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Protein

TATA-box-binding protein

Gene

TBP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II (PubMed:2374612, PubMed:2363050, PubMed:2194289, PubMed:9836642, PubMed:27193682). Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III (PubMed:26638071). Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription (PubMed:15970593). The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.Curated7 Publications

GO - Molecular functioni

  • aryl hydrocarbon receptor binding Source: CAFA
  • core promoter binding Source: CAFA
  • enzyme binding Source: BHF-UCL
  • proximal promoter sequence-specific DNA binding Source: UniProtKB
  • repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase III transcription factor activity, sequence-specific DNA binding Source: UniProtKB
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl
  • RNA polymerase II repressing transcription factor binding Source: Ensembl
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription factor activity, TBP-class protein binding, involved in preinitiation complex assembly Source: CAFA
  • TFIIB-class transcription factor binding Source: CAFA
  • transcription factor binding Source: UniProtKB
  • transcription regulatory region DNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processHost-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-427359 SIRT1 negatively regulates rRNA expression
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-5250924 B-WICH complex positively regulates rRNA expression
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-HSA-9018519 Estrogen-dependent gene expression
SignaLinkiP20226
SIGNORiP20226

Names & Taxonomyi

Protein namesi
Recommended name:
TATA-box-binding protein
Alternative name(s):
TATA sequence-binding protein
TATA-binding factor2 Publications
TATA-box factor
Transcription initiation factor TFIID TBP subunit
Gene namesi
Name:TBP
Synonyms:GTF2D1, TF2D, TFIID1 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112592.12
HGNCiHGNC:11588 TBP
MIMi600075 gene
neXtProtiNX_P20226

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Spinocerebellar ataxia 17 (SCA17)3 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionSpinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
See also OMIM:607136

Keywords - Diseasei

Disease mutation, Neurodegeneration, Spinocerebellar ataxia

Organism-specific databases

DisGeNETi6908
GeneReviewsiTBP
MalaCardsiTBP
MIMi607136 phenotype
OpenTargetsiENSG00000112592
Orphaneti98759 Spinocerebellar ataxia type 17
PharmGKBiPA36352

Polymorphism and mutation databases

BioMutaiTBP
DMDMi1351223

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539561 – 339TATA-box-binding proteinAdd BLAST339

Proteomic databases

EPDiP20226
MaxQBiP20226
PaxDbiP20226
PeptideAtlasiP20226
PRIDEiP20226
ProteomicsDBi53733

PTM databases

iPTMnetiP20226
PhosphoSitePlusiP20226
SwissPalmiP20226

Expressioni

Tissue specificityi

Widely expressed, with levels highest in the testis and ovary.1 Publication

Gene expression databases

BgeeiENSG00000112592
CleanExiHS_TBP
ExpressionAtlasiP20226 baseline and differential
GenevisibleiP20226 HS

Organism-specific databases

HPAiCAB009442
HPA049805

Interactioni

Subunit structurei

Binds DNA as monomer (PubMed:2374612, PubMed:2194289). Belongs to the TFIID complex together with the TBP-associated factors (TAFs) (PubMed:9836642, PubMed:27007846). Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 (PubMed:27007846). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D (PubMed:7801123). Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive (PubMed:7801123). Interacts with TAF1A, TAF1B and TAF1C (PubMed:7801123). Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3 (PubMed:10567404, PubMed:10391676, PubMed:11461703). Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4 (PubMed:10196196, PubMed:12621023). Interacts with UTF1 (PubMed:9748258). Interacts with BRF2; this interaction promotes recruitment of BRF2 to TATA box-containing promoters (PubMed:11564744, PubMed:26638071). Interacts with UBTF (PubMed:7982918). Interacts with GPBP1 (By similarity). Interacts with CITED2 (By similarity). Interacts with ATF7IP (Probable). Interacts with LLPH (By similarity). Interacts with HSF1 (via transactivation domain) (PubMed:11005381).By similarity19 Publications
(Microbial infection) Interacts with HIV-1 Tat.3 Publications
(Microbial infection) Interacts with herpes simplex virus 1 ICP4.1 Publication
(Microbial infection) Interacts with human adenovirus E1A protein; this interaction probably disrupts the TBP-TATA complex.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • aryl hydrocarbon receptor binding Source: CAFA
  • enzyme binding Source: BHF-UCL
  • repressing transcription factor binding Source: BHF-UCL
  • RNA polymerase II repressing transcription factor binding Source: Ensembl
  • TFIIB-class transcription factor binding Source: CAFA
  • transcription factor binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112771, 173 interactors
ComplexPortaliCPX-915 General transcription factor complex TFIID
CPX-930 General transcription factor complex TFIID, TAF4B variant
CORUMiP20226
DIPiDIP-1078N
IntActiP20226, 87 interactors
MINTiP20226
STRINGi9606.ENSP00000230354

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi164 – 173Combined sources10
Helixi180 – 186Combined sources7
Beta strandi190 – 192Combined sources3
Turni194 – 196Combined sources3
Beta strandi198 – 204Combined sources7
Turni205 – 208Combined sources4
Beta strandi209 – 213Combined sources5
Beta strandi217 – 222Combined sources6
Helixi227 – 244Combined sources18
Beta strandi251 – 263Combined sources13
Helixi270 – 276Combined sources7
Turni277 – 280Combined sources4
Turni285 – 287Combined sources3
Beta strandi289 – 295Combined sources7
Turni296 – 299Combined sources4
Beta strandi300 – 304Combined sources5
Beta strandi308 – 313Combined sources6
Helixi318 – 333Combined sources16

3D structure databases

ProteinModelPortaliP20226
SMRiP20226
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20226

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati165 – 2411Add BLAST77
Repeati255 – 3322Add BLAST78

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi55 – 95Poly-GlnAdd BLAST41

Sequence similaritiesi

Belongs to the TBP family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3302 Eukaryota
COG2101 LUCA
GeneTreeiENSGT00410000025389
HOGENOMiHOG000105164
HOVERGENiHBG044997
InParanoidiP20226
KOiK03120
OMAiMMPYGSG
OrthoDBiEOG091G0ENY
PhylomeDBiP20226
TreeFamiTF300102

Family and domain databases

CDDicd04516 TBP_eukaryotes, 1 hit
Gene3Di3.30.310.10, 2 hits
HAMAPiMF_00408 TATA_bind_prot_arch, 1 hit
InterProiView protein in InterPro
IPR000814 TBP
IPR030491 TBP_CS
IPR012295 TBP_dom_sf
IPR033710 TBP_eukaryotic
PANTHERiPTHR10126 PTHR10126, 1 hit
PfamiView protein in Pfam
PF00352 TBP, 2 hits
PRINTSiPR00686 TIFACTORIID
PROSITEiView protein in PROSITE
PS00351 TFIID, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P20226-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS
60 70 80 90 100
ILEEQQRQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQAVAAA
110 120 130 140 150
AVQQSTSQQA TQGTSGQAPQ LFHSQTLTTA PLPGTTPLYP SPMTPMTPIT
160 170 180 190 200
PATPASESSG IVPQLQNIVS TVNLGCKLDL KTIALRARNA EYNPKRFAAV
210 220 230 240 250
IMRIREPRTT ALIFSSGKMV CTGAKSEEQS RLAARKYARV VQKLGFPAKF
260 270 280 290 300
LDFKIQNMVG SCDVKFPIRL EGLVLTHQQF SSYEPELFPG LIYRMIKPRI
310 320 330
VLLIFVSGKV VLTGAKVRAE IYEAFENIYP ILKGFRKTT
Length:339
Mass (Da):37,698
Last modified:February 1, 1996 - v2
Checksum:iA61A578D972B970B
GO
Isoform 2 (identifier: P20226-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: No experimental confirmation available.
Show »
Length:319
Mass (Da):35,657
Checksum:i18CF7E0A7240DC5D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti95Missing in BAG65425 (PubMed:14702039).Curated1
Sequence conflicti149I → T in BAG65425 (PubMed:14702039).Curated1
Sequence conflicti187A → R in AAC03409 (PubMed:2194289).Curated1

Polymorphismi

The poly-Gln region of TBP is highly polymorphic (25 to 42 repeats) in normal individuals and is expanded to about 47-63 repeats in spinocerebellar ataxia 17 (SCA17) patients.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01698792 – 95Missing 1 Publication4

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0454881 – 20Missing in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54993 mRNA Translation: CAA38736.1
M55654 mRNA Translation: AAA36731.1
M34960 mRNA Translation: AAC03409.1
AK304648 mRNA Translation: BAG65425.1
CR456776 mRNA Translation: CAG33057.1
AL031259 Genomic DNA No translation available.
CCDSiCCDS5315.1 [P20226-1]
CCDS55077.1 [P20226-2]
PIRiA34830 TWHU2D
RefSeqiNP_001165556.1, NM_001172085.1 [P20226-2]
NP_003185.1, NM_003194.4 [P20226-1]
UniGeneiHs.590872

Genome annotation databases

EnsembliENST00000230354; ENSP00000230354; ENSG00000112592 [P20226-1]
ENST00000392092; ENSP00000375942; ENSG00000112592 [P20226-1]
ENST00000540980; ENSP00000442132; ENSG00000112592 [P20226-2]
GeneIDi6908
KEGGihsa:6908
UCSCiuc003qxt.4 human [P20226-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism, Triplet repeat expansion

Similar proteinsi

Entry informationi

Entry nameiTBP_HUMAN
AccessioniPrimary (citable) accession number: P20226
Secondary accession number(s): B4E3B3
, F5H869, Q16845, Q6IBM6, Q9UC02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1996
Last modified: July 18, 2018
This is version 215 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

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