRS KWPWQVSLRV
60 70 80 90 100
HGPYWMHFCG GSLIHPQWVL TAAHCVGPDV KDLAALRVQL REQHLYYQDQ
110 120 130 140 150
LLPVSRIIVH PQFYTAQIGA DIALLELEEP VNVSSHVHTV TLPPASETFP
160 170 180 190 200
PGMPCWVTGW GDVDNDERLP PPFPLKQVKV PIMENHICDA KYHLGAYTGD
210 220 230 240 250
DVRIVRDDML CAGNTRRDSC QGDSGGPLVC KVNGTWLQAG VVSWGEGCAQ
260 270
PNRPGIYTRV TYYLDWIHHY VPKKP
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087X1U0 | A0A087X1U0_HUMAN | Tryptase beta-2 | TPSB2 | 292 | Annotation score: | ||
A0A087WUI4 | A0A087WUI4_HUMAN | Tryptase beta-2 | TPSB2 | 282 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 132 | N → K in AAA51843 (PubMed:2203827).Curated | 1 | |
Sequence conflicti | 132 | N → K in AAD13876 (PubMed:8434231).Curated | 1 | |
Sequence conflicti | 132 | N → K in AAD17858 (PubMed:9920877).Curated | 1 | |
Sequence conflicti | 132 | N → K in ACZ98911 (PubMed:19748655).Curated | 1 | |
Sequence conflicti | 132 | N → K in AAH29356 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 132 | N → K in AAA36779 (PubMed:2187193).Curated | 1 | |
Sequence conflicti | 132 | N → K in AAA36780 (PubMed:2187193).Curated | 1 | |
Sequence conflicti | 141 | T → A in ACZ98913 (PubMed:19748655).Curated | 1 | |
Sequence conflicti | 240 | G → D in ACZ98913 (PubMed:19748655).Curated | 1 |
Polymorphismi
There are two alleles; beta-II and beta-III. Beta-II is the most commun allele. There are two forms of the beta-III allele, a short and a long form. The short form (also named frameshifted form) is carried by 23% and 19% of individuals of European and African ancestry but 0% of Asian subjects. In the human genome reference version GRCh37/hg19, the allele beta-III short form is represented.5 Publications
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_012104 | 51 – 53 | HGP → RDR in beta-III. | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37488 mRNA Translation: AAA51843.1 S55551 mRNA Translation: AAD13876.1 AF099143 Genomic DNA Translation: AAD17859.2 AF099145 Genomic DNA Translation: AAD17857.1 AF099146 Genomic DNA Translation: AAD17858.1 FJ931117 Genomic DNA Translation: ACZ98911.1 FJ931120 mRNA Translation: ACZ98913.1 AC120498 Genomic DNA No translation available. BC029356 mRNA Translation: AAH29356.1 M33492 mRNA Translation: AAA36779.1 M33493 mRNA Translation: AAA36780.1 |
PIRi | B35863 C35863 |
RefSeqi | NP_003285.2, NM_003294.3 NP_077078.5, NM_024164.5 |
UniGenei | Hs.405479 Hs.592982 |
Genome annotation databases
Ensembli | ENST00000606293; ENSP00000482743; ENSG00000197253 |
GeneIDi | 64499 7177 |
KEGGi | hsa:64499 hsa:7177 |
UCSCi | uc032dnv.1 human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37488 mRNA Translation: AAA51843.1 S55551 mRNA Translation: AAD13876.1 AF099143 Genomic DNA Translation: AAD17859.2 AF099145 Genomic DNA Translation: AAD17857.1 AF099146 Genomic DNA Translation: AAD17858.1 FJ931117 Genomic DNA Translation: ACZ98911.1 FJ931120 mRNA Translation: ACZ98913.1 AC120498 Genomic DNA No translation available. BC029356 mRNA Translation: AAH29356.1 M33492 mRNA Translation: AAA36779.1 M33493 mRNA Translation: AAA36780.1 |
PIRi | B35863 C35863 |
RefSeqi | NP_003285.2, NM_003294.3 NP_077078.5, NM_024164.5 |
UniGenei | Hs.405479 Hs.592982 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A0L | X-ray | 3.00 | A/B/C/D | 31-274 | [»] | |
1AAO | model | - | A | 31-275 | [»] | |
2BM2 | X-ray | 2.20 | A/B/C/D | 31-275 | [»] | |
2FPZ | X-ray | 2.00 | A/B/C/D | 31-275 | [»] | |
2FS8 | X-ray | 2.50 | A/B/C/D | 31-275 | [»] | |
2FS9 | X-ray | 2.30 | A/B/C/D | 31-275 | [»] | |
2FWW | X-ray | 2.25 | A/B/C/D | 31-275 | [»] | |
2FXR | X-ray | 2.50 | A/B/C/D | 31-275 | [»] | |
2GDD | X-ray | 2.35 | A/B/C/D | 31-275 | [»] | |
2ZA5 | X-ray | 2.30 | A/B/C/D | 31-275 | [»] | |
3V7T | X-ray | 2.09 | A/B/C/D | 31-275 | [»] | |
ProteinModelPortali | P20231 | |||||
SMRi | P20231 | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 113029, 2 interactors 122201, 16 interactors |
ComplexPortali | CPX-3805 Tryptase beta-2 complex |
IntActi | P20231, 2 interactors |
Chemistry databases
BindingDBi | P20231 |
DrugBanki | DB04654 4-PIPERIDIN-4-YLBUTANAL DB04764 [4-(3-AMINOMETHYL-PHENYL)-PIPERIDIN-1-YL]-(5-PHENETHYL- PYRIDIN-3-YL)-METHANONE DB02018 Amido Phenyl Pyruvic Acid |
Protein family/group databases
MEROPSi | S01.015 |
PTM databases
GlyConnecti | 1862 |
iPTMneti | P20231 |
PhosphoSitePlusi | P20231 |
Polymorphism and mutation databases
BioMutai | TPSB2 |
DMDMi | 116242830 |
Proteomic databases
jPOSTi | P20231 |
PeptideAtlasi | P20231 |
PRIDEi | P20231 |
ProteomicsDBi | 53734 |
Protocols and materials databases
DNASUi | 64499 7177 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000606293; ENSP00000482743; ENSG00000197253 |
GeneIDi | 64499 7177 |
KEGGi | hsa:64499 hsa:7177 |
UCSCi | uc032dnv.1 human |
Organism-specific databases
CTDi | 64499 7177 |
DisGeNETi | 64499 7177 |
EuPathDBi | HostDB:ENSG00000197253.13 |
GeneCardsi | TPSB2 |
HGNCi | HGNC:14120 TPSB2 |
HPAi | HPA049153 HPA049554 |
MIMi | 191081 gene |
neXtProti | NX_P20231 |
OpenTargetsi | ENSG00000172236 |
PharmGKBi | PA36698 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627 Eukaryota COG5640 LUCA |
HOVERGENi | HBG013304 |
InParanoidi | P20231 |
KOi | K01340 |
OrthoDBi | 1341682at2759 |
PhylomeDBi | P20231 |
Enzyme and pathway databases
BRENDAi | 3.4.21.59 2681 |
Miscellaneous databases
EvolutionaryTracei | P20231 |
GeneWikii | TPSAB1 TPSB2 |
PROi | PR:P20231 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000197253 Expressed in 85 organ(s), highest expression level in esophagogastric junction muscularis propria |
ExpressionAtlasi | P20231 baseline and differential |
Genevisiblei | P20231 HS |
Family and domain databases
CDDi | cd00190 Tryp_SPc, 1 hit |
InterProi | View protein in InterPro IPR009003 Peptidase_S1_PA IPR001314 Peptidase_S1A IPR001254 Trypsin_dom IPR018114 TRYPSIN_HIS IPR033116 TRYPSIN_SER |
Pfami | View protein in Pfam PF00089 Trypsin, 1 hit |
PRINTSi | PR00722 CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020 Tryp_SPc, 1 hit |
SUPFAMi | SSF50494 SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240 TRYPSIN_DOM, 1 hit PS00134 TRYPSIN_HIS, 1 hit PS00135 TRYPSIN_SER, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | TRYB2_HUMAN | |
Accessioni | P20231Primary (citable) accession number: P20231 Secondary accession number(s): D2E6S0 Q9UQI7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | October 17, 2006 | |
Last modified: | February 13, 2019 | |
This is version 177 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Complete proteome, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations